Fractions of a chloroform extract of ajenjo leaves (Artemisia mendozana DC. var. mendozana) inhibit the proliferation, viability and clonogenicity of B16-F0 melanoma cells

Autores
Millán, María Elisa; Marra, María Fernanda; Salvarredi, Leonardo Andres; Lizarraga, Emilio Fernando; Lopez, Luis Alberto
Año de publicación
2020
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
The ajenjo, Artemisia mendozana DC. var. mendozana (Asteraceae), grows in the Andean foothills of Mendoza and San Juan, Argentina, and is used as a medicinal plant for its antispasmodic and antifungal properties. The aim of this work was to obtain fractions of a chloroform extract of ajenjo leaves and to evaluate the in vitro effects on proliferation, viability and clonogenicity of B16-F0 melanoma cells. Using a silica gel chromatography column, 120 fractions were collected and grouped according to the chromatographic profile in 9 main fractions (F1-F9). Their major compounds identified were: Terpenes (F1), terpenes and sesquiterpene lactones (F2-F3), sesquiterpenes (F4-F6) and phenols and sesquiterpenes (F7-9). B16-F0 cells were incubated for 72 h with DMSO (vehicle) or 0.1 mg/ml F1-F9. At 72 h of culture, F1 decreased both the growing index (GI) and cell viability. F2 and F3 both decreased GI and only F3 decreased clonogenic activity. F4 and F5 both decreased GI. Only F5 decreased cell viability and F4 decreased clonogenicity. Consequently, fractions F6-F8 did not affect any of the cell parameters assayed, while F9 decreased cell viability and inhibited clonogenicity.
Fil: Millán, María Elisa. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Histología y Embriología de Mendoza Dr. Mario H. Burgos. Universidad Nacional de Cuyo. Facultad de Ciencias Médicas. Instituto de Histología y Embriología de Mendoza Dr. Mario H. Burgos; Argentina
Fil: Marra, María Fernanda. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Histología y Embriología de Mendoza Dr. Mario H. Burgos. Universidad Nacional de Cuyo. Facultad de Ciencias Médicas. Instituto de Histología y Embriología de Mendoza Dr. Mario H. Burgos; Argentina
Fil: Salvarredi, Leonardo Andres. Comisión Nacional de Energía Atómica; Argentina. Universidad Nacional de Cuyo; Argentina. Fundación Escuela de Medicina Nuclear; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Lizarraga, Emilio Fernando. Fundación Miguel Lillo; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional de Tucumán; Argentina
Fil: Lopez, Luis Alberto. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Histología y Embriología de Mendoza Dr. Mario H. Burgos. Universidad Nacional de Cuyo. Facultad de Ciencias Médicas. Instituto de Histología y Embriología de Mendoza Dr. Mario H. Burgos; Argentina
Materia
PHENOLS
SESQUITERPENE LACTONES
SESQUITERPENES
TERPENES
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/140983
Acceder
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spelling Fractions of a chloroform extract of ajenjo leaves (Artemisia mendozana DC. var. mendozana) inhibit the proliferation, viability and clonogenicity of B16-F0 melanoma cellsMillán, María ElisaMarra, María FernandaSalvarredi, Leonardo AndresLizarraga, Emilio FernandoLopez, Luis AlbertoPHENOLSSESQUITERPENE LACTONESSESQUITERPENESTERPENEShttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1The ajenjo, Artemisia mendozana DC. var. mendozana (Asteraceae), grows in the Andean foothills of Mendoza and San Juan, Argentina, and is used as a medicinal plant for its antispasmodic and antifungal properties. The aim of this work was to obtain fractions of a chloroform extract of ajenjo leaves and to evaluate the in vitro effects on proliferation, viability and clonogenicity of B16-F0 melanoma cells. Using a silica gel chromatography column, 120 fractions were collected and grouped according to the chromatographic profile in 9 main fractions (F1-F9). Their major compounds identified were: Terpenes (F1), terpenes and sesquiterpene lactones (F2-F3), sesquiterpenes (F4-F6) and phenols and sesquiterpenes (F7-9). B16-F0 cells were incubated for 72 h with DMSO (vehicle) or 0.1 mg/ml F1-F9. At 72 h of culture, F1 decreased both the growing index (GI) and cell viability. F2 and F3 both decreased GI and only F3 decreased clonogenic activity. F4 and F5 both decreased GI. Only F5 decreased cell viability and F4 decreased clonogenicity. Consequently, fractions F6-F8 did not affect any of the cell parameters assayed, while F9 decreased cell viability and inhibited clonogenicity.Fil: Millán, María Elisa. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Histología y Embriología de Mendoza Dr. Mario H. Burgos. Universidad Nacional de Cuyo. Facultad de Ciencias Médicas. Instituto de Histología y Embriología de Mendoza Dr. Mario H. Burgos; ArgentinaFil: Marra, María Fernanda. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Histología y Embriología de Mendoza Dr. Mario H. Burgos. Universidad Nacional de Cuyo. Facultad de Ciencias Médicas. Instituto de Histología y Embriología de Mendoza Dr. Mario H. Burgos; ArgentinaFil: Salvarredi, Leonardo Andres. Comisión Nacional de Energía Atómica; Argentina. Universidad Nacional de Cuyo; Argentina. Fundación Escuela de Medicina Nuclear; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Lizarraga, Emilio Fernando. Fundación Miguel Lillo; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional de Tucumán; ArgentinaFil: Lopez, Luis Alberto. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Histología y Embriología de Mendoza Dr. Mario H. Burgos. Universidad Nacional de Cuyo. Facultad de Ciencias Médicas. Instituto de Histología y Embriología de Mendoza Dr. Mario H. Burgos; ArgentinaInstituto de Histología y Embriología2020-09info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/140983Millán, María Elisa; Marra, María Fernanda; Salvarredi, Leonardo Andres; Lizarraga, Emilio Fernando; Lopez, Luis Alberto; Fractions of a chloroform extract of ajenjo leaves (Artemisia mendozana DC. var. mendozana) inhibit the proliferation, viability and clonogenicity of B16-F0 melanoma cells; Instituto de Histología y Embriología; Biocell; 44; 3; 9-2020; 293-2990327-9545CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.32604/biocell.2020.012342info:eu-repo/semantics/altIdentifier/url/https://www.techscience.com/biocell/v44n3/40228info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T09:49:23Zoai:ri.conicet.gov.ar:11336/140983instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 09:49:24.217CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Fractions of a chloroform extract of ajenjo leaves (Artemisia mendozana DC. var. mendozana) inhibit the proliferation, viability and clonogenicity of B16-F0 melanoma cells
title Fractions of a chloroform extract of ajenjo leaves (Artemisia mendozana DC. var. mendozana) inhibit the proliferation, viability and clonogenicity of B16-F0 melanoma cells
spellingShingle Fractions of a chloroform extract of ajenjo leaves (Artemisia mendozana DC. var. mendozana) inhibit the proliferation, viability and clonogenicity of B16-F0 melanoma cells
Millán, María Elisa
PHENOLS
SESQUITERPENE LACTONES
SESQUITERPENES
TERPENES
title_short Fractions of a chloroform extract of ajenjo leaves (Artemisia mendozana DC. var. mendozana) inhibit the proliferation, viability and clonogenicity of B16-F0 melanoma cells
title_full Fractions of a chloroform extract of ajenjo leaves (Artemisia mendozana DC. var. mendozana) inhibit the proliferation, viability and clonogenicity of B16-F0 melanoma cells
title_fullStr Fractions of a chloroform extract of ajenjo leaves (Artemisia mendozana DC. var. mendozana) inhibit the proliferation, viability and clonogenicity of B16-F0 melanoma cells
title_full_unstemmed Fractions of a chloroform extract of ajenjo leaves (Artemisia mendozana DC. var. mendozana) inhibit the proliferation, viability and clonogenicity of B16-F0 melanoma cells
title_sort Fractions of a chloroform extract of ajenjo leaves (Artemisia mendozana DC. var. mendozana) inhibit the proliferation, viability and clonogenicity of B16-F0 melanoma cells
dc.creator.none.fl_str_mv Millán, María Elisa
Marra, María Fernanda
Salvarredi, Leonardo Andres
Lizarraga, Emilio Fernando
Lopez, Luis Alberto
author Millán, María Elisa
author_facet Millán, María Elisa
Marra, María Fernanda
Salvarredi, Leonardo Andres
Lizarraga, Emilio Fernando
Lopez, Luis Alberto
author_role author
author2 Marra, María Fernanda
Salvarredi, Leonardo Andres
Lizarraga, Emilio Fernando
Lopez, Luis Alberto
author2_role author
author
author
author
dc.subject.none.fl_str_mv PHENOLS
SESQUITERPENE LACTONES
SESQUITERPENES
TERPENES
topic PHENOLS
SESQUITERPENE LACTONES
SESQUITERPENES
TERPENES
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv The ajenjo, Artemisia mendozana DC. var. mendozana (Asteraceae), grows in the Andean foothills of Mendoza and San Juan, Argentina, and is used as a medicinal plant for its antispasmodic and antifungal properties. The aim of this work was to obtain fractions of a chloroform extract of ajenjo leaves and to evaluate the in vitro effects on proliferation, viability and clonogenicity of B16-F0 melanoma cells. Using a silica gel chromatography column, 120 fractions were collected and grouped according to the chromatographic profile in 9 main fractions (F1-F9). Their major compounds identified were: Terpenes (F1), terpenes and sesquiterpene lactones (F2-F3), sesquiterpenes (F4-F6) and phenols and sesquiterpenes (F7-9). B16-F0 cells were incubated for 72 h with DMSO (vehicle) or 0.1 mg/ml F1-F9. At 72 h of culture, F1 decreased both the growing index (GI) and cell viability. F2 and F3 both decreased GI and only F3 decreased clonogenic activity. F4 and F5 both decreased GI. Only F5 decreased cell viability and F4 decreased clonogenicity. Consequently, fractions F6-F8 did not affect any of the cell parameters assayed, while F9 decreased cell viability and inhibited clonogenicity.
Fil: Millán, María Elisa. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Histología y Embriología de Mendoza Dr. Mario H. Burgos. Universidad Nacional de Cuyo. Facultad de Ciencias Médicas. Instituto de Histología y Embriología de Mendoza Dr. Mario H. Burgos; Argentina
Fil: Marra, María Fernanda. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Histología y Embriología de Mendoza Dr. Mario H. Burgos. Universidad Nacional de Cuyo. Facultad de Ciencias Médicas. Instituto de Histología y Embriología de Mendoza Dr. Mario H. Burgos; Argentina
Fil: Salvarredi, Leonardo Andres. Comisión Nacional de Energía Atómica; Argentina. Universidad Nacional de Cuyo; Argentina. Fundación Escuela de Medicina Nuclear; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Lizarraga, Emilio Fernando. Fundación Miguel Lillo; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional de Tucumán; Argentina
Fil: Lopez, Luis Alberto. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Histología y Embriología de Mendoza Dr. Mario H. Burgos. Universidad Nacional de Cuyo. Facultad de Ciencias Médicas. Instituto de Histología y Embriología de Mendoza Dr. Mario H. Burgos; Argentina
description The ajenjo, Artemisia mendozana DC. var. mendozana (Asteraceae), grows in the Andean foothills of Mendoza and San Juan, Argentina, and is used as a medicinal plant for its antispasmodic and antifungal properties. The aim of this work was to obtain fractions of a chloroform extract of ajenjo leaves and to evaluate the in vitro effects on proliferation, viability and clonogenicity of B16-F0 melanoma cells. Using a silica gel chromatography column, 120 fractions were collected and grouped according to the chromatographic profile in 9 main fractions (F1-F9). Their major compounds identified were: Terpenes (F1), terpenes and sesquiterpene lactones (F2-F3), sesquiterpenes (F4-F6) and phenols and sesquiterpenes (F7-9). B16-F0 cells were incubated for 72 h with DMSO (vehicle) or 0.1 mg/ml F1-F9. At 72 h of culture, F1 decreased both the growing index (GI) and cell viability. F2 and F3 both decreased GI and only F3 decreased clonogenic activity. F4 and F5 both decreased GI. Only F5 decreased cell viability and F4 decreased clonogenicity. Consequently, fractions F6-F8 did not affect any of the cell parameters assayed, while F9 decreased cell viability and inhibited clonogenicity.
publishDate 2020
dc.date.none.fl_str_mv 2020-09
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/140983
Millán, María Elisa; Marra, María Fernanda; Salvarredi, Leonardo Andres; Lizarraga, Emilio Fernando; Lopez, Luis Alberto; Fractions of a chloroform extract of ajenjo leaves (Artemisia mendozana DC. var. mendozana) inhibit the proliferation, viability and clonogenicity of B16-F0 melanoma cells; Instituto de Histología y Embriología; Biocell; 44; 3; 9-2020; 293-299
0327-9545
CONICET Digital
CONICET
url http://hdl.handle.net/11336/140983
identifier_str_mv Millán, María Elisa; Marra, María Fernanda; Salvarredi, Leonardo Andres; Lizarraga, Emilio Fernando; Lopez, Luis Alberto; Fractions of a chloroform extract of ajenjo leaves (Artemisia mendozana DC. var. mendozana) inhibit the proliferation, viability and clonogenicity of B16-F0 melanoma cells; Instituto de Histología y Embriología; Biocell; 44; 3; 9-2020; 293-299
0327-9545
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.32604/biocell.2020.012342
info:eu-repo/semantics/altIdentifier/url/https://www.techscience.com/biocell/v44n3/40228
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Instituto de Histología y Embriología
publisher.none.fl_str_mv Instituto de Histología y Embriología
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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