Oxidative stress response of scallop Aequipecten tehuelchus from Patagonia Argentina exposed to inorganic arsenic

Autores
Sturla Lompre, Julieta; Giarratano, Erica; Gil, Monica Noemi; Bertrand, Lidwina; Malanga, Gabriela Fabiana
Año de publicación
2023
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Laboratory studies were performed to assess the impact of acute arsenic (As) exposure on the Tehuelche scallop Aequipecten tehuelchus from San José gulf (Patagonia, Argentina). The As accumulation in gills, digestive gland and muscle of A. tehuelchus was analyzed after 96 h of exposure at different concentrations (0, 4, 5, 6.3 and 7.9 mg As/L). Accumulation in all tissues increased linearly with the exposure concentration, evidencing no As saturation. A 96 h median lethal concentration (LC50) value of 7.1 mg As/L was determined, characterizing this species as relatively tolerant to As. The potential effects of As were studied through the production of reactive species, enzymatic activities of catalase and glutathione-S-transferase, metallothioneins, lipid peroxidation, and lipid-soluble antioxidants. In presence of As, the 2’,7’-dichlorodihydrofluorescein diacetate oxidation rate and thiobarbituric acid reactive substances content did not show changes in any tissues. Catalase and glutathione S-transferase activities raised in gills and digestive gland and remained unchanged in muscle. Metallothioneins increased in gills and digestive gland and a decreasing pattern of α-tocopherol was observed in gills and muscle. Scallops were slightly affected by As exposure, especially at high levels, being able to counteract its effects by the induction of biotransformation pathways and antioxidant defense mechanisms.
Fil: Sturla Lompre, Julieta. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Centro Nacional Patagónico. Centro para el Estudio de Sistemas Marinos; Argentina. Universidad Nacional de la Patagonia "San Juan Bosco"; Argentina
Fil: Giarratano, Erica. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Centro Nacional Patagónico. Centro para el Estudio de Sistemas Marinos; Argentina
Fil: Gil, Monica Noemi. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Centro Nacional Patagónico. Centro para el Estudio de Sistemas Marinos; Argentina. Universidad Nacional de la Patagonia "San Juan Bosco"; Argentina
Fil: Bertrand, Lidwina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Córdoba. Centro de Investigaciones en Bioquímica Clínica e Inmunología; Argentina. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica; Argentina
Fil: Malanga, Gabriela Fabiana. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Bioquímica y Medicina Molecular. Universidad de Buenos Aires. Facultad Medicina. Instituto de Bioquímica y Medicina Molecular; Argentina
Materia
ACUTE TOXICITY
ANTIOXIDANT DEFENSE SYSTEM
BIOMARKERS
LETHAL MEDIA CONCENTRATION
METALLOID ACCUMULATION
MOLLUSK
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/227641
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/227641
network_acronym_str CONICETDig
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network_name_str CONICET Digital (CONICET)
spelling Oxidative stress response of scallop Aequipecten tehuelchus from Patagonia Argentina exposed to inorganic arsenicSturla Lompre, JulietaGiarratano, EricaGil, Monica NoemiBertrand, LidwinaMalanga, Gabriela FabianaACUTE TOXICITYANTIOXIDANT DEFENSE SYSTEMBIOMARKERSLETHAL MEDIA CONCENTRATIONMETALLOID ACCUMULATIONMOLLUSKhttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1Laboratory studies were performed to assess the impact of acute arsenic (As) exposure on the Tehuelche scallop Aequipecten tehuelchus from San José gulf (Patagonia, Argentina). The As accumulation in gills, digestive gland and muscle of A. tehuelchus was analyzed after 96 h of exposure at different concentrations (0, 4, 5, 6.3 and 7.9 mg As/L). Accumulation in all tissues increased linearly with the exposure concentration, evidencing no As saturation. A 96 h median lethal concentration (LC50) value of 7.1 mg As/L was determined, characterizing this species as relatively tolerant to As. The potential effects of As were studied through the production of reactive species, enzymatic activities of catalase and glutathione-S-transferase, metallothioneins, lipid peroxidation, and lipid-soluble antioxidants. In presence of As, the 2’,7’-dichlorodihydrofluorescein diacetate oxidation rate and thiobarbituric acid reactive substances content did not show changes in any tissues. Catalase and glutathione S-transferase activities raised in gills and digestive gland and remained unchanged in muscle. Metallothioneins increased in gills and digestive gland and a decreasing pattern of α-tocopherol was observed in gills and muscle. Scallops were slightly affected by As exposure, especially at high levels, being able to counteract its effects by the induction of biotransformation pathways and antioxidant defense mechanisms.Fil: Sturla Lompre, Julieta. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Centro Nacional Patagónico. Centro para el Estudio de Sistemas Marinos; Argentina. Universidad Nacional de la Patagonia "San Juan Bosco"; ArgentinaFil: Giarratano, Erica. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Centro Nacional Patagónico. Centro para el Estudio de Sistemas Marinos; ArgentinaFil: Gil, Monica Noemi. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Centro Nacional Patagónico. Centro para el Estudio de Sistemas Marinos; Argentina. Universidad Nacional de la Patagonia "San Juan Bosco"; ArgentinaFil: Bertrand, Lidwina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Córdoba. Centro de Investigaciones en Bioquímica Clínica e Inmunología; Argentina. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica; ArgentinaFil: Malanga, Gabriela Fabiana. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Bioquímica y Medicina Molecular. Universidad de Buenos Aires. Facultad Medicina. Instituto de Bioquímica y Medicina Molecular; ArgentinaElsevier2023-09info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/227641Sturla Lompre, Julieta; Giarratano, Erica; Gil, Monica Noemi; Bertrand, Lidwina; Malanga, Gabriela Fabiana; Oxidative stress response of scallop Aequipecten tehuelchus from Patagonia Argentina exposed to inorganic arsenic; Elsevier; Regional Studies in Marine Science; 62; 102944; 9-2023; 1-92352-4855CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/pii/S2352485523001330info:eu-repo/semantics/altIdentifier/doi/10.1016/j.rsma.2023.102944info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-nd/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-03T09:57:18Zoai:ri.conicet.gov.ar:11336/227641instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-03 09:57:18.764CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Oxidative stress response of scallop Aequipecten tehuelchus from Patagonia Argentina exposed to inorganic arsenic
title Oxidative stress response of scallop Aequipecten tehuelchus from Patagonia Argentina exposed to inorganic arsenic
spellingShingle Oxidative stress response of scallop Aequipecten tehuelchus from Patagonia Argentina exposed to inorganic arsenic
Sturla Lompre, Julieta
ACUTE TOXICITY
ANTIOXIDANT DEFENSE SYSTEM
BIOMARKERS
LETHAL MEDIA CONCENTRATION
METALLOID ACCUMULATION
MOLLUSK
title_short Oxidative stress response of scallop Aequipecten tehuelchus from Patagonia Argentina exposed to inorganic arsenic
title_full Oxidative stress response of scallop Aequipecten tehuelchus from Patagonia Argentina exposed to inorganic arsenic
title_fullStr Oxidative stress response of scallop Aequipecten tehuelchus from Patagonia Argentina exposed to inorganic arsenic
title_full_unstemmed Oxidative stress response of scallop Aequipecten tehuelchus from Patagonia Argentina exposed to inorganic arsenic
title_sort Oxidative stress response of scallop Aequipecten tehuelchus from Patagonia Argentina exposed to inorganic arsenic
dc.creator.none.fl_str_mv Sturla Lompre, Julieta
Giarratano, Erica
Gil, Monica Noemi
Bertrand, Lidwina
Malanga, Gabriela Fabiana
author Sturla Lompre, Julieta
author_facet Sturla Lompre, Julieta
Giarratano, Erica
Gil, Monica Noemi
Bertrand, Lidwina
Malanga, Gabriela Fabiana
author_role author
author2 Giarratano, Erica
Gil, Monica Noemi
Bertrand, Lidwina
Malanga, Gabriela Fabiana
author2_role author
author
author
author
dc.subject.none.fl_str_mv ACUTE TOXICITY
ANTIOXIDANT DEFENSE SYSTEM
BIOMARKERS
LETHAL MEDIA CONCENTRATION
METALLOID ACCUMULATION
MOLLUSK
topic ACUTE TOXICITY
ANTIOXIDANT DEFENSE SYSTEM
BIOMARKERS
LETHAL MEDIA CONCENTRATION
METALLOID ACCUMULATION
MOLLUSK
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv Laboratory studies were performed to assess the impact of acute arsenic (As) exposure on the Tehuelche scallop Aequipecten tehuelchus from San José gulf (Patagonia, Argentina). The As accumulation in gills, digestive gland and muscle of A. tehuelchus was analyzed after 96 h of exposure at different concentrations (0, 4, 5, 6.3 and 7.9 mg As/L). Accumulation in all tissues increased linearly with the exposure concentration, evidencing no As saturation. A 96 h median lethal concentration (LC50) value of 7.1 mg As/L was determined, characterizing this species as relatively tolerant to As. The potential effects of As were studied through the production of reactive species, enzymatic activities of catalase and glutathione-S-transferase, metallothioneins, lipid peroxidation, and lipid-soluble antioxidants. In presence of As, the 2’,7’-dichlorodihydrofluorescein diacetate oxidation rate and thiobarbituric acid reactive substances content did not show changes in any tissues. Catalase and glutathione S-transferase activities raised in gills and digestive gland and remained unchanged in muscle. Metallothioneins increased in gills and digestive gland and a decreasing pattern of α-tocopherol was observed in gills and muscle. Scallops were slightly affected by As exposure, especially at high levels, being able to counteract its effects by the induction of biotransformation pathways and antioxidant defense mechanisms.
Fil: Sturla Lompre, Julieta. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Centro Nacional Patagónico. Centro para el Estudio de Sistemas Marinos; Argentina. Universidad Nacional de la Patagonia "San Juan Bosco"; Argentina
Fil: Giarratano, Erica. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Centro Nacional Patagónico. Centro para el Estudio de Sistemas Marinos; Argentina
Fil: Gil, Monica Noemi. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Centro Nacional Patagónico. Centro para el Estudio de Sistemas Marinos; Argentina. Universidad Nacional de la Patagonia "San Juan Bosco"; Argentina
Fil: Bertrand, Lidwina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Córdoba. Centro de Investigaciones en Bioquímica Clínica e Inmunología; Argentina. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica; Argentina
Fil: Malanga, Gabriela Fabiana. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Bioquímica y Medicina Molecular. Universidad de Buenos Aires. Facultad Medicina. Instituto de Bioquímica y Medicina Molecular; Argentina
description Laboratory studies were performed to assess the impact of acute arsenic (As) exposure on the Tehuelche scallop Aequipecten tehuelchus from San José gulf (Patagonia, Argentina). The As accumulation in gills, digestive gland and muscle of A. tehuelchus was analyzed after 96 h of exposure at different concentrations (0, 4, 5, 6.3 and 7.9 mg As/L). Accumulation in all tissues increased linearly with the exposure concentration, evidencing no As saturation. A 96 h median lethal concentration (LC50) value of 7.1 mg As/L was determined, characterizing this species as relatively tolerant to As. The potential effects of As were studied through the production of reactive species, enzymatic activities of catalase and glutathione-S-transferase, metallothioneins, lipid peroxidation, and lipid-soluble antioxidants. In presence of As, the 2’,7’-dichlorodihydrofluorescein diacetate oxidation rate and thiobarbituric acid reactive substances content did not show changes in any tissues. Catalase and glutathione S-transferase activities raised in gills and digestive gland and remained unchanged in muscle. Metallothioneins increased in gills and digestive gland and a decreasing pattern of α-tocopherol was observed in gills and muscle. Scallops were slightly affected by As exposure, especially at high levels, being able to counteract its effects by the induction of biotransformation pathways and antioxidant defense mechanisms.
publishDate 2023
dc.date.none.fl_str_mv 2023-09
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/227641
Sturla Lompre, Julieta; Giarratano, Erica; Gil, Monica Noemi; Bertrand, Lidwina; Malanga, Gabriela Fabiana; Oxidative stress response of scallop Aequipecten tehuelchus from Patagonia Argentina exposed to inorganic arsenic; Elsevier; Regional Studies in Marine Science; 62; 102944; 9-2023; 1-9
2352-4855
CONICET Digital
CONICET
url http://hdl.handle.net/11336/227641
identifier_str_mv Sturla Lompre, Julieta; Giarratano, Erica; Gil, Monica Noemi; Bertrand, Lidwina; Malanga, Gabriela Fabiana; Oxidative stress response of scallop Aequipecten tehuelchus from Patagonia Argentina exposed to inorganic arsenic; Elsevier; Regional Studies in Marine Science; 62; 102944; 9-2023; 1-9
2352-4855
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/pii/S2352485523001330
info:eu-repo/semantics/altIdentifier/doi/10.1016/j.rsma.2023.102944
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Elsevier
publisher.none.fl_str_mv Elsevier
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.13397

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