Choline Kinase alpha Expression during RA-induced Neuronal Differentiation: role of C/EBPβ

Autores
Domizi, Pablo Daniel; Aoyama, Chieko; Banchio, Claudia Elena
Año de publicación
2014
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Neuronal differentiation is a complex process characterized by a halt in proliferation and extension of neurites from the cell body. This process is accompanied by changes in gene expression that mediate the redirection leading to neurite formation and function. Acceleration of membrane phospholipids synthesis is associated with neurite elongation, and phosphatidylcholine (PtdCho) is the major membrane phospholipid in mammalian cells. The transcription of two genes in particular encoding key enzymes in the CDP–choline pathway for PtdCho biosynthesis are stimulated; the Chka gene for choline kinase (CK) alpha isoform and the Pcyt1a gene for the CTP: phosphocholine cytidylyltransferase (CCT) alpha isoform. We report that the stimulation of CKα expression during retinoic acid (RA) induced differentiation depends on a promoter region that contains two CCAAT/Enhancerbinding Protein-β (C/EBPβ) sites. We demonstrate that during neuronal differentiation of Neuro-2a cells, RA induces Chka expression by a mechanism that involves ERK½ activation which triggers C/EBPβ expression. Elevated levels of C/EBPβ bind to the Chka proximal promoter (Box1) inducing CKα expression. In addition we identified a downstream sequence named Box2 which together with Box1 is required for the promoter to reach the full induction. This is the first elucidation of the mechanism by which the expression of Chka is coordinately regulated during neuronal differentiation.
Fil: Domizi, Pablo Daniel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Rosario. Instituto de Biología Molecular y Celular de Rosario; Argentina. Universidad Nacional de Rosario; Argentina
Fil: Aoyama, Chieko. Dokkyo Medical University School of Medicine. Department of Biochemistry; Japón
Fil: Banchio, Claudia Elena. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Rosario. Instituto de Biología Molecular y Celular de Rosario; Argentina. Universidad Nacional de Rosario; Argentina
Materia
Chka Gene Promoter
Phosphatidylcholine
Neuritogenesis
Transcriptional Regulation
C/Ebpβ
Lipidos
Colina Kinasa
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/8816
Acceder
id CONICETDig_63efea20c454691b319852e880feafd1
oai_identifier_str oai:ri.conicet.gov.ar:11336/8816
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Choline Kinase alpha Expression during RA-induced Neuronal Differentiation: role of C/EBPβDomizi, Pablo DanielAoyama, ChiekoBanchio, Claudia ElenaChka Gene PromoterPhosphatidylcholineNeuritogenesisTranscriptional RegulationC/EbpβLipidosColina Kinasahttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1Neuronal differentiation is a complex process characterized by a halt in proliferation and extension of neurites from the cell body. This process is accompanied by changes in gene expression that mediate the redirection leading to neurite formation and function. Acceleration of membrane phospholipids synthesis is associated with neurite elongation, and phosphatidylcholine (PtdCho) is the major membrane phospholipid in mammalian cells. The transcription of two genes in particular encoding key enzymes in the CDP–choline pathway for PtdCho biosynthesis are stimulated; the Chka gene for choline kinase (CK) alpha isoform and the Pcyt1a gene for the CTP: phosphocholine cytidylyltransferase (CCT) alpha isoform. We report that the stimulation of CKα expression during retinoic acid (RA) induced differentiation depends on a promoter region that contains two CCAAT/Enhancerbinding Protein-β (C/EBPβ) sites. We demonstrate that during neuronal differentiation of Neuro-2a cells, RA induces Chka expression by a mechanism that involves ERK½ activation which triggers C/EBPβ expression. Elevated levels of C/EBPβ bind to the Chka proximal promoter (Box1) inducing CKα expression. In addition we identified a downstream sequence named Box2 which together with Box1 is required for the promoter to reach the full induction. This is the first elucidation of the mechanism by which the expression of Chka is coordinately regulated during neuronal differentiation.Fil: Domizi, Pablo Daniel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Rosario. Instituto de Biología Molecular y Celular de Rosario; Argentina. Universidad Nacional de Rosario; ArgentinaFil: Aoyama, Chieko. Dokkyo Medical University School of Medicine. Department of Biochemistry; JapónFil: Banchio, Claudia Elena. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Rosario. Instituto de Biología Molecular y Celular de Rosario; Argentina. Universidad Nacional de Rosario; ArgentinaElsevier2014-04info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/8816Domizi, Pablo Daniel; Aoyama, Chieko; Banchio, Claudia Elena; Choline Kinase alpha Expression during RA-induced Neuronal Differentiation: role of C/EBPβ; Elsevier; Biochimica Et Biophysica Acta - Molecular And Cell Biology Of Lipids; 1841; 4; 4-2014; 544-5551388-1981enginfo:eu-repo/semantics/altIdentifier/url/http://www.sciencedirect.com/science/article/pii/S1388198114000122info:eu-repo/semantics/altIdentifier/doi/10.1016/j.bbalip.2014.01.007info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-10-22T11:34:37Zoai:ri.conicet.gov.ar:11336/8816instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-10-22 11:34:37.339CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Choline Kinase alpha Expression during RA-induced Neuronal Differentiation: role of C/EBPβ
title Choline Kinase alpha Expression during RA-induced Neuronal Differentiation: role of C/EBPβ
spellingShingle Choline Kinase alpha Expression during RA-induced Neuronal Differentiation: role of C/EBPβ
Domizi, Pablo Daniel
Chka Gene Promoter
Phosphatidylcholine
Neuritogenesis
Transcriptional Regulation
C/Ebpβ
Lipidos
Colina Kinasa
title_short Choline Kinase alpha Expression during RA-induced Neuronal Differentiation: role of C/EBPβ
title_full Choline Kinase alpha Expression during RA-induced Neuronal Differentiation: role of C/EBPβ
title_fullStr Choline Kinase alpha Expression during RA-induced Neuronal Differentiation: role of C/EBPβ
title_full_unstemmed Choline Kinase alpha Expression during RA-induced Neuronal Differentiation: role of C/EBPβ
title_sort Choline Kinase alpha Expression during RA-induced Neuronal Differentiation: role of C/EBPβ
dc.creator.none.fl_str_mv Domizi, Pablo Daniel
Aoyama, Chieko
Banchio, Claudia Elena
author Domizi, Pablo Daniel
author_facet Domizi, Pablo Daniel
Aoyama, Chieko
Banchio, Claudia Elena
author_role author
author2 Aoyama, Chieko
Banchio, Claudia Elena
author2_role author
author
dc.subject.none.fl_str_mv Chka Gene Promoter
Phosphatidylcholine
Neuritogenesis
Transcriptional Regulation
C/Ebpβ
Lipidos
Colina Kinasa
topic Chka Gene Promoter
Phosphatidylcholine
Neuritogenesis
Transcriptional Regulation
C/Ebpβ
Lipidos
Colina Kinasa
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv Neuronal differentiation is a complex process characterized by a halt in proliferation and extension of neurites from the cell body. This process is accompanied by changes in gene expression that mediate the redirection leading to neurite formation and function. Acceleration of membrane phospholipids synthesis is associated with neurite elongation, and phosphatidylcholine (PtdCho) is the major membrane phospholipid in mammalian cells. The transcription of two genes in particular encoding key enzymes in the CDP–choline pathway for PtdCho biosynthesis are stimulated; the Chka gene for choline kinase (CK) alpha isoform and the Pcyt1a gene for the CTP: phosphocholine cytidylyltransferase (CCT) alpha isoform. We report that the stimulation of CKα expression during retinoic acid (RA) induced differentiation depends on a promoter region that contains two CCAAT/Enhancerbinding Protein-β (C/EBPβ) sites. We demonstrate that during neuronal differentiation of Neuro-2a cells, RA induces Chka expression by a mechanism that involves ERK½ activation which triggers C/EBPβ expression. Elevated levels of C/EBPβ bind to the Chka proximal promoter (Box1) inducing CKα expression. In addition we identified a downstream sequence named Box2 which together with Box1 is required for the promoter to reach the full induction. This is the first elucidation of the mechanism by which the expression of Chka is coordinately regulated during neuronal differentiation.
Fil: Domizi, Pablo Daniel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Rosario. Instituto de Biología Molecular y Celular de Rosario; Argentina. Universidad Nacional de Rosario; Argentina
Fil: Aoyama, Chieko. Dokkyo Medical University School of Medicine. Department of Biochemistry; Japón
Fil: Banchio, Claudia Elena. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Rosario. Instituto de Biología Molecular y Celular de Rosario; Argentina. Universidad Nacional de Rosario; Argentina
description Neuronal differentiation is a complex process characterized by a halt in proliferation and extension of neurites from the cell body. This process is accompanied by changes in gene expression that mediate the redirection leading to neurite formation and function. Acceleration of membrane phospholipids synthesis is associated with neurite elongation, and phosphatidylcholine (PtdCho) is the major membrane phospholipid in mammalian cells. The transcription of two genes in particular encoding key enzymes in the CDP–choline pathway for PtdCho biosynthesis are stimulated; the Chka gene for choline kinase (CK) alpha isoform and the Pcyt1a gene for the CTP: phosphocholine cytidylyltransferase (CCT) alpha isoform. We report that the stimulation of CKα expression during retinoic acid (RA) induced differentiation depends on a promoter region that contains two CCAAT/Enhancerbinding Protein-β (C/EBPβ) sites. We demonstrate that during neuronal differentiation of Neuro-2a cells, RA induces Chka expression by a mechanism that involves ERK½ activation which triggers C/EBPβ expression. Elevated levels of C/EBPβ bind to the Chka proximal promoter (Box1) inducing CKα expression. In addition we identified a downstream sequence named Box2 which together with Box1 is required for the promoter to reach the full induction. This is the first elucidation of the mechanism by which the expression of Chka is coordinately regulated during neuronal differentiation.
publishDate 2014
dc.date.none.fl_str_mv 2014-04
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/8816
Domizi, Pablo Daniel; Aoyama, Chieko; Banchio, Claudia Elena; Choline Kinase alpha Expression during RA-induced Neuronal Differentiation: role of C/EBPβ; Elsevier; Biochimica Et Biophysica Acta - Molecular And Cell Biology Of Lipids; 1841; 4; 4-2014; 544-555
1388-1981
url http://hdl.handle.net/11336/8816
identifier_str_mv Domizi, Pablo Daniel; Aoyama, Chieko; Banchio, Claudia Elena; Choline Kinase alpha Expression during RA-induced Neuronal Differentiation: role of C/EBPβ; Elsevier; Biochimica Et Biophysica Acta - Molecular And Cell Biology Of Lipids; 1841; 4; 4-2014; 544-555
1388-1981
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/http://www.sciencedirect.com/science/article/pii/S1388198114000122
info:eu-repo/semantics/altIdentifier/doi/10.1016/j.bbalip.2014.01.007
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Elsevier
publisher.none.fl_str_mv Elsevier
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
_version_ 1846781973544042496
score 12.982451

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