Screening of lactic acid bacteria isolates from dairy and cereal products for exopolysaccharide production and genes involved

Autores
Van der Meulen, Roel; Grosu Tudor, Silvia; Mozzi, Fernanda Beatriz; Vaningelgem, Frederik; Zamfir, Medana; Font, Graciela Maria; De Vuyst, Luc
Año de publicación
2007
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
A total of 174 lactic acid bacteria (LAB) strains isolated from dairy and cereal products were screened for the production of exopolysaccharides (EPS). Therefore, a rapid screening method was developed based on ultrafiltration and gel permeation chromatography. Furthermore, a screening through the polymerase chain reaction (PCR) was performed with primer pairs targeting different genes involved in EPS production. Nine isolates produced a homopolysaccharide of the glucan type, whereas only one strain produced a heteropolysaccharide. The production of a glucan by a strain of Lactococcus lactis and the production of a heteropolysaccharide by a strain of Lactobacillus curvatus are reported for the first time. The PCR screening revealed many positive strains. For three of the ten EPS-producing strains, no corresponding genes could be detected. Furthermore, a lot of strains possessed one or more eps genes but did not produce an EPS. Therefore, a screening on the molecular level should always be accompanied by another screening method that is able to distinguish true EPS producer strains from non-producing ones. Statistical analysis did not reveal any relationship between the type and origin of the strains, the presence or absence of a capsular polysaccharide or EPS, and the presence or absence of eps genes.
Fil: Van der Meulen, Roel. Vrije Universiteit Brussel; Bélgica
Fil: Grosu Tudor, Silvia. Vrije Universiteit Brussel; Bélgica
Fil: Mozzi, Fernanda Beatriz. Vrije Universiteit Brussel; Bélgica. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Centro de Referencia para Lactobacilos; Argentina
Fil: Vaningelgem, Frederik. Vrije Universiteit Brussel; Bélgica
Fil: Zamfir, Medana. Vrije Universiteit Brussel; Bélgica. Romanian Academy. Cell Biology Department. Institute of Biology; Rumania
Fil: Font, Graciela Maria. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Centro de Referencia para Lactobacilos; Argentina. Universidad Nacional de Tucumán. Facultad de Bioquímica, Química y Farmacia; Argentina
Fil: De Vuyst, Luc. Vrije Universiteit Brussel; Bélgica
Materia
Exopolysaccharides
Lactic Acid Bacteria
Screening
Ultrafiltration
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/56482
Acceder
id CONICETDig_62e732053b2bca2484284e19e092b8b0
oai_identifier_str oai:ri.conicet.gov.ar:11336/56482
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Screening of lactic acid bacteria isolates from dairy and cereal products for exopolysaccharide production and genes involvedVan der Meulen, RoelGrosu Tudor, SilviaMozzi, Fernanda BeatrizVaningelgem, FrederikZamfir, MedanaFont, Graciela MariaDe Vuyst, LucExopolysaccharidesLactic Acid BacteriaScreeningUltrafiltrationhttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1A total of 174 lactic acid bacteria (LAB) strains isolated from dairy and cereal products were screened for the production of exopolysaccharides (EPS). Therefore, a rapid screening method was developed based on ultrafiltration and gel permeation chromatography. Furthermore, a screening through the polymerase chain reaction (PCR) was performed with primer pairs targeting different genes involved in EPS production. Nine isolates produced a homopolysaccharide of the glucan type, whereas only one strain produced a heteropolysaccharide. The production of a glucan by a strain of Lactococcus lactis and the production of a heteropolysaccharide by a strain of Lactobacillus curvatus are reported for the first time. The PCR screening revealed many positive strains. For three of the ten EPS-producing strains, no corresponding genes could be detected. Furthermore, a lot of strains possessed one or more eps genes but did not produce an EPS. Therefore, a screening on the molecular level should always be accompanied by another screening method that is able to distinguish true EPS producer strains from non-producing ones. Statistical analysis did not reveal any relationship between the type and origin of the strains, the presence or absence of a capsular polysaccharide or EPS, and the presence or absence of eps genes.Fil: Van der Meulen, Roel. Vrije Universiteit Brussel; BélgicaFil: Grosu Tudor, Silvia. Vrije Universiteit Brussel; BélgicaFil: Mozzi, Fernanda Beatriz. Vrije Universiteit Brussel; Bélgica. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Centro de Referencia para Lactobacilos; ArgentinaFil: Vaningelgem, Frederik. Vrije Universiteit Brussel; BélgicaFil: Zamfir, Medana. Vrije Universiteit Brussel; Bélgica. Romanian Academy. Cell Biology Department. Institute of Biology; RumaniaFil: Font, Graciela Maria. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Centro de Referencia para Lactobacilos; Argentina. Universidad Nacional de Tucumán. Facultad de Bioquímica, Química y Farmacia; ArgentinaFil: De Vuyst, Luc. Vrije Universiteit Brussel; BélgicaElsevier Science2007-09-30info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/56482Van der Meulen, Roel; Grosu Tudor, Silvia; Mozzi, Fernanda Beatriz; Vaningelgem, Frederik; Zamfir, Medana; et al.; Screening of lactic acid bacteria isolates from dairy and cereal products for exopolysaccharide production and genes involved; Elsevier Science; International Journal of Food Microbiology; 118; 3; 30-9-2007; 250-2580168-16051879-3460CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.1016/j.ijfoodmicro.2007.07.014info:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/pii/S0168160507003601info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-10T13:06:30Zoai:ri.conicet.gov.ar:11336/56482instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-10 13:06:30.838CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Screening of lactic acid bacteria isolates from dairy and cereal products for exopolysaccharide production and genes involved
title Screening of lactic acid bacteria isolates from dairy and cereal products for exopolysaccharide production and genes involved
spellingShingle Screening of lactic acid bacteria isolates from dairy and cereal products for exopolysaccharide production and genes involved
Van der Meulen, Roel
Exopolysaccharides
Lactic Acid Bacteria
Screening
Ultrafiltration
title_short Screening of lactic acid bacteria isolates from dairy and cereal products for exopolysaccharide production and genes involved
title_full Screening of lactic acid bacteria isolates from dairy and cereal products for exopolysaccharide production and genes involved
title_fullStr Screening of lactic acid bacteria isolates from dairy and cereal products for exopolysaccharide production and genes involved
title_full_unstemmed Screening of lactic acid bacteria isolates from dairy and cereal products for exopolysaccharide production and genes involved
title_sort Screening of lactic acid bacteria isolates from dairy and cereal products for exopolysaccharide production and genes involved
dc.creator.none.fl_str_mv Van der Meulen, Roel
Grosu Tudor, Silvia
Mozzi, Fernanda Beatriz
Vaningelgem, Frederik
Zamfir, Medana
Font, Graciela Maria
De Vuyst, Luc
author Van der Meulen, Roel
author_facet Van der Meulen, Roel
Grosu Tudor, Silvia
Mozzi, Fernanda Beatriz
Vaningelgem, Frederik
Zamfir, Medana
Font, Graciela Maria
De Vuyst, Luc
author_role author
author2 Grosu Tudor, Silvia
Mozzi, Fernanda Beatriz
Vaningelgem, Frederik
Zamfir, Medana
Font, Graciela Maria
De Vuyst, Luc
author2_role author
author
author
author
author
author
dc.subject.none.fl_str_mv Exopolysaccharides
Lactic Acid Bacteria
Screening
Ultrafiltration
topic Exopolysaccharides
Lactic Acid Bacteria
Screening
Ultrafiltration
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv A total of 174 lactic acid bacteria (LAB) strains isolated from dairy and cereal products were screened for the production of exopolysaccharides (EPS). Therefore, a rapid screening method was developed based on ultrafiltration and gel permeation chromatography. Furthermore, a screening through the polymerase chain reaction (PCR) was performed with primer pairs targeting different genes involved in EPS production. Nine isolates produced a homopolysaccharide of the glucan type, whereas only one strain produced a heteropolysaccharide. The production of a glucan by a strain of Lactococcus lactis and the production of a heteropolysaccharide by a strain of Lactobacillus curvatus are reported for the first time. The PCR screening revealed many positive strains. For three of the ten EPS-producing strains, no corresponding genes could be detected. Furthermore, a lot of strains possessed one or more eps genes but did not produce an EPS. Therefore, a screening on the molecular level should always be accompanied by another screening method that is able to distinguish true EPS producer strains from non-producing ones. Statistical analysis did not reveal any relationship between the type and origin of the strains, the presence or absence of a capsular polysaccharide or EPS, and the presence or absence of eps genes.
Fil: Van der Meulen, Roel. Vrije Universiteit Brussel; Bélgica
Fil: Grosu Tudor, Silvia. Vrije Universiteit Brussel; Bélgica
Fil: Mozzi, Fernanda Beatriz. Vrije Universiteit Brussel; Bélgica. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Centro de Referencia para Lactobacilos; Argentina
Fil: Vaningelgem, Frederik. Vrije Universiteit Brussel; Bélgica
Fil: Zamfir, Medana. Vrije Universiteit Brussel; Bélgica. Romanian Academy. Cell Biology Department. Institute of Biology; Rumania
Fil: Font, Graciela Maria. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Centro de Referencia para Lactobacilos; Argentina. Universidad Nacional de Tucumán. Facultad de Bioquímica, Química y Farmacia; Argentina
Fil: De Vuyst, Luc. Vrije Universiteit Brussel; Bélgica
description A total of 174 lactic acid bacteria (LAB) strains isolated from dairy and cereal products were screened for the production of exopolysaccharides (EPS). Therefore, a rapid screening method was developed based on ultrafiltration and gel permeation chromatography. Furthermore, a screening through the polymerase chain reaction (PCR) was performed with primer pairs targeting different genes involved in EPS production. Nine isolates produced a homopolysaccharide of the glucan type, whereas only one strain produced a heteropolysaccharide. The production of a glucan by a strain of Lactococcus lactis and the production of a heteropolysaccharide by a strain of Lactobacillus curvatus are reported for the first time. The PCR screening revealed many positive strains. For three of the ten EPS-producing strains, no corresponding genes could be detected. Furthermore, a lot of strains possessed one or more eps genes but did not produce an EPS. Therefore, a screening on the molecular level should always be accompanied by another screening method that is able to distinguish true EPS producer strains from non-producing ones. Statistical analysis did not reveal any relationship between the type and origin of the strains, the presence or absence of a capsular polysaccharide or EPS, and the presence or absence of eps genes.
publishDate 2007
dc.date.none.fl_str_mv 2007-09-30
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/56482
Van der Meulen, Roel; Grosu Tudor, Silvia; Mozzi, Fernanda Beatriz; Vaningelgem, Frederik; Zamfir, Medana; et al.; Screening of lactic acid bacteria isolates from dairy and cereal products for exopolysaccharide production and genes involved; Elsevier Science; International Journal of Food Microbiology; 118; 3; 30-9-2007; 250-258
0168-1605
1879-3460
CONICET Digital
CONICET
url http://hdl.handle.net/11336/56482
identifier_str_mv Van der Meulen, Roel; Grosu Tudor, Silvia; Mozzi, Fernanda Beatriz; Vaningelgem, Frederik; Zamfir, Medana; et al.; Screening of lactic acid bacteria isolates from dairy and cereal products for exopolysaccharide production and genes involved; Elsevier Science; International Journal of Food Microbiology; 118; 3; 30-9-2007; 250-258
0168-1605
1879-3460
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.1016/j.ijfoodmicro.2007.07.014
info:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/pii/S0168160507003601
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Elsevier Science
publisher.none.fl_str_mv Elsevier Science
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
_version_ 1842980270988328960
score 12.993085

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