The late endocytic Rab39a GTPase regulates the interaction between multivesicular bodies and chlamydial inclusions.

Autores
Gambarte Tudela, Julian Alberto; Capmany, Anahi; Romao, Maryse; Quintero, Cristian Andres; Miserey Lenkei, Stephanie; Raposo, Graça; Goud, Bruno; Damiani, Maria Elena
Año de publicación
2015
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Given their obligate intracellular lifestyle, Chlamydia trachomatis ensure their access to multiple host sources of essential lipids by interfering vesicular transport. These bacteria hijack Rab6-, Rab11- and Rab14-controlled trafficking pathways to acquire sphingomyelin from the Golgi apparatus. Another important source of sphingolipids, phospholipids and cholesterol are multivesicular bodies (MVBs). Despite their participation in chlamydial inclusion development and bacterial replication, the molecular mechanisms mediating MVBs-inclusion interaction remain unknown. In the present study, we demonstrate that Rab39a labels a subset of late endocytic vesicles -mainly MVBs- that move along microtubules. Moreover, Rab39a is actively recruited to chlamydial inclusions throughout the pathogen life cycle by a bacterial-driven process and depending on its GTP/GDP binding state. Interestingly, Rab39a participates in the delivery of MVB and host sphingolipids to maturing chlamydial inclusions thereby promoting inclusion growth and bacterial development. Altogether, our findings indicate that Rab39a favours chlamydial replication and infectivity. This is the first report showing a late endocytic Rab GTPase involved in chlamydial infection development.
Fil: Gambarte Tudela, Julian Alberto. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Histología y Embriología de Mendoza Dr. Mario H. Burgos. Universidad Nacional de Cuyo. Facultad de Cienicas Médicas. Instituto de Histología y Embriología de Mendoza Dr. Mario H. Burgos; Argentina
Fil: Capmany, Anahi. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Histología y Embriología de Mendoza Dr. Mario H. Burgos. Universidad Nacional de Cuyo. Facultad de Cienicas Médicas. Instituto de Histología y Embriología de Mendoza Dr. Mario H. Burgos; Argentina
Fil: Romao, Maryse. Institute Curie; Francia
Fil: Quintero, Cristian Andres. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Histología y Embriología de Mendoza Dr. Mario H. Burgos. Universidad Nacional de Cuyo. Facultad de Cienicas Médicas. Instituto de Histología y Embriología de Mendoza Dr. Mario H. Burgos; Argentina
Fil: Miserey Lenkei, Stephanie. Institute Curie; Francia
Fil: Raposo, Graça. Institute Curie; Francia
Fil: Goud, Bruno. Institute Curie; Francia
Fil: Damiani, Maria Elena. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Histología y Embriología de Mendoza Dr. Mario H. Burgos. Universidad Nacional de Cuyo. Facultad de Cienicas Médicas. Instituto de Histología y Embriología de Mendoza Dr. Mario H. Burgos; Argentina
Materia
CHLAMYDIA TRACHOMATIS
RAB PROTEINS
MULTIVESICULAR BODIES
PATHOGEN-HOST CELL INTERACTION
IPID TRANSPORT
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/41303
Acceder
id CONICETDig_f5df6c4e27ea924e0134d5f04ed2af4b
oai_identifier_str oai:ri.conicet.gov.ar:11336/41303
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling The late endocytic Rab39a GTPase regulates the interaction between multivesicular bodies and chlamydial inclusions.Gambarte Tudela, Julian AlbertoCapmany, AnahiRomao, MaryseQuintero, Cristian AndresMiserey Lenkei, StephanieRaposo, GraçaGoud, BrunoDamiani, Maria ElenaCHLAMYDIA TRACHOMATISRAB PROTEINSMULTIVESICULAR BODIESPATHOGEN-HOST CELL INTERACTIONIPID TRANSPORThttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1Given their obligate intracellular lifestyle, Chlamydia trachomatis ensure their access to multiple host sources of essential lipids by interfering vesicular transport. These bacteria hijack Rab6-, Rab11- and Rab14-controlled trafficking pathways to acquire sphingomyelin from the Golgi apparatus. Another important source of sphingolipids, phospholipids and cholesterol are multivesicular bodies (MVBs). Despite their participation in chlamydial inclusion development and bacterial replication, the molecular mechanisms mediating MVBs-inclusion interaction remain unknown. In the present study, we demonstrate that Rab39a labels a subset of late endocytic vesicles -mainly MVBs- that move along microtubules. Moreover, Rab39a is actively recruited to chlamydial inclusions throughout the pathogen life cycle by a bacterial-driven process and depending on its GTP/GDP binding state. Interestingly, Rab39a participates in the delivery of MVB and host sphingolipids to maturing chlamydial inclusions thereby promoting inclusion growth and bacterial development. Altogether, our findings indicate that Rab39a favours chlamydial replication and infectivity. This is the first report showing a late endocytic Rab GTPase involved in chlamydial infection development.Fil: Gambarte Tudela, Julian Alberto. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Histología y Embriología de Mendoza Dr. Mario H. Burgos. Universidad Nacional de Cuyo. Facultad de Cienicas Médicas. Instituto de Histología y Embriología de Mendoza Dr. Mario H. Burgos; ArgentinaFil: Capmany, Anahi. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Histología y Embriología de Mendoza Dr. Mario H. Burgos. Universidad Nacional de Cuyo. Facultad de Cienicas Médicas. Instituto de Histología y Embriología de Mendoza Dr. Mario H. Burgos; ArgentinaFil: Romao, Maryse. Institute Curie; FranciaFil: Quintero, Cristian Andres. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Histología y Embriología de Mendoza Dr. Mario H. Burgos. Universidad Nacional de Cuyo. Facultad de Cienicas Médicas. Instituto de Histología y Embriología de Mendoza Dr. Mario H. Burgos; ArgentinaFil: Miserey Lenkei, Stephanie. Institute Curie; FranciaFil: Raposo, Graça. Institute Curie; FranciaFil: Goud, Bruno. Institute Curie; FranciaFil: Damiani, Maria Elena. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Histología y Embriología de Mendoza Dr. Mario H. Burgos. Universidad Nacional de Cuyo. Facultad de Cienicas Médicas. Instituto de Histología y Embriología de Mendoza Dr. Mario H. Burgos; ArgentinaCompany of Biologists2015-08info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/41303Gambarte Tudela, Julian Alberto; Capmany, Anahi; Romao, Maryse; Quintero, Cristian Andres; Miserey Lenkei, Stephanie; et al.; The late endocytic Rab39a GTPase regulates the interaction between multivesicular bodies and chlamydial inclusions.; Company of Biologists; Journal of Cell Science; 128; 16; 8-2015; 3068-30810021-95331477-9137CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.1242/jcs.170092info:eu-repo/semantics/altIdentifier/url/http://jcs.biologists.org/content/128/16/3068info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T09:34:55Zoai:ri.conicet.gov.ar:11336/41303instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 09:34:56.202CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv The late endocytic Rab39a GTPase regulates the interaction between multivesicular bodies and chlamydial inclusions.
title The late endocytic Rab39a GTPase regulates the interaction between multivesicular bodies and chlamydial inclusions.
spellingShingle The late endocytic Rab39a GTPase regulates the interaction between multivesicular bodies and chlamydial inclusions.
Gambarte Tudela, Julian Alberto
CHLAMYDIA TRACHOMATIS
RAB PROTEINS
MULTIVESICULAR BODIES
PATHOGEN-HOST CELL INTERACTION
IPID TRANSPORT
title_short The late endocytic Rab39a GTPase regulates the interaction between multivesicular bodies and chlamydial inclusions.
title_full The late endocytic Rab39a GTPase regulates the interaction between multivesicular bodies and chlamydial inclusions.
title_fullStr The late endocytic Rab39a GTPase regulates the interaction between multivesicular bodies and chlamydial inclusions.
title_full_unstemmed The late endocytic Rab39a GTPase regulates the interaction between multivesicular bodies and chlamydial inclusions.
title_sort The late endocytic Rab39a GTPase regulates the interaction between multivesicular bodies and chlamydial inclusions.
dc.creator.none.fl_str_mv Gambarte Tudela, Julian Alberto
Capmany, Anahi
Romao, Maryse
Quintero, Cristian Andres
Miserey Lenkei, Stephanie
Raposo, Graça
Goud, Bruno
Damiani, Maria Elena
author Gambarte Tudela, Julian Alberto
author_facet Gambarte Tudela, Julian Alberto
Capmany, Anahi
Romao, Maryse
Quintero, Cristian Andres
Miserey Lenkei, Stephanie
Raposo, Graça
Goud, Bruno
Damiani, Maria Elena
author_role author
author2 Capmany, Anahi
Romao, Maryse
Quintero, Cristian Andres
Miserey Lenkei, Stephanie
Raposo, Graça
Goud, Bruno
Damiani, Maria Elena
author2_role author
author
author
author
author
author
author
dc.subject.none.fl_str_mv CHLAMYDIA TRACHOMATIS
RAB PROTEINS
MULTIVESICULAR BODIES
PATHOGEN-HOST CELL INTERACTION
IPID TRANSPORT
topic CHLAMYDIA TRACHOMATIS
RAB PROTEINS
MULTIVESICULAR BODIES
PATHOGEN-HOST CELL INTERACTION
IPID TRANSPORT
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv Given their obligate intracellular lifestyle, Chlamydia trachomatis ensure their access to multiple host sources of essential lipids by interfering vesicular transport. These bacteria hijack Rab6-, Rab11- and Rab14-controlled trafficking pathways to acquire sphingomyelin from the Golgi apparatus. Another important source of sphingolipids, phospholipids and cholesterol are multivesicular bodies (MVBs). Despite their participation in chlamydial inclusion development and bacterial replication, the molecular mechanisms mediating MVBs-inclusion interaction remain unknown. In the present study, we demonstrate that Rab39a labels a subset of late endocytic vesicles -mainly MVBs- that move along microtubules. Moreover, Rab39a is actively recruited to chlamydial inclusions throughout the pathogen life cycle by a bacterial-driven process and depending on its GTP/GDP binding state. Interestingly, Rab39a participates in the delivery of MVB and host sphingolipids to maturing chlamydial inclusions thereby promoting inclusion growth and bacterial development. Altogether, our findings indicate that Rab39a favours chlamydial replication and infectivity. This is the first report showing a late endocytic Rab GTPase involved in chlamydial infection development.
Fil: Gambarte Tudela, Julian Alberto. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Histología y Embriología de Mendoza Dr. Mario H. Burgos. Universidad Nacional de Cuyo. Facultad de Cienicas Médicas. Instituto de Histología y Embriología de Mendoza Dr. Mario H. Burgos; Argentina
Fil: Capmany, Anahi. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Histología y Embriología de Mendoza Dr. Mario H. Burgos. Universidad Nacional de Cuyo. Facultad de Cienicas Médicas. Instituto de Histología y Embriología de Mendoza Dr. Mario H. Burgos; Argentina
Fil: Romao, Maryse. Institute Curie; Francia
Fil: Quintero, Cristian Andres. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Histología y Embriología de Mendoza Dr. Mario H. Burgos. Universidad Nacional de Cuyo. Facultad de Cienicas Médicas. Instituto de Histología y Embriología de Mendoza Dr. Mario H. Burgos; Argentina
Fil: Miserey Lenkei, Stephanie. Institute Curie; Francia
Fil: Raposo, Graça. Institute Curie; Francia
Fil: Goud, Bruno. Institute Curie; Francia
Fil: Damiani, Maria Elena. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Histología y Embriología de Mendoza Dr. Mario H. Burgos. Universidad Nacional de Cuyo. Facultad de Cienicas Médicas. Instituto de Histología y Embriología de Mendoza Dr. Mario H. Burgos; Argentina
description Given their obligate intracellular lifestyle, Chlamydia trachomatis ensure their access to multiple host sources of essential lipids by interfering vesicular transport. These bacteria hijack Rab6-, Rab11- and Rab14-controlled trafficking pathways to acquire sphingomyelin from the Golgi apparatus. Another important source of sphingolipids, phospholipids and cholesterol are multivesicular bodies (MVBs). Despite their participation in chlamydial inclusion development and bacterial replication, the molecular mechanisms mediating MVBs-inclusion interaction remain unknown. In the present study, we demonstrate that Rab39a labels a subset of late endocytic vesicles -mainly MVBs- that move along microtubules. Moreover, Rab39a is actively recruited to chlamydial inclusions throughout the pathogen life cycle by a bacterial-driven process and depending on its GTP/GDP binding state. Interestingly, Rab39a participates in the delivery of MVB and host sphingolipids to maturing chlamydial inclusions thereby promoting inclusion growth and bacterial development. Altogether, our findings indicate that Rab39a favours chlamydial replication and infectivity. This is the first report showing a late endocytic Rab GTPase involved in chlamydial infection development.
publishDate 2015
dc.date.none.fl_str_mv 2015-08
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/41303
Gambarte Tudela, Julian Alberto; Capmany, Anahi; Romao, Maryse; Quintero, Cristian Andres; Miserey Lenkei, Stephanie; et al.; The late endocytic Rab39a GTPase regulates the interaction between multivesicular bodies and chlamydial inclusions.; Company of Biologists; Journal of Cell Science; 128; 16; 8-2015; 3068-3081
0021-9533
1477-9137
CONICET Digital
CONICET
url http://hdl.handle.net/11336/41303
identifier_str_mv Gambarte Tudela, Julian Alberto; Capmany, Anahi; Romao, Maryse; Quintero, Cristian Andres; Miserey Lenkei, Stephanie; et al.; The late endocytic Rab39a GTPase regulates the interaction between multivesicular bodies and chlamydial inclusions.; Company of Biologists; Journal of Cell Science; 128; 16; 8-2015; 3068-3081
0021-9533
1477-9137
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.1242/jcs.170092
info:eu-repo/semantics/altIdentifier/url/http://jcs.biologists.org/content/128/16/3068
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Company of Biologists
publisher.none.fl_str_mv Company of Biologists
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.070432

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