Native bacteria isolated from roots and rhizosphere of Solanum lycopersicum L. increase tomato seedling growth under a reduced fertilization regime

Autores
Pérez Rodriguez, María Micaela; Piccoli, Patricia Noemí; Anzuay, María Soledad; Baraldi, Rita; Neri, Luisa; Taurian, Tania; Lobato Ureche, Miguel Andrés; Segura, Diana María; Cohen, Ana Carmen
Año de publicación
2020
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
In semiarid regions is important to use native strains best adapted to these environments to optimize plant-PGPR interaction. We aimed to isolate and characterize PGPR from roots and rhizosphere of a tomato crop, as well as studying the effect of its inoculation on tomato seedlings growth. We selected four strains considering their effectiveness of fixing nitrogen, solubilizing phosphate, producing siderophores and indole acetic acid. They belong to the genera Enterobacter, Pseudomonas, Cellulosimicrobium, and Ochrobactrum. In addition, we also analyzed the ability to solubilize Ca3(PO4)2, FePO4 and AlPO4 and the presence of one of the genes encoding the cofactor PQQ in their genome. Enterobacter 64S1 and Pseudomonas 42P4 showed the highest phosphorus solubilizing activity and presence of pqqE gene. Furthermore, in a tomato-based bioassay in speed-bed demonstrated that a sole inoculation at seedling stage with the strains increased dry weight of roots (49–88%) and shoots (39–55%), stem height (8–13%) and diameter (5–8%) and leaf area (22–31%) and were equal or even higher than fertilization treatment. Leaf nitrogen and chlorophyll levels were also increased (50–80% and 26–33%) compared to control. These results suggest that Enterobacter 64S1 and Pseudomonas 42P4 can be used as bio-inoculant in order to realize a nutrient integrated management.
Fil: Pérez Rodriguez, María Micaela. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Biología Agrícola de Mendoza. Universidad Nacional de Cuyo. Facultad de Ciencias Agrarias. Instituto de Biología Agrícola de Mendoza; Argentina
Fil: Piccoli, Patricia Noemí. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Biología Agrícola de Mendoza. Universidad Nacional de Cuyo. Facultad de Ciencias Agrarias. Instituto de Biología Agrícola de Mendoza; Argentina
Fil: Anzuay, María Soledad. Universidad Nacional de Río Cuarto; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Baraldi, Rita. Consiglio Nazionale delle Ricerche; Italia
Fil: Neri, Luisa. Consiglio Nazionale delle Ricerche; Italia
Fil: Taurian, Tania. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional de Río Cuarto; Argentina
Fil: Lobato Ureche, Miguel Andrés. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Biología Agrícola de Mendoza. Universidad Nacional de Cuyo. Facultad de Ciencias Agrarias. Instituto de Biología Agrícola de Mendoza; Argentina
Fil: Segura, Diana María. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Biología Agrícola de Mendoza. Universidad Nacional de Cuyo. Facultad de Ciencias Agrarias. Instituto de Biología Agrícola de Mendoza; Argentina
Fil: Cohen, Ana Carmen. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Biología Agrícola de Mendoza. Universidad Nacional de Cuyo. Facultad de Ciencias Agrarias. Instituto de Biología Agrícola de Mendoza; Argentina
Materia
TOMATE
PSEUDOMONA
ENTEROBACTER
PGPR
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/128701
Acceder
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network_name_str CONICET Digital (CONICET)
spelling Native bacteria isolated from roots and rhizosphere of Solanum lycopersicum L. increase tomato seedling growth under a reduced fertilization regimePérez Rodriguez, María MicaelaPiccoli, Patricia NoemíAnzuay, María SoledadBaraldi, RitaNeri, LuisaTaurian, TaniaLobato Ureche, Miguel AndrésSegura, Diana MaríaCohen, Ana CarmenTOMATEPSEUDOMONAENTEROBACTERPGPRhttps://purl.org/becyt/ford/4.5https://purl.org/becyt/ford/4In semiarid regions is important to use native strains best adapted to these environments to optimize plant-PGPR interaction. We aimed to isolate and characterize PGPR from roots and rhizosphere of a tomato crop, as well as studying the effect of its inoculation on tomato seedlings growth. We selected four strains considering their effectiveness of fixing nitrogen, solubilizing phosphate, producing siderophores and indole acetic acid. They belong to the genera Enterobacter, Pseudomonas, Cellulosimicrobium, and Ochrobactrum. In addition, we also analyzed the ability to solubilize Ca3(PO4)2, FePO4 and AlPO4 and the presence of one of the genes encoding the cofactor PQQ in their genome. Enterobacter 64S1 and Pseudomonas 42P4 showed the highest phosphorus solubilizing activity and presence of pqqE gene. Furthermore, in a tomato-based bioassay in speed-bed demonstrated that a sole inoculation at seedling stage with the strains increased dry weight of roots (49–88%) and shoots (39–55%), stem height (8–13%) and diameter (5–8%) and leaf area (22–31%) and were equal or even higher than fertilization treatment. Leaf nitrogen and chlorophyll levels were also increased (50–80% and 26–33%) compared to control. These results suggest that Enterobacter 64S1 and Pseudomonas 42P4 can be used as bio-inoculant in order to realize a nutrient integrated management.Fil: Pérez Rodriguez, María Micaela. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Biología Agrícola de Mendoza. Universidad Nacional de Cuyo. Facultad de Ciencias Agrarias. Instituto de Biología Agrícola de Mendoza; ArgentinaFil: Piccoli, Patricia Noemí. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Biología Agrícola de Mendoza. Universidad Nacional de Cuyo. Facultad de Ciencias Agrarias. Instituto de Biología Agrícola de Mendoza; ArgentinaFil: Anzuay, María Soledad. Universidad Nacional de Río Cuarto; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Baraldi, Rita. Consiglio Nazionale delle Ricerche; ItaliaFil: Neri, Luisa. Consiglio Nazionale delle Ricerche; ItaliaFil: Taurian, Tania. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional de Río Cuarto; ArgentinaFil: Lobato Ureche, Miguel Andrés. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Biología Agrícola de Mendoza. Universidad Nacional de Cuyo. Facultad de Ciencias Agrarias. Instituto de Biología Agrícola de Mendoza; ArgentinaFil: Segura, Diana María. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Biología Agrícola de Mendoza. Universidad Nacional de Cuyo. Facultad de Ciencias Agrarias. Instituto de Biología Agrícola de Mendoza; ArgentinaFil: Cohen, Ana Carmen. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Biología Agrícola de Mendoza. Universidad Nacional de Cuyo. Facultad de Ciencias Agrarias. Instituto de Biología Agrícola de Mendoza; ArgentinaNature Publishing Group2020-12info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/128701Pérez Rodriguez, María Micaela; Piccoli, Patricia Noemí; Anzuay, María Soledad; Baraldi, Rita; Neri, Luisa; et al.; Native bacteria isolated from roots and rhizosphere of Solanum lycopersicum L. increase tomato seedling growth under a reduced fertilization regime; Nature Publishing Group; Scientific Reports; 10; 15642; 12-2020; 1-142045-2322CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/http://www.nature.com/articles/s41598-020-72507-4info:eu-repo/semantics/altIdentifier/doi/10.1038/s41598-020-72507-4info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-10T13:25:31Zoai:ri.conicet.gov.ar:11336/128701instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-10 13:25:31.342CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Native bacteria isolated from roots and rhizosphere of Solanum lycopersicum L. increase tomato seedling growth under a reduced fertilization regime
title Native bacteria isolated from roots and rhizosphere of Solanum lycopersicum L. increase tomato seedling growth under a reduced fertilization regime
spellingShingle Native bacteria isolated from roots and rhizosphere of Solanum lycopersicum L. increase tomato seedling growth under a reduced fertilization regime
Pérez Rodriguez, María Micaela
TOMATE
PSEUDOMONA
ENTEROBACTER
PGPR
title_short Native bacteria isolated from roots and rhizosphere of Solanum lycopersicum L. increase tomato seedling growth under a reduced fertilization regime
title_full Native bacteria isolated from roots and rhizosphere of Solanum lycopersicum L. increase tomato seedling growth under a reduced fertilization regime
title_fullStr Native bacteria isolated from roots and rhizosphere of Solanum lycopersicum L. increase tomato seedling growth under a reduced fertilization regime
title_full_unstemmed Native bacteria isolated from roots and rhizosphere of Solanum lycopersicum L. increase tomato seedling growth under a reduced fertilization regime
title_sort Native bacteria isolated from roots and rhizosphere of Solanum lycopersicum L. increase tomato seedling growth under a reduced fertilization regime
dc.creator.none.fl_str_mv Pérez Rodriguez, María Micaela
Piccoli, Patricia Noemí
Anzuay, María Soledad
Baraldi, Rita
Neri, Luisa
Taurian, Tania
Lobato Ureche, Miguel Andrés
Segura, Diana María
Cohen, Ana Carmen
author Pérez Rodriguez, María Micaela
author_facet Pérez Rodriguez, María Micaela
Piccoli, Patricia Noemí
Anzuay, María Soledad
Baraldi, Rita
Neri, Luisa
Taurian, Tania
Lobato Ureche, Miguel Andrés
Segura, Diana María
Cohen, Ana Carmen
author_role author
author2 Piccoli, Patricia Noemí
Anzuay, María Soledad
Baraldi, Rita
Neri, Luisa
Taurian, Tania
Lobato Ureche, Miguel Andrés
Segura, Diana María
Cohen, Ana Carmen
author2_role author
author
author
author
author
author
author
author
dc.subject.none.fl_str_mv TOMATE
PSEUDOMONA
ENTEROBACTER
PGPR
topic TOMATE
PSEUDOMONA
ENTEROBACTER
PGPR
purl_subject.fl_str_mv https://purl.org/becyt/ford/4.5
https://purl.org/becyt/ford/4
dc.description.none.fl_txt_mv In semiarid regions is important to use native strains best adapted to these environments to optimize plant-PGPR interaction. We aimed to isolate and characterize PGPR from roots and rhizosphere of a tomato crop, as well as studying the effect of its inoculation on tomato seedlings growth. We selected four strains considering their effectiveness of fixing nitrogen, solubilizing phosphate, producing siderophores and indole acetic acid. They belong to the genera Enterobacter, Pseudomonas, Cellulosimicrobium, and Ochrobactrum. In addition, we also analyzed the ability to solubilize Ca3(PO4)2, FePO4 and AlPO4 and the presence of one of the genes encoding the cofactor PQQ in their genome. Enterobacter 64S1 and Pseudomonas 42P4 showed the highest phosphorus solubilizing activity and presence of pqqE gene. Furthermore, in a tomato-based bioassay in speed-bed demonstrated that a sole inoculation at seedling stage with the strains increased dry weight of roots (49–88%) and shoots (39–55%), stem height (8–13%) and diameter (5–8%) and leaf area (22–31%) and were equal or even higher than fertilization treatment. Leaf nitrogen and chlorophyll levels were also increased (50–80% and 26–33%) compared to control. These results suggest that Enterobacter 64S1 and Pseudomonas 42P4 can be used as bio-inoculant in order to realize a nutrient integrated management.
Fil: Pérez Rodriguez, María Micaela. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Biología Agrícola de Mendoza. Universidad Nacional de Cuyo. Facultad de Ciencias Agrarias. Instituto de Biología Agrícola de Mendoza; Argentina
Fil: Piccoli, Patricia Noemí. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Biología Agrícola de Mendoza. Universidad Nacional de Cuyo. Facultad de Ciencias Agrarias. Instituto de Biología Agrícola de Mendoza; Argentina
Fil: Anzuay, María Soledad. Universidad Nacional de Río Cuarto; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Baraldi, Rita. Consiglio Nazionale delle Ricerche; Italia
Fil: Neri, Luisa. Consiglio Nazionale delle Ricerche; Italia
Fil: Taurian, Tania. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional de Río Cuarto; Argentina
Fil: Lobato Ureche, Miguel Andrés. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Biología Agrícola de Mendoza. Universidad Nacional de Cuyo. Facultad de Ciencias Agrarias. Instituto de Biología Agrícola de Mendoza; Argentina
Fil: Segura, Diana María. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Biología Agrícola de Mendoza. Universidad Nacional de Cuyo. Facultad de Ciencias Agrarias. Instituto de Biología Agrícola de Mendoza; Argentina
Fil: Cohen, Ana Carmen. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Biología Agrícola de Mendoza. Universidad Nacional de Cuyo. Facultad de Ciencias Agrarias. Instituto de Biología Agrícola de Mendoza; Argentina
description In semiarid regions is important to use native strains best adapted to these environments to optimize plant-PGPR interaction. We aimed to isolate and characterize PGPR from roots and rhizosphere of a tomato crop, as well as studying the effect of its inoculation on tomato seedlings growth. We selected four strains considering their effectiveness of fixing nitrogen, solubilizing phosphate, producing siderophores and indole acetic acid. They belong to the genera Enterobacter, Pseudomonas, Cellulosimicrobium, and Ochrobactrum. In addition, we also analyzed the ability to solubilize Ca3(PO4)2, FePO4 and AlPO4 and the presence of one of the genes encoding the cofactor PQQ in their genome. Enterobacter 64S1 and Pseudomonas 42P4 showed the highest phosphorus solubilizing activity and presence of pqqE gene. Furthermore, in a tomato-based bioassay in speed-bed demonstrated that a sole inoculation at seedling stage with the strains increased dry weight of roots (49–88%) and shoots (39–55%), stem height (8–13%) and diameter (5–8%) and leaf area (22–31%) and were equal or even higher than fertilization treatment. Leaf nitrogen and chlorophyll levels were also increased (50–80% and 26–33%) compared to control. These results suggest that Enterobacter 64S1 and Pseudomonas 42P4 can be used as bio-inoculant in order to realize a nutrient integrated management.
publishDate 2020
dc.date.none.fl_str_mv 2020-12
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/128701
Pérez Rodriguez, María Micaela; Piccoli, Patricia Noemí; Anzuay, María Soledad; Baraldi, Rita; Neri, Luisa; et al.; Native bacteria isolated from roots and rhizosphere of Solanum lycopersicum L. increase tomato seedling growth under a reduced fertilization regime; Nature Publishing Group; Scientific Reports; 10; 15642; 12-2020; 1-14
2045-2322
CONICET Digital
CONICET
url http://hdl.handle.net/11336/128701
identifier_str_mv Pérez Rodriguez, María Micaela; Piccoli, Patricia Noemí; Anzuay, María Soledad; Baraldi, Rita; Neri, Luisa; et al.; Native bacteria isolated from roots and rhizosphere of Solanum lycopersicum L. increase tomato seedling growth under a reduced fertilization regime; Nature Publishing Group; Scientific Reports; 10; 15642; 12-2020; 1-14
2045-2322
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/http://www.nature.com/articles/s41598-020-72507-4
info:eu-repo/semantics/altIdentifier/doi/10.1038/s41598-020-72507-4
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Nature Publishing Group
publisher.none.fl_str_mv Nature Publishing Group
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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