Genome-wide annotation of the soybean WRKY family and functional characterization of genes involved in response to Phakopsora pachyrhizi infection

Autores
Bencke Malato, Marta; Cabreira, Caroline; Wiebke Strohm, Beatriz; Bücker Neto, Lauro; Mancini, Estefania; Osorio, Marina B.; Homrich, Milena S.; Turchetto Zolet, Andreia C.; De Carvalho, Mayra C.C.G.; Stolf, Renata; Weber, Ricardo L.M.; Westergaard, Gastón; Castagnaro, Atilio Pedro; Abdelnoor, Ricardo V.; Marcelino Guimarães, Francismar C.; Margis Pinheiro, Márcia; Bodanese Zanettini, Maria H.
Año de publicación
2014
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Background: Many previous studies have shown that soybean WRKY transcription factors are involved in the plant response to biotic and abiotic stresses. Phakopsora pachyrhizi is the causal agent of Asian Soybean Rust, one of the most important soybean diseases. There are evidences that WRKYs are involved in the resistance of some soybean genotypes against that fungus. The number of WRKY genes already annotated in soybean genome was underrepresented. In the present study, a genome-wide annotation of the soybean WRKY family was carried out and members involved in the response to P. pachyrhizi were identified.Results: As a result of a soybean genomic databases search, 182 WRKY-encoding genes were annotated and 33 putative pseudogenes identified. Genes involved in the response to P. pachyrhizi infection were identified using superSAGE, RNA-Seq of microdissected lesions and microarray experiments. Seventy-five genes were differentially expressed during fungal infection. The expression of eight WRKY genes was validated by RT-qPCR. The expression of these genes in a resistant genotype was earlier and/or stronger compared with a susceptible genotype in response to P. pachyrhizi infection. Soybean somatic embryos were transformed in order to overexpress or silence WRKY genes. Embryos overexpressing a WRKY gene were obtained, but they were unable to convert into plants. When infected with P. pachyrhizi, the leaves of the silenced transgenic line showed a higher number of lesions than the wild-type plants.Conclusions: The present study reports a genome-wide annotation of soybean WRKY family. The participation of some members in response to P. pachyrhizi infection was demonstrated. The results contribute to the elucidation of gene function and suggest the manipulation of WRKYs as a strategy to increase fungal resistance in soybean plants.
Fil: Bencke Malato, Marta. Universidade Federal do Rio Grande do Sul; Brasil
Fil: Cabreira, Caroline. Universidade Federal do Rio Grande do Sul; Brasil
Fil: Wiebke Strohm, Beatriz. Universidade Federal do Rio Grande do Sul; Brasil
Fil: Bücker Neto, Lauro. Universidade Federal do Rio Grande do Sul; Brasil
Fil: Mancini, Estefania. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Rosario. Instituto de Agrobiotecnología de Rosario; Argentina
Fil: Osorio, Marina B.. Universidade Federal do Rio Grande do Sul; Brasil
Fil: Homrich, Milena S.. Universidade Federal do Rio Grande do Sul; Brasil
Fil: Turchetto Zolet, Andreia C.. Universidade Federal do Rio Grande do Sul; Brasil
Fil: De Carvalho, Mayra C.C.G.. Ministerio da Agricultura Pecuaria e Abastecimento de Brasil. Empresa Brasileira de Pesquisa Agropecuaria; Brasil
Fil: Stolf, Renata. Ministerio da Agricultura Pecuaria e Abastecimento de Brasil. Empresa Brasileira de Pesquisa Agropecuaria; Brasil
Fil: Weber, Ricardo L.M.. Universidade Federal do Rio Grande do Sul; Brasil
Fil: Westergaard, Gastón. Instituto de Agrobiotecnología de Rosario (indear); Argentina
Fil: Castagnaro, Atilio Pedro. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Instituto de Tecnología Agroindustrial del Noroeste Argentino. Provincia de Tucumán. Ministerio de Desarrollo Productivo. Estación Experimental Agroindustrial ; Argentina
Fil: Abdelnoor, Ricardo V.. Ministerio da Agricultura Pecuaria e Abastecimento de Brasil. Empresa Brasileira de Pesquisa Agropecuaria; Brasil
Fil: Marcelino Guimarães, Francismar C.. Ministerio da Agricultura Pecuaria e Abastecimento de Brasil. Empresa Brasileira de Pesquisa Agropecuaria; Brasil
Fil: Margis Pinheiro, Márcia. Universidade Federal do Rio Grande do Sul; Brasil
Fil: Bodanese Zanettini, Maria H.. Universidade Federal do Rio Grande do Sul; Brasil
Materia
ASIAN SOYBEAN RUST
FUNCTIONAL ANALYSIS
FUNGUS RESISTANCE
GENETIC TRANSFORMATION
GLYCINE MAX
TRANSCRIPTION FACTORS
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/36572
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/36572
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Genome-wide annotation of the soybean WRKY family and functional characterization of genes involved in response to Phakopsora pachyrhizi infectionBencke Malato, MartaCabreira, CarolineWiebke Strohm, BeatrizBücker Neto, LauroMancini, EstefaniaOsorio, Marina B.Homrich, Milena S.Turchetto Zolet, Andreia C.De Carvalho, Mayra C.C.G.Stolf, RenataWeber, Ricardo L.M.Westergaard, GastónCastagnaro, Atilio PedroAbdelnoor, Ricardo V.Marcelino Guimarães, Francismar C.Margis Pinheiro, MárciaBodanese Zanettini, Maria H.ASIAN SOYBEAN RUSTFUNCTIONAL ANALYSISFUNGUS RESISTANCEGENETIC TRANSFORMATIONGLYCINE MAXTRANSCRIPTION FACTORShttps://purl.org/becyt/ford/4.4https://purl.org/becyt/ford/4Background: Many previous studies have shown that soybean WRKY transcription factors are involved in the plant response to biotic and abiotic stresses. Phakopsora pachyrhizi is the causal agent of Asian Soybean Rust, one of the most important soybean diseases. There are evidences that WRKYs are involved in the resistance of some soybean genotypes against that fungus. The number of WRKY genes already annotated in soybean genome was underrepresented. In the present study, a genome-wide annotation of the soybean WRKY family was carried out and members involved in the response to P. pachyrhizi were identified.Results: As a result of a soybean genomic databases search, 182 WRKY-encoding genes were annotated and 33 putative pseudogenes identified. Genes involved in the response to P. pachyrhizi infection were identified using superSAGE, RNA-Seq of microdissected lesions and microarray experiments. Seventy-five genes were differentially expressed during fungal infection. The expression of eight WRKY genes was validated by RT-qPCR. The expression of these genes in a resistant genotype was earlier and/or stronger compared with a susceptible genotype in response to P. pachyrhizi infection. Soybean somatic embryos were transformed in order to overexpress or silence WRKY genes. Embryos overexpressing a WRKY gene were obtained, but they were unable to convert into plants. When infected with P. pachyrhizi, the leaves of the silenced transgenic line showed a higher number of lesions than the wild-type plants.Conclusions: The present study reports a genome-wide annotation of soybean WRKY family. The participation of some members in response to P. pachyrhizi infection was demonstrated. The results contribute to the elucidation of gene function and suggest the manipulation of WRKYs as a strategy to increase fungal resistance in soybean plants.Fil: Bencke Malato, Marta. Universidade Federal do Rio Grande do Sul; BrasilFil: Cabreira, Caroline. Universidade Federal do Rio Grande do Sul; BrasilFil: Wiebke Strohm, Beatriz. Universidade Federal do Rio Grande do Sul; BrasilFil: Bücker Neto, Lauro. Universidade Federal do Rio Grande do Sul; BrasilFil: Mancini, Estefania. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Rosario. Instituto de Agrobiotecnología de Rosario; ArgentinaFil: Osorio, Marina B.. Universidade Federal do Rio Grande do Sul; BrasilFil: Homrich, Milena S.. Universidade Federal do Rio Grande do Sul; BrasilFil: Turchetto Zolet, Andreia C.. Universidade Federal do Rio Grande do Sul; BrasilFil: De Carvalho, Mayra C.C.G.. Ministerio da Agricultura Pecuaria e Abastecimento de Brasil. Empresa Brasileira de Pesquisa Agropecuaria; BrasilFil: Stolf, Renata. Ministerio da Agricultura Pecuaria e Abastecimento de Brasil. Empresa Brasileira de Pesquisa Agropecuaria; BrasilFil: Weber, Ricardo L.M.. Universidade Federal do Rio Grande do Sul; BrasilFil: Westergaard, Gastón. Instituto de Agrobiotecnología de Rosario (indear); ArgentinaFil: Castagnaro, Atilio Pedro. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Instituto de Tecnología Agroindustrial del Noroeste Argentino. Provincia de Tucumán. Ministerio de Desarrollo Productivo. Estación Experimental Agroindustrial ; ArgentinaFil: Abdelnoor, Ricardo V.. Ministerio da Agricultura Pecuaria e Abastecimento de Brasil. Empresa Brasileira de Pesquisa Agropecuaria; BrasilFil: Marcelino Guimarães, Francismar C.. Ministerio da Agricultura Pecuaria e Abastecimento de Brasil. Empresa Brasileira de Pesquisa Agropecuaria; BrasilFil: Margis Pinheiro, Márcia. Universidade Federal do Rio Grande do Sul; BrasilFil: Bodanese Zanettini, Maria H.. Universidade Federal do Rio Grande do Sul; BrasilBioMed Central2014-09info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/36572Bencke Malato, Marta; Cabreira, Caroline; Wiebke Strohm, Beatriz; Bücker Neto, Lauro; Mancini, Estefania; et al.; Genome-wide annotation of the soybean WRKY family and functional characterization of genes involved in response to Phakopsora pachyrhizi infection; BioMed Central; BMC Plant Biology; 14; 1; 9-2014; 1-181471-2229CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://bmcplantbiol.biomedcentral.com/articles/10.1186/s12870-014-0236-0info:eu-repo/semantics/altIdentifier/doi/10.1186/s12870-014-0236-0info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-10-22T11:51:33Zoai:ri.conicet.gov.ar:11336/36572instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-10-22 11:51:33.766CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Genome-wide annotation of the soybean WRKY family and functional characterization of genes involved in response to Phakopsora pachyrhizi infection
title Genome-wide annotation of the soybean WRKY family and functional characterization of genes involved in response to Phakopsora pachyrhizi infection
spellingShingle Genome-wide annotation of the soybean WRKY family and functional characterization of genes involved in response to Phakopsora pachyrhizi infection
Bencke Malato, Marta
ASIAN SOYBEAN RUST
FUNCTIONAL ANALYSIS
FUNGUS RESISTANCE
GENETIC TRANSFORMATION
GLYCINE MAX
TRANSCRIPTION FACTORS
title_short Genome-wide annotation of the soybean WRKY family and functional characterization of genes involved in response to Phakopsora pachyrhizi infection
title_full Genome-wide annotation of the soybean WRKY family and functional characterization of genes involved in response to Phakopsora pachyrhizi infection
title_fullStr Genome-wide annotation of the soybean WRKY family and functional characterization of genes involved in response to Phakopsora pachyrhizi infection
title_full_unstemmed Genome-wide annotation of the soybean WRKY family and functional characterization of genes involved in response to Phakopsora pachyrhizi infection
title_sort Genome-wide annotation of the soybean WRKY family and functional characterization of genes involved in response to Phakopsora pachyrhizi infection
dc.creator.none.fl_str_mv Bencke Malato, Marta
Cabreira, Caroline
Wiebke Strohm, Beatriz
Bücker Neto, Lauro
Mancini, Estefania
Osorio, Marina B.
Homrich, Milena S.
Turchetto Zolet, Andreia C.
De Carvalho, Mayra C.C.G.
Stolf, Renata
Weber, Ricardo L.M.
Westergaard, Gastón
Castagnaro, Atilio Pedro
Abdelnoor, Ricardo V.
Marcelino Guimarães, Francismar C.
Margis Pinheiro, Márcia
Bodanese Zanettini, Maria H.
author Bencke Malato, Marta
author_facet Bencke Malato, Marta
Cabreira, Caroline
Wiebke Strohm, Beatriz
Bücker Neto, Lauro
Mancini, Estefania
Osorio, Marina B.
Homrich, Milena S.
Turchetto Zolet, Andreia C.
De Carvalho, Mayra C.C.G.
Stolf, Renata
Weber, Ricardo L.M.
Westergaard, Gastón
Castagnaro, Atilio Pedro
Abdelnoor, Ricardo V.
Marcelino Guimarães, Francismar C.
Margis Pinheiro, Márcia
Bodanese Zanettini, Maria H.
author_role author
author2 Cabreira, Caroline
Wiebke Strohm, Beatriz
Bücker Neto, Lauro
Mancini, Estefania
Osorio, Marina B.
Homrich, Milena S.
Turchetto Zolet, Andreia C.
De Carvalho, Mayra C.C.G.
Stolf, Renata
Weber, Ricardo L.M.
Westergaard, Gastón
Castagnaro, Atilio Pedro
Abdelnoor, Ricardo V.
Marcelino Guimarães, Francismar C.
Margis Pinheiro, Márcia
Bodanese Zanettini, Maria H.
author2_role author
author
author
author
author
author
author
author
author
author
author
author
author
author
author
author
dc.subject.none.fl_str_mv ASIAN SOYBEAN RUST
FUNCTIONAL ANALYSIS
FUNGUS RESISTANCE
GENETIC TRANSFORMATION
GLYCINE MAX
TRANSCRIPTION FACTORS
topic ASIAN SOYBEAN RUST
FUNCTIONAL ANALYSIS
FUNGUS RESISTANCE
GENETIC TRANSFORMATION
GLYCINE MAX
TRANSCRIPTION FACTORS
purl_subject.fl_str_mv https://purl.org/becyt/ford/4.4
https://purl.org/becyt/ford/4
dc.description.none.fl_txt_mv Background: Many previous studies have shown that soybean WRKY transcription factors are involved in the plant response to biotic and abiotic stresses. Phakopsora pachyrhizi is the causal agent of Asian Soybean Rust, one of the most important soybean diseases. There are evidences that WRKYs are involved in the resistance of some soybean genotypes against that fungus. The number of WRKY genes already annotated in soybean genome was underrepresented. In the present study, a genome-wide annotation of the soybean WRKY family was carried out and members involved in the response to P. pachyrhizi were identified.Results: As a result of a soybean genomic databases search, 182 WRKY-encoding genes were annotated and 33 putative pseudogenes identified. Genes involved in the response to P. pachyrhizi infection were identified using superSAGE, RNA-Seq of microdissected lesions and microarray experiments. Seventy-five genes were differentially expressed during fungal infection. The expression of eight WRKY genes was validated by RT-qPCR. The expression of these genes in a resistant genotype was earlier and/or stronger compared with a susceptible genotype in response to P. pachyrhizi infection. Soybean somatic embryos were transformed in order to overexpress or silence WRKY genes. Embryos overexpressing a WRKY gene were obtained, but they were unable to convert into plants. When infected with P. pachyrhizi, the leaves of the silenced transgenic line showed a higher number of lesions than the wild-type plants.Conclusions: The present study reports a genome-wide annotation of soybean WRKY family. The participation of some members in response to P. pachyrhizi infection was demonstrated. The results contribute to the elucidation of gene function and suggest the manipulation of WRKYs as a strategy to increase fungal resistance in soybean plants.
Fil: Bencke Malato, Marta. Universidade Federal do Rio Grande do Sul; Brasil
Fil: Cabreira, Caroline. Universidade Federal do Rio Grande do Sul; Brasil
Fil: Wiebke Strohm, Beatriz. Universidade Federal do Rio Grande do Sul; Brasil
Fil: Bücker Neto, Lauro. Universidade Federal do Rio Grande do Sul; Brasil
Fil: Mancini, Estefania. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Rosario. Instituto de Agrobiotecnología de Rosario; Argentina
Fil: Osorio, Marina B.. Universidade Federal do Rio Grande do Sul; Brasil
Fil: Homrich, Milena S.. Universidade Federal do Rio Grande do Sul; Brasil
Fil: Turchetto Zolet, Andreia C.. Universidade Federal do Rio Grande do Sul; Brasil
Fil: De Carvalho, Mayra C.C.G.. Ministerio da Agricultura Pecuaria e Abastecimento de Brasil. Empresa Brasileira de Pesquisa Agropecuaria; Brasil
Fil: Stolf, Renata. Ministerio da Agricultura Pecuaria e Abastecimento de Brasil. Empresa Brasileira de Pesquisa Agropecuaria; Brasil
Fil: Weber, Ricardo L.M.. Universidade Federal do Rio Grande do Sul; Brasil
Fil: Westergaard, Gastón. Instituto de Agrobiotecnología de Rosario (indear); Argentina
Fil: Castagnaro, Atilio Pedro. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Instituto de Tecnología Agroindustrial del Noroeste Argentino. Provincia de Tucumán. Ministerio de Desarrollo Productivo. Estación Experimental Agroindustrial ; Argentina
Fil: Abdelnoor, Ricardo V.. Ministerio da Agricultura Pecuaria e Abastecimento de Brasil. Empresa Brasileira de Pesquisa Agropecuaria; Brasil
Fil: Marcelino Guimarães, Francismar C.. Ministerio da Agricultura Pecuaria e Abastecimento de Brasil. Empresa Brasileira de Pesquisa Agropecuaria; Brasil
Fil: Margis Pinheiro, Márcia. Universidade Federal do Rio Grande do Sul; Brasil
Fil: Bodanese Zanettini, Maria H.. Universidade Federal do Rio Grande do Sul; Brasil
description Background: Many previous studies have shown that soybean WRKY transcription factors are involved in the plant response to biotic and abiotic stresses. Phakopsora pachyrhizi is the causal agent of Asian Soybean Rust, one of the most important soybean diseases. There are evidences that WRKYs are involved in the resistance of some soybean genotypes against that fungus. The number of WRKY genes already annotated in soybean genome was underrepresented. In the present study, a genome-wide annotation of the soybean WRKY family was carried out and members involved in the response to P. pachyrhizi were identified.Results: As a result of a soybean genomic databases search, 182 WRKY-encoding genes were annotated and 33 putative pseudogenes identified. Genes involved in the response to P. pachyrhizi infection were identified using superSAGE, RNA-Seq of microdissected lesions and microarray experiments. Seventy-five genes were differentially expressed during fungal infection. The expression of eight WRKY genes was validated by RT-qPCR. The expression of these genes in a resistant genotype was earlier and/or stronger compared with a susceptible genotype in response to P. pachyrhizi infection. Soybean somatic embryos were transformed in order to overexpress or silence WRKY genes. Embryos overexpressing a WRKY gene were obtained, but they were unable to convert into plants. When infected with P. pachyrhizi, the leaves of the silenced transgenic line showed a higher number of lesions than the wild-type plants.Conclusions: The present study reports a genome-wide annotation of soybean WRKY family. The participation of some members in response to P. pachyrhizi infection was demonstrated. The results contribute to the elucidation of gene function and suggest the manipulation of WRKYs as a strategy to increase fungal resistance in soybean plants.
publishDate 2014
dc.date.none.fl_str_mv 2014-09
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/36572
Bencke Malato, Marta; Cabreira, Caroline; Wiebke Strohm, Beatriz; Bücker Neto, Lauro; Mancini, Estefania; et al.; Genome-wide annotation of the soybean WRKY family and functional characterization of genes involved in response to Phakopsora pachyrhizi infection; BioMed Central; BMC Plant Biology; 14; 1; 9-2014; 1-18
1471-2229
CONICET Digital
CONICET
url http://hdl.handle.net/11336/36572
identifier_str_mv Bencke Malato, Marta; Cabreira, Caroline; Wiebke Strohm, Beatriz; Bücker Neto, Lauro; Mancini, Estefania; et al.; Genome-wide annotation of the soybean WRKY family and functional characterization of genes involved in response to Phakopsora pachyrhizi infection; BioMed Central; BMC Plant Biology; 14; 1; 9-2014; 1-18
1471-2229
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://bmcplantbiol.biomedcentral.com/articles/10.1186/s12870-014-0236-0
info:eu-repo/semantics/altIdentifier/doi/10.1186/s12870-014-0236-0
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv BioMed Central
publisher.none.fl_str_mv BioMed Central
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 12.982451

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