UV-B radiation on lemons enhances antifungal activity of flavedo extracts against Penicillium digitatum

Autores
Ruiz, Verónica Eugenia; Cerioni, Luciana; Zampini, Iris Catiana; Cuello, Ana Soledad; Isla, Maria Ines; Hilal, Mirna Beatriz; Rapisarda, Viviana Andrea
Año de publicación
2017
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
UV-B radiation (UVBR) treatment, previously standardized in our laboratory, contributes to prevent green mold caused by Penicillium digitatum on postharvest lemons. Here, antifungal activity of flavedo extracts from irradiated and non-irradiated lemons was assayed against P. digitatum; also, compounds that could be responsible for this action were searched. The flavedo extracts from UV-B exposed lemons (UVBLE) exhibited a higher antimicrobial activity than extracts from control lemons (CLE). Conidia treated with UVBLE showed a time dependent inhibition of germination and oxygen consumption, a markedly increase in ROS and TBARS production and in membrane permeability. By chemical analysis of lemon extracts, two fractions (A and B) that responded to the irradiation were detected, showing a decrease in Fraction A and an increase in Fraction B. Fraction B showed higher antioxidant and antifungal activities in comparison to Fraction A. Both fractions were complex samples enriched in flavonoids. The specific composition of each fraction could explain the difference in the biological activities, and also the higher efficiency of UVBLE to inhibit in vitro the pathogen compared to CLE. Based on our results, UV-B radiation treatment can intensify natural defenses of flavedo lemon by eliciting phenolic compounds synthesis, among other processes.
Fil: Ruiz, Verónica Eugenia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Instituto Superior de Investigaciones Biológicas. Universidad Nacional de Tucumán. Instituto Superior de Investigaciones Biológicas; Argentina
Fil: Cerioni, Luciana. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Instituto Superior de Investigaciones Biológicas. Universidad Nacional de Tucumán. Instituto Superior de Investigaciones Biológicas; Argentina
Fil: Zampini, Iris Catiana. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Instituto de Química del Noroeste. Universidad Nacional de Tucumán. Facultad de Bioquímica, Química y Farmacia. Instituto de Química del Noroeste; Argentina
Fil: Cuello, Ana Soledad. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Instituto de Química del Noroeste. Universidad Nacional de Tucumán. Facultad de Bioquímica, Química y Farmacia. Instituto de Química del Noroeste; Argentina
Fil: Isla, Maria Ines. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Instituto de Química del Noroeste. Universidad Nacional de Tucumán. Facultad de Bioquímica, Química y Farmacia. Instituto de Química del Noroeste; Argentina
Fil: Hilal, Mirna Beatriz. Universidad Nacional de Tucumán. Facultad de Ciencias Naturales e Instituto Miguel Lillo. Cátedra de Fisiología Vegetal; Argentina
Fil: Rapisarda, Viviana Andrea. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Instituto Superior de Investigaciones Biológicas. Universidad Nacional de Tucumán. Instituto Superior de Investigaciones Biológicas; Argentina
Materia
Antimicrobial Activity
Flavonoids
Green Mold
Lemon
Uv-B Radiation
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/65411
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/65411
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling UV-B radiation on lemons enhances antifungal activity of flavedo extracts against Penicillium digitatumRuiz, Verónica EugeniaCerioni, LucianaZampini, Iris CatianaCuello, Ana SoledadIsla, Maria InesHilal, Mirna BeatrizRapisarda, Viviana AndreaAntimicrobial ActivityFlavonoidsGreen MoldLemonUv-B Radiationhttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1UV-B radiation (UVBR) treatment, previously standardized in our laboratory, contributes to prevent green mold caused by Penicillium digitatum on postharvest lemons. Here, antifungal activity of flavedo extracts from irradiated and non-irradiated lemons was assayed against P. digitatum; also, compounds that could be responsible for this action were searched. The flavedo extracts from UV-B exposed lemons (UVBLE) exhibited a higher antimicrobial activity than extracts from control lemons (CLE). Conidia treated with UVBLE showed a time dependent inhibition of germination and oxygen consumption, a markedly increase in ROS and TBARS production and in membrane permeability. By chemical analysis of lemon extracts, two fractions (A and B) that responded to the irradiation were detected, showing a decrease in Fraction A and an increase in Fraction B. Fraction B showed higher antioxidant and antifungal activities in comparison to Fraction A. Both fractions were complex samples enriched in flavonoids. The specific composition of each fraction could explain the difference in the biological activities, and also the higher efficiency of UVBLE to inhibit in vitro the pathogen compared to CLE. Based on our results, UV-B radiation treatment can intensify natural defenses of flavedo lemon by eliciting phenolic compounds synthesis, among other processes.Fil: Ruiz, Verónica Eugenia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Instituto Superior de Investigaciones Biológicas. Universidad Nacional de Tucumán. Instituto Superior de Investigaciones Biológicas; ArgentinaFil: Cerioni, Luciana. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Instituto Superior de Investigaciones Biológicas. Universidad Nacional de Tucumán. Instituto Superior de Investigaciones Biológicas; ArgentinaFil: Zampini, Iris Catiana. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Instituto de Química del Noroeste. Universidad Nacional de Tucumán. Facultad de Bioquímica, Química y Farmacia. Instituto de Química del Noroeste; ArgentinaFil: Cuello, Ana Soledad. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Instituto de Química del Noroeste. Universidad Nacional de Tucumán. Facultad de Bioquímica, Química y Farmacia. Instituto de Química del Noroeste; ArgentinaFil: Isla, Maria Ines. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Instituto de Química del Noroeste. Universidad Nacional de Tucumán. Facultad de Bioquímica, Química y Farmacia. Instituto de Química del Noroeste; ArgentinaFil: Hilal, Mirna Beatriz. Universidad Nacional de Tucumán. Facultad de Ciencias Naturales e Instituto Miguel Lillo. Cátedra de Fisiología Vegetal; ArgentinaFil: Rapisarda, Viviana Andrea. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Instituto Superior de Investigaciones Biológicas. Universidad Nacional de Tucumán. Instituto Superior de Investigaciones Biológicas; ArgentinaElsevier Science2017-11info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfapplication/pdfapplication/pdfapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/65411Ruiz, Verónica Eugenia; Cerioni, Luciana; Zampini, Iris Catiana; Cuello, Ana Soledad; Isla, Maria Ines; et al.; UV-B radiation on lemons enhances antifungal activity of flavedo extracts against Penicillium digitatum; Elsevier Science; LWT - Food Science and Technology; 85; 11-2017; 96-1030023-6438CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/http://linkinghub.elsevier.com/retrieve/pii/S0023643817304711info:eu-repo/semantics/altIdentifier/doi/10.1016/j.lwt.2017.07.002info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-nd/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T10:12:47Zoai:ri.conicet.gov.ar:11336/65411instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 10:12:47.366CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv UV-B radiation on lemons enhances antifungal activity of flavedo extracts against Penicillium digitatum
title UV-B radiation on lemons enhances antifungal activity of flavedo extracts against Penicillium digitatum
spellingShingle UV-B radiation on lemons enhances antifungal activity of flavedo extracts against Penicillium digitatum
Ruiz, Verónica Eugenia
Antimicrobial Activity
Flavonoids
Green Mold
Lemon
Uv-B Radiation
title_short UV-B radiation on lemons enhances antifungal activity of flavedo extracts against Penicillium digitatum
title_full UV-B radiation on lemons enhances antifungal activity of flavedo extracts against Penicillium digitatum
title_fullStr UV-B radiation on lemons enhances antifungal activity of flavedo extracts against Penicillium digitatum
title_full_unstemmed UV-B radiation on lemons enhances antifungal activity of flavedo extracts against Penicillium digitatum
title_sort UV-B radiation on lemons enhances antifungal activity of flavedo extracts against Penicillium digitatum
dc.creator.none.fl_str_mv Ruiz, Verónica Eugenia
Cerioni, Luciana
Zampini, Iris Catiana
Cuello, Ana Soledad
Isla, Maria Ines
Hilal, Mirna Beatriz
Rapisarda, Viviana Andrea
author Ruiz, Verónica Eugenia
author_facet Ruiz, Verónica Eugenia
Cerioni, Luciana
Zampini, Iris Catiana
Cuello, Ana Soledad
Isla, Maria Ines
Hilal, Mirna Beatriz
Rapisarda, Viviana Andrea
author_role author
author2 Cerioni, Luciana
Zampini, Iris Catiana
Cuello, Ana Soledad
Isla, Maria Ines
Hilal, Mirna Beatriz
Rapisarda, Viviana Andrea
author2_role author
author
author
author
author
author
dc.subject.none.fl_str_mv Antimicrobial Activity
Flavonoids
Green Mold
Lemon
Uv-B Radiation
topic Antimicrobial Activity
Flavonoids
Green Mold
Lemon
Uv-B Radiation
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv UV-B radiation (UVBR) treatment, previously standardized in our laboratory, contributes to prevent green mold caused by Penicillium digitatum on postharvest lemons. Here, antifungal activity of flavedo extracts from irradiated and non-irradiated lemons was assayed against P. digitatum; also, compounds that could be responsible for this action were searched. The flavedo extracts from UV-B exposed lemons (UVBLE) exhibited a higher antimicrobial activity than extracts from control lemons (CLE). Conidia treated with UVBLE showed a time dependent inhibition of germination and oxygen consumption, a markedly increase in ROS and TBARS production and in membrane permeability. By chemical analysis of lemon extracts, two fractions (A and B) that responded to the irradiation were detected, showing a decrease in Fraction A and an increase in Fraction B. Fraction B showed higher antioxidant and antifungal activities in comparison to Fraction A. Both fractions were complex samples enriched in flavonoids. The specific composition of each fraction could explain the difference in the biological activities, and also the higher efficiency of UVBLE to inhibit in vitro the pathogen compared to CLE. Based on our results, UV-B radiation treatment can intensify natural defenses of flavedo lemon by eliciting phenolic compounds synthesis, among other processes.
Fil: Ruiz, Verónica Eugenia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Instituto Superior de Investigaciones Biológicas. Universidad Nacional de Tucumán. Instituto Superior de Investigaciones Biológicas; Argentina
Fil: Cerioni, Luciana. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Instituto Superior de Investigaciones Biológicas. Universidad Nacional de Tucumán. Instituto Superior de Investigaciones Biológicas; Argentina
Fil: Zampini, Iris Catiana. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Instituto de Química del Noroeste. Universidad Nacional de Tucumán. Facultad de Bioquímica, Química y Farmacia. Instituto de Química del Noroeste; Argentina
Fil: Cuello, Ana Soledad. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Instituto de Química del Noroeste. Universidad Nacional de Tucumán. Facultad de Bioquímica, Química y Farmacia. Instituto de Química del Noroeste; Argentina
Fil: Isla, Maria Ines. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Instituto de Química del Noroeste. Universidad Nacional de Tucumán. Facultad de Bioquímica, Química y Farmacia. Instituto de Química del Noroeste; Argentina
Fil: Hilal, Mirna Beatriz. Universidad Nacional de Tucumán. Facultad de Ciencias Naturales e Instituto Miguel Lillo. Cátedra de Fisiología Vegetal; Argentina
Fil: Rapisarda, Viviana Andrea. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Instituto Superior de Investigaciones Biológicas. Universidad Nacional de Tucumán. Instituto Superior de Investigaciones Biológicas; Argentina
description UV-B radiation (UVBR) treatment, previously standardized in our laboratory, contributes to prevent green mold caused by Penicillium digitatum on postharvest lemons. Here, antifungal activity of flavedo extracts from irradiated and non-irradiated lemons was assayed against P. digitatum; also, compounds that could be responsible for this action were searched. The flavedo extracts from UV-B exposed lemons (UVBLE) exhibited a higher antimicrobial activity than extracts from control lemons (CLE). Conidia treated with UVBLE showed a time dependent inhibition of germination and oxygen consumption, a markedly increase in ROS and TBARS production and in membrane permeability. By chemical analysis of lemon extracts, two fractions (A and B) that responded to the irradiation were detected, showing a decrease in Fraction A and an increase in Fraction B. Fraction B showed higher antioxidant and antifungal activities in comparison to Fraction A. Both fractions were complex samples enriched in flavonoids. The specific composition of each fraction could explain the difference in the biological activities, and also the higher efficiency of UVBLE to inhibit in vitro the pathogen compared to CLE. Based on our results, UV-B radiation treatment can intensify natural defenses of flavedo lemon by eliciting phenolic compounds synthesis, among other processes.
publishDate 2017
dc.date.none.fl_str_mv 2017-11
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/65411
Ruiz, Verónica Eugenia; Cerioni, Luciana; Zampini, Iris Catiana; Cuello, Ana Soledad; Isla, Maria Ines; et al.; UV-B radiation on lemons enhances antifungal activity of flavedo extracts against Penicillium digitatum; Elsevier Science; LWT - Food Science and Technology; 85; 11-2017; 96-103
0023-6438
CONICET Digital
CONICET
url http://hdl.handle.net/11336/65411
identifier_str_mv Ruiz, Verónica Eugenia; Cerioni, Luciana; Zampini, Iris Catiana; Cuello, Ana Soledad; Isla, Maria Ines; et al.; UV-B radiation on lemons enhances antifungal activity of flavedo extracts against Penicillium digitatum; Elsevier Science; LWT - Food Science and Technology; 85; 11-2017; 96-103
0023-6438
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/http://linkinghub.elsevier.com/retrieve/pii/S0023643817304711
info:eu-repo/semantics/altIdentifier/doi/10.1016/j.lwt.2017.07.002
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
application/pdf
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application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Elsevier Science
publisher.none.fl_str_mv Elsevier Science
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
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instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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