Enantiomeric separations by capillary electrophoresis: Theoretical method to determine optimum chiral selector concentration

Autores
Lancioni, Carlina; Keunchkarian, Sonia; Castells, Cecilia Beatriz Marta; Gagliardi, Leonardo Gabriel
Año de publicación
2018
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
A method to optimize the ligand concentration [S] in the background electrolyte of capillary electrophoresis separations is presented. It is based on the use of a model which predicts apparent electrophoretic mobilities as a function of ligand concentration (expressed as p[S] = −log[S]). This model is employed to compose the expression of a recently proposed criterion to qualify separations in electrophoresis. Two strategies to find the optimum p[S], leading to the best separation of all compounds, are explained: 1.- a graphical method using a windows map depicting the single separation criteria between all possible combination of compounds by pairs, and 2.- an analytical method where an extended multicriterion optimization function is composed and optimum p[S] is found by mathematical maximization. The procedure is applied to a hard-to-separate model system: enantiomeric separations of racemic mixtures. 2-Hydroxypropyl-β-cyclodextrin was chosen as a model ligand, and four pharmaceutical drugs as model analytes. In order to demonstrate the performance of the procedure, results of electrophoretic separations obtained at p[S] found as optimum are compared with separations obtained at p[S] values slightly higher and lower than the optimum.
Fil: Lancioni, Carlina. Provincia de Buenos Aires. Gobernación. Comisión de Investigaciones Científicas; Argentina. Universidad Nacional de La Plata. Facultad de Ciencias Exactas; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Keunchkarian, Sonia. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Provincia de Buenos Aires. Gobernación. Comisión de Investigaciones Científicas; Argentina. Universidad Nacional de La Plata. Facultad de Ciencias Exactas; Argentina
Fil: Castells, Cecilia Beatriz Marta. Provincia de Buenos Aires. Gobernación. Comisión de Investigaciones Científicas; Argentina. Universidad Nacional de La Plata. Facultad de Ciencias Exactas; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Gagliardi, Leonardo Gabriel. Universidad Nacional de La Plata. Facultad de Ciencias Exactas; Argentina. Provincia de Buenos Aires. Gobernación. Comisión de Investigaciones Científicas; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Materia
CAPILLARY ELECTROPHORESIS
CHIRAL SEPARATION
CYCLODEXTRIN
MULTICRITERION OPTIMIZATION FUNCTION
SEPARATION OPTIMIZATION
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/99696
Acceder
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repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Enantiomeric separations by capillary electrophoresis: Theoretical method to determine optimum chiral selector concentrationLancioni, CarlinaKeunchkarian, SoniaCastells, Cecilia Beatriz MartaGagliardi, Leonardo GabrielCAPILLARY ELECTROPHORESISCHIRAL SEPARATIONCYCLODEXTRINMULTICRITERION OPTIMIZATION FUNCTIONSEPARATION OPTIMIZATIONhttps://purl.org/becyt/ford/1.4https://purl.org/becyt/ford/1A method to optimize the ligand concentration [S] in the background electrolyte of capillary electrophoresis separations is presented. It is based on the use of a model which predicts apparent electrophoretic mobilities as a function of ligand concentration (expressed as p[S] = −log[S]). This model is employed to compose the expression of a recently proposed criterion to qualify separations in electrophoresis. Two strategies to find the optimum p[S], leading to the best separation of all compounds, are explained: 1.- a graphical method using a windows map depicting the single separation criteria between all possible combination of compounds by pairs, and 2.- an analytical method where an extended multicriterion optimization function is composed and optimum p[S] is found by mathematical maximization. The procedure is applied to a hard-to-separate model system: enantiomeric separations of racemic mixtures. 2-Hydroxypropyl-β-cyclodextrin was chosen as a model ligand, and four pharmaceutical drugs as model analytes. In order to demonstrate the performance of the procedure, results of electrophoretic separations obtained at p[S] found as optimum are compared with separations obtained at p[S] values slightly higher and lower than the optimum.Fil: Lancioni, Carlina. Provincia de Buenos Aires. Gobernación. Comisión de Investigaciones Científicas; Argentina. Universidad Nacional de La Plata. Facultad de Ciencias Exactas; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Keunchkarian, Sonia. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Provincia de Buenos Aires. Gobernación. Comisión de Investigaciones Científicas; Argentina. Universidad Nacional de La Plata. Facultad de Ciencias Exactas; ArgentinaFil: Castells, Cecilia Beatriz Marta. Provincia de Buenos Aires. Gobernación. Comisión de Investigaciones Científicas; Argentina. Universidad Nacional de La Plata. Facultad de Ciencias Exactas; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Gagliardi, Leonardo Gabriel. Universidad Nacional de La Plata. Facultad de Ciencias Exactas; Argentina. Provincia de Buenos Aires. Gobernación. Comisión de Investigaciones Científicas; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaElsevier Science2018-03info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/99696Lancioni, Carlina; Keunchkarian, Sonia; Castells, Cecilia Beatriz Marta; Gagliardi, Leonardo Gabriel; Enantiomeric separations by capillary electrophoresis: Theoretical method to determine optimum chiral selector concentration; Elsevier Science; Journal of Chromatography - A; 1539; 3-2018; 71-770021-9673CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.1016/j.chroma.2018.01.002info:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/pii/S0021967318300013info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-nd/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-03T09:46:19Zoai:ri.conicet.gov.ar:11336/99696instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-03 09:46:19.723CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Enantiomeric separations by capillary electrophoresis: Theoretical method to determine optimum chiral selector concentration
title Enantiomeric separations by capillary electrophoresis: Theoretical method to determine optimum chiral selector concentration
spellingShingle Enantiomeric separations by capillary electrophoresis: Theoretical method to determine optimum chiral selector concentration
Lancioni, Carlina
CAPILLARY ELECTROPHORESIS
CHIRAL SEPARATION
CYCLODEXTRIN
MULTICRITERION OPTIMIZATION FUNCTION
SEPARATION OPTIMIZATION
title_short Enantiomeric separations by capillary electrophoresis: Theoretical method to determine optimum chiral selector concentration
title_full Enantiomeric separations by capillary electrophoresis: Theoretical method to determine optimum chiral selector concentration
title_fullStr Enantiomeric separations by capillary electrophoresis: Theoretical method to determine optimum chiral selector concentration
title_full_unstemmed Enantiomeric separations by capillary electrophoresis: Theoretical method to determine optimum chiral selector concentration
title_sort Enantiomeric separations by capillary electrophoresis: Theoretical method to determine optimum chiral selector concentration
dc.creator.none.fl_str_mv Lancioni, Carlina
Keunchkarian, Sonia
Castells, Cecilia Beatriz Marta
Gagliardi, Leonardo Gabriel
author Lancioni, Carlina
author_facet Lancioni, Carlina
Keunchkarian, Sonia
Castells, Cecilia Beatriz Marta
Gagliardi, Leonardo Gabriel
author_role author
author2 Keunchkarian, Sonia
Castells, Cecilia Beatriz Marta
Gagliardi, Leonardo Gabriel
author2_role author
author
author
dc.subject.none.fl_str_mv CAPILLARY ELECTROPHORESIS
CHIRAL SEPARATION
CYCLODEXTRIN
MULTICRITERION OPTIMIZATION FUNCTION
SEPARATION OPTIMIZATION
topic CAPILLARY ELECTROPHORESIS
CHIRAL SEPARATION
CYCLODEXTRIN
MULTICRITERION OPTIMIZATION FUNCTION
SEPARATION OPTIMIZATION
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.4
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv A method to optimize the ligand concentration [S] in the background electrolyte of capillary electrophoresis separations is presented. It is based on the use of a model which predicts apparent electrophoretic mobilities as a function of ligand concentration (expressed as p[S] = −log[S]). This model is employed to compose the expression of a recently proposed criterion to qualify separations in electrophoresis. Two strategies to find the optimum p[S], leading to the best separation of all compounds, are explained: 1.- a graphical method using a windows map depicting the single separation criteria between all possible combination of compounds by pairs, and 2.- an analytical method where an extended multicriterion optimization function is composed and optimum p[S] is found by mathematical maximization. The procedure is applied to a hard-to-separate model system: enantiomeric separations of racemic mixtures. 2-Hydroxypropyl-β-cyclodextrin was chosen as a model ligand, and four pharmaceutical drugs as model analytes. In order to demonstrate the performance of the procedure, results of electrophoretic separations obtained at p[S] found as optimum are compared with separations obtained at p[S] values slightly higher and lower than the optimum.
Fil: Lancioni, Carlina. Provincia de Buenos Aires. Gobernación. Comisión de Investigaciones Científicas; Argentina. Universidad Nacional de La Plata. Facultad de Ciencias Exactas; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Keunchkarian, Sonia. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Provincia de Buenos Aires. Gobernación. Comisión de Investigaciones Científicas; Argentina. Universidad Nacional de La Plata. Facultad de Ciencias Exactas; Argentina
Fil: Castells, Cecilia Beatriz Marta. Provincia de Buenos Aires. Gobernación. Comisión de Investigaciones Científicas; Argentina. Universidad Nacional de La Plata. Facultad de Ciencias Exactas; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Gagliardi, Leonardo Gabriel. Universidad Nacional de La Plata. Facultad de Ciencias Exactas; Argentina. Provincia de Buenos Aires. Gobernación. Comisión de Investigaciones Científicas; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
description A method to optimize the ligand concentration [S] in the background electrolyte of capillary electrophoresis separations is presented. It is based on the use of a model which predicts apparent electrophoretic mobilities as a function of ligand concentration (expressed as p[S] = −log[S]). This model is employed to compose the expression of a recently proposed criterion to qualify separations in electrophoresis. Two strategies to find the optimum p[S], leading to the best separation of all compounds, are explained: 1.- a graphical method using a windows map depicting the single separation criteria between all possible combination of compounds by pairs, and 2.- an analytical method where an extended multicriterion optimization function is composed and optimum p[S] is found by mathematical maximization. The procedure is applied to a hard-to-separate model system: enantiomeric separations of racemic mixtures. 2-Hydroxypropyl-β-cyclodextrin was chosen as a model ligand, and four pharmaceutical drugs as model analytes. In order to demonstrate the performance of the procedure, results of electrophoretic separations obtained at p[S] found as optimum are compared with separations obtained at p[S] values slightly higher and lower than the optimum.
publishDate 2018
dc.date.none.fl_str_mv 2018-03
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/99696
Lancioni, Carlina; Keunchkarian, Sonia; Castells, Cecilia Beatriz Marta; Gagliardi, Leonardo Gabriel; Enantiomeric separations by capillary electrophoresis: Theoretical method to determine optimum chiral selector concentration; Elsevier Science; Journal of Chromatography - A; 1539; 3-2018; 71-77
0021-9673
CONICET Digital
CONICET
url http://hdl.handle.net/11336/99696
identifier_str_mv Lancioni, Carlina; Keunchkarian, Sonia; Castells, Cecilia Beatriz Marta; Gagliardi, Leonardo Gabriel; Enantiomeric separations by capillary electrophoresis: Theoretical method to determine optimum chiral selector concentration; Elsevier Science; Journal of Chromatography - A; 1539; 3-2018; 71-77
0021-9673
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.1016/j.chroma.2018.01.002
info:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/pii/S0021967318300013
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Elsevier Science
publisher.none.fl_str_mv Elsevier Science
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.13397

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