X-Y sample scanning stage and calibration method suitable for single-molecule detection
- Autores
- Treegate, Ketvalee; Rasamessard, Areefen; Osotchan, Tanakorn; Hodak, Jose Hector
- Año de publicación
- 2010
- Idioma
- inglés
- Tipo de recurso
- artículo
- Estado
- versión publicada
- Descripción
- This paper describes the construction of a positioning device for sample scanning in the x and y directions suitable for single molecule fluorescence experiments. The mechanism uses a simple parallelogram flexure cut out of a single aluminum plate and two amplified piezoelectric actuators of the type used for microscope objective focus adjustment. A displacement range of 75 μm on each axis is obtained. The stage can be used to implement a sample scanning confocal microscope for single molecule spectroscopy applications using either inverted or up-right microscopes. Images with diffraction limited resolution can be obtained with this scanning stage. This is demonstrated by imaging glass beads labeled with the DY475 fluorescent dye and single rhodamine molecules. Micron sized range images of 256 × 256 pixels can be obtained with dwell times down to 0.5 ms/pixel. A novel direct calibration in which the mechanical response obtained from the line profiles for forward and reverse motion is used to account for the hysteresis of the stage. The target molecules are then located within the focus of the laser beam by using its corrected position. The performance of this scanning device and correction technique are demonstrated for the acquisition of fluorescence trajectories of individual rhodamine molecules. © 2010 Published by Elsevier B.V.
Fil: Treegate, Ketvalee. Mahidol University; Tailandia
Fil: Rasamessard, Areefen. Mahidol University; Tailandia
Fil: Osotchan, Tanakorn. Mahidol University; Tailandia
Fil: Hodak, Jose Hector. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Química, Física de los Materiales, Medioambiente y Energía. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Instituto de Química, Física de los Materiales, Medioambiente y Energía; Argentina. Mahidol University; Tailandia - Materia
-
Fluorescence Spectroscopy
Optical Detection
Scanning Confocal Microscopy
Single Molecule Spectroscopy - Nivel de accesibilidad
- acceso abierto
- Condiciones de uso
- https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
- Repositorio
- Institución
- Consejo Nacional de Investigaciones Científicas y Técnicas
- OAI Identificador
- oai:ri.conicet.gov.ar:11336/71414
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X-Y sample scanning stage and calibration method suitable for single-molecule detectionTreegate, KetvaleeRasamessard, AreefenOsotchan, TanakornHodak, Jose HectorFluorescence SpectroscopyOptical DetectionScanning Confocal MicroscopySingle Molecule Spectroscopyhttps://purl.org/becyt/ford/1.4https://purl.org/becyt/ford/1This paper describes the construction of a positioning device for sample scanning in the x and y directions suitable for single molecule fluorescence experiments. The mechanism uses a simple parallelogram flexure cut out of a single aluminum plate and two amplified piezoelectric actuators of the type used for microscope objective focus adjustment. A displacement range of 75 μm on each axis is obtained. The stage can be used to implement a sample scanning confocal microscope for single molecule spectroscopy applications using either inverted or up-right microscopes. Images with diffraction limited resolution can be obtained with this scanning stage. This is demonstrated by imaging glass beads labeled with the DY475 fluorescent dye and single rhodamine molecules. Micron sized range images of 256 × 256 pixels can be obtained with dwell times down to 0.5 ms/pixel. A novel direct calibration in which the mechanical response obtained from the line profiles for forward and reverse motion is used to account for the hysteresis of the stage. The target molecules are then located within the focus of the laser beam by using its corrected position. The performance of this scanning device and correction technique are demonstrated for the acquisition of fluorescence trajectories of individual rhodamine molecules. © 2010 Published by Elsevier B.V.Fil: Treegate, Ketvalee. Mahidol University; TailandiaFil: Rasamessard, Areefen. Mahidol University; TailandiaFil: Osotchan, Tanakorn. Mahidol University; TailandiaFil: Hodak, Jose Hector. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Química, Física de los Materiales, Medioambiente y Energía. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Instituto de Química, Física de los Materiales, Medioambiente y Energía; Argentina. Mahidol University; TailandiaElsevier Science Sa2010-10info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/71414Treegate, Ketvalee; Rasamessard, Areefen; Osotchan, Tanakorn; Hodak, Jose Hector; X-Y sample scanning stage and calibration method suitable for single-molecule detection; Elsevier Science Sa; Sensors and Actuators B: Chemical; 150; 1; 10-2010; 239-2460925-4005CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.1016/j.snb.2010.07.008info:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/pii/S0925400510005836info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T10:14:04Zoai:ri.conicet.gov.ar:11336/71414instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 10:14:04.475CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse |
dc.title.none.fl_str_mv |
X-Y sample scanning stage and calibration method suitable for single-molecule detection |
title |
X-Y sample scanning stage and calibration method suitable for single-molecule detection |
spellingShingle |
X-Y sample scanning stage and calibration method suitable for single-molecule detection Treegate, Ketvalee Fluorescence Spectroscopy Optical Detection Scanning Confocal Microscopy Single Molecule Spectroscopy |
title_short |
X-Y sample scanning stage and calibration method suitable for single-molecule detection |
title_full |
X-Y sample scanning stage and calibration method suitable for single-molecule detection |
title_fullStr |
X-Y sample scanning stage and calibration method suitable for single-molecule detection |
title_full_unstemmed |
X-Y sample scanning stage and calibration method suitable for single-molecule detection |
title_sort |
X-Y sample scanning stage and calibration method suitable for single-molecule detection |
dc.creator.none.fl_str_mv |
Treegate, Ketvalee Rasamessard, Areefen Osotchan, Tanakorn Hodak, Jose Hector |
author |
Treegate, Ketvalee |
author_facet |
Treegate, Ketvalee Rasamessard, Areefen Osotchan, Tanakorn Hodak, Jose Hector |
author_role |
author |
author2 |
Rasamessard, Areefen Osotchan, Tanakorn Hodak, Jose Hector |
author2_role |
author author author |
dc.subject.none.fl_str_mv |
Fluorescence Spectroscopy Optical Detection Scanning Confocal Microscopy Single Molecule Spectroscopy |
topic |
Fluorescence Spectroscopy Optical Detection Scanning Confocal Microscopy Single Molecule Spectroscopy |
purl_subject.fl_str_mv |
https://purl.org/becyt/ford/1.4 https://purl.org/becyt/ford/1 |
dc.description.none.fl_txt_mv |
This paper describes the construction of a positioning device for sample scanning in the x and y directions suitable for single molecule fluorescence experiments. The mechanism uses a simple parallelogram flexure cut out of a single aluminum plate and two amplified piezoelectric actuators of the type used for microscope objective focus adjustment. A displacement range of 75 μm on each axis is obtained. The stage can be used to implement a sample scanning confocal microscope for single molecule spectroscopy applications using either inverted or up-right microscopes. Images with diffraction limited resolution can be obtained with this scanning stage. This is demonstrated by imaging glass beads labeled with the DY475 fluorescent dye and single rhodamine molecules. Micron sized range images of 256 × 256 pixels can be obtained with dwell times down to 0.5 ms/pixel. A novel direct calibration in which the mechanical response obtained from the line profiles for forward and reverse motion is used to account for the hysteresis of the stage. The target molecules are then located within the focus of the laser beam by using its corrected position. The performance of this scanning device and correction technique are demonstrated for the acquisition of fluorescence trajectories of individual rhodamine molecules. © 2010 Published by Elsevier B.V. Fil: Treegate, Ketvalee. Mahidol University; Tailandia Fil: Rasamessard, Areefen. Mahidol University; Tailandia Fil: Osotchan, Tanakorn. Mahidol University; Tailandia Fil: Hodak, Jose Hector. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Química, Física de los Materiales, Medioambiente y Energía. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Instituto de Química, Física de los Materiales, Medioambiente y Energía; Argentina. Mahidol University; Tailandia |
description |
This paper describes the construction of a positioning device for sample scanning in the x and y directions suitable for single molecule fluorescence experiments. The mechanism uses a simple parallelogram flexure cut out of a single aluminum plate and two amplified piezoelectric actuators of the type used for microscope objective focus adjustment. A displacement range of 75 μm on each axis is obtained. The stage can be used to implement a sample scanning confocal microscope for single molecule spectroscopy applications using either inverted or up-right microscopes. Images with diffraction limited resolution can be obtained with this scanning stage. This is demonstrated by imaging glass beads labeled with the DY475 fluorescent dye and single rhodamine molecules. Micron sized range images of 256 × 256 pixels can be obtained with dwell times down to 0.5 ms/pixel. A novel direct calibration in which the mechanical response obtained from the line profiles for forward and reverse motion is used to account for the hysteresis of the stage. The target molecules are then located within the focus of the laser beam by using its corrected position. The performance of this scanning device and correction technique are demonstrated for the acquisition of fluorescence trajectories of individual rhodamine molecules. © 2010 Published by Elsevier B.V. |
publishDate |
2010 |
dc.date.none.fl_str_mv |
2010-10 |
dc.type.none.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion http://purl.org/coar/resource_type/c_6501 info:ar-repo/semantics/articulo |
format |
article |
status_str |
publishedVersion |
dc.identifier.none.fl_str_mv |
http://hdl.handle.net/11336/71414 Treegate, Ketvalee; Rasamessard, Areefen; Osotchan, Tanakorn; Hodak, Jose Hector; X-Y sample scanning stage and calibration method suitable for single-molecule detection; Elsevier Science Sa; Sensors and Actuators B: Chemical; 150; 1; 10-2010; 239-246 0925-4005 CONICET Digital CONICET |
url |
http://hdl.handle.net/11336/71414 |
identifier_str_mv |
Treegate, Ketvalee; Rasamessard, Areefen; Osotchan, Tanakorn; Hodak, Jose Hector; X-Y sample scanning stage and calibration method suitable for single-molecule detection; Elsevier Science Sa; Sensors and Actuators B: Chemical; 150; 1; 10-2010; 239-246 0925-4005 CONICET Digital CONICET |
dc.language.none.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
info:eu-repo/semantics/altIdentifier/doi/10.1016/j.snb.2010.07.008 info:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/pii/S0925400510005836 |
dc.rights.none.fl_str_mv |
info:eu-repo/semantics/openAccess https://creativecommons.org/licenses/by-nc-sa/2.5/ar/ |
eu_rights_str_mv |
openAccess |
rights_invalid_str_mv |
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/ |
dc.format.none.fl_str_mv |
application/pdf application/pdf |
dc.publisher.none.fl_str_mv |
Elsevier Science Sa |
publisher.none.fl_str_mv |
Elsevier Science Sa |
dc.source.none.fl_str_mv |
reponame:CONICET Digital (CONICET) instname:Consejo Nacional de Investigaciones Científicas y Técnicas |
reponame_str |
CONICET Digital (CONICET) |
collection |
CONICET Digital (CONICET) |
instname_str |
Consejo Nacional de Investigaciones Científicas y Técnicas |
repository.name.fl_str_mv |
CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas |
repository.mail.fl_str_mv |
dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar |
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1844614063700574208 |
score |
13.070432 |