Targeting of flavodoxin to chloroplasts of mesophyll but not bundle sheath maize cells confers increased drought tolerance

Autores
Demarchi, Mariana; Arce, Rocio Cecilia; Campi, Mabel; Pierella Karlusich, Juan José; Hajirezaei, Mohammad Reza; Melzer, Michael; Lodeyro, Anabella Fernanda; Chan, Raquel Lia; Carrillo, Nestor Jose
Año de publicación
2023
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Photosynthetic metabolism in C4 plants is split between two types of dimorphic cells and chloroplasts, with mesophyll chloroplasts performing linear electron transport from water to NADP+, similar to that of C3 plants, and bundle sheath plastids, which have little grana and photosystem II, performing cyclic electron transport and CO2 assimilation. While the negative effects of environmental stresses on C3 photosynthesis are extensively documented, information regarding the stress effects on C4 photosynthetic pathways is scarce and somehow contradictory. We propose in this article a strategy to improve stress tolerance in the main C4 crop, maize, with a minimum of genetic intervention. It is based on the tissue-specific expression of a cyanobacterial flavodoxin, an alternative electron shuttle which prevents over-reduction of the photosynthetic electron transport chain (PETC) under stress, and has been shown to increase stress tolerance in C3 crop and model species. By interacting with the PETC, flavodoxin could be used as a tool to identify which type of C4 photosynthetic pathway is more sensitive to environmental and oxidative challenges.Main questions addressed are therefore: i) which type of genetic modification(s) can provide increased drought and oxidative stress tolerance, and ii) which type of photosynthetic pathway, cell, chloroplast, etc., is more sensitive to environmental hardships in C4 plants.The article reports that flavodoxin does improve stress tolerance in maize, but only when expressed in mesophyll plastids, suggesting that lineal electron transport is the most stress-sensitive pathway, and the one that profits more from flavodoxin interaction. The results reported here open novel avenues of crop improvement in the face of harsher conditions and global climate change affecting sub-irrigated cultivation lands. In addition to its biotech relevance, the results illustrate the use of flavodoxin as a tool to further investigate the response and sensitivity of the different C4 photosynthetic pathways to environmental inputs, a largely unexplored field.
Fil: Demarchi, Mariana. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Rosario. Instituto de Biología Molecular y Celular de Rosario. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Instituto de Biología Molecular y Celular de Rosario; Argentina
Fil: Arce, Rocio Cecilia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Rosario. Instituto de Biología Molecular y Celular de Rosario. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Instituto de Biología Molecular y Celular de Rosario; Argentina
Fil: Campi, Mabel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Instituto de Agrobiotecnología del Litoral. Universidad Nacional del Litoral. Instituto de Agrobiotecnología del Litoral; Argentina
Fil: Pierella Karlusich, Juan José. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Rosario. Instituto de Biología Molecular y Celular de Rosario. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Instituto de Biología Molecular y Celular de Rosario; Argentina
Fil: Hajirezaei, Mohammad Reza. Leibniz Institute Of Plant Genetics And Crop Plant Research.; Alemania
Fil: Melzer, Michael. Leibniz Institute Of Plant Genetics And Crop Plant Research.; Alemania
Fil: Lodeyro, Anabella Fernanda. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Rosario. Instituto de Biología Molecular y Celular de Rosario. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Instituto de Biología Molecular y Celular de Rosario; Argentina
Fil: Chan, Raquel Lia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Instituto de Agrobiotecnología del Litoral. Universidad Nacional del Litoral. Instituto de Agrobiotecnología del Litoral; Argentina
Fil: Carrillo, Nestor Jose. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Rosario. Instituto de Biología Molecular y Celular de Rosario. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Instituto de Biología Molecular y Celular de Rosario; Argentina
Materia
BUNDLE SHEATH CELLS
C4 PLANT
CHLOROPLAST
DROUGHT
FLAVODOXIN
MAIZE
MESOPHYLL CELLS
PHOTOSYNTHESIS
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/225742
Acceder
id CONICETDig_6369118b6075d2c0cae69406a63aa3dc
oai_identifier_str oai:ri.conicet.gov.ar:11336/225742
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Targeting of flavodoxin to chloroplasts of mesophyll but not bundle sheath maize cells confers increased drought toleranceDemarchi, MarianaArce, Rocio CeciliaCampi, MabelPierella Karlusich, Juan JoséHajirezaei, Mohammad RezaMelzer, MichaelLodeyro, Anabella FernandaChan, Raquel LiaCarrillo, Nestor JoseBUNDLE SHEATH CELLSC4 PLANTCHLOROPLASTDROUGHTFLAVODOXINMAIZEMESOPHYLL CELLSPHOTOSYNTHESIShttps://purl.org/becyt/ford/4.4https://purl.org/becyt/ford/4Photosynthetic metabolism in C4 plants is split between two types of dimorphic cells and chloroplasts, with mesophyll chloroplasts performing linear electron transport from water to NADP+, similar to that of C3 plants, and bundle sheath plastids, which have little grana and photosystem II, performing cyclic electron transport and CO2 assimilation. While the negative effects of environmental stresses on C3 photosynthesis are extensively documented, information regarding the stress effects on C4 photosynthetic pathways is scarce and somehow contradictory. We propose in this article a strategy to improve stress tolerance in the main C4 crop, maize, with a minimum of genetic intervention. It is based on the tissue-specific expression of a cyanobacterial flavodoxin, an alternative electron shuttle which prevents over-reduction of the photosynthetic electron transport chain (PETC) under stress, and has been shown to increase stress tolerance in C3 crop and model species. By interacting with the PETC, flavodoxin could be used as a tool to identify which type of C4 photosynthetic pathway is more sensitive to environmental and oxidative challenges.Main questions addressed are therefore: i) which type of genetic modification(s) can provide increased drought and oxidative stress tolerance, and ii) which type of photosynthetic pathway, cell, chloroplast, etc., is more sensitive to environmental hardships in C4 plants.The article reports that flavodoxin does improve stress tolerance in maize, but only when expressed in mesophyll plastids, suggesting that lineal electron transport is the most stress-sensitive pathway, and the one that profits more from flavodoxin interaction. The results reported here open novel avenues of crop improvement in the face of harsher conditions and global climate change affecting sub-irrigated cultivation lands. In addition to its biotech relevance, the results illustrate the use of flavodoxin as a tool to further investigate the response and sensitivity of the different C4 photosynthetic pathways to environmental inputs, a largely unexplored field.Fil: Demarchi, Mariana. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Rosario. Instituto de Biología Molecular y Celular de Rosario. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Instituto de Biología Molecular y Celular de Rosario; ArgentinaFil: Arce, Rocio Cecilia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Rosario. Instituto de Biología Molecular y Celular de Rosario. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Instituto de Biología Molecular y Celular de Rosario; ArgentinaFil: Campi, Mabel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Instituto de Agrobiotecnología del Litoral. Universidad Nacional del Litoral. Instituto de Agrobiotecnología del Litoral; ArgentinaFil: Pierella Karlusich, Juan José. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Rosario. Instituto de Biología Molecular y Celular de Rosario. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Instituto de Biología Molecular y Celular de Rosario; ArgentinaFil: Hajirezaei, Mohammad Reza. Leibniz Institute Of Plant Genetics And Crop Plant Research.; AlemaniaFil: Melzer, Michael. Leibniz Institute Of Plant Genetics And Crop Plant Research.; AlemaniaFil: Lodeyro, Anabella Fernanda. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Rosario. Instituto de Biología Molecular y Celular de Rosario. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Instituto de Biología Molecular y Celular de Rosario; ArgentinaFil: Chan, Raquel Lia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Instituto de Agrobiotecnología del Litoral. Universidad Nacional del Litoral. Instituto de Agrobiotecnología del Litoral; ArgentinaFil: Carrillo, Nestor Jose. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Rosario. Instituto de Biología Molecular y Celular de Rosario. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Instituto de Biología Molecular y Celular de Rosario; ArgentinaWiley Blackwell Publishing, Inc2023-10info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/225742Demarchi, Mariana; Arce, Rocio Cecilia; Campi, Mabel; Pierella Karlusich, Juan José; Hajirezaei, Mohammad Reza; et al.; Targeting of flavodoxin to chloroplasts of mesophyll but not bundle sheath maize cells confers increased drought tolerance; Wiley Blackwell Publishing, Inc; New Phytologist; 240; 6; 10-2023; 2179-21840028-646XCONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://nph.onlinelibrary.wiley.com/doi/10.1111/nph.19281info:eu-repo/semantics/altIdentifier/doi/10.1111/nph.19281info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-03T09:59:39Zoai:ri.conicet.gov.ar:11336/225742instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-03 09:59:39.455CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Targeting of flavodoxin to chloroplasts of mesophyll but not bundle sheath maize cells confers increased drought tolerance
title Targeting of flavodoxin to chloroplasts of mesophyll but not bundle sheath maize cells confers increased drought tolerance
spellingShingle Targeting of flavodoxin to chloroplasts of mesophyll but not bundle sheath maize cells confers increased drought tolerance
Demarchi, Mariana
BUNDLE SHEATH CELLS
C4 PLANT
CHLOROPLAST
DROUGHT
FLAVODOXIN
MAIZE
MESOPHYLL CELLS
PHOTOSYNTHESIS
title_short Targeting of flavodoxin to chloroplasts of mesophyll but not bundle sheath maize cells confers increased drought tolerance
title_full Targeting of flavodoxin to chloroplasts of mesophyll but not bundle sheath maize cells confers increased drought tolerance
title_fullStr Targeting of flavodoxin to chloroplasts of mesophyll but not bundle sheath maize cells confers increased drought tolerance
title_full_unstemmed Targeting of flavodoxin to chloroplasts of mesophyll but not bundle sheath maize cells confers increased drought tolerance
title_sort Targeting of flavodoxin to chloroplasts of mesophyll but not bundle sheath maize cells confers increased drought tolerance
dc.creator.none.fl_str_mv Demarchi, Mariana
Arce, Rocio Cecilia
Campi, Mabel
Pierella Karlusich, Juan José
Hajirezaei, Mohammad Reza
Melzer, Michael
Lodeyro, Anabella Fernanda
Chan, Raquel Lia
Carrillo, Nestor Jose
author Demarchi, Mariana
author_facet Demarchi, Mariana
Arce, Rocio Cecilia
Campi, Mabel
Pierella Karlusich, Juan José
Hajirezaei, Mohammad Reza
Melzer, Michael
Lodeyro, Anabella Fernanda
Chan, Raquel Lia
Carrillo, Nestor Jose
author_role author
author2 Arce, Rocio Cecilia
Campi, Mabel
Pierella Karlusich, Juan José
Hajirezaei, Mohammad Reza
Melzer, Michael
Lodeyro, Anabella Fernanda
Chan, Raquel Lia
Carrillo, Nestor Jose
author2_role author
author
author
author
author
author
author
author
dc.subject.none.fl_str_mv BUNDLE SHEATH CELLS
C4 PLANT
CHLOROPLAST
DROUGHT
FLAVODOXIN
MAIZE
MESOPHYLL CELLS
PHOTOSYNTHESIS
topic BUNDLE SHEATH CELLS
C4 PLANT
CHLOROPLAST
DROUGHT
FLAVODOXIN
MAIZE
MESOPHYLL CELLS
PHOTOSYNTHESIS
purl_subject.fl_str_mv https://purl.org/becyt/ford/4.4
https://purl.org/becyt/ford/4
dc.description.none.fl_txt_mv Photosynthetic metabolism in C4 plants is split between two types of dimorphic cells and chloroplasts, with mesophyll chloroplasts performing linear electron transport from water to NADP+, similar to that of C3 plants, and bundle sheath plastids, which have little grana and photosystem II, performing cyclic electron transport and CO2 assimilation. While the negative effects of environmental stresses on C3 photosynthesis are extensively documented, information regarding the stress effects on C4 photosynthetic pathways is scarce and somehow contradictory. We propose in this article a strategy to improve stress tolerance in the main C4 crop, maize, with a minimum of genetic intervention. It is based on the tissue-specific expression of a cyanobacterial flavodoxin, an alternative electron shuttle which prevents over-reduction of the photosynthetic electron transport chain (PETC) under stress, and has been shown to increase stress tolerance in C3 crop and model species. By interacting with the PETC, flavodoxin could be used as a tool to identify which type of C4 photosynthetic pathway is more sensitive to environmental and oxidative challenges.Main questions addressed are therefore: i) which type of genetic modification(s) can provide increased drought and oxidative stress tolerance, and ii) which type of photosynthetic pathway, cell, chloroplast, etc., is more sensitive to environmental hardships in C4 plants.The article reports that flavodoxin does improve stress tolerance in maize, but only when expressed in mesophyll plastids, suggesting that lineal electron transport is the most stress-sensitive pathway, and the one that profits more from flavodoxin interaction. The results reported here open novel avenues of crop improvement in the face of harsher conditions and global climate change affecting sub-irrigated cultivation lands. In addition to its biotech relevance, the results illustrate the use of flavodoxin as a tool to further investigate the response and sensitivity of the different C4 photosynthetic pathways to environmental inputs, a largely unexplored field.
Fil: Demarchi, Mariana. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Rosario. Instituto de Biología Molecular y Celular de Rosario. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Instituto de Biología Molecular y Celular de Rosario; Argentina
Fil: Arce, Rocio Cecilia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Rosario. Instituto de Biología Molecular y Celular de Rosario. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Instituto de Biología Molecular y Celular de Rosario; Argentina
Fil: Campi, Mabel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Instituto de Agrobiotecnología del Litoral. Universidad Nacional del Litoral. Instituto de Agrobiotecnología del Litoral; Argentina
Fil: Pierella Karlusich, Juan José. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Rosario. Instituto de Biología Molecular y Celular de Rosario. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Instituto de Biología Molecular y Celular de Rosario; Argentina
Fil: Hajirezaei, Mohammad Reza. Leibniz Institute Of Plant Genetics And Crop Plant Research.; Alemania
Fil: Melzer, Michael. Leibniz Institute Of Plant Genetics And Crop Plant Research.; Alemania
Fil: Lodeyro, Anabella Fernanda. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Rosario. Instituto de Biología Molecular y Celular de Rosario. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Instituto de Biología Molecular y Celular de Rosario; Argentina
Fil: Chan, Raquel Lia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Instituto de Agrobiotecnología del Litoral. Universidad Nacional del Litoral. Instituto de Agrobiotecnología del Litoral; Argentina
Fil: Carrillo, Nestor Jose. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Rosario. Instituto de Biología Molecular y Celular de Rosario. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Instituto de Biología Molecular y Celular de Rosario; Argentina
description Photosynthetic metabolism in C4 plants is split between two types of dimorphic cells and chloroplasts, with mesophyll chloroplasts performing linear electron transport from water to NADP+, similar to that of C3 plants, and bundle sheath plastids, which have little grana and photosystem II, performing cyclic electron transport and CO2 assimilation. While the negative effects of environmental stresses on C3 photosynthesis are extensively documented, information regarding the stress effects on C4 photosynthetic pathways is scarce and somehow contradictory. We propose in this article a strategy to improve stress tolerance in the main C4 crop, maize, with a minimum of genetic intervention. It is based on the tissue-specific expression of a cyanobacterial flavodoxin, an alternative electron shuttle which prevents over-reduction of the photosynthetic electron transport chain (PETC) under stress, and has been shown to increase stress tolerance in C3 crop and model species. By interacting with the PETC, flavodoxin could be used as a tool to identify which type of C4 photosynthetic pathway is more sensitive to environmental and oxidative challenges.Main questions addressed are therefore: i) which type of genetic modification(s) can provide increased drought and oxidative stress tolerance, and ii) which type of photosynthetic pathway, cell, chloroplast, etc., is more sensitive to environmental hardships in C4 plants.The article reports that flavodoxin does improve stress tolerance in maize, but only when expressed in mesophyll plastids, suggesting that lineal electron transport is the most stress-sensitive pathway, and the one that profits more from flavodoxin interaction. The results reported here open novel avenues of crop improvement in the face of harsher conditions and global climate change affecting sub-irrigated cultivation lands. In addition to its biotech relevance, the results illustrate the use of flavodoxin as a tool to further investigate the response and sensitivity of the different C4 photosynthetic pathways to environmental inputs, a largely unexplored field.
publishDate 2023
dc.date.none.fl_str_mv 2023-10
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/225742
Demarchi, Mariana; Arce, Rocio Cecilia; Campi, Mabel; Pierella Karlusich, Juan José; Hajirezaei, Mohammad Reza; et al.; Targeting of flavodoxin to chloroplasts of mesophyll but not bundle sheath maize cells confers increased drought tolerance; Wiley Blackwell Publishing, Inc; New Phytologist; 240; 6; 10-2023; 2179-2184
0028-646X
CONICET Digital
CONICET
url http://hdl.handle.net/11336/225742
identifier_str_mv Demarchi, Mariana; Arce, Rocio Cecilia; Campi, Mabel; Pierella Karlusich, Juan José; Hajirezaei, Mohammad Reza; et al.; Targeting of flavodoxin to chloroplasts of mesophyll but not bundle sheath maize cells confers increased drought tolerance; Wiley Blackwell Publishing, Inc; New Phytologist; 240; 6; 10-2023; 2179-2184
0028-646X
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://nph.onlinelibrary.wiley.com/doi/10.1111/nph.19281
info:eu-repo/semantics/altIdentifier/doi/10.1111/nph.19281
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Wiley Blackwell Publishing, Inc
publisher.none.fl_str_mv Wiley Blackwell Publishing, Inc
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.13397

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