Extracellular vesicles secretion: involvement of tne Snarevamp7 and the gtpase Rab39a
- Autores
- Lujan, Agustin Leonardo; Wojnacki, J.; Rabinovich, Gabriel Adrián; Galli, Team; Damiani, Maria Elena
- Año de publicación
- 2019
- Idioma
- inglés
- Tipo de recurso
- documento de conferencia
- Estado
- versión publicada
- Descripción
- Secretion of extracellular vesicles (EVs) exerts fundamental roles in almost every tissue, in both physiological and pathological conditions. EVs emerge as important structures involved in cellular communication, particularly in cancer, autoimmunity, infectious diseases, and neurodegenerative disorders. These small membranous formations contain a vast amount of different molecules like nucleic acids, lipids, proteins, and sugars. Hence, EVs could be useful as novel biomarkers and therapies for the treatment of numerous pathologies. Understanding the role played by proteins involved in EVs exocytosis is crucial to control their burden. Rab proteins are the master controllers of vesicular transport. Our laboratory has demonstrated that Rab39a co-localizes with CD63 at multivesicular bodies (MVBs) and regulates the transport of these vesicles. The French laboratory has shown the role of VAMP7 protein in EVs fusion with the plasma membrane. In this collaborative study, we found that Rab39a co-localized with VAMP7 in small vesicles. Furthermore, these proteins modulated EVs secretion in HeLa cells. Interestingly, in VAMP7-knockout cells, the amount of EVs released to the extracellular medium significantly decreased; therefore, EVs cargoes, including molecules with important functions in the immune system, were retained within cells. We found that Galectin-1, a protein of the lectin family, is released through VAMP7-dependent exocytosis. Consequently, impairing VAMP7 function might distort communication and signal transmission among cells, overall affecting immune response.
Fil: Lujan, Agustin Leonardo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Medicina y Biología Experimental de Cuyo; Argentina
Fil: Wojnacki, J.. Inserm; Francia
Fil: Rabinovich, Gabriel Adrián. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biología y Medicina Experimental. Fundación de Instituto de Biología y Medicina Experimental. Instituto de Biología y Medicina Experimental; Argentina
Fil: Galli, Team. Inserm; Francia
Fil: Damiani, Maria Elena. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Medicina y Biología Experimental de Cuyo; Argentina
LV Reunión Anual de la Sociedad Argentina de Investigación en Bioquímica y Biología Molecular; XIV Congreso de la Asociación Panamericana de Bioquímica y Biología Molecular
Salta
Argentina
Sociedad Argentina de Investigación en Bioquímica y Biología Molecular
Asociación Panamericana de Bioquímica y Biología Molecular - Materia
-
SECRETION
SNARES
RAB PROTEINS
INTRACELLULAR TRANSPORT - Nivel de accesibilidad
- acceso abierto
- Condiciones de uso
- https://creativecommons.org/licenses/by/2.5/ar/
- Repositorio
- Institución
- Consejo Nacional de Investigaciones Científicas y Técnicas
- OAI Identificador
- oai:ri.conicet.gov.ar:11336/237056
Ver los metadatos del registro completo
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Extracellular vesicles secretion: involvement of tne Snarevamp7 and the gtpase Rab39aLujan, Agustin LeonardoWojnacki, J.Rabinovich, Gabriel AdriánGalli, TeamDamiani, Maria ElenaSECRETIONSNARESRAB PROTEINSINTRACELLULAR TRANSPORThttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1Secretion of extracellular vesicles (EVs) exerts fundamental roles in almost every tissue, in both physiological and pathological conditions. EVs emerge as important structures involved in cellular communication, particularly in cancer, autoimmunity, infectious diseases, and neurodegenerative disorders. These small membranous formations contain a vast amount of different molecules like nucleic acids, lipids, proteins, and sugars. Hence, EVs could be useful as novel biomarkers and therapies for the treatment of numerous pathologies. Understanding the role played by proteins involved in EVs exocytosis is crucial to control their burden. Rab proteins are the master controllers of vesicular transport. Our laboratory has demonstrated that Rab39a co-localizes with CD63 at multivesicular bodies (MVBs) and regulates the transport of these vesicles. The French laboratory has shown the role of VAMP7 protein in EVs fusion with the plasma membrane. In this collaborative study, we found that Rab39a co-localized with VAMP7 in small vesicles. Furthermore, these proteins modulated EVs secretion in HeLa cells. Interestingly, in VAMP7-knockout cells, the amount of EVs released to the extracellular medium significantly decreased; therefore, EVs cargoes, including molecules with important functions in the immune system, were retained within cells. We found that Galectin-1, a protein of the lectin family, is released through VAMP7-dependent exocytosis. Consequently, impairing VAMP7 function might distort communication and signal transmission among cells, overall affecting immune response.Fil: Lujan, Agustin Leonardo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Medicina y Biología Experimental de Cuyo; ArgentinaFil: Wojnacki, J.. Inserm; FranciaFil: Rabinovich, Gabriel Adrián. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biología y Medicina Experimental. Fundación de Instituto de Biología y Medicina Experimental. Instituto de Biología y Medicina Experimental; ArgentinaFil: Galli, Team. Inserm; FranciaFil: Damiani, Maria Elena. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Medicina y Biología Experimental de Cuyo; ArgentinaLV Reunión Anual de la Sociedad Argentina de Investigación en Bioquímica y Biología Molecular; XIV Congreso de la Asociación Panamericana de Bioquímica y Biología MolecularSaltaArgentinaSociedad Argentina de Investigación en Bioquímica y Biología MolecularAsociación Panamericana de Bioquímica y Biología MolecularTech Science Press2019info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/conferenceObjectReuniónJournalhttp://purl.org/coar/resource_type/c_5794info:ar-repo/semantics/documentoDeConferenciaapplication/pdfapplication/vnd.openxmlformats-officedocument.wordprocessingml.documentapplication/pdfhttp://hdl.handle.net/11336/237056Extracellular vesicles secretion: involvement of tne Snarevamp7 and the gtpase Rab39a; LV Reunión Anual de la Sociedad Argentina de Investigación en Bioquímica y Biología Molecular; XIV Congreso de la Asociación Panamericana de Bioquímica y Biología Molecular; Salta; Argentina; 2019; 77-770327-95451667-5746CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://www.saib.org.ar/sites/default/files/BIOCELL-SAIB-2019.pdfNacionalinfo:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-10-15T15:20:54Zoai:ri.conicet.gov.ar:11336/237056instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-10-15 15:20:54.549CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse |
dc.title.none.fl_str_mv |
Extracellular vesicles secretion: involvement of tne Snarevamp7 and the gtpase Rab39a |
title |
Extracellular vesicles secretion: involvement of tne Snarevamp7 and the gtpase Rab39a |
spellingShingle |
Extracellular vesicles secretion: involvement of tne Snarevamp7 and the gtpase Rab39a Lujan, Agustin Leonardo SECRETION SNARES RAB PROTEINS INTRACELLULAR TRANSPORT |
title_short |
Extracellular vesicles secretion: involvement of tne Snarevamp7 and the gtpase Rab39a |
title_full |
Extracellular vesicles secretion: involvement of tne Snarevamp7 and the gtpase Rab39a |
title_fullStr |
Extracellular vesicles secretion: involvement of tne Snarevamp7 and the gtpase Rab39a |
title_full_unstemmed |
Extracellular vesicles secretion: involvement of tne Snarevamp7 and the gtpase Rab39a |
title_sort |
Extracellular vesicles secretion: involvement of tne Snarevamp7 and the gtpase Rab39a |
dc.creator.none.fl_str_mv |
Lujan, Agustin Leonardo Wojnacki, J. Rabinovich, Gabriel Adrián Galli, Team Damiani, Maria Elena |
author |
Lujan, Agustin Leonardo |
author_facet |
Lujan, Agustin Leonardo Wojnacki, J. Rabinovich, Gabriel Adrián Galli, Team Damiani, Maria Elena |
author_role |
author |
author2 |
Wojnacki, J. Rabinovich, Gabriel Adrián Galli, Team Damiani, Maria Elena |
author2_role |
author author author author |
dc.subject.none.fl_str_mv |
SECRETION SNARES RAB PROTEINS INTRACELLULAR TRANSPORT |
topic |
SECRETION SNARES RAB PROTEINS INTRACELLULAR TRANSPORT |
purl_subject.fl_str_mv |
https://purl.org/becyt/ford/1.6 https://purl.org/becyt/ford/1 |
dc.description.none.fl_txt_mv |
Secretion of extracellular vesicles (EVs) exerts fundamental roles in almost every tissue, in both physiological and pathological conditions. EVs emerge as important structures involved in cellular communication, particularly in cancer, autoimmunity, infectious diseases, and neurodegenerative disorders. These small membranous formations contain a vast amount of different molecules like nucleic acids, lipids, proteins, and sugars. Hence, EVs could be useful as novel biomarkers and therapies for the treatment of numerous pathologies. Understanding the role played by proteins involved in EVs exocytosis is crucial to control their burden. Rab proteins are the master controllers of vesicular transport. Our laboratory has demonstrated that Rab39a co-localizes with CD63 at multivesicular bodies (MVBs) and regulates the transport of these vesicles. The French laboratory has shown the role of VAMP7 protein in EVs fusion with the plasma membrane. In this collaborative study, we found that Rab39a co-localized with VAMP7 in small vesicles. Furthermore, these proteins modulated EVs secretion in HeLa cells. Interestingly, in VAMP7-knockout cells, the amount of EVs released to the extracellular medium significantly decreased; therefore, EVs cargoes, including molecules with important functions in the immune system, were retained within cells. We found that Galectin-1, a protein of the lectin family, is released through VAMP7-dependent exocytosis. Consequently, impairing VAMP7 function might distort communication and signal transmission among cells, overall affecting immune response. Fil: Lujan, Agustin Leonardo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Medicina y Biología Experimental de Cuyo; Argentina Fil: Wojnacki, J.. Inserm; Francia Fil: Rabinovich, Gabriel Adrián. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biología y Medicina Experimental. Fundación de Instituto de Biología y Medicina Experimental. Instituto de Biología y Medicina Experimental; Argentina Fil: Galli, Team. Inserm; Francia Fil: Damiani, Maria Elena. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Medicina y Biología Experimental de Cuyo; Argentina LV Reunión Anual de la Sociedad Argentina de Investigación en Bioquímica y Biología Molecular; XIV Congreso de la Asociación Panamericana de Bioquímica y Biología Molecular Salta Argentina Sociedad Argentina de Investigación en Bioquímica y Biología Molecular Asociación Panamericana de Bioquímica y Biología Molecular |
description |
Secretion of extracellular vesicles (EVs) exerts fundamental roles in almost every tissue, in both physiological and pathological conditions. EVs emerge as important structures involved in cellular communication, particularly in cancer, autoimmunity, infectious diseases, and neurodegenerative disorders. These small membranous formations contain a vast amount of different molecules like nucleic acids, lipids, proteins, and sugars. Hence, EVs could be useful as novel biomarkers and therapies for the treatment of numerous pathologies. Understanding the role played by proteins involved in EVs exocytosis is crucial to control their burden. Rab proteins are the master controllers of vesicular transport. Our laboratory has demonstrated that Rab39a co-localizes with CD63 at multivesicular bodies (MVBs) and regulates the transport of these vesicles. The French laboratory has shown the role of VAMP7 protein in EVs fusion with the plasma membrane. In this collaborative study, we found that Rab39a co-localized with VAMP7 in small vesicles. Furthermore, these proteins modulated EVs secretion in HeLa cells. Interestingly, in VAMP7-knockout cells, the amount of EVs released to the extracellular medium significantly decreased; therefore, EVs cargoes, including molecules with important functions in the immune system, were retained within cells. We found that Galectin-1, a protein of the lectin family, is released through VAMP7-dependent exocytosis. Consequently, impairing VAMP7 function might distort communication and signal transmission among cells, overall affecting immune response. |
publishDate |
2019 |
dc.date.none.fl_str_mv |
2019 |
dc.type.none.fl_str_mv |
info:eu-repo/semantics/publishedVersion info:eu-repo/semantics/conferenceObject Reunión Journal http://purl.org/coar/resource_type/c_5794 info:ar-repo/semantics/documentoDeConferencia |
status_str |
publishedVersion |
format |
conferenceObject |
dc.identifier.none.fl_str_mv |
http://hdl.handle.net/11336/237056 Extracellular vesicles secretion: involvement of tne Snarevamp7 and the gtpase Rab39a; LV Reunión Anual de la Sociedad Argentina de Investigación en Bioquímica y Biología Molecular; XIV Congreso de la Asociación Panamericana de Bioquímica y Biología Molecular; Salta; Argentina; 2019; 77-77 0327-9545 1667-5746 CONICET Digital CONICET |
url |
http://hdl.handle.net/11336/237056 |
identifier_str_mv |
Extracellular vesicles secretion: involvement of tne Snarevamp7 and the gtpase Rab39a; LV Reunión Anual de la Sociedad Argentina de Investigación en Bioquímica y Biología Molecular; XIV Congreso de la Asociación Panamericana de Bioquímica y Biología Molecular; Salta; Argentina; 2019; 77-77 0327-9545 1667-5746 CONICET Digital CONICET |
dc.language.none.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
info:eu-repo/semantics/altIdentifier/url/https://www.saib.org.ar/sites/default/files/BIOCELL-SAIB-2019.pdf |
dc.rights.none.fl_str_mv |
info:eu-repo/semantics/openAccess https://creativecommons.org/licenses/by/2.5/ar/ |
eu_rights_str_mv |
openAccess |
rights_invalid_str_mv |
https://creativecommons.org/licenses/by/2.5/ar/ |
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application/pdf application/vnd.openxmlformats-officedocument.wordprocessingml.document application/pdf |
dc.coverage.none.fl_str_mv |
Nacional |
dc.publisher.none.fl_str_mv |
Tech Science Press |
publisher.none.fl_str_mv |
Tech Science Press |
dc.source.none.fl_str_mv |
reponame:CONICET Digital (CONICET) instname:Consejo Nacional de Investigaciones Científicas y Técnicas |
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Consejo Nacional de Investigaciones Científicas y Técnicas |
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CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas |
repository.mail.fl_str_mv |
dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar |
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13.22299 |