Genome-wide annotation of the soybean WRKY family and functional characterization of genes involved in response to Phakopsora pachyrhizi infection
- Autores
- Bencke Malato, Marta; Cabreira, Caroline; Wiebke Strohm, Beatriz; Bücker Neto, Lauro; Mancini, Estefania; Osorio, Marina B.; Homrich, Milena S.; Turchetto Zolet, Andreia C.; De Carvalho, Mayra C.C.G.; Stolf, Renata; Weber, Ricardo L.M.; Westergaard, Gastón; Castagnaro, Atilio Pedro; Abdelnoor, Ricardo V.; Marcelino Guimarães, Francismar C.; Margis Pinheiro, Márcia; Bodanese Zanettini, Maria H.
- Año de publicación
- 2014
- Idioma
- inglés
- Tipo de recurso
- artículo
- Estado
- versión publicada
- Descripción
- Background: Many previous studies have shown that soybean WRKY transcription factors are involved in the plant response to biotic and abiotic stresses. Phakopsora pachyrhizi is the causal agent of Asian Soybean Rust, one of the most important soybean diseases. There are evidences that WRKYs are involved in the resistance of some soybean genotypes against that fungus. The number of WRKY genes already annotated in soybean genome was underrepresented. In the present study, a genome-wide annotation of the soybean WRKY family was carried out and members involved in the response to P. pachyrhizi were identified.Results: As a result of a soybean genomic databases search, 182 WRKY-encoding genes were annotated and 33 putative pseudogenes identified. Genes involved in the response to P. pachyrhizi infection were identified using superSAGE, RNA-Seq of microdissected lesions and microarray experiments. Seventy-five genes were differentially expressed during fungal infection. The expression of eight WRKY genes was validated by RT-qPCR. The expression of these genes in a resistant genotype was earlier and/or stronger compared with a susceptible genotype in response to P. pachyrhizi infection. Soybean somatic embryos were transformed in order to overexpress or silence WRKY genes. Embryos overexpressing a WRKY gene were obtained, but they were unable to convert into plants. When infected with P. pachyrhizi, the leaves of the silenced transgenic line showed a higher number of lesions than the wild-type plants.Conclusions: The present study reports a genome-wide annotation of soybean WRKY family. The participation of some members in response to P. pachyrhizi infection was demonstrated. The results contribute to the elucidation of gene function and suggest the manipulation of WRKYs as a strategy to increase fungal resistance in soybean plants.
Fil: Bencke Malato, Marta. Universidade Federal do Rio Grande do Sul; Brasil
Fil: Cabreira, Caroline. Universidade Federal do Rio Grande do Sul; Brasil
Fil: Wiebke Strohm, Beatriz. Universidade Federal do Rio Grande do Sul; Brasil
Fil: Bücker Neto, Lauro. Universidade Federal do Rio Grande do Sul; Brasil
Fil: Mancini, Estefania. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Rosario. Instituto de Agrobiotecnología de Rosario; Argentina
Fil: Osorio, Marina B.. Universidade Federal do Rio Grande do Sul; Brasil
Fil: Homrich, Milena S.. Universidade Federal do Rio Grande do Sul; Brasil
Fil: Turchetto Zolet, Andreia C.. Universidade Federal do Rio Grande do Sul; Brasil
Fil: De Carvalho, Mayra C.C.G.. Ministerio da Agricultura Pecuaria e Abastecimento de Brasil. Empresa Brasileira de Pesquisa Agropecuaria; Brasil
Fil: Stolf, Renata. Ministerio da Agricultura Pecuaria e Abastecimento de Brasil. Empresa Brasileira de Pesquisa Agropecuaria; Brasil
Fil: Weber, Ricardo L.M.. Universidade Federal do Rio Grande do Sul; Brasil
Fil: Westergaard, Gastón. Instituto de Agrobiotecnología de Rosario (indear); Argentina
Fil: Castagnaro, Atilio Pedro. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Instituto de Tecnología Agroindustrial del Noroeste Argentino. Provincia de Tucumán. Ministerio de Desarrollo Productivo. Estación Experimental Agroindustrial ; Argentina
Fil: Abdelnoor, Ricardo V.. Ministerio da Agricultura Pecuaria e Abastecimento de Brasil. Empresa Brasileira de Pesquisa Agropecuaria; Brasil
Fil: Marcelino Guimarães, Francismar C.. Ministerio da Agricultura Pecuaria e Abastecimento de Brasil. Empresa Brasileira de Pesquisa Agropecuaria; Brasil
Fil: Margis Pinheiro, Márcia. Universidade Federal do Rio Grande do Sul; Brasil
Fil: Bodanese Zanettini, Maria H.. Universidade Federal do Rio Grande do Sul; Brasil - Materia
-
ASIAN SOYBEAN RUST
FUNCTIONAL ANALYSIS
FUNGUS RESISTANCE
GENETIC TRANSFORMATION
GLYCINE MAX
TRANSCRIPTION FACTORS - Nivel de accesibilidad
- acceso abierto
- Condiciones de uso
- https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
- Repositorio
- Institución
- Consejo Nacional de Investigaciones Científicas y Técnicas
- OAI Identificador
- oai:ri.conicet.gov.ar:11336/36572
Ver los metadatos del registro completo
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oai:ri.conicet.gov.ar:11336/36572 |
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CONICET Digital (CONICET) |
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Genome-wide annotation of the soybean WRKY family and functional characterization of genes involved in response to Phakopsora pachyrhizi infectionBencke Malato, MartaCabreira, CarolineWiebke Strohm, BeatrizBücker Neto, LauroMancini, EstefaniaOsorio, Marina B.Homrich, Milena S.Turchetto Zolet, Andreia C.De Carvalho, Mayra C.C.G.Stolf, RenataWeber, Ricardo L.M.Westergaard, GastónCastagnaro, Atilio PedroAbdelnoor, Ricardo V.Marcelino Guimarães, Francismar C.Margis Pinheiro, MárciaBodanese Zanettini, Maria H.ASIAN SOYBEAN RUSTFUNCTIONAL ANALYSISFUNGUS RESISTANCEGENETIC TRANSFORMATIONGLYCINE MAXTRANSCRIPTION FACTORShttps://purl.org/becyt/ford/4.4https://purl.org/becyt/ford/4Background: Many previous studies have shown that soybean WRKY transcription factors are involved in the plant response to biotic and abiotic stresses. Phakopsora pachyrhizi is the causal agent of Asian Soybean Rust, one of the most important soybean diseases. There are evidences that WRKYs are involved in the resistance of some soybean genotypes against that fungus. The number of WRKY genes already annotated in soybean genome was underrepresented. In the present study, a genome-wide annotation of the soybean WRKY family was carried out and members involved in the response to P. pachyrhizi were identified.Results: As a result of a soybean genomic databases search, 182 WRKY-encoding genes were annotated and 33 putative pseudogenes identified. Genes involved in the response to P. pachyrhizi infection were identified using superSAGE, RNA-Seq of microdissected lesions and microarray experiments. Seventy-five genes were differentially expressed during fungal infection. The expression of eight WRKY genes was validated by RT-qPCR. The expression of these genes in a resistant genotype was earlier and/or stronger compared with a susceptible genotype in response to P. pachyrhizi infection. Soybean somatic embryos were transformed in order to overexpress or silence WRKY genes. Embryos overexpressing a WRKY gene were obtained, but they were unable to convert into plants. When infected with P. pachyrhizi, the leaves of the silenced transgenic line showed a higher number of lesions than the wild-type plants.Conclusions: The present study reports a genome-wide annotation of soybean WRKY family. The participation of some members in response to P. pachyrhizi infection was demonstrated. The results contribute to the elucidation of gene function and suggest the manipulation of WRKYs as a strategy to increase fungal resistance in soybean plants.Fil: Bencke Malato, Marta. Universidade Federal do Rio Grande do Sul; BrasilFil: Cabreira, Caroline. Universidade Federal do Rio Grande do Sul; BrasilFil: Wiebke Strohm, Beatriz. Universidade Federal do Rio Grande do Sul; BrasilFil: Bücker Neto, Lauro. Universidade Federal do Rio Grande do Sul; BrasilFil: Mancini, Estefania. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Rosario. Instituto de Agrobiotecnología de Rosario; ArgentinaFil: Osorio, Marina B.. Universidade Federal do Rio Grande do Sul; BrasilFil: Homrich, Milena S.. Universidade Federal do Rio Grande do Sul; BrasilFil: Turchetto Zolet, Andreia C.. Universidade Federal do Rio Grande do Sul; BrasilFil: De Carvalho, Mayra C.C.G.. Ministerio da Agricultura Pecuaria e Abastecimento de Brasil. Empresa Brasileira de Pesquisa Agropecuaria; BrasilFil: Stolf, Renata. Ministerio da Agricultura Pecuaria e Abastecimento de Brasil. Empresa Brasileira de Pesquisa Agropecuaria; BrasilFil: Weber, Ricardo L.M.. Universidade Federal do Rio Grande do Sul; BrasilFil: Westergaard, Gastón. Instituto de Agrobiotecnología de Rosario (indear); ArgentinaFil: Castagnaro, Atilio Pedro. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Instituto de Tecnología Agroindustrial del Noroeste Argentino. Provincia de Tucumán. Ministerio de Desarrollo Productivo. Estación Experimental Agroindustrial ; ArgentinaFil: Abdelnoor, Ricardo V.. Ministerio da Agricultura Pecuaria e Abastecimento de Brasil. Empresa Brasileira de Pesquisa Agropecuaria; BrasilFil: Marcelino Guimarães, Francismar C.. Ministerio da Agricultura Pecuaria e Abastecimento de Brasil. Empresa Brasileira de Pesquisa Agropecuaria; BrasilFil: Margis Pinheiro, Márcia. Universidade Federal do Rio Grande do Sul; BrasilFil: Bodanese Zanettini, Maria H.. Universidade Federal do Rio Grande do Sul; BrasilBioMed Central2014-09info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/36572Bencke Malato, Marta; Cabreira, Caroline; Wiebke Strohm, Beatriz; Bücker Neto, Lauro; Mancini, Estefania; et al.; Genome-wide annotation of the soybean WRKY family and functional characterization of genes involved in response to Phakopsora pachyrhizi infection; BioMed Central; BMC Plant Biology; 14; 1; 9-2014; 1-181471-2229CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://bmcplantbiol.biomedcentral.com/articles/10.1186/s12870-014-0236-0info:eu-repo/semantics/altIdentifier/doi/10.1186/s12870-014-0236-0info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T10:18:49Zoai:ri.conicet.gov.ar:11336/36572instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 10:18:49.602CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse |
dc.title.none.fl_str_mv |
Genome-wide annotation of the soybean WRKY family and functional characterization of genes involved in response to Phakopsora pachyrhizi infection |
title |
Genome-wide annotation of the soybean WRKY family and functional characterization of genes involved in response to Phakopsora pachyrhizi infection |
spellingShingle |
Genome-wide annotation of the soybean WRKY family and functional characterization of genes involved in response to Phakopsora pachyrhizi infection Bencke Malato, Marta ASIAN SOYBEAN RUST FUNCTIONAL ANALYSIS FUNGUS RESISTANCE GENETIC TRANSFORMATION GLYCINE MAX TRANSCRIPTION FACTORS |
title_short |
Genome-wide annotation of the soybean WRKY family and functional characterization of genes involved in response to Phakopsora pachyrhizi infection |
title_full |
Genome-wide annotation of the soybean WRKY family and functional characterization of genes involved in response to Phakopsora pachyrhizi infection |
title_fullStr |
Genome-wide annotation of the soybean WRKY family and functional characterization of genes involved in response to Phakopsora pachyrhizi infection |
title_full_unstemmed |
Genome-wide annotation of the soybean WRKY family and functional characterization of genes involved in response to Phakopsora pachyrhizi infection |
title_sort |
Genome-wide annotation of the soybean WRKY family and functional characterization of genes involved in response to Phakopsora pachyrhizi infection |
dc.creator.none.fl_str_mv |
Bencke Malato, Marta Cabreira, Caroline Wiebke Strohm, Beatriz Bücker Neto, Lauro Mancini, Estefania Osorio, Marina B. Homrich, Milena S. Turchetto Zolet, Andreia C. De Carvalho, Mayra C.C.G. Stolf, Renata Weber, Ricardo L.M. Westergaard, Gastón Castagnaro, Atilio Pedro Abdelnoor, Ricardo V. Marcelino Guimarães, Francismar C. Margis Pinheiro, Márcia Bodanese Zanettini, Maria H. |
author |
Bencke Malato, Marta |
author_facet |
Bencke Malato, Marta Cabreira, Caroline Wiebke Strohm, Beatriz Bücker Neto, Lauro Mancini, Estefania Osorio, Marina B. Homrich, Milena S. Turchetto Zolet, Andreia C. De Carvalho, Mayra C.C.G. Stolf, Renata Weber, Ricardo L.M. Westergaard, Gastón Castagnaro, Atilio Pedro Abdelnoor, Ricardo V. Marcelino Guimarães, Francismar C. Margis Pinheiro, Márcia Bodanese Zanettini, Maria H. |
author_role |
author |
author2 |
Cabreira, Caroline Wiebke Strohm, Beatriz Bücker Neto, Lauro Mancini, Estefania Osorio, Marina B. Homrich, Milena S. Turchetto Zolet, Andreia C. De Carvalho, Mayra C.C.G. Stolf, Renata Weber, Ricardo L.M. Westergaard, Gastón Castagnaro, Atilio Pedro Abdelnoor, Ricardo V. Marcelino Guimarães, Francismar C. Margis Pinheiro, Márcia Bodanese Zanettini, Maria H. |
author2_role |
author author author author author author author author author author author author author author author author |
dc.subject.none.fl_str_mv |
ASIAN SOYBEAN RUST FUNCTIONAL ANALYSIS FUNGUS RESISTANCE GENETIC TRANSFORMATION GLYCINE MAX TRANSCRIPTION FACTORS |
topic |
ASIAN SOYBEAN RUST FUNCTIONAL ANALYSIS FUNGUS RESISTANCE GENETIC TRANSFORMATION GLYCINE MAX TRANSCRIPTION FACTORS |
purl_subject.fl_str_mv |
https://purl.org/becyt/ford/4.4 https://purl.org/becyt/ford/4 |
dc.description.none.fl_txt_mv |
Background: Many previous studies have shown that soybean WRKY transcription factors are involved in the plant response to biotic and abiotic stresses. Phakopsora pachyrhizi is the causal agent of Asian Soybean Rust, one of the most important soybean diseases. There are evidences that WRKYs are involved in the resistance of some soybean genotypes against that fungus. The number of WRKY genes already annotated in soybean genome was underrepresented. In the present study, a genome-wide annotation of the soybean WRKY family was carried out and members involved in the response to P. pachyrhizi were identified.Results: As a result of a soybean genomic databases search, 182 WRKY-encoding genes were annotated and 33 putative pseudogenes identified. Genes involved in the response to P. pachyrhizi infection were identified using superSAGE, RNA-Seq of microdissected lesions and microarray experiments. Seventy-five genes were differentially expressed during fungal infection. The expression of eight WRKY genes was validated by RT-qPCR. The expression of these genes in a resistant genotype was earlier and/or stronger compared with a susceptible genotype in response to P. pachyrhizi infection. Soybean somatic embryos were transformed in order to overexpress or silence WRKY genes. Embryos overexpressing a WRKY gene were obtained, but they were unable to convert into plants. When infected with P. pachyrhizi, the leaves of the silenced transgenic line showed a higher number of lesions than the wild-type plants.Conclusions: The present study reports a genome-wide annotation of soybean WRKY family. The participation of some members in response to P. pachyrhizi infection was demonstrated. The results contribute to the elucidation of gene function and suggest the manipulation of WRKYs as a strategy to increase fungal resistance in soybean plants. Fil: Bencke Malato, Marta. Universidade Federal do Rio Grande do Sul; Brasil Fil: Cabreira, Caroline. Universidade Federal do Rio Grande do Sul; Brasil Fil: Wiebke Strohm, Beatriz. Universidade Federal do Rio Grande do Sul; Brasil Fil: Bücker Neto, Lauro. Universidade Federal do Rio Grande do Sul; Brasil Fil: Mancini, Estefania. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Rosario. Instituto de Agrobiotecnología de Rosario; Argentina Fil: Osorio, Marina B.. Universidade Federal do Rio Grande do Sul; Brasil Fil: Homrich, Milena S.. Universidade Federal do Rio Grande do Sul; Brasil Fil: Turchetto Zolet, Andreia C.. Universidade Federal do Rio Grande do Sul; Brasil Fil: De Carvalho, Mayra C.C.G.. Ministerio da Agricultura Pecuaria e Abastecimento de Brasil. Empresa Brasileira de Pesquisa Agropecuaria; Brasil Fil: Stolf, Renata. Ministerio da Agricultura Pecuaria e Abastecimento de Brasil. Empresa Brasileira de Pesquisa Agropecuaria; Brasil Fil: Weber, Ricardo L.M.. Universidade Federal do Rio Grande do Sul; Brasil Fil: Westergaard, Gastón. Instituto de Agrobiotecnología de Rosario (indear); Argentina Fil: Castagnaro, Atilio Pedro. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Instituto de Tecnología Agroindustrial del Noroeste Argentino. Provincia de Tucumán. Ministerio de Desarrollo Productivo. Estación Experimental Agroindustrial ; Argentina Fil: Abdelnoor, Ricardo V.. Ministerio da Agricultura Pecuaria e Abastecimento de Brasil. Empresa Brasileira de Pesquisa Agropecuaria; Brasil Fil: Marcelino Guimarães, Francismar C.. Ministerio da Agricultura Pecuaria e Abastecimento de Brasil. Empresa Brasileira de Pesquisa Agropecuaria; Brasil Fil: Margis Pinheiro, Márcia. Universidade Federal do Rio Grande do Sul; Brasil Fil: Bodanese Zanettini, Maria H.. Universidade Federal do Rio Grande do Sul; Brasil |
description |
Background: Many previous studies have shown that soybean WRKY transcription factors are involved in the plant response to biotic and abiotic stresses. Phakopsora pachyrhizi is the causal agent of Asian Soybean Rust, one of the most important soybean diseases. There are evidences that WRKYs are involved in the resistance of some soybean genotypes against that fungus. The number of WRKY genes already annotated in soybean genome was underrepresented. In the present study, a genome-wide annotation of the soybean WRKY family was carried out and members involved in the response to P. pachyrhizi were identified.Results: As a result of a soybean genomic databases search, 182 WRKY-encoding genes were annotated and 33 putative pseudogenes identified. Genes involved in the response to P. pachyrhizi infection were identified using superSAGE, RNA-Seq of microdissected lesions and microarray experiments. Seventy-five genes were differentially expressed during fungal infection. The expression of eight WRKY genes was validated by RT-qPCR. The expression of these genes in a resistant genotype was earlier and/or stronger compared with a susceptible genotype in response to P. pachyrhizi infection. Soybean somatic embryos were transformed in order to overexpress or silence WRKY genes. Embryos overexpressing a WRKY gene were obtained, but they were unable to convert into plants. When infected with P. pachyrhizi, the leaves of the silenced transgenic line showed a higher number of lesions than the wild-type plants.Conclusions: The present study reports a genome-wide annotation of soybean WRKY family. The participation of some members in response to P. pachyrhizi infection was demonstrated. The results contribute to the elucidation of gene function and suggest the manipulation of WRKYs as a strategy to increase fungal resistance in soybean plants. |
publishDate |
2014 |
dc.date.none.fl_str_mv |
2014-09 |
dc.type.none.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion http://purl.org/coar/resource_type/c_6501 info:ar-repo/semantics/articulo |
format |
article |
status_str |
publishedVersion |
dc.identifier.none.fl_str_mv |
http://hdl.handle.net/11336/36572 Bencke Malato, Marta; Cabreira, Caroline; Wiebke Strohm, Beatriz; Bücker Neto, Lauro; Mancini, Estefania; et al.; Genome-wide annotation of the soybean WRKY family and functional characterization of genes involved in response to Phakopsora pachyrhizi infection; BioMed Central; BMC Plant Biology; 14; 1; 9-2014; 1-18 1471-2229 CONICET Digital CONICET |
url |
http://hdl.handle.net/11336/36572 |
identifier_str_mv |
Bencke Malato, Marta; Cabreira, Caroline; Wiebke Strohm, Beatriz; Bücker Neto, Lauro; Mancini, Estefania; et al.; Genome-wide annotation of the soybean WRKY family and functional characterization of genes involved in response to Phakopsora pachyrhizi infection; BioMed Central; BMC Plant Biology; 14; 1; 9-2014; 1-18 1471-2229 CONICET Digital CONICET |
dc.language.none.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
info:eu-repo/semantics/altIdentifier/url/https://bmcplantbiol.biomedcentral.com/articles/10.1186/s12870-014-0236-0 info:eu-repo/semantics/altIdentifier/doi/10.1186/s12870-014-0236-0 |
dc.rights.none.fl_str_mv |
info:eu-repo/semantics/openAccess https://creativecommons.org/licenses/by-nc-sa/2.5/ar/ |
eu_rights_str_mv |
openAccess |
rights_invalid_str_mv |
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/ |
dc.format.none.fl_str_mv |
application/pdf application/pdf application/pdf |
dc.publisher.none.fl_str_mv |
BioMed Central |
publisher.none.fl_str_mv |
BioMed Central |
dc.source.none.fl_str_mv |
reponame:CONICET Digital (CONICET) instname:Consejo Nacional de Investigaciones Científicas y Técnicas |
reponame_str |
CONICET Digital (CONICET) |
collection |
CONICET Digital (CONICET) |
instname_str |
Consejo Nacional de Investigaciones Científicas y Técnicas |
repository.name.fl_str_mv |
CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas |
repository.mail.fl_str_mv |
dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar |
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1844614154111942656 |
score |
13.070432 |