Protein ubiquitination in postsynaptic densities after hypoxia in rat neostriatum is blocked by hypothermia
- Autores
- Capani, Francisco; Saraceno, Gustavo Ezequiel; Botti, Valeria; Aon Bertolino, Maria Laura; Madureira de Oliveira, Diego; Barreto, George; Galeano, Pablo; Giraldez Alvarez, Lisandro Diego; Coirini, Hector
- Año de publicación
- 2009
- Idioma
- inglés
- Tipo de recurso
- artículo
- Estado
- versión publicada
- Descripción
- Synaptic dysfunction has been associated with neuronal cell death following hypoxia. The lack of knowledge on the mechanisms underlying this dysfunction prompted us to investigate the morphological changes in the postsynaptic densities (PSDs) induced by hypoxia. The results presented here demonstrate that PSDs of the rat neostriatum are highly modified and ubiquitinated 6 months after induction of hypoxia in a model of perinatal asphyxia. Using both two dimensional (2D) and three dimensional (3D) electron microscopic analyses of synapses stained with ethanolic phosphotungstic acid (E-PTA), we observed an increment of PSD thickness dependent on the duration and severity of the hypoxic insult. The PSDs showed clear signs of damage and intense staining for ubiquitin. These morphological and molecular changes were effectively blocked by hypothermia treatment, one of the most effective strategies for hypoxia-induced brain injury available today. Our data suggest that synaptic dysfunction following hypoxia may be caused by long-term misfolding and aggregation of proteins in the PSD.
Fil: Capani, Francisco. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Investigaciones Cardiológicas. Universidad de Buenos Aires. Facultad de Medicina. Instituto de Investigaciones Cardiológicas; Argentina
Fil: Saraceno, Gustavo Ezequiel. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Investigaciones Cardiológicas. Universidad de Buenos Aires. Facultad de Medicina. Instituto de Investigaciones Cardiológicas; Argentina
Fil: Botti, Valeria. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biología y Medicina Experimental. Fundación de Instituto de Biología y Medicina Experimental. Instituto de Biología y Medicina Experimental; Argentina
Fil: Aon Bertolino, Maria Laura. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Investigaciones Cardiológicas. Universidad de Buenos Aires. Facultad de Medicina. Instituto de Investigaciones Cardiológicas; Argentina
Fil: Madureira de Oliveira, Diego. Universidade Federal da Bahia; Brasil
Fil: Barreto, George. Consejo Superior de Investigaciones Científicas. Instituto Cajal; España
Fil: Galeano, Pablo. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Investigaciones Cardiológicas. Universidad de Buenos Aires. Facultad de Medicina. Instituto de Investigaciones Cardiológicas; Argentina
Fil: Giraldez Alvarez, Lisandro Diego. Universidade Federal da Bahia; Brasil
Fil: Coirini, Hector. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biología y Medicina Experimental. Fundación de Instituto de Biología y Medicina Experimental. Instituto de Biología y Medicina Experimental; Argentina - Materia
-
Ap
Neostriatum
Synapse
Hypoxia - Nivel de accesibilidad
- acceso abierto
- Condiciones de uso
- https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
- Repositorio
- Institución
- Consejo Nacional de Investigaciones Científicas y Técnicas
- OAI Identificador
- oai:ri.conicet.gov.ar:11336/26247
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Protein ubiquitination in postsynaptic densities after hypoxia in rat neostriatum is blocked by hypothermiaCapani, FranciscoSaraceno, Gustavo EzequielBotti, ValeriaAon Bertolino, Maria LauraMadureira de Oliveira, DiegoBarreto, GeorgeGaleano, PabloGiraldez Alvarez, Lisandro DiegoCoirini, HectorApNeostriatumSynapseHypoxiahttps://purl.org/becyt/ford/3.1https://purl.org/becyt/ford/3Synaptic dysfunction has been associated with neuronal cell death following hypoxia. The lack of knowledge on the mechanisms underlying this dysfunction prompted us to investigate the morphological changes in the postsynaptic densities (PSDs) induced by hypoxia. The results presented here demonstrate that PSDs of the rat neostriatum are highly modified and ubiquitinated 6 months after induction of hypoxia in a model of perinatal asphyxia. Using both two dimensional (2D) and three dimensional (3D) electron microscopic analyses of synapses stained with ethanolic phosphotungstic acid (E-PTA), we observed an increment of PSD thickness dependent on the duration and severity of the hypoxic insult. The PSDs showed clear signs of damage and intense staining for ubiquitin. These morphological and molecular changes were effectively blocked by hypothermia treatment, one of the most effective strategies for hypoxia-induced brain injury available today. Our data suggest that synaptic dysfunction following hypoxia may be caused by long-term misfolding and aggregation of proteins in the PSD.Fil: Capani, Francisco. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Investigaciones Cardiológicas. Universidad de Buenos Aires. Facultad de Medicina. Instituto de Investigaciones Cardiológicas; ArgentinaFil: Saraceno, Gustavo Ezequiel. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Investigaciones Cardiológicas. Universidad de Buenos Aires. Facultad de Medicina. Instituto de Investigaciones Cardiológicas; ArgentinaFil: Botti, Valeria. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biología y Medicina Experimental. Fundación de Instituto de Biología y Medicina Experimental. Instituto de Biología y Medicina Experimental; ArgentinaFil: Aon Bertolino, Maria Laura. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Investigaciones Cardiológicas. Universidad de Buenos Aires. Facultad de Medicina. Instituto de Investigaciones Cardiológicas; ArgentinaFil: Madureira de Oliveira, Diego. Universidade Federal da Bahia; BrasilFil: Barreto, George. Consejo Superior de Investigaciones Científicas. Instituto Cajal; EspañaFil: Galeano, Pablo. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Investigaciones Cardiológicas. Universidad de Buenos Aires. Facultad de Medicina. Instituto de Investigaciones Cardiológicas; ArgentinaFil: Giraldez Alvarez, Lisandro Diego. Universidade Federal da Bahia; BrasilFil: Coirini, Hector. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biología y Medicina Experimental. Fundación de Instituto de Biología y Medicina Experimental. Instituto de Biología y Medicina Experimental; ArgentinaElsevier2009info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/26247Capani, Francisco; Saraceno, Gustavo Ezequiel; Botti, Valeria; Aon Bertolino, Maria Laura; Madureira de Oliveira, Diego; et al.; Protein ubiquitination in postsynaptic densities after hypoxia in rat neostriatum is blocked by hypothermia; Elsevier; Experimental Neurology; 219; 2; -1-2009; 404-4130014-48861090-2430CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/http://www.sciencedirect.com/science/article/pii/S0014488609002362info:eu-repo/semantics/altIdentifier/doi/10.1016/j.expneurol.2009.06.007info:eu-repo/semantics/altIdentifier/pmid/19555686info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T09:37:41Zoai:ri.conicet.gov.ar:11336/26247instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 09:37:41.93CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse |
dc.title.none.fl_str_mv |
Protein ubiquitination in postsynaptic densities after hypoxia in rat neostriatum is blocked by hypothermia |
title |
Protein ubiquitination in postsynaptic densities after hypoxia in rat neostriatum is blocked by hypothermia |
spellingShingle |
Protein ubiquitination in postsynaptic densities after hypoxia in rat neostriatum is blocked by hypothermia Capani, Francisco Ap Neostriatum Synapse Hypoxia |
title_short |
Protein ubiquitination in postsynaptic densities after hypoxia in rat neostriatum is blocked by hypothermia |
title_full |
Protein ubiquitination in postsynaptic densities after hypoxia in rat neostriatum is blocked by hypothermia |
title_fullStr |
Protein ubiquitination in postsynaptic densities after hypoxia in rat neostriatum is blocked by hypothermia |
title_full_unstemmed |
Protein ubiquitination in postsynaptic densities after hypoxia in rat neostriatum is blocked by hypothermia |
title_sort |
Protein ubiquitination in postsynaptic densities after hypoxia in rat neostriatum is blocked by hypothermia |
dc.creator.none.fl_str_mv |
Capani, Francisco Saraceno, Gustavo Ezequiel Botti, Valeria Aon Bertolino, Maria Laura Madureira de Oliveira, Diego Barreto, George Galeano, Pablo Giraldez Alvarez, Lisandro Diego Coirini, Hector |
author |
Capani, Francisco |
author_facet |
Capani, Francisco Saraceno, Gustavo Ezequiel Botti, Valeria Aon Bertolino, Maria Laura Madureira de Oliveira, Diego Barreto, George Galeano, Pablo Giraldez Alvarez, Lisandro Diego Coirini, Hector |
author_role |
author |
author2 |
Saraceno, Gustavo Ezequiel Botti, Valeria Aon Bertolino, Maria Laura Madureira de Oliveira, Diego Barreto, George Galeano, Pablo Giraldez Alvarez, Lisandro Diego Coirini, Hector |
author2_role |
author author author author author author author author |
dc.subject.none.fl_str_mv |
Ap Neostriatum Synapse Hypoxia |
topic |
Ap Neostriatum Synapse Hypoxia |
purl_subject.fl_str_mv |
https://purl.org/becyt/ford/3.1 https://purl.org/becyt/ford/3 |
dc.description.none.fl_txt_mv |
Synaptic dysfunction has been associated with neuronal cell death following hypoxia. The lack of knowledge on the mechanisms underlying this dysfunction prompted us to investigate the morphological changes in the postsynaptic densities (PSDs) induced by hypoxia. The results presented here demonstrate that PSDs of the rat neostriatum are highly modified and ubiquitinated 6 months after induction of hypoxia in a model of perinatal asphyxia. Using both two dimensional (2D) and three dimensional (3D) electron microscopic analyses of synapses stained with ethanolic phosphotungstic acid (E-PTA), we observed an increment of PSD thickness dependent on the duration and severity of the hypoxic insult. The PSDs showed clear signs of damage and intense staining for ubiquitin. These morphological and molecular changes were effectively blocked by hypothermia treatment, one of the most effective strategies for hypoxia-induced brain injury available today. Our data suggest that synaptic dysfunction following hypoxia may be caused by long-term misfolding and aggregation of proteins in the PSD. Fil: Capani, Francisco. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Investigaciones Cardiológicas. Universidad de Buenos Aires. Facultad de Medicina. Instituto de Investigaciones Cardiológicas; Argentina Fil: Saraceno, Gustavo Ezequiel. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Investigaciones Cardiológicas. Universidad de Buenos Aires. Facultad de Medicina. Instituto de Investigaciones Cardiológicas; Argentina Fil: Botti, Valeria. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biología y Medicina Experimental. Fundación de Instituto de Biología y Medicina Experimental. Instituto de Biología y Medicina Experimental; Argentina Fil: Aon Bertolino, Maria Laura. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Investigaciones Cardiológicas. Universidad de Buenos Aires. Facultad de Medicina. Instituto de Investigaciones Cardiológicas; Argentina Fil: Madureira de Oliveira, Diego. Universidade Federal da Bahia; Brasil Fil: Barreto, George. Consejo Superior de Investigaciones Científicas. Instituto Cajal; España Fil: Galeano, Pablo. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Investigaciones Cardiológicas. Universidad de Buenos Aires. Facultad de Medicina. Instituto de Investigaciones Cardiológicas; Argentina Fil: Giraldez Alvarez, Lisandro Diego. Universidade Federal da Bahia; Brasil Fil: Coirini, Hector. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biología y Medicina Experimental. Fundación de Instituto de Biología y Medicina Experimental. Instituto de Biología y Medicina Experimental; Argentina |
description |
Synaptic dysfunction has been associated with neuronal cell death following hypoxia. The lack of knowledge on the mechanisms underlying this dysfunction prompted us to investigate the morphological changes in the postsynaptic densities (PSDs) induced by hypoxia. The results presented here demonstrate that PSDs of the rat neostriatum are highly modified and ubiquitinated 6 months after induction of hypoxia in a model of perinatal asphyxia. Using both two dimensional (2D) and three dimensional (3D) electron microscopic analyses of synapses stained with ethanolic phosphotungstic acid (E-PTA), we observed an increment of PSD thickness dependent on the duration and severity of the hypoxic insult. The PSDs showed clear signs of damage and intense staining for ubiquitin. These morphological and molecular changes were effectively blocked by hypothermia treatment, one of the most effective strategies for hypoxia-induced brain injury available today. Our data suggest that synaptic dysfunction following hypoxia may be caused by long-term misfolding and aggregation of proteins in the PSD. |
publishDate |
2009 |
dc.date.none.fl_str_mv |
2009 |
dc.type.none.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion http://purl.org/coar/resource_type/c_6501 info:ar-repo/semantics/articulo |
format |
article |
status_str |
publishedVersion |
dc.identifier.none.fl_str_mv |
http://hdl.handle.net/11336/26247 Capani, Francisco; Saraceno, Gustavo Ezequiel; Botti, Valeria; Aon Bertolino, Maria Laura; Madureira de Oliveira, Diego; et al.; Protein ubiquitination in postsynaptic densities after hypoxia in rat neostriatum is blocked by hypothermia; Elsevier; Experimental Neurology; 219; 2; -1-2009; 404-413 0014-4886 1090-2430 CONICET Digital CONICET |
url |
http://hdl.handle.net/11336/26247 |
identifier_str_mv |
Capani, Francisco; Saraceno, Gustavo Ezequiel; Botti, Valeria; Aon Bertolino, Maria Laura; Madureira de Oliveira, Diego; et al.; Protein ubiquitination in postsynaptic densities after hypoxia in rat neostriatum is blocked by hypothermia; Elsevier; Experimental Neurology; 219; 2; -1-2009; 404-413 0014-4886 1090-2430 CONICET Digital CONICET |
dc.language.none.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
info:eu-repo/semantics/altIdentifier/url/http://www.sciencedirect.com/science/article/pii/S0014488609002362 info:eu-repo/semantics/altIdentifier/doi/10.1016/j.expneurol.2009.06.007 info:eu-repo/semantics/altIdentifier/pmid/19555686 |
dc.rights.none.fl_str_mv |
info:eu-repo/semantics/openAccess https://creativecommons.org/licenses/by-nc-sa/2.5/ar/ |
eu_rights_str_mv |
openAccess |
rights_invalid_str_mv |
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/ |
dc.format.none.fl_str_mv |
application/pdf application/pdf application/pdf application/pdf application/pdf application/pdf |
dc.publisher.none.fl_str_mv |
Elsevier |
publisher.none.fl_str_mv |
Elsevier |
dc.source.none.fl_str_mv |
reponame:CONICET Digital (CONICET) instname:Consejo Nacional de Investigaciones Científicas y Técnicas |
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CONICET Digital (CONICET) |
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CONICET Digital (CONICET) |
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Consejo Nacional de Investigaciones Científicas y Técnicas |
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CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas |
repository.mail.fl_str_mv |
dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar |
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1844613189109547008 |
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13.070432 |