The Expression of Sphingosine Kinase-1 in Head and Neck Carcinoma

Autores
Facchinetti, Maria Marta; Gandini, Norberto Ariel; Fermento, María Eugenia; Sterin, Norma Beatriz; Ji, Youngmi; Patel, Vyomesh; Gutkind, J. Silvio; Rivadulla, Maria G.; Curino, Alejandro Carlos
Año de publicación
2010
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Sphingosine kinase-1 (SPHK1) modulates the proliferation, apoptosis and differentiation of keratinocytes through the regulation of ceramide and sphingosine-1-phosphate levels. However, studies on the expression of SPHK1 in human head and neck squamous cell carcinoma (HNSCC) specimens are lacking. Therefore, the aim of the present work was to evaluate SPHK1 expression in human primary HNSCCs and to correlate the results with clinical and anatomopathological parameters. We investigated the expression of this protein by immunohistochemistry performed in tissue microarrays of HNSCC and in an independent cohort of 37 paraffin-embedded specimens. SPHK1 expression was further validated by real-time PCR performed on laser capture-microdissected tissue samples. The positive rate of SPHK1 protein in the cancerous tissues was significantly higher (74%) than that in the nontumor oral tissues (23%), and malignant tissues showed stronger immunoreactivity for SPHK1 than normal matching samples. These results were confirmed by real-time PCR quantification of SPHK1 mRNA. Interestingly, the positive expression of SPHK1 was associated with shorter patient survival time (Kaplan-Meier survival curves) and with the loss of p21 expression. Taken together, these results demonstrate that SPHK1 is upregulated in HNSCC and provide clues of the role SPHK1 might play in tumor progression.
Fil: Facchinetti, Maria Marta. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; Argentina
Fil: Gandini, Norberto Ariel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; Argentina
Fil: Fermento, María Eugenia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; Argentina
Fil: Sterin, Norma Beatriz. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Departamento de Biología Celular e Histología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Ji, Youngmi. National Institutes of Health; Estados Unidos
Fil: Patel, Vyomesh. National Institutes of Health; Estados Unidos
Fil: Gutkind, J. Silvio. National Institutes of Health; Estados Unidos
Fil: Rivadulla, Maria G.. Hospital Municipal de Agudos "Dr. Leónidas Lucero"; Argentina
Fil: Curino, Alejandro Carlos. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; Argentina
Materia
Sphingosine Kinase
Oral Cancer
Sphingolipids
Tissue Microarray
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/18232
Acceder
id CONICETDig_64a2f8f60e846f21b1e439e462ee80c4
oai_identifier_str oai:ri.conicet.gov.ar:11336/18232
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling The Expression of Sphingosine Kinase-1 in Head and Neck CarcinomaFacchinetti, Maria MartaGandini, Norberto ArielFermento, María EugeniaSterin, Norma BeatrizJi, YoungmiPatel, VyomeshGutkind, J. SilvioRivadulla, Maria G.Curino, Alejandro CarlosSphingosine KinaseOral CancerSphingolipidsTissue Microarrayhttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1Sphingosine kinase-1 (SPHK1) modulates the proliferation, apoptosis and differentiation of keratinocytes through the regulation of ceramide and sphingosine-1-phosphate levels. However, studies on the expression of SPHK1 in human head and neck squamous cell carcinoma (HNSCC) specimens are lacking. Therefore, the aim of the present work was to evaluate SPHK1 expression in human primary HNSCCs and to correlate the results with clinical and anatomopathological parameters. We investigated the expression of this protein by immunohistochemistry performed in tissue microarrays of HNSCC and in an independent cohort of 37 paraffin-embedded specimens. SPHK1 expression was further validated by real-time PCR performed on laser capture-microdissected tissue samples. The positive rate of SPHK1 protein in the cancerous tissues was significantly higher (74%) than that in the nontumor oral tissues (23%), and malignant tissues showed stronger immunoreactivity for SPHK1 than normal matching samples. These results were confirmed by real-time PCR quantification of SPHK1 mRNA. Interestingly, the positive expression of SPHK1 was associated with shorter patient survival time (Kaplan-Meier survival curves) and with the loss of p21 expression. Taken together, these results demonstrate that SPHK1 is upregulated in HNSCC and provide clues of the role SPHK1 might play in tumor progression.Fil: Facchinetti, Maria Marta. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; ArgentinaFil: Gandini, Norberto Ariel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; ArgentinaFil: Fermento, María Eugenia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; ArgentinaFil: Sterin, Norma Beatriz. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Departamento de Biología Celular e Histología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Ji, Youngmi. National Institutes of Health; Estados UnidosFil: Patel, Vyomesh. National Institutes of Health; Estados UnidosFil: Gutkind, J. Silvio. National Institutes of Health; Estados UnidosFil: Rivadulla, Maria G.. Hospital Municipal de Agudos "Dr. Leónidas Lucero"; ArgentinaFil: Curino, Alejandro Carlos. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; ArgentinaKarger2010-10info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/18232Facchinetti, Maria Marta; Gandini, Norberto Ariel; Fermento, María Eugenia; Sterin, Norma Beatriz; Ji, Youngmi; et al.; The Expression of Sphingosine Kinase-1 in Head and Neck Carcinoma; Karger; Cells Tissues Organs; 192; 5; 10-2010; 314-3241422-6405CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.1159/000318173info:eu-repo/semantics/altIdentifier/url/https://www.karger.com/Article/FullText/318173info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T10:39:21Zoai:ri.conicet.gov.ar:11336/18232instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 10:39:21.662CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv The Expression of Sphingosine Kinase-1 in Head and Neck Carcinoma
title The Expression of Sphingosine Kinase-1 in Head and Neck Carcinoma
spellingShingle The Expression of Sphingosine Kinase-1 in Head and Neck Carcinoma
Facchinetti, Maria Marta
Sphingosine Kinase
Oral Cancer
Sphingolipids
Tissue Microarray
title_short The Expression of Sphingosine Kinase-1 in Head and Neck Carcinoma
title_full The Expression of Sphingosine Kinase-1 in Head and Neck Carcinoma
title_fullStr The Expression of Sphingosine Kinase-1 in Head and Neck Carcinoma
title_full_unstemmed The Expression of Sphingosine Kinase-1 in Head and Neck Carcinoma
title_sort The Expression of Sphingosine Kinase-1 in Head and Neck Carcinoma
dc.creator.none.fl_str_mv Facchinetti, Maria Marta
Gandini, Norberto Ariel
Fermento, María Eugenia
Sterin, Norma Beatriz
Ji, Youngmi
Patel, Vyomesh
Gutkind, J. Silvio
Rivadulla, Maria G.
Curino, Alejandro Carlos
author Facchinetti, Maria Marta
author_facet Facchinetti, Maria Marta
Gandini, Norberto Ariel
Fermento, María Eugenia
Sterin, Norma Beatriz
Ji, Youngmi
Patel, Vyomesh
Gutkind, J. Silvio
Rivadulla, Maria G.
Curino, Alejandro Carlos
author_role author
author2 Gandini, Norberto Ariel
Fermento, María Eugenia
Sterin, Norma Beatriz
Ji, Youngmi
Patel, Vyomesh
Gutkind, J. Silvio
Rivadulla, Maria G.
Curino, Alejandro Carlos
author2_role author
author
author
author
author
author
author
author
dc.subject.none.fl_str_mv Sphingosine Kinase
Oral Cancer
Sphingolipids
Tissue Microarray
topic Sphingosine Kinase
Oral Cancer
Sphingolipids
Tissue Microarray
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv Sphingosine kinase-1 (SPHK1) modulates the proliferation, apoptosis and differentiation of keratinocytes through the regulation of ceramide and sphingosine-1-phosphate levels. However, studies on the expression of SPHK1 in human head and neck squamous cell carcinoma (HNSCC) specimens are lacking. Therefore, the aim of the present work was to evaluate SPHK1 expression in human primary HNSCCs and to correlate the results with clinical and anatomopathological parameters. We investigated the expression of this protein by immunohistochemistry performed in tissue microarrays of HNSCC and in an independent cohort of 37 paraffin-embedded specimens. SPHK1 expression was further validated by real-time PCR performed on laser capture-microdissected tissue samples. The positive rate of SPHK1 protein in the cancerous tissues was significantly higher (74%) than that in the nontumor oral tissues (23%), and malignant tissues showed stronger immunoreactivity for SPHK1 than normal matching samples. These results were confirmed by real-time PCR quantification of SPHK1 mRNA. Interestingly, the positive expression of SPHK1 was associated with shorter patient survival time (Kaplan-Meier survival curves) and with the loss of p21 expression. Taken together, these results demonstrate that SPHK1 is upregulated in HNSCC and provide clues of the role SPHK1 might play in tumor progression.
Fil: Facchinetti, Maria Marta. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; Argentina
Fil: Gandini, Norberto Ariel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; Argentina
Fil: Fermento, María Eugenia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; Argentina
Fil: Sterin, Norma Beatriz. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Departamento de Biología Celular e Histología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Ji, Youngmi. National Institutes of Health; Estados Unidos
Fil: Patel, Vyomesh. National Institutes of Health; Estados Unidos
Fil: Gutkind, J. Silvio. National Institutes of Health; Estados Unidos
Fil: Rivadulla, Maria G.. Hospital Municipal de Agudos "Dr. Leónidas Lucero"; Argentina
Fil: Curino, Alejandro Carlos. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; Argentina
description Sphingosine kinase-1 (SPHK1) modulates the proliferation, apoptosis and differentiation of keratinocytes through the regulation of ceramide and sphingosine-1-phosphate levels. However, studies on the expression of SPHK1 in human head and neck squamous cell carcinoma (HNSCC) specimens are lacking. Therefore, the aim of the present work was to evaluate SPHK1 expression in human primary HNSCCs and to correlate the results with clinical and anatomopathological parameters. We investigated the expression of this protein by immunohistochemistry performed in tissue microarrays of HNSCC and in an independent cohort of 37 paraffin-embedded specimens. SPHK1 expression was further validated by real-time PCR performed on laser capture-microdissected tissue samples. The positive rate of SPHK1 protein in the cancerous tissues was significantly higher (74%) than that in the nontumor oral tissues (23%), and malignant tissues showed stronger immunoreactivity for SPHK1 than normal matching samples. These results were confirmed by real-time PCR quantification of SPHK1 mRNA. Interestingly, the positive expression of SPHK1 was associated with shorter patient survival time (Kaplan-Meier survival curves) and with the loss of p21 expression. Taken together, these results demonstrate that SPHK1 is upregulated in HNSCC and provide clues of the role SPHK1 might play in tumor progression.
publishDate 2010
dc.date.none.fl_str_mv 2010-10
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/18232
Facchinetti, Maria Marta; Gandini, Norberto Ariel; Fermento, María Eugenia; Sterin, Norma Beatriz; Ji, Youngmi; et al.; The Expression of Sphingosine Kinase-1 in Head and Neck Carcinoma; Karger; Cells Tissues Organs; 192; 5; 10-2010; 314-324
1422-6405
CONICET Digital
CONICET
url http://hdl.handle.net/11336/18232
identifier_str_mv Facchinetti, Maria Marta; Gandini, Norberto Ariel; Fermento, María Eugenia; Sterin, Norma Beatriz; Ji, Youngmi; et al.; The Expression of Sphingosine Kinase-1 in Head and Neck Carcinoma; Karger; Cells Tissues Organs; 192; 5; 10-2010; 314-324
1422-6405
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.1159/000318173
info:eu-repo/semantics/altIdentifier/url/https://www.karger.com/Article/FullText/318173
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Karger
publisher.none.fl_str_mv Karger
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.070432

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