X-Y sample scanning stage and calibration method suitable for single-molecule detection

Autores
Treegate, Ketvalee; Rasamessard, Areefen; Osotchan, Tanakorn; Hodak, Jose Hector
Año de publicación
2010
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
This paper describes the construction of a positioning device for sample scanning in the x and y directions suitable for single molecule fluorescence experiments. The mechanism uses a simple parallelogram flexure cut out of a single aluminum plate and two amplified piezoelectric actuators of the type used for microscope objective focus adjustment. A displacement range of 75 μm on each axis is obtained. The stage can be used to implement a sample scanning confocal microscope for single molecule spectroscopy applications using either inverted or up-right microscopes. Images with diffraction limited resolution can be obtained with this scanning stage. This is demonstrated by imaging glass beads labeled with the DY475 fluorescent dye and single rhodamine molecules. Micron sized range images of 256 × 256 pixels can be obtained with dwell times down to 0.5 ms/pixel. A novel direct calibration in which the mechanical response obtained from the line profiles for forward and reverse motion is used to account for the hysteresis of the stage. The target molecules are then located within the focus of the laser beam by using its corrected position. The performance of this scanning device and correction technique are demonstrated for the acquisition of fluorescence trajectories of individual rhodamine molecules. © 2010 Published by Elsevier B.V.
Fil: Treegate, Ketvalee. Mahidol University; Tailandia
Fil: Rasamessard, Areefen. Mahidol University; Tailandia
Fil: Osotchan, Tanakorn. Mahidol University; Tailandia
Fil: Hodak, Jose Hector. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Química, Física de los Materiales, Medioambiente y Energía. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Instituto de Química, Física de los Materiales, Medioambiente y Energía; Argentina. Mahidol University; Tailandia
Materia
Fluorescence Spectroscopy
Optical Detection
Scanning Confocal Microscopy
Single Molecule Spectroscopy
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/71414
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/71414
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling X-Y sample scanning stage and calibration method suitable for single-molecule detectionTreegate, KetvaleeRasamessard, AreefenOsotchan, TanakornHodak, Jose HectorFluorescence SpectroscopyOptical DetectionScanning Confocal MicroscopySingle Molecule Spectroscopyhttps://purl.org/becyt/ford/1.4https://purl.org/becyt/ford/1This paper describes the construction of a positioning device for sample scanning in the x and y directions suitable for single molecule fluorescence experiments. The mechanism uses a simple parallelogram flexure cut out of a single aluminum plate and two amplified piezoelectric actuators of the type used for microscope objective focus adjustment. A displacement range of 75 μm on each axis is obtained. The stage can be used to implement a sample scanning confocal microscope for single molecule spectroscopy applications using either inverted or up-right microscopes. Images with diffraction limited resolution can be obtained with this scanning stage. This is demonstrated by imaging glass beads labeled with the DY475 fluorescent dye and single rhodamine molecules. Micron sized range images of 256 × 256 pixels can be obtained with dwell times down to 0.5 ms/pixel. A novel direct calibration in which the mechanical response obtained from the line profiles for forward and reverse motion is used to account for the hysteresis of the stage. The target molecules are then located within the focus of the laser beam by using its corrected position. The performance of this scanning device and correction technique are demonstrated for the acquisition of fluorescence trajectories of individual rhodamine molecules. © 2010 Published by Elsevier B.V.Fil: Treegate, Ketvalee. Mahidol University; TailandiaFil: Rasamessard, Areefen. Mahidol University; TailandiaFil: Osotchan, Tanakorn. Mahidol University; TailandiaFil: Hodak, Jose Hector. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Química, Física de los Materiales, Medioambiente y Energía. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Instituto de Química, Física de los Materiales, Medioambiente y Energía; Argentina. Mahidol University; TailandiaElsevier Science Sa2010-10info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/71414Treegate, Ketvalee; Rasamessard, Areefen; Osotchan, Tanakorn; Hodak, Jose Hector; X-Y sample scanning stage and calibration method suitable for single-molecule detection; Elsevier Science Sa; Sensors and Actuators B: Chemical; 150; 1; 10-2010; 239-2460925-4005CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.1016/j.snb.2010.07.008info:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/pii/S0925400510005836info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-10-22T11:44:02Zoai:ri.conicet.gov.ar:11336/71414instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-10-22 11:44:02.972CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv X-Y sample scanning stage and calibration method suitable for single-molecule detection
title X-Y sample scanning stage and calibration method suitable for single-molecule detection
spellingShingle X-Y sample scanning stage and calibration method suitable for single-molecule detection
Treegate, Ketvalee
Fluorescence Spectroscopy
Optical Detection
Scanning Confocal Microscopy
Single Molecule Spectroscopy
title_short X-Y sample scanning stage and calibration method suitable for single-molecule detection
title_full X-Y sample scanning stage and calibration method suitable for single-molecule detection
title_fullStr X-Y sample scanning stage and calibration method suitable for single-molecule detection
title_full_unstemmed X-Y sample scanning stage and calibration method suitable for single-molecule detection
title_sort X-Y sample scanning stage and calibration method suitable for single-molecule detection
dc.creator.none.fl_str_mv Treegate, Ketvalee
Rasamessard, Areefen
Osotchan, Tanakorn
Hodak, Jose Hector
author Treegate, Ketvalee
author_facet Treegate, Ketvalee
Rasamessard, Areefen
Osotchan, Tanakorn
Hodak, Jose Hector
author_role author
author2 Rasamessard, Areefen
Osotchan, Tanakorn
Hodak, Jose Hector
author2_role author
author
author
dc.subject.none.fl_str_mv Fluorescence Spectroscopy
Optical Detection
Scanning Confocal Microscopy
Single Molecule Spectroscopy
topic Fluorescence Spectroscopy
Optical Detection
Scanning Confocal Microscopy
Single Molecule Spectroscopy
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.4
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv This paper describes the construction of a positioning device for sample scanning in the x and y directions suitable for single molecule fluorescence experiments. The mechanism uses a simple parallelogram flexure cut out of a single aluminum plate and two amplified piezoelectric actuators of the type used for microscope objective focus adjustment. A displacement range of 75 μm on each axis is obtained. The stage can be used to implement a sample scanning confocal microscope for single molecule spectroscopy applications using either inverted or up-right microscopes. Images with diffraction limited resolution can be obtained with this scanning stage. This is demonstrated by imaging glass beads labeled with the DY475 fluorescent dye and single rhodamine molecules. Micron sized range images of 256 × 256 pixels can be obtained with dwell times down to 0.5 ms/pixel. A novel direct calibration in which the mechanical response obtained from the line profiles for forward and reverse motion is used to account for the hysteresis of the stage. The target molecules are then located within the focus of the laser beam by using its corrected position. The performance of this scanning device and correction technique are demonstrated for the acquisition of fluorescence trajectories of individual rhodamine molecules. © 2010 Published by Elsevier B.V.
Fil: Treegate, Ketvalee. Mahidol University; Tailandia
Fil: Rasamessard, Areefen. Mahidol University; Tailandia
Fil: Osotchan, Tanakorn. Mahidol University; Tailandia
Fil: Hodak, Jose Hector. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Química, Física de los Materiales, Medioambiente y Energía. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Instituto de Química, Física de los Materiales, Medioambiente y Energía; Argentina. Mahidol University; Tailandia
description This paper describes the construction of a positioning device for sample scanning in the x and y directions suitable for single molecule fluorescence experiments. The mechanism uses a simple parallelogram flexure cut out of a single aluminum plate and two amplified piezoelectric actuators of the type used for microscope objective focus adjustment. A displacement range of 75 μm on each axis is obtained. The stage can be used to implement a sample scanning confocal microscope for single molecule spectroscopy applications using either inverted or up-right microscopes. Images with diffraction limited resolution can be obtained with this scanning stage. This is demonstrated by imaging glass beads labeled with the DY475 fluorescent dye and single rhodamine molecules. Micron sized range images of 256 × 256 pixels can be obtained with dwell times down to 0.5 ms/pixel. A novel direct calibration in which the mechanical response obtained from the line profiles for forward and reverse motion is used to account for the hysteresis of the stage. The target molecules are then located within the focus of the laser beam by using its corrected position. The performance of this scanning device and correction technique are demonstrated for the acquisition of fluorescence trajectories of individual rhodamine molecules. © 2010 Published by Elsevier B.V.
publishDate 2010
dc.date.none.fl_str_mv 2010-10
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/71414
Treegate, Ketvalee; Rasamessard, Areefen; Osotchan, Tanakorn; Hodak, Jose Hector; X-Y sample scanning stage and calibration method suitable for single-molecule detection; Elsevier Science Sa; Sensors and Actuators B: Chemical; 150; 1; 10-2010; 239-246
0925-4005
CONICET Digital
CONICET
url http://hdl.handle.net/11336/71414
identifier_str_mv Treegate, Ketvalee; Rasamessard, Areefen; Osotchan, Tanakorn; Hodak, Jose Hector; X-Y sample scanning stage and calibration method suitable for single-molecule detection; Elsevier Science Sa; Sensors and Actuators B: Chemical; 150; 1; 10-2010; 239-246
0925-4005
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.1016/j.snb.2010.07.008
info:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/pii/S0925400510005836
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Elsevier Science Sa
publisher.none.fl_str_mv Elsevier Science Sa
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 12.928904

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