Functionality of exopolysaccharides produced by lactic acid bacteria in an in vitro gastric system

Autores
Mozzi, Fernanda Beatriz; Gerbino, Oscar Esteban; Font, Graciela Maria; Torino, Maria Ines
Año de publicación
2009
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Aims: To evaluate whether slime-exopolysaccharides (EPS) or capsular-polysaccharide (CPS) production could protect the polymer-producing strains Streptococcus thermophilus CRL 1190 and Lactobacillus casei CRL 87 against the harsh conditions of an in vitro gastric system (GS). EPS stability on the GS was studied. Methods and Results: An in vitro GS model containing human saliva and gastric juice was standardized. Polymer functionality on the cell viability and metabolic activity of the EPS-producing strains in the GS acidic conditions was evaluated. Two isogenic EPS/CPS deficient mutants were used for comparison. EPS or CPS conferred no significant protection on the cell viability of the studied strains after passage through the GS conditions. However, the phospho- and β-galactosidase activities of the EPS+ strains were higher than those of the EPS-. Cytoplasmic alterations in the wild-type and mutant strains and partial degradation of both EPS were detected. Conclusions: The presence of EPS/CPS protected the metabolic activity of the assayed LAB strains, but had no effect on survival at low pH. Significance and Impact of the Study: The presence of EPS/CPS as well as polymer resistance to the harsh conditions of the human GS could impact positively in probiotic strains to exert their properties in the host.
Fil: Mozzi, Fernanda Beatriz. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Centro de Referencia para Lactobacilos; Argentina
Fil: Gerbino, Oscar Esteban. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Centro de Referencia para Lactobacilos; Argentina
Fil: Font, Graciela Maria. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Centro de Referencia para Lactobacilos; Argentina. Universidad Nacional de Tucumán. Facultad de Bioquímica, Química y Farmacia; Argentina
Fil: Torino, Maria Ines. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Centro de Referencia para Lactobacilos; Argentina
Materia
Exopolysaccharides
Gastric System
Lactic Acid Bacteria
Physiological Function
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/54888
Acceder
id CONICETDig_1bbc4e265b514515b26e4ce441680151
oai_identifier_str oai:ri.conicet.gov.ar:11336/54888
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Functionality of exopolysaccharides produced by lactic acid bacteria in an in vitro gastric systemMozzi, Fernanda BeatrizGerbino, Oscar EstebanFont, Graciela MariaTorino, Maria InesExopolysaccharidesGastric SystemLactic Acid BacteriaPhysiological Functionhttps://purl.org/becyt/ford/2.11https://purl.org/becyt/ford/2Aims: To evaluate whether slime-exopolysaccharides (EPS) or capsular-polysaccharide (CPS) production could protect the polymer-producing strains Streptococcus thermophilus CRL 1190 and Lactobacillus casei CRL 87 against the harsh conditions of an in vitro gastric system (GS). EPS stability on the GS was studied. Methods and Results: An in vitro GS model containing human saliva and gastric juice was standardized. Polymer functionality on the cell viability and metabolic activity of the EPS-producing strains in the GS acidic conditions was evaluated. Two isogenic EPS/CPS deficient mutants were used for comparison. EPS or CPS conferred no significant protection on the cell viability of the studied strains after passage through the GS conditions. However, the phospho- and β-galactosidase activities of the EPS+ strains were higher than those of the EPS-. Cytoplasmic alterations in the wild-type and mutant strains and partial degradation of both EPS were detected. Conclusions: The presence of EPS/CPS protected the metabolic activity of the assayed LAB strains, but had no effect on survival at low pH. Significance and Impact of the Study: The presence of EPS/CPS as well as polymer resistance to the harsh conditions of the human GS could impact positively in probiotic strains to exert their properties in the host.Fil: Mozzi, Fernanda Beatriz. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Centro de Referencia para Lactobacilos; ArgentinaFil: Gerbino, Oscar Esteban. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Centro de Referencia para Lactobacilos; ArgentinaFil: Font, Graciela Maria. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Centro de Referencia para Lactobacilos; Argentina. Universidad Nacional de Tucumán. Facultad de Bioquímica, Química y Farmacia; ArgentinaFil: Torino, Maria Ines. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Centro de Referencia para Lactobacilos; ArgentinaWiley Blackwell Publishing, Inc2009-07info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/54888Mozzi, Fernanda Beatriz; Gerbino, Oscar Esteban; Font, Graciela Maria; Torino, Maria Ines; Functionality of exopolysaccharides produced by lactic acid bacteria in an in vitro gastric system; Wiley Blackwell Publishing, Inc; Journal of Applied Microbiology; 107; 1; 7-2009; 56-641364-50721365-2672CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.1111/j.1365-2672.2009.04182.xinfo:eu-repo/semantics/altIdentifier/url/https://onlinelibrary.wiley.com/doi/abs/10.1111/j.1365-2672.2009.04182.xinfo:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-10-15T15:22:19Zoai:ri.conicet.gov.ar:11336/54888instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-10-15 15:22:19.807CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Functionality of exopolysaccharides produced by lactic acid bacteria in an in vitro gastric system
title Functionality of exopolysaccharides produced by lactic acid bacteria in an in vitro gastric system
spellingShingle Functionality of exopolysaccharides produced by lactic acid bacteria in an in vitro gastric system
Mozzi, Fernanda Beatriz
Exopolysaccharides
Gastric System
Lactic Acid Bacteria
Physiological Function
title_short Functionality of exopolysaccharides produced by lactic acid bacteria in an in vitro gastric system
title_full Functionality of exopolysaccharides produced by lactic acid bacteria in an in vitro gastric system
title_fullStr Functionality of exopolysaccharides produced by lactic acid bacteria in an in vitro gastric system
title_full_unstemmed Functionality of exopolysaccharides produced by lactic acid bacteria in an in vitro gastric system
title_sort Functionality of exopolysaccharides produced by lactic acid bacteria in an in vitro gastric system
dc.creator.none.fl_str_mv Mozzi, Fernanda Beatriz
Gerbino, Oscar Esteban
Font, Graciela Maria
Torino, Maria Ines
author Mozzi, Fernanda Beatriz
author_facet Mozzi, Fernanda Beatriz
Gerbino, Oscar Esteban
Font, Graciela Maria
Torino, Maria Ines
author_role author
author2 Gerbino, Oscar Esteban
Font, Graciela Maria
Torino, Maria Ines
author2_role author
author
author
dc.subject.none.fl_str_mv Exopolysaccharides
Gastric System
Lactic Acid Bacteria
Physiological Function
topic Exopolysaccharides
Gastric System
Lactic Acid Bacteria
Physiological Function
purl_subject.fl_str_mv https://purl.org/becyt/ford/2.11
https://purl.org/becyt/ford/2
dc.description.none.fl_txt_mv Aims: To evaluate whether slime-exopolysaccharides (EPS) or capsular-polysaccharide (CPS) production could protect the polymer-producing strains Streptococcus thermophilus CRL 1190 and Lactobacillus casei CRL 87 against the harsh conditions of an in vitro gastric system (GS). EPS stability on the GS was studied. Methods and Results: An in vitro GS model containing human saliva and gastric juice was standardized. Polymer functionality on the cell viability and metabolic activity of the EPS-producing strains in the GS acidic conditions was evaluated. Two isogenic EPS/CPS deficient mutants were used for comparison. EPS or CPS conferred no significant protection on the cell viability of the studied strains after passage through the GS conditions. However, the phospho- and β-galactosidase activities of the EPS+ strains were higher than those of the EPS-. Cytoplasmic alterations in the wild-type and mutant strains and partial degradation of both EPS were detected. Conclusions: The presence of EPS/CPS protected the metabolic activity of the assayed LAB strains, but had no effect on survival at low pH. Significance and Impact of the Study: The presence of EPS/CPS as well as polymer resistance to the harsh conditions of the human GS could impact positively in probiotic strains to exert their properties in the host.
Fil: Mozzi, Fernanda Beatriz. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Centro de Referencia para Lactobacilos; Argentina
Fil: Gerbino, Oscar Esteban. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Centro de Referencia para Lactobacilos; Argentina
Fil: Font, Graciela Maria. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Centro de Referencia para Lactobacilos; Argentina. Universidad Nacional de Tucumán. Facultad de Bioquímica, Química y Farmacia; Argentina
Fil: Torino, Maria Ines. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Centro de Referencia para Lactobacilos; Argentina
description Aims: To evaluate whether slime-exopolysaccharides (EPS) or capsular-polysaccharide (CPS) production could protect the polymer-producing strains Streptococcus thermophilus CRL 1190 and Lactobacillus casei CRL 87 against the harsh conditions of an in vitro gastric system (GS). EPS stability on the GS was studied. Methods and Results: An in vitro GS model containing human saliva and gastric juice was standardized. Polymer functionality on the cell viability and metabolic activity of the EPS-producing strains in the GS acidic conditions was evaluated. Two isogenic EPS/CPS deficient mutants were used for comparison. EPS or CPS conferred no significant protection on the cell viability of the studied strains after passage through the GS conditions. However, the phospho- and β-galactosidase activities of the EPS+ strains were higher than those of the EPS-. Cytoplasmic alterations in the wild-type and mutant strains and partial degradation of both EPS were detected. Conclusions: The presence of EPS/CPS protected the metabolic activity of the assayed LAB strains, but had no effect on survival at low pH. Significance and Impact of the Study: The presence of EPS/CPS as well as polymer resistance to the harsh conditions of the human GS could impact positively in probiotic strains to exert their properties in the host.
publishDate 2009
dc.date.none.fl_str_mv 2009-07
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/54888
Mozzi, Fernanda Beatriz; Gerbino, Oscar Esteban; Font, Graciela Maria; Torino, Maria Ines; Functionality of exopolysaccharides produced by lactic acid bacteria in an in vitro gastric system; Wiley Blackwell Publishing, Inc; Journal of Applied Microbiology; 107; 1; 7-2009; 56-64
1364-5072
1365-2672
CONICET Digital
CONICET
url http://hdl.handle.net/11336/54888
identifier_str_mv Mozzi, Fernanda Beatriz; Gerbino, Oscar Esteban; Font, Graciela Maria; Torino, Maria Ines; Functionality of exopolysaccharides produced by lactic acid bacteria in an in vitro gastric system; Wiley Blackwell Publishing, Inc; Journal of Applied Microbiology; 107; 1; 7-2009; 56-64
1364-5072
1365-2672
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.1111/j.1365-2672.2009.04182.x
info:eu-repo/semantics/altIdentifier/url/https://onlinelibrary.wiley.com/doi/abs/10.1111/j.1365-2672.2009.04182.x
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Wiley Blackwell Publishing, Inc
publisher.none.fl_str_mv Wiley Blackwell Publishing, Inc
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
_version_ 1846083370334814208
score 13.221938

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