Effect of acute cadmium exposure on oxidative stress and antioxidant system of the scallop Aequipecten tehuelchus

Autores
Sturla Lompre, Julieta; Giarratano, Erica; Gil, Monica Noemi; Malanga, Gabriela Fabiana
Año de publicación
2024
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
This study aimed to assess the impact of acute exposure (96 h) to Cd in gills, digestive gland and muscle of the Tehuelche scallop Aequipecten tehuelchus from San José gulf in Patagonia, Argentina. Scallops were exposed to Cd concentrations of 0, 25, 50, 100, 150, 204, 275, 371, and 500 μg/L, and mortality rates were recorded after 96 h of exposure. Surviving organisms were analyzed for the biochemical response through reactive oxygen and nitrogen species (RONS), activities of catalase (CAT) and glutathione-S-transferase (GST), metallothioneins (MT), lipid peroxidation (LPO) and liposoluble antioxidants α-tocopherol (α-T) and β-carotene (β-C). The mean lethal concentration (LC50) was 155.8 μg Cd/L, a lower value than other scallops´ species, showing that A. tehuelchus has a particular sensitivity to Cd. In the three tissues, at all exposure concentrations, there was no significant response in RONS levels, GST activity or LPO. Nevertheless, CAT activity and α-T levels decreased in the gills but increased in the digestive gland, with no significant response in the muscle. Two-way ANOVA revealed a significant interaction between Cd concentration and tissue on MT, which increased significantly in gills, decreased in digestive gland with 100 compared to 50 μg Cd/L; whereas in muscle a significant increase was observed with 25 μg Cd/L compared to control. The results show a significant effect of Cd in scallop´s gills on CAT activity and α-T levels, highlighting this tissue as the primary target against relevant concentrations of metal in seawater. The effect on digestive gland and muscle was minimal. The overall results suggest that Cd toxicity is tissue-specific. This study will help reduce the existence knowledge gap regarding potential impacts of acute exposure to Cd in a bivalve species with high ecological and commercial importance, as well as identifying the most responsive biomarkers associated with Cd stress for monitoring assessment.
Fil: Sturla Lompre, Julieta. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Centro Nacional Patagónico. Centro para el Estudio de Sistemas Marinos; Argentina
Fil: Giarratano, Erica. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Centro Nacional Patagónico. Centro para el Estudio de Sistemas Marinos; Argentina
Fil: Gil, Monica Noemi. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Centro Nacional Patagónico. Centro para el Estudio de Sistemas Marinos; Argentina
Fil: Malanga, Gabriela Fabiana. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Bioquímica y Medicina Molecular. Universidad de Buenos Aires. Facultad Medicina. Instituto de Bioquímica y Medicina Molecular; Argentina
Materia
Metal exposure
Biochemical response
Scallop tissues
Metallothioneins
Argentine patagoniaMarine bivalves
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/261474
Acceder
id CONICETDig_4d8b6479a61167eedab41fe415ad44d4
oai_identifier_str oai:ri.conicet.gov.ar:11336/261474
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Effect of acute cadmium exposure on oxidative stress and antioxidant system of the scallop Aequipecten tehuelchusSturla Lompre, JulietaGiarratano, EricaGil, Monica NoemiMalanga, Gabriela FabianaMetal exposureBiochemical responseScallop tissuesMetallothioneinsArgentine patagoniaMarine bivalveshttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1This study aimed to assess the impact of acute exposure (96 h) to Cd in gills, digestive gland and muscle of the Tehuelche scallop Aequipecten tehuelchus from San José gulf in Patagonia, Argentina. Scallops were exposed to Cd concentrations of 0, 25, 50, 100, 150, 204, 275, 371, and 500 μg/L, and mortality rates were recorded after 96 h of exposure. Surviving organisms were analyzed for the biochemical response through reactive oxygen and nitrogen species (RONS), activities of catalase (CAT) and glutathione-S-transferase (GST), metallothioneins (MT), lipid peroxidation (LPO) and liposoluble antioxidants α-tocopherol (α-T) and β-carotene (β-C). The mean lethal concentration (LC50) was 155.8 μg Cd/L, a lower value than other scallops´ species, showing that A. tehuelchus has a particular sensitivity to Cd. In the three tissues, at all exposure concentrations, there was no significant response in RONS levels, GST activity or LPO. Nevertheless, CAT activity and α-T levels decreased in the gills but increased in the digestive gland, with no significant response in the muscle. Two-way ANOVA revealed a significant interaction between Cd concentration and tissue on MT, which increased significantly in gills, decreased in digestive gland with 100 compared to 50 μg Cd/L; whereas in muscle a significant increase was observed with 25 μg Cd/L compared to control. The results show a significant effect of Cd in scallop´s gills on CAT activity and α-T levels, highlighting this tissue as the primary target against relevant concentrations of metal in seawater. The effect on digestive gland and muscle was minimal. The overall results suggest that Cd toxicity is tissue-specific. This study will help reduce the existence knowledge gap regarding potential impacts of acute exposure to Cd in a bivalve species with high ecological and commercial importance, as well as identifying the most responsive biomarkers associated with Cd stress for monitoring assessment.Fil: Sturla Lompre, Julieta. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Centro Nacional Patagónico. Centro para el Estudio de Sistemas Marinos; ArgentinaFil: Giarratano, Erica. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Centro Nacional Patagónico. Centro para el Estudio de Sistemas Marinos; ArgentinaFil: Gil, Monica Noemi. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Centro Nacional Patagónico. Centro para el Estudio de Sistemas Marinos; ArgentinaFil: Malanga, Gabriela Fabiana. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Bioquímica y Medicina Molecular. Universidad de Buenos Aires. Facultad Medicina. Instituto de Bioquímica y Medicina Molecular; ArgentinaPergamon-Elsevier Science Ltd2024-03info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/261474Sturla Lompre, Julieta; Giarratano, Erica; Gil, Monica Noemi; Malanga, Gabriela Fabiana; Effect of acute cadmium exposure on oxidative stress and antioxidant system of the scallop Aequipecten tehuelchus; Pergamon-Elsevier Science Ltd; Chemosphere; 352; 3-2024; 1-390045-6535CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://linkinghub.elsevier.com/retrieve/pii/S0045653524004053info:eu-repo/semantics/altIdentifier/doi/10.1016/j.chemosphere.2024.141512info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-10-22T12:12:12Zoai:ri.conicet.gov.ar:11336/261474instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-10-22 12:12:13.185CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Effect of acute cadmium exposure on oxidative stress and antioxidant system of the scallop Aequipecten tehuelchus
title Effect of acute cadmium exposure on oxidative stress and antioxidant system of the scallop Aequipecten tehuelchus
spellingShingle Effect of acute cadmium exposure on oxidative stress and antioxidant system of the scallop Aequipecten tehuelchus
Sturla Lompre, Julieta
Metal exposure
Biochemical response
Scallop tissues
Metallothioneins
Argentine patagoniaMarine bivalves
title_short Effect of acute cadmium exposure on oxidative stress and antioxidant system of the scallop Aequipecten tehuelchus
title_full Effect of acute cadmium exposure on oxidative stress and antioxidant system of the scallop Aequipecten tehuelchus
title_fullStr Effect of acute cadmium exposure on oxidative stress and antioxidant system of the scallop Aequipecten tehuelchus
title_full_unstemmed Effect of acute cadmium exposure on oxidative stress and antioxidant system of the scallop Aequipecten tehuelchus
title_sort Effect of acute cadmium exposure on oxidative stress and antioxidant system of the scallop Aequipecten tehuelchus
dc.creator.none.fl_str_mv Sturla Lompre, Julieta
Giarratano, Erica
Gil, Monica Noemi
Malanga, Gabriela Fabiana
author Sturla Lompre, Julieta
author_facet Sturla Lompre, Julieta
Giarratano, Erica
Gil, Monica Noemi
Malanga, Gabriela Fabiana
author_role author
author2 Giarratano, Erica
Gil, Monica Noemi
Malanga, Gabriela Fabiana
author2_role author
author
author
dc.subject.none.fl_str_mv Metal exposure
Biochemical response
Scallop tissues
Metallothioneins
Argentine patagoniaMarine bivalves
topic Metal exposure
Biochemical response
Scallop tissues
Metallothioneins
Argentine patagoniaMarine bivalves
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv This study aimed to assess the impact of acute exposure (96 h) to Cd in gills, digestive gland and muscle of the Tehuelche scallop Aequipecten tehuelchus from San José gulf in Patagonia, Argentina. Scallops were exposed to Cd concentrations of 0, 25, 50, 100, 150, 204, 275, 371, and 500 μg/L, and mortality rates were recorded after 96 h of exposure. Surviving organisms were analyzed for the biochemical response through reactive oxygen and nitrogen species (RONS), activities of catalase (CAT) and glutathione-S-transferase (GST), metallothioneins (MT), lipid peroxidation (LPO) and liposoluble antioxidants α-tocopherol (α-T) and β-carotene (β-C). The mean lethal concentration (LC50) was 155.8 μg Cd/L, a lower value than other scallops´ species, showing that A. tehuelchus has a particular sensitivity to Cd. In the three tissues, at all exposure concentrations, there was no significant response in RONS levels, GST activity or LPO. Nevertheless, CAT activity and α-T levels decreased in the gills but increased in the digestive gland, with no significant response in the muscle. Two-way ANOVA revealed a significant interaction between Cd concentration and tissue on MT, which increased significantly in gills, decreased in digestive gland with 100 compared to 50 μg Cd/L; whereas in muscle a significant increase was observed with 25 μg Cd/L compared to control. The results show a significant effect of Cd in scallop´s gills on CAT activity and α-T levels, highlighting this tissue as the primary target against relevant concentrations of metal in seawater. The effect on digestive gland and muscle was minimal. The overall results suggest that Cd toxicity is tissue-specific. This study will help reduce the existence knowledge gap regarding potential impacts of acute exposure to Cd in a bivalve species with high ecological and commercial importance, as well as identifying the most responsive biomarkers associated with Cd stress for monitoring assessment.
Fil: Sturla Lompre, Julieta. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Centro Nacional Patagónico. Centro para el Estudio de Sistemas Marinos; Argentina
Fil: Giarratano, Erica. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Centro Nacional Patagónico. Centro para el Estudio de Sistemas Marinos; Argentina
Fil: Gil, Monica Noemi. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Centro Nacional Patagónico. Centro para el Estudio de Sistemas Marinos; Argentina
Fil: Malanga, Gabriela Fabiana. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Bioquímica y Medicina Molecular. Universidad de Buenos Aires. Facultad Medicina. Instituto de Bioquímica y Medicina Molecular; Argentina
description This study aimed to assess the impact of acute exposure (96 h) to Cd in gills, digestive gland and muscle of the Tehuelche scallop Aequipecten tehuelchus from San José gulf in Patagonia, Argentina. Scallops were exposed to Cd concentrations of 0, 25, 50, 100, 150, 204, 275, 371, and 500 μg/L, and mortality rates were recorded after 96 h of exposure. Surviving organisms were analyzed for the biochemical response through reactive oxygen and nitrogen species (RONS), activities of catalase (CAT) and glutathione-S-transferase (GST), metallothioneins (MT), lipid peroxidation (LPO) and liposoluble antioxidants α-tocopherol (α-T) and β-carotene (β-C). The mean lethal concentration (LC50) was 155.8 μg Cd/L, a lower value than other scallops´ species, showing that A. tehuelchus has a particular sensitivity to Cd. In the three tissues, at all exposure concentrations, there was no significant response in RONS levels, GST activity or LPO. Nevertheless, CAT activity and α-T levels decreased in the gills but increased in the digestive gland, with no significant response in the muscle. Two-way ANOVA revealed a significant interaction between Cd concentration and tissue on MT, which increased significantly in gills, decreased in digestive gland with 100 compared to 50 μg Cd/L; whereas in muscle a significant increase was observed with 25 μg Cd/L compared to control. The results show a significant effect of Cd in scallop´s gills on CAT activity and α-T levels, highlighting this tissue as the primary target against relevant concentrations of metal in seawater. The effect on digestive gland and muscle was minimal. The overall results suggest that Cd toxicity is tissue-specific. This study will help reduce the existence knowledge gap regarding potential impacts of acute exposure to Cd in a bivalve species with high ecological and commercial importance, as well as identifying the most responsive biomarkers associated with Cd stress for monitoring assessment.
publishDate 2024
dc.date.none.fl_str_mv 2024-03
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/261474
Sturla Lompre, Julieta; Giarratano, Erica; Gil, Monica Noemi; Malanga, Gabriela Fabiana; Effect of acute cadmium exposure on oxidative stress and antioxidant system of the scallop Aequipecten tehuelchus; Pergamon-Elsevier Science Ltd; Chemosphere; 352; 3-2024; 1-39
0045-6535
CONICET Digital
CONICET
url http://hdl.handle.net/11336/261474
identifier_str_mv Sturla Lompre, Julieta; Giarratano, Erica; Gil, Monica Noemi; Malanga, Gabriela Fabiana; Effect of acute cadmium exposure on oxidative stress and antioxidant system of the scallop Aequipecten tehuelchus; Pergamon-Elsevier Science Ltd; Chemosphere; 352; 3-2024; 1-39
0045-6535
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://linkinghub.elsevier.com/retrieve/pii/S0045653524004053
info:eu-repo/semantics/altIdentifier/doi/10.1016/j.chemosphere.2024.141512
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Pergamon-Elsevier Science Ltd
publisher.none.fl_str_mv Pergamon-Elsevier Science Ltd
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 12.982451

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