Protein ubiquitination in postsynaptic densities after hypoxia in rat neostriatum is blocked by hypothermia

Autores
Capani, Francisco; Saraceno, Gustavo Ezequiel; Botti, Valeria; Aon Bertolino, Maria Laura; Madureira de Oliveira, Diego; Barreto, George; Galeano, Pablo; Giraldez Alvarez, Lisandro Diego; Coirini, Hector
Año de publicación
2009
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Synaptic dysfunction has been associated with neuronal cell death following hypoxia. The lack of knowledge on the mechanisms underlying this dysfunction prompted us to investigate the morphological changes in the postsynaptic densities (PSDs) induced by hypoxia. The results presented here demonstrate that PSDs of the rat neostriatum are highly modified and ubiquitinated 6 months after induction of hypoxia in a model of perinatal asphyxia. Using both two dimensional (2D) and three dimensional (3D) electron microscopic analyses of synapses stained with ethanolic phosphotungstic acid (E-PTA), we observed an increment of PSD thickness dependent on the duration and severity of the hypoxic insult. The PSDs showed clear signs of damage and intense staining for ubiquitin. These morphological and molecular changes were effectively blocked by hypothermia treatment, one of the most effective strategies for hypoxia-induced brain injury available today. Our data suggest that synaptic dysfunction following hypoxia may be caused by long-term misfolding and aggregation of proteins in the PSD.
Fil: Capani, Francisco. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Investigaciones Cardiológicas. Universidad de Buenos Aires. Facultad de Medicina. Instituto de Investigaciones Cardiológicas; Argentina
Fil: Saraceno, Gustavo Ezequiel. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Investigaciones Cardiológicas. Universidad de Buenos Aires. Facultad de Medicina. Instituto de Investigaciones Cardiológicas; Argentina
Fil: Botti, Valeria. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biología y Medicina Experimental. Fundación de Instituto de Biología y Medicina Experimental. Instituto de Biología y Medicina Experimental; Argentina
Fil: Aon Bertolino, Maria Laura. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Investigaciones Cardiológicas. Universidad de Buenos Aires. Facultad de Medicina. Instituto de Investigaciones Cardiológicas; Argentina
Fil: Madureira de Oliveira, Diego. Universidade Federal da Bahia; Brasil
Fil: Barreto, George. Consejo Superior de Investigaciones Científicas. Instituto Cajal; España
Fil: Galeano, Pablo. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Investigaciones Cardiológicas. Universidad de Buenos Aires. Facultad de Medicina. Instituto de Investigaciones Cardiológicas; Argentina
Fil: Giraldez Alvarez, Lisandro Diego. Universidade Federal da Bahia; Brasil
Fil: Coirini, Hector. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biología y Medicina Experimental. Fundación de Instituto de Biología y Medicina Experimental. Instituto de Biología y Medicina Experimental; Argentina
Materia
Ap
Neostriatum
Synapse
Hypoxia
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/26247

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network_name_str CONICET Digital (CONICET)
spelling Protein ubiquitination in postsynaptic densities after hypoxia in rat neostriatum is blocked by hypothermiaCapani, FranciscoSaraceno, Gustavo EzequielBotti, ValeriaAon Bertolino, Maria LauraMadureira de Oliveira, DiegoBarreto, GeorgeGaleano, PabloGiraldez Alvarez, Lisandro DiegoCoirini, HectorApNeostriatumSynapseHypoxiahttps://purl.org/becyt/ford/3.1https://purl.org/becyt/ford/3Synaptic dysfunction has been associated with neuronal cell death following hypoxia. The lack of knowledge on the mechanisms underlying this dysfunction prompted us to investigate the morphological changes in the postsynaptic densities (PSDs) induced by hypoxia. The results presented here demonstrate that PSDs of the rat neostriatum are highly modified and ubiquitinated 6 months after induction of hypoxia in a model of perinatal asphyxia. Using both two dimensional (2D) and three dimensional (3D) electron microscopic analyses of synapses stained with ethanolic phosphotungstic acid (E-PTA), we observed an increment of PSD thickness dependent on the duration and severity of the hypoxic insult. The PSDs showed clear signs of damage and intense staining for ubiquitin. These morphological and molecular changes were effectively blocked by hypothermia treatment, one of the most effective strategies for hypoxia-induced brain injury available today. Our data suggest that synaptic dysfunction following hypoxia may be caused by long-term misfolding and aggregation of proteins in the PSD.Fil: Capani, Francisco. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Investigaciones Cardiológicas. Universidad de Buenos Aires. Facultad de Medicina. Instituto de Investigaciones Cardiológicas; ArgentinaFil: Saraceno, Gustavo Ezequiel. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Investigaciones Cardiológicas. Universidad de Buenos Aires. Facultad de Medicina. Instituto de Investigaciones Cardiológicas; ArgentinaFil: Botti, Valeria. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biología y Medicina Experimental. Fundación de Instituto de Biología y Medicina Experimental. Instituto de Biología y Medicina Experimental; ArgentinaFil: Aon Bertolino, Maria Laura. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Investigaciones Cardiológicas. Universidad de Buenos Aires. Facultad de Medicina. Instituto de Investigaciones Cardiológicas; ArgentinaFil: Madureira de Oliveira, Diego. Universidade Federal da Bahia; BrasilFil: Barreto, George. Consejo Superior de Investigaciones Científicas. Instituto Cajal; EspañaFil: Galeano, Pablo. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Investigaciones Cardiológicas. Universidad de Buenos Aires. Facultad de Medicina. Instituto de Investigaciones Cardiológicas; ArgentinaFil: Giraldez Alvarez, Lisandro Diego. Universidade Federal da Bahia; BrasilFil: Coirini, Hector. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biología y Medicina Experimental. Fundación de Instituto de Biología y Medicina Experimental. Instituto de Biología y Medicina Experimental; ArgentinaElsevier2009info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/26247Capani, Francisco; Saraceno, Gustavo Ezequiel; Botti, Valeria; Aon Bertolino, Maria Laura; Madureira de Oliveira, Diego; et al.; Protein ubiquitination in postsynaptic densities after hypoxia in rat neostriatum is blocked by hypothermia; Elsevier; Experimental Neurology; 219; 2; -1-2009; 404-4130014-48861090-2430CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/http://www.sciencedirect.com/science/article/pii/S0014488609002362info:eu-repo/semantics/altIdentifier/doi/10.1016/j.expneurol.2009.06.007info:eu-repo/semantics/altIdentifier/pmid/19555686info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T09:37:41Zoai:ri.conicet.gov.ar:11336/26247instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 09:37:41.93CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Protein ubiquitination in postsynaptic densities after hypoxia in rat neostriatum is blocked by hypothermia
title Protein ubiquitination in postsynaptic densities after hypoxia in rat neostriatum is blocked by hypothermia
spellingShingle Protein ubiquitination in postsynaptic densities after hypoxia in rat neostriatum is blocked by hypothermia
Capani, Francisco
Ap
Neostriatum
Synapse
Hypoxia
title_short Protein ubiquitination in postsynaptic densities after hypoxia in rat neostriatum is blocked by hypothermia
title_full Protein ubiquitination in postsynaptic densities after hypoxia in rat neostriatum is blocked by hypothermia
title_fullStr Protein ubiquitination in postsynaptic densities after hypoxia in rat neostriatum is blocked by hypothermia
title_full_unstemmed Protein ubiquitination in postsynaptic densities after hypoxia in rat neostriatum is blocked by hypothermia
title_sort Protein ubiquitination in postsynaptic densities after hypoxia in rat neostriatum is blocked by hypothermia
dc.creator.none.fl_str_mv Capani, Francisco
Saraceno, Gustavo Ezequiel
Botti, Valeria
Aon Bertolino, Maria Laura
Madureira de Oliveira, Diego
Barreto, George
Galeano, Pablo
Giraldez Alvarez, Lisandro Diego
Coirini, Hector
author Capani, Francisco
author_facet Capani, Francisco
Saraceno, Gustavo Ezequiel
Botti, Valeria
Aon Bertolino, Maria Laura
Madureira de Oliveira, Diego
Barreto, George
Galeano, Pablo
Giraldez Alvarez, Lisandro Diego
Coirini, Hector
author_role author
author2 Saraceno, Gustavo Ezequiel
Botti, Valeria
Aon Bertolino, Maria Laura
Madureira de Oliveira, Diego
Barreto, George
Galeano, Pablo
Giraldez Alvarez, Lisandro Diego
Coirini, Hector
author2_role author
author
author
author
author
author
author
author
dc.subject.none.fl_str_mv Ap
Neostriatum
Synapse
Hypoxia
topic Ap
Neostriatum
Synapse
Hypoxia
purl_subject.fl_str_mv https://purl.org/becyt/ford/3.1
https://purl.org/becyt/ford/3
dc.description.none.fl_txt_mv Synaptic dysfunction has been associated with neuronal cell death following hypoxia. The lack of knowledge on the mechanisms underlying this dysfunction prompted us to investigate the morphological changes in the postsynaptic densities (PSDs) induced by hypoxia. The results presented here demonstrate that PSDs of the rat neostriatum are highly modified and ubiquitinated 6 months after induction of hypoxia in a model of perinatal asphyxia. Using both two dimensional (2D) and three dimensional (3D) electron microscopic analyses of synapses stained with ethanolic phosphotungstic acid (E-PTA), we observed an increment of PSD thickness dependent on the duration and severity of the hypoxic insult. The PSDs showed clear signs of damage and intense staining for ubiquitin. These morphological and molecular changes were effectively blocked by hypothermia treatment, one of the most effective strategies for hypoxia-induced brain injury available today. Our data suggest that synaptic dysfunction following hypoxia may be caused by long-term misfolding and aggregation of proteins in the PSD.
Fil: Capani, Francisco. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Investigaciones Cardiológicas. Universidad de Buenos Aires. Facultad de Medicina. Instituto de Investigaciones Cardiológicas; Argentina
Fil: Saraceno, Gustavo Ezequiel. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Investigaciones Cardiológicas. Universidad de Buenos Aires. Facultad de Medicina. Instituto de Investigaciones Cardiológicas; Argentina
Fil: Botti, Valeria. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biología y Medicina Experimental. Fundación de Instituto de Biología y Medicina Experimental. Instituto de Biología y Medicina Experimental; Argentina
Fil: Aon Bertolino, Maria Laura. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Investigaciones Cardiológicas. Universidad de Buenos Aires. Facultad de Medicina. Instituto de Investigaciones Cardiológicas; Argentina
Fil: Madureira de Oliveira, Diego. Universidade Federal da Bahia; Brasil
Fil: Barreto, George. Consejo Superior de Investigaciones Científicas. Instituto Cajal; España
Fil: Galeano, Pablo. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Investigaciones Cardiológicas. Universidad de Buenos Aires. Facultad de Medicina. Instituto de Investigaciones Cardiológicas; Argentina
Fil: Giraldez Alvarez, Lisandro Diego. Universidade Federal da Bahia; Brasil
Fil: Coirini, Hector. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biología y Medicina Experimental. Fundación de Instituto de Biología y Medicina Experimental. Instituto de Biología y Medicina Experimental; Argentina
description Synaptic dysfunction has been associated with neuronal cell death following hypoxia. The lack of knowledge on the mechanisms underlying this dysfunction prompted us to investigate the morphological changes in the postsynaptic densities (PSDs) induced by hypoxia. The results presented here demonstrate that PSDs of the rat neostriatum are highly modified and ubiquitinated 6 months after induction of hypoxia in a model of perinatal asphyxia. Using both two dimensional (2D) and three dimensional (3D) electron microscopic analyses of synapses stained with ethanolic phosphotungstic acid (E-PTA), we observed an increment of PSD thickness dependent on the duration and severity of the hypoxic insult. The PSDs showed clear signs of damage and intense staining for ubiquitin. These morphological and molecular changes were effectively blocked by hypothermia treatment, one of the most effective strategies for hypoxia-induced brain injury available today. Our data suggest that synaptic dysfunction following hypoxia may be caused by long-term misfolding and aggregation of proteins in the PSD.
publishDate 2009
dc.date.none.fl_str_mv 2009
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/26247
Capani, Francisco; Saraceno, Gustavo Ezequiel; Botti, Valeria; Aon Bertolino, Maria Laura; Madureira de Oliveira, Diego; et al.; Protein ubiquitination in postsynaptic densities after hypoxia in rat neostriatum is blocked by hypothermia; Elsevier; Experimental Neurology; 219; 2; -1-2009; 404-413
0014-4886
1090-2430
CONICET Digital
CONICET
url http://hdl.handle.net/11336/26247
identifier_str_mv Capani, Francisco; Saraceno, Gustavo Ezequiel; Botti, Valeria; Aon Bertolino, Maria Laura; Madureira de Oliveira, Diego; et al.; Protein ubiquitination in postsynaptic densities after hypoxia in rat neostriatum is blocked by hypothermia; Elsevier; Experimental Neurology; 219; 2; -1-2009; 404-413
0014-4886
1090-2430
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/http://www.sciencedirect.com/science/article/pii/S0014488609002362
info:eu-repo/semantics/altIdentifier/doi/10.1016/j.expneurol.2009.06.007
info:eu-repo/semantics/altIdentifier/pmid/19555686
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Elsevier
publisher.none.fl_str_mv Elsevier
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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