Swine influenza: clinical, serological, pathological, and virological cross-sectional studies in nine farms in Argentina

Autores
Dibárbora, Marina; Cappuccio, Javier Alejandro; Olivera, Valeria; Quiroga, Maria; Machuca, Mariana Alejandra; Perfumo, Carlos Juan; Pérez, Daniel Roberto; Pereda, Ariel Julián
Año de publicación
2013
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Background Influenza A viruses (IAV) are important pathogens responsible for economic losses in the swine industry and represent a threat to public health. In Argentina, clinical, pathological, and virological findings suggest that IAV infection is widespread among pig farms. In addition, several subtypes of IAV, such as pH1N1, H3N2, δ1H1N1, and δ2H1N2, have been reported. Objectives To evaluate the infection patterns of influenza virus in nine pig farms in Argentina. Methods Clinical, serological, pathological, and virological cross-sectional studies were conducted. Results Clinical and pathological results were characteristic of endemic influenza infection in eight of the nine farms studied. By rRT-PCR, six of the nine farms were positive to influenza. Five IAV were obtained. Genome analysis determined that four of the isolations were pH1N1 and that the remaining one was a reassortant human origin H3N2 virus containing pandemic internal genes. Serological results showed that all farms were positive to influenza A antibodies. Moreover, the hemagglutination inhibition test showed that infection with viruses containing HA′s from different subtypes (pH1, δ1H1, δ2H1, and H3) is present among the farms studied and that coinfections with two or more subtypes were present in 80.5% of positive pigs. Conclusions Because vaccines against IAV are not licensed in Argentina, these results reflect the situation of IAV infection in non-vaccinated herds. This study provides more information about the circulation and characteristics of IAV in a poorly surveyed region. This study provides more data that will be used to evaluate the tools necessary to control this disease.
Fil: Dibárbora, Marina. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigación en Ciencias Veterinarias y Agronómicas; Argentina. Instituto Nacional de Tecnología Agropecuaria; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Cappuccio, Javier Alejandro. Universidad Nacional de la Plata. Facultad de Ciencias Veterinarias. Catedra de Patologia Especial; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Olivera, Valeria. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigación en Ciencias Veterinarias y Agronómicas; Argentina. Instituto Nacional de Tecnología Agropecuaria; Argentina
Fil: Quiroga, Maria. Universidad Nacional de la Plata. Facultad de Ciencias Veterinarias. Catedra de Patologia Especial; Argentina
Fil: Machuca, Mariana Alejandra. Universidad Nacional de la Plata. Facultad de Ciencias Veterinarias. Catedra de Patologia Especial; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Perfumo, Carlos Juan. Universidad Nacional de la Plata. Facultad de Ciencias Veterinarias. Catedra de Patologia Especial; Argentina
Fil: Pérez, Daniel Roberto. University of Maryland; Estados Unidos
Fil: Pereda, Ariel Julián. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigación en Ciencias Veterinarias y Agronómicas; Argentina. Instituto Nacional de Tecnología Agropecuaria; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Materia
ARGENTINA
INFLUENZA
PATHOLOGY
SEROLOGY
SWINE
VIROLOGY
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/24278
Acceder
id CONICETDig_62411ee48dedae071fc5789e6936dd99
oai_identifier_str oai:ri.conicet.gov.ar:11336/24278
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Swine influenza: clinical, serological, pathological, and virological cross-sectional studies in nine farms in ArgentinaDibárbora, MarinaCappuccio, Javier AlejandroOlivera, ValeriaQuiroga, MariaMachuca, Mariana AlejandraPerfumo, Carlos JuanPérez, Daniel RobertoPereda, Ariel JuliánARGENTINAINFLUENZAPATHOLOGYSEROLOGYSWINEVIROLOGYBackground Influenza A viruses (IAV) are important pathogens responsible for economic losses in the swine industry and represent a threat to public health. In Argentina, clinical, pathological, and virological findings suggest that IAV infection is widespread among pig farms. In addition, several subtypes of IAV, such as pH1N1, H3N2, δ1H1N1, and δ2H1N2, have been reported. Objectives To evaluate the infection patterns of influenza virus in nine pig farms in Argentina. Methods Clinical, serological, pathological, and virological cross-sectional studies were conducted. Results Clinical and pathological results were characteristic of endemic influenza infection in eight of the nine farms studied. By rRT-PCR, six of the nine farms were positive to influenza. Five IAV were obtained. Genome analysis determined that four of the isolations were pH1N1 and that the remaining one was a reassortant human origin H3N2 virus containing pandemic internal genes. Serological results showed that all farms were positive to influenza A antibodies. Moreover, the hemagglutination inhibition test showed that infection with viruses containing HA′s from different subtypes (pH1, δ1H1, δ2H1, and H3) is present among the farms studied and that coinfections with two or more subtypes were present in 80.5% of positive pigs. Conclusions Because vaccines against IAV are not licensed in Argentina, these results reflect the situation of IAV infection in non-vaccinated herds. This study provides more information about the circulation and characteristics of IAV in a poorly surveyed region. This study provides more data that will be used to evaluate the tools necessary to control this disease.Fil: Dibárbora, Marina. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigación en Ciencias Veterinarias y Agronómicas; Argentina. Instituto Nacional de Tecnología Agropecuaria; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Cappuccio, Javier Alejandro. Universidad Nacional de la Plata. Facultad de Ciencias Veterinarias. Catedra de Patologia Especial; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Olivera, Valeria. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigación en Ciencias Veterinarias y Agronómicas; Argentina. Instituto Nacional de Tecnología Agropecuaria; ArgentinaFil: Quiroga, Maria. Universidad Nacional de la Plata. Facultad de Ciencias Veterinarias. Catedra de Patologia Especial; ArgentinaFil: Machuca, Mariana Alejandra. Universidad Nacional de la Plata. Facultad de Ciencias Veterinarias. Catedra de Patologia Especial; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Perfumo, Carlos Juan. Universidad Nacional de la Plata. Facultad de Ciencias Veterinarias. Catedra de Patologia Especial; ArgentinaFil: Pérez, Daniel Roberto. University of Maryland; Estados UnidosFil: Pereda, Ariel Julián. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigación en Ciencias Veterinarias y Agronómicas; Argentina. Instituto Nacional de Tecnología Agropecuaria; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaWiley Blackwell Publishing, Inc2013-11-14info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/24278Dibárbora, Marina; Cappuccio, Javier Alejandro; Olivera, Valeria; Quiroga, Maria; Machuca, Mariana Alejandra; et al.; Swine influenza: clinical, serological, pathological, and virological cross-sectional studies in nine farms in Argentina; Wiley Blackwell Publishing, Inc; Influenza And Other Respiratory Viruses; 7; 4; 14-11-2013; 10-151750-2640CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/http://onlinelibrary.wiley.com/doi/10.1111/irv.12200/abstractinfo:eu-repo/semantics/altIdentifier/doi/10.1111/irv.12200info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-03T09:46:44Zoai:ri.conicet.gov.ar:11336/24278instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-03 09:46:44.639CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Swine influenza: clinical, serological, pathological, and virological cross-sectional studies in nine farms in Argentina
title Swine influenza: clinical, serological, pathological, and virological cross-sectional studies in nine farms in Argentina
spellingShingle Swine influenza: clinical, serological, pathological, and virological cross-sectional studies in nine farms in Argentina
Dibárbora, Marina
ARGENTINA
INFLUENZA
PATHOLOGY
SEROLOGY
SWINE
VIROLOGY
title_short Swine influenza: clinical, serological, pathological, and virological cross-sectional studies in nine farms in Argentina
title_full Swine influenza: clinical, serological, pathological, and virological cross-sectional studies in nine farms in Argentina
title_fullStr Swine influenza: clinical, serological, pathological, and virological cross-sectional studies in nine farms in Argentina
title_full_unstemmed Swine influenza: clinical, serological, pathological, and virological cross-sectional studies in nine farms in Argentina
title_sort Swine influenza: clinical, serological, pathological, and virological cross-sectional studies in nine farms in Argentina
dc.creator.none.fl_str_mv Dibárbora, Marina
Cappuccio, Javier Alejandro
Olivera, Valeria
Quiroga, Maria
Machuca, Mariana Alejandra
Perfumo, Carlos Juan
Pérez, Daniel Roberto
Pereda, Ariel Julián
author Dibárbora, Marina
author_facet Dibárbora, Marina
Cappuccio, Javier Alejandro
Olivera, Valeria
Quiroga, Maria
Machuca, Mariana Alejandra
Perfumo, Carlos Juan
Pérez, Daniel Roberto
Pereda, Ariel Julián
author_role author
author2 Cappuccio, Javier Alejandro
Olivera, Valeria
Quiroga, Maria
Machuca, Mariana Alejandra
Perfumo, Carlos Juan
Pérez, Daniel Roberto
Pereda, Ariel Julián
author2_role author
author
author
author
author
author
author
dc.subject.none.fl_str_mv ARGENTINA
INFLUENZA
PATHOLOGY
SEROLOGY
SWINE
VIROLOGY
topic ARGENTINA
INFLUENZA
PATHOLOGY
SEROLOGY
SWINE
VIROLOGY
dc.description.none.fl_txt_mv Background Influenza A viruses (IAV) are important pathogens responsible for economic losses in the swine industry and represent a threat to public health. In Argentina, clinical, pathological, and virological findings suggest that IAV infection is widespread among pig farms. In addition, several subtypes of IAV, such as pH1N1, H3N2, δ1H1N1, and δ2H1N2, have been reported. Objectives To evaluate the infection patterns of influenza virus in nine pig farms in Argentina. Methods Clinical, serological, pathological, and virological cross-sectional studies were conducted. Results Clinical and pathological results were characteristic of endemic influenza infection in eight of the nine farms studied. By rRT-PCR, six of the nine farms were positive to influenza. Five IAV were obtained. Genome analysis determined that four of the isolations were pH1N1 and that the remaining one was a reassortant human origin H3N2 virus containing pandemic internal genes. Serological results showed that all farms were positive to influenza A antibodies. Moreover, the hemagglutination inhibition test showed that infection with viruses containing HA′s from different subtypes (pH1, δ1H1, δ2H1, and H3) is present among the farms studied and that coinfections with two or more subtypes were present in 80.5% of positive pigs. Conclusions Because vaccines against IAV are not licensed in Argentina, these results reflect the situation of IAV infection in non-vaccinated herds. This study provides more information about the circulation and characteristics of IAV in a poorly surveyed region. This study provides more data that will be used to evaluate the tools necessary to control this disease.
Fil: Dibárbora, Marina. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigación en Ciencias Veterinarias y Agronómicas; Argentina. Instituto Nacional de Tecnología Agropecuaria; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Cappuccio, Javier Alejandro. Universidad Nacional de la Plata. Facultad de Ciencias Veterinarias. Catedra de Patologia Especial; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Olivera, Valeria. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigación en Ciencias Veterinarias y Agronómicas; Argentina. Instituto Nacional de Tecnología Agropecuaria; Argentina
Fil: Quiroga, Maria. Universidad Nacional de la Plata. Facultad de Ciencias Veterinarias. Catedra de Patologia Especial; Argentina
Fil: Machuca, Mariana Alejandra. Universidad Nacional de la Plata. Facultad de Ciencias Veterinarias. Catedra de Patologia Especial; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Perfumo, Carlos Juan. Universidad Nacional de la Plata. Facultad de Ciencias Veterinarias. Catedra de Patologia Especial; Argentina
Fil: Pérez, Daniel Roberto. University of Maryland; Estados Unidos
Fil: Pereda, Ariel Julián. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigación en Ciencias Veterinarias y Agronómicas; Argentina. Instituto Nacional de Tecnología Agropecuaria; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
description Background Influenza A viruses (IAV) are important pathogens responsible for economic losses in the swine industry and represent a threat to public health. In Argentina, clinical, pathological, and virological findings suggest that IAV infection is widespread among pig farms. In addition, several subtypes of IAV, such as pH1N1, H3N2, δ1H1N1, and δ2H1N2, have been reported. Objectives To evaluate the infection patterns of influenza virus in nine pig farms in Argentina. Methods Clinical, serological, pathological, and virological cross-sectional studies were conducted. Results Clinical and pathological results were characteristic of endemic influenza infection in eight of the nine farms studied. By rRT-PCR, six of the nine farms were positive to influenza. Five IAV were obtained. Genome analysis determined that four of the isolations were pH1N1 and that the remaining one was a reassortant human origin H3N2 virus containing pandemic internal genes. Serological results showed that all farms were positive to influenza A antibodies. Moreover, the hemagglutination inhibition test showed that infection with viruses containing HA′s from different subtypes (pH1, δ1H1, δ2H1, and H3) is present among the farms studied and that coinfections with two or more subtypes were present in 80.5% of positive pigs. Conclusions Because vaccines against IAV are not licensed in Argentina, these results reflect the situation of IAV infection in non-vaccinated herds. This study provides more information about the circulation and characteristics of IAV in a poorly surveyed region. This study provides more data that will be used to evaluate the tools necessary to control this disease.
publishDate 2013
dc.date.none.fl_str_mv 2013-11-14
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/24278
Dibárbora, Marina; Cappuccio, Javier Alejandro; Olivera, Valeria; Quiroga, Maria; Machuca, Mariana Alejandra; et al.; Swine influenza: clinical, serological, pathological, and virological cross-sectional studies in nine farms in Argentina; Wiley Blackwell Publishing, Inc; Influenza And Other Respiratory Viruses; 7; 4; 14-11-2013; 10-15
1750-2640
CONICET Digital
CONICET
url http://hdl.handle.net/11336/24278
identifier_str_mv Dibárbora, Marina; Cappuccio, Javier Alejandro; Olivera, Valeria; Quiroga, Maria; Machuca, Mariana Alejandra; et al.; Swine influenza: clinical, serological, pathological, and virological cross-sectional studies in nine farms in Argentina; Wiley Blackwell Publishing, Inc; Influenza And Other Respiratory Viruses; 7; 4; 14-11-2013; 10-15
1750-2640
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
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info:eu-repo/semantics/altIdentifier/doi/10.1111/irv.12200
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
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eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Wiley Blackwell Publishing, Inc
publisher.none.fl_str_mv Wiley Blackwell Publishing, Inc
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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