Extracellular vesicles secretion: involvement of tne Snarevamp7 and the gtpase Rab39a

Autores
Lujan, Agustin Leonardo; Wojnacki, J.; Rabinovich, Gabriel Adrián; Galli, Team; Damiani, Maria Elena
Año de publicación
2019
Idioma
inglés
Tipo de recurso
documento de conferencia
Estado
versión publicada
Descripción
Secretion of extracellular vesicles (EVs) exerts fundamental roles in almost every tissue, in both physiological and pathological conditions. EVs emerge as important structures involved in cellular communication, particularly in cancer, autoimmunity, infectious diseases, and neurodegenerative disorders. These small membranous formations contain a vast amount of different molecules like nucleic acids, lipids, proteins, and sugars. Hence, EVs could be useful as novel biomarkers and therapies for the treatment of numerous pathologies. Understanding the role played by proteins involved in EVs exocytosis is crucial to control their burden. Rab proteins are the master controllers of vesicular transport. Our laboratory has demonstrated that Rab39a co-localizes with CD63 at multivesicular bodies (MVBs) and regulates the transport of these vesicles. The French laboratory has shown the role of VAMP7 protein in EVs fusion with the plasma membrane. In this collaborative study, we found that Rab39a co-localized with VAMP7 in small vesicles. Furthermore, these proteins modulated EVs secretion in HeLa cells. Interestingly, in VAMP7-knockout cells, the amount of EVs released to the extracellular medium significantly decreased; therefore, EVs cargoes, including molecules with important functions in the immune system, were retained within cells. We found that Galectin-1, a protein of the lectin family, is released through VAMP7-dependent exocytosis. Consequently, impairing VAMP7 function might distort communication and signal transmission among cells, overall affecting immune response.
Fil: Lujan, Agustin Leonardo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Medicina y Biología Experimental de Cuyo; Argentina
Fil: Wojnacki, J.. Inserm; Francia
Fil: Rabinovich, Gabriel Adrián. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biología y Medicina Experimental. Fundación de Instituto de Biología y Medicina Experimental. Instituto de Biología y Medicina Experimental; Argentina
Fil: Galli, Team. Inserm; Francia
Fil: Damiani, Maria Elena. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Medicina y Biología Experimental de Cuyo; Argentina
LV Reunión Anual de la Sociedad Argentina de Investigación en Bioquímica y Biología Molecular; XIV Congreso de la Asociación Panamericana de Bioquímica y Biología Molecular
Salta
Argentina
Sociedad Argentina de Investigación en Bioquímica y Biología Molecular
Asociación Panamericana de Bioquímica y Biología Molecular
Materia
SECRETION
SNARES
RAB PROTEINS
INTRACELLULAR TRANSPORT
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/237056
Acceder
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repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Extracellular vesicles secretion: involvement of tne Snarevamp7 and the gtpase Rab39aLujan, Agustin LeonardoWojnacki, J.Rabinovich, Gabriel AdriánGalli, TeamDamiani, Maria ElenaSECRETIONSNARESRAB PROTEINSINTRACELLULAR TRANSPORThttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1Secretion of extracellular vesicles (EVs) exerts fundamental roles in almost every tissue, in both physiological and pathological conditions. EVs emerge as important structures involved in cellular communication, particularly in cancer, autoimmunity, infectious diseases, and neurodegenerative disorders. These small membranous formations contain a vast amount of different molecules like nucleic acids, lipids, proteins, and sugars. Hence, EVs could be useful as novel biomarkers and therapies for the treatment of numerous pathologies. Understanding the role played by proteins involved in EVs exocytosis is crucial to control their burden. Rab proteins are the master controllers of vesicular transport. Our laboratory has demonstrated that Rab39a co-localizes with CD63 at multivesicular bodies (MVBs) and regulates the transport of these vesicles. The French laboratory has shown the role of VAMP7 protein in EVs fusion with the plasma membrane. In this collaborative study, we found that Rab39a co-localized with VAMP7 in small vesicles. Furthermore, these proteins modulated EVs secretion in HeLa cells. Interestingly, in VAMP7-knockout cells, the amount of EVs released to the extracellular medium significantly decreased; therefore, EVs cargoes, including molecules with important functions in the immune system, were retained within cells. We found that Galectin-1, a protein of the lectin family, is released through VAMP7-dependent exocytosis. Consequently, impairing VAMP7 function might distort communication and signal transmission among cells, overall affecting immune response.Fil: Lujan, Agustin Leonardo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Medicina y Biología Experimental de Cuyo; ArgentinaFil: Wojnacki, J.. Inserm; FranciaFil: Rabinovich, Gabriel Adrián. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biología y Medicina Experimental. Fundación de Instituto de Biología y Medicina Experimental. Instituto de Biología y Medicina Experimental; ArgentinaFil: Galli, Team. Inserm; FranciaFil: Damiani, Maria Elena. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Medicina y Biología Experimental de Cuyo; ArgentinaLV Reunión Anual de la Sociedad Argentina de Investigación en Bioquímica y Biología Molecular; XIV Congreso de la Asociación Panamericana de Bioquímica y Biología MolecularSaltaArgentinaSociedad Argentina de Investigación en Bioquímica y Biología MolecularAsociación Panamericana de Bioquímica y Biología MolecularTech Science Press2019info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/conferenceObjectReuniónJournalhttp://purl.org/coar/resource_type/c_5794info:ar-repo/semantics/documentoDeConferenciaapplication/pdfapplication/vnd.openxmlformats-officedocument.wordprocessingml.documentapplication/pdfhttp://hdl.handle.net/11336/237056Extracellular vesicles secretion: involvement of tne Snarevamp7 and the gtpase Rab39a; LV Reunión Anual de la Sociedad Argentina de Investigación en Bioquímica y Biología Molecular; XIV Congreso de la Asociación Panamericana de Bioquímica y Biología Molecular; Salta; Argentina; 2019; 77-770327-95451667-5746CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://www.saib.org.ar/sites/default/files/BIOCELL-SAIB-2019.pdfNacionalinfo:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-10-15T15:20:54Zoai:ri.conicet.gov.ar:11336/237056instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-10-15 15:20:54.549CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Extracellular vesicles secretion: involvement of tne Snarevamp7 and the gtpase Rab39a
title Extracellular vesicles secretion: involvement of tne Snarevamp7 and the gtpase Rab39a
spellingShingle Extracellular vesicles secretion: involvement of tne Snarevamp7 and the gtpase Rab39a
Lujan, Agustin Leonardo
SECRETION
SNARES
RAB PROTEINS
INTRACELLULAR TRANSPORT
title_short Extracellular vesicles secretion: involvement of tne Snarevamp7 and the gtpase Rab39a
title_full Extracellular vesicles secretion: involvement of tne Snarevamp7 and the gtpase Rab39a
title_fullStr Extracellular vesicles secretion: involvement of tne Snarevamp7 and the gtpase Rab39a
title_full_unstemmed Extracellular vesicles secretion: involvement of tne Snarevamp7 and the gtpase Rab39a
title_sort Extracellular vesicles secretion: involvement of tne Snarevamp7 and the gtpase Rab39a
dc.creator.none.fl_str_mv Lujan, Agustin Leonardo
Wojnacki, J.
Rabinovich, Gabriel Adrián
Galli, Team
Damiani, Maria Elena
author Lujan, Agustin Leonardo
author_facet Lujan, Agustin Leonardo
Wojnacki, J.
Rabinovich, Gabriel Adrián
Galli, Team
Damiani, Maria Elena
author_role author
author2 Wojnacki, J.
Rabinovich, Gabriel Adrián
Galli, Team
Damiani, Maria Elena
author2_role author
author
author
author
dc.subject.none.fl_str_mv SECRETION
SNARES
RAB PROTEINS
INTRACELLULAR TRANSPORT
topic SECRETION
SNARES
RAB PROTEINS
INTRACELLULAR TRANSPORT
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv Secretion of extracellular vesicles (EVs) exerts fundamental roles in almost every tissue, in both physiological and pathological conditions. EVs emerge as important structures involved in cellular communication, particularly in cancer, autoimmunity, infectious diseases, and neurodegenerative disorders. These small membranous formations contain a vast amount of different molecules like nucleic acids, lipids, proteins, and sugars. Hence, EVs could be useful as novel biomarkers and therapies for the treatment of numerous pathologies. Understanding the role played by proteins involved in EVs exocytosis is crucial to control their burden. Rab proteins are the master controllers of vesicular transport. Our laboratory has demonstrated that Rab39a co-localizes with CD63 at multivesicular bodies (MVBs) and regulates the transport of these vesicles. The French laboratory has shown the role of VAMP7 protein in EVs fusion with the plasma membrane. In this collaborative study, we found that Rab39a co-localized with VAMP7 in small vesicles. Furthermore, these proteins modulated EVs secretion in HeLa cells. Interestingly, in VAMP7-knockout cells, the amount of EVs released to the extracellular medium significantly decreased; therefore, EVs cargoes, including molecules with important functions in the immune system, were retained within cells. We found that Galectin-1, a protein of the lectin family, is released through VAMP7-dependent exocytosis. Consequently, impairing VAMP7 function might distort communication and signal transmission among cells, overall affecting immune response.
Fil: Lujan, Agustin Leonardo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Medicina y Biología Experimental de Cuyo; Argentina
Fil: Wojnacki, J.. Inserm; Francia
Fil: Rabinovich, Gabriel Adrián. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biología y Medicina Experimental. Fundación de Instituto de Biología y Medicina Experimental. Instituto de Biología y Medicina Experimental; Argentina
Fil: Galli, Team. Inserm; Francia
Fil: Damiani, Maria Elena. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Medicina y Biología Experimental de Cuyo; Argentina
LV Reunión Anual de la Sociedad Argentina de Investigación en Bioquímica y Biología Molecular; XIV Congreso de la Asociación Panamericana de Bioquímica y Biología Molecular
Salta
Argentina
Sociedad Argentina de Investigación en Bioquímica y Biología Molecular
Asociación Panamericana de Bioquímica y Biología Molecular
description Secretion of extracellular vesicles (EVs) exerts fundamental roles in almost every tissue, in both physiological and pathological conditions. EVs emerge as important structures involved in cellular communication, particularly in cancer, autoimmunity, infectious diseases, and neurodegenerative disorders. These small membranous formations contain a vast amount of different molecules like nucleic acids, lipids, proteins, and sugars. Hence, EVs could be useful as novel biomarkers and therapies for the treatment of numerous pathologies. Understanding the role played by proteins involved in EVs exocytosis is crucial to control their burden. Rab proteins are the master controllers of vesicular transport. Our laboratory has demonstrated that Rab39a co-localizes with CD63 at multivesicular bodies (MVBs) and regulates the transport of these vesicles. The French laboratory has shown the role of VAMP7 protein in EVs fusion with the plasma membrane. In this collaborative study, we found that Rab39a co-localized with VAMP7 in small vesicles. Furthermore, these proteins modulated EVs secretion in HeLa cells. Interestingly, in VAMP7-knockout cells, the amount of EVs released to the extracellular medium significantly decreased; therefore, EVs cargoes, including molecules with important functions in the immune system, were retained within cells. We found that Galectin-1, a protein of the lectin family, is released through VAMP7-dependent exocytosis. Consequently, impairing VAMP7 function might distort communication and signal transmission among cells, overall affecting immune response.
publishDate 2019
dc.date.none.fl_str_mv 2019
dc.type.none.fl_str_mv info:eu-repo/semantics/publishedVersion
info:eu-repo/semantics/conferenceObject
Reunión
Journal
http://purl.org/coar/resource_type/c_5794
info:ar-repo/semantics/documentoDeConferencia
status_str publishedVersion
format conferenceObject
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/237056
Extracellular vesicles secretion: involvement of tne Snarevamp7 and the gtpase Rab39a; LV Reunión Anual de la Sociedad Argentina de Investigación en Bioquímica y Biología Molecular; XIV Congreso de la Asociación Panamericana de Bioquímica y Biología Molecular; Salta; Argentina; 2019; 77-77
0327-9545
1667-5746
CONICET Digital
CONICET
url http://hdl.handle.net/11336/237056
identifier_str_mv Extracellular vesicles secretion: involvement of tne Snarevamp7 and the gtpase Rab39a; LV Reunión Anual de la Sociedad Argentina de Investigación en Bioquímica y Biología Molecular; XIV Congreso de la Asociación Panamericana de Bioquímica y Biología Molecular; Salta; Argentina; 2019; 77-77
0327-9545
1667-5746
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://www.saib.org.ar/sites/default/files/BIOCELL-SAIB-2019.pdf
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/vnd.openxmlformats-officedocument.wordprocessingml.document
application/pdf
dc.coverage.none.fl_str_mv Nacional
dc.publisher.none.fl_str_mv Tech Science Press
publisher.none.fl_str_mv Tech Science Press
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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