Detection of bovine IgG isotypes in a PPA-ELISA for Johne's disease diagnosis in infected herds

Autores
Fernández, Bárbara; Gilardoni, Liliana Rosa; Jolly, Ana; Colavecchia, Silvia Beatriz; Paolicchi, Fernando Alberto; Mundo, Silvia Leonor
Año de publicación
2012
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Johne’s Disease or Paratuberculosis is a chronic granulomatous enteritis disease affecting ruminants. Detection of subclinically infected animals is difficult, hampering the control of this disease. The aim of this work was to evaluate the performance of detection of IgG isotypes in a PPA-ELISA to improve the recognition of cattle naturally infected with Map in different stages. A total of 108 animals from Tuberculosis-free herds were grouped as follows: exposed (n = 30), subclinically infected (n = 26), clinically infected (n = 14), and healthy controls (n = 38). Receiver-operating characteristic (ROC) curves of isotypes/PPA-ELISAs were constructed and areas under the curves were compared to evaluate the performance of each test. Our study demonstrated that the conventional PPA-ELISA (detecting IgG) is the best to identify clinically infected animals with high sensitivity (92.9%) and specificity (100%). Meanwhile, IgG2/PPA-ELISA improved the number of subclinically infected cattle detected as compared with conventional IgG/PPA-ELISA (53.8 versus 23.1%). In addition, it had the maximum sensitivity (65.0%, taking into account all Map-infected cattle). In conclusion, the combination of IgG and IgG2/PPA-ELISAs may improve the identification of Mapinfected cattle in different stages of disease. The usefulness of IgG2 detection in serological tests for Johne’s Disease diagnosis should be further evaluated.
Fil: Fernández, Bárbara. Universidad de Buenos Aires. Facultad de Ciencias Veterinarias. Cátedra de Inmunología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Gilardoni, Liliana Rosa. Universidad de Buenos Aires. Facultad de Ciencias Veterinarias. Cátedra de Inmunología; Argentina
Fil: Jolly, Ana. Universidad de Buenos Aires. Facultad de Ciencias Veterinarias. Cátedra de Inmunología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Colavecchia, Silvia Beatriz. Universidad de Buenos Aires. Facultad de Ciencias Veterinarias. Cátedra de Inmunología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Paolicchi, Fernando Alberto. Instituto Nacional de Tecnología Agropecuaria. Centro Regional Buenos Aires Sur. Estación Experimental Agropecuaria Balcarce. Agencia de Extensión Rural Balcarce; Argentina. Universidad Nacional de Mar del Plata. Facultad de Cs.agrarias. Departamento de Producción Animal; Argentina
Fil: Mundo, Silvia Leonor. Universidad de Buenos Aires. Facultad de Ciencias Veterinarias. Cátedra de Inmunología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Materia
PARATUBERCULOSIS
BOVINE
ANTIBODIES
ISOTYPES
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/137214
Acceder
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network_name_str CONICET Digital (CONICET)
spelling Detection of bovine IgG isotypes in a PPA-ELISA for Johne's disease diagnosis in infected herdsFernández, BárbaraGilardoni, Liliana RosaJolly, AnaColavecchia, Silvia BeatrizPaolicchi, Fernando AlbertoMundo, Silvia LeonorPARATUBERCULOSISBOVINEANTIBODIESISOTYPEShttps://purl.org/becyt/ford/4.3https://purl.org/becyt/ford/4Johne’s Disease or Paratuberculosis is a chronic granulomatous enteritis disease affecting ruminants. Detection of subclinically infected animals is difficult, hampering the control of this disease. The aim of this work was to evaluate the performance of detection of IgG isotypes in a PPA-ELISA to improve the recognition of cattle naturally infected with Map in different stages. A total of 108 animals from Tuberculosis-free herds were grouped as follows: exposed (n = 30), subclinically infected (n = 26), clinically infected (n = 14), and healthy controls (n = 38). Receiver-operating characteristic (ROC) curves of isotypes/PPA-ELISAs were constructed and areas under the curves were compared to evaluate the performance of each test. Our study demonstrated that the conventional PPA-ELISA (detecting IgG) is the best to identify clinically infected animals with high sensitivity (92.9%) and specificity (100%). Meanwhile, IgG2/PPA-ELISA improved the number of subclinically infected cattle detected as compared with conventional IgG/PPA-ELISA (53.8 versus 23.1%). In addition, it had the maximum sensitivity (65.0%, taking into account all Map-infected cattle). In conclusion, the combination of IgG and IgG2/PPA-ELISAs may improve the identification of Mapinfected cattle in different stages of disease. The usefulness of IgG2 detection in serological tests for Johne’s Disease diagnosis should be further evaluated.Fil: Fernández, Bárbara. Universidad de Buenos Aires. Facultad de Ciencias Veterinarias. Cátedra de Inmunología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Gilardoni, Liliana Rosa. Universidad de Buenos Aires. Facultad de Ciencias Veterinarias. Cátedra de Inmunología; ArgentinaFil: Jolly, Ana. Universidad de Buenos Aires. Facultad de Ciencias Veterinarias. Cátedra de Inmunología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Colavecchia, Silvia Beatriz. Universidad de Buenos Aires. Facultad de Ciencias Veterinarias. Cátedra de Inmunología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Paolicchi, Fernando Alberto. Instituto Nacional de Tecnología Agropecuaria. Centro Regional Buenos Aires Sur. Estación Experimental Agropecuaria Balcarce. Agencia de Extensión Rural Balcarce; Argentina. Universidad Nacional de Mar del Plata. Facultad de Cs.agrarias. Departamento de Producción Animal; ArgentinaFil: Mundo, Silvia Leonor. Universidad de Buenos Aires. Facultad de Ciencias Veterinarias. Cátedra de Inmunología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaHindawi Limited2012-06-26info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/137214Fernández, Bárbara; Gilardoni, Liliana Rosa; Jolly, Ana; Colavecchia, Silvia Beatriz; Paolicchi, Fernando Alberto; et al.; Detection of bovine IgG isotypes in a PPA-ELISA for Johne's disease diagnosis in infected herds; Hindawi Limited; Veterinary Medicine International; 2012; 26-6-2012; 1-62042-00482090-8113CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.1155/2012/145318info:eu-repo/semantics/altIdentifier/url/https://www.hindawi.com/journals/vmi/2012/145318/info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-10T13:06:05Zoai:ri.conicet.gov.ar:11336/137214instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-10 13:06:05.831CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Detection of bovine IgG isotypes in a PPA-ELISA for Johne's disease diagnosis in infected herds
title Detection of bovine IgG isotypes in a PPA-ELISA for Johne's disease diagnosis in infected herds
spellingShingle Detection of bovine IgG isotypes in a PPA-ELISA for Johne's disease diagnosis in infected herds
Fernández, Bárbara
PARATUBERCULOSIS
BOVINE
ANTIBODIES
ISOTYPES
title_short Detection of bovine IgG isotypes in a PPA-ELISA for Johne's disease diagnosis in infected herds
title_full Detection of bovine IgG isotypes in a PPA-ELISA for Johne's disease diagnosis in infected herds
title_fullStr Detection of bovine IgG isotypes in a PPA-ELISA for Johne's disease diagnosis in infected herds
title_full_unstemmed Detection of bovine IgG isotypes in a PPA-ELISA for Johne's disease diagnosis in infected herds
title_sort Detection of bovine IgG isotypes in a PPA-ELISA for Johne's disease diagnosis in infected herds
dc.creator.none.fl_str_mv Fernández, Bárbara
Gilardoni, Liliana Rosa
Jolly, Ana
Colavecchia, Silvia Beatriz
Paolicchi, Fernando Alberto
Mundo, Silvia Leonor
author Fernández, Bárbara
author_facet Fernández, Bárbara
Gilardoni, Liliana Rosa
Jolly, Ana
Colavecchia, Silvia Beatriz
Paolicchi, Fernando Alberto
Mundo, Silvia Leonor
author_role author
author2 Gilardoni, Liliana Rosa
Jolly, Ana
Colavecchia, Silvia Beatriz
Paolicchi, Fernando Alberto
Mundo, Silvia Leonor
author2_role author
author
author
author
author
dc.subject.none.fl_str_mv PARATUBERCULOSIS
BOVINE
ANTIBODIES
ISOTYPES
topic PARATUBERCULOSIS
BOVINE
ANTIBODIES
ISOTYPES
purl_subject.fl_str_mv https://purl.org/becyt/ford/4.3
https://purl.org/becyt/ford/4
dc.description.none.fl_txt_mv Johne’s Disease or Paratuberculosis is a chronic granulomatous enteritis disease affecting ruminants. Detection of subclinically infected animals is difficult, hampering the control of this disease. The aim of this work was to evaluate the performance of detection of IgG isotypes in a PPA-ELISA to improve the recognition of cattle naturally infected with Map in different stages. A total of 108 animals from Tuberculosis-free herds were grouped as follows: exposed (n = 30), subclinically infected (n = 26), clinically infected (n = 14), and healthy controls (n = 38). Receiver-operating characteristic (ROC) curves of isotypes/PPA-ELISAs were constructed and areas under the curves were compared to evaluate the performance of each test. Our study demonstrated that the conventional PPA-ELISA (detecting IgG) is the best to identify clinically infected animals with high sensitivity (92.9%) and specificity (100%). Meanwhile, IgG2/PPA-ELISA improved the number of subclinically infected cattle detected as compared with conventional IgG/PPA-ELISA (53.8 versus 23.1%). In addition, it had the maximum sensitivity (65.0%, taking into account all Map-infected cattle). In conclusion, the combination of IgG and IgG2/PPA-ELISAs may improve the identification of Mapinfected cattle in different stages of disease. The usefulness of IgG2 detection in serological tests for Johne’s Disease diagnosis should be further evaluated.
Fil: Fernández, Bárbara. Universidad de Buenos Aires. Facultad de Ciencias Veterinarias. Cátedra de Inmunología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Gilardoni, Liliana Rosa. Universidad de Buenos Aires. Facultad de Ciencias Veterinarias. Cátedra de Inmunología; Argentina
Fil: Jolly, Ana. Universidad de Buenos Aires. Facultad de Ciencias Veterinarias. Cátedra de Inmunología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Colavecchia, Silvia Beatriz. Universidad de Buenos Aires. Facultad de Ciencias Veterinarias. Cátedra de Inmunología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Paolicchi, Fernando Alberto. Instituto Nacional de Tecnología Agropecuaria. Centro Regional Buenos Aires Sur. Estación Experimental Agropecuaria Balcarce. Agencia de Extensión Rural Balcarce; Argentina. Universidad Nacional de Mar del Plata. Facultad de Cs.agrarias. Departamento de Producción Animal; Argentina
Fil: Mundo, Silvia Leonor. Universidad de Buenos Aires. Facultad de Ciencias Veterinarias. Cátedra de Inmunología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
description Johne’s Disease or Paratuberculosis is a chronic granulomatous enteritis disease affecting ruminants. Detection of subclinically infected animals is difficult, hampering the control of this disease. The aim of this work was to evaluate the performance of detection of IgG isotypes in a PPA-ELISA to improve the recognition of cattle naturally infected with Map in different stages. A total of 108 animals from Tuberculosis-free herds were grouped as follows: exposed (n = 30), subclinically infected (n = 26), clinically infected (n = 14), and healthy controls (n = 38). Receiver-operating characteristic (ROC) curves of isotypes/PPA-ELISAs were constructed and areas under the curves were compared to evaluate the performance of each test. Our study demonstrated that the conventional PPA-ELISA (detecting IgG) is the best to identify clinically infected animals with high sensitivity (92.9%) and specificity (100%). Meanwhile, IgG2/PPA-ELISA improved the number of subclinically infected cattle detected as compared with conventional IgG/PPA-ELISA (53.8 versus 23.1%). In addition, it had the maximum sensitivity (65.0%, taking into account all Map-infected cattle). In conclusion, the combination of IgG and IgG2/PPA-ELISAs may improve the identification of Mapinfected cattle in different stages of disease. The usefulness of IgG2 detection in serological tests for Johne’s Disease diagnosis should be further evaluated.
publishDate 2012
dc.date.none.fl_str_mv 2012-06-26
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/137214
Fernández, Bárbara; Gilardoni, Liliana Rosa; Jolly, Ana; Colavecchia, Silvia Beatriz; Paolicchi, Fernando Alberto; et al.; Detection of bovine IgG isotypes in a PPA-ELISA for Johne's disease diagnosis in infected herds; Hindawi Limited; Veterinary Medicine International; 2012; 26-6-2012; 1-6
2042-0048
2090-8113
CONICET Digital
CONICET
url http://hdl.handle.net/11336/137214
identifier_str_mv Fernández, Bárbara; Gilardoni, Liliana Rosa; Jolly, Ana; Colavecchia, Silvia Beatriz; Paolicchi, Fernando Alberto; et al.; Detection of bovine IgG isotypes in a PPA-ELISA for Johne's disease diagnosis in infected herds; Hindawi Limited; Veterinary Medicine International; 2012; 26-6-2012; 1-6
2042-0048
2090-8113
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.1155/2012/145318
info:eu-repo/semantics/altIdentifier/url/https://www.hindawi.com/journals/vmi/2012/145318/
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Hindawi Limited
publisher.none.fl_str_mv Hindawi Limited
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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