Effect of cooling rate on sperm quality of cryopreserved Andalusian donkey spermatozoa

Autores
Demyda-Peyrás, Sebastian; Bottrel, M.; Acha, D.; Ortiz, I.; Hidalgo, M.; Carrasco, J.J.; Gómez Arrones, V.; Gósalvez, J.; Dorado, J.
Año de publicación
2018
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
The aim of this study was to evaluate the effect of different cooling rates on post-thaw quality of cryopreserved donkey spermatozoa. Eighteen ejaculates from six adult Andalusian donkeys (three ejaculates per donkey) were collected using an artificial vagina. Pooled semen samples (two ejaculates per pool) were divided into three aliquots, and frozen in Gent freezing extender using three different cryopreservation protocols (P): P1 (conventional slow freezing, as control): semen pre-cooled in an Equitainer for 2 h and frozen in liquid nitrogen (LN2) vapour; P2 (controlled pre-freeze cooling rate): semen pre-cooled at a controlled rate for 73 min and frozen in LN2 vapour; and P3 (rapid freezing) semen frozen immediately in LN2 vapour. After thawing at 37 °C for 30 s, semen samples were assessed for motility, morphology, acrosome and plasma membrane integrity; spermatozoa were also tested for DNA integrity. Significant (P < 0.01) differences were found between the cryopreservation protocols for all sperm parameters evaluated, except for DNA integrity. Semen samples frozen using P2 showed significantly (P < 0.01) higher values for sperm motility, morphology, sperm membrane integrity, and acrosome integrity. On the contrary, P3 reduced sperm motility (P < 0.01) and increased the percentage of spermatozoa with damaged plasma membrane (P < 0.001). In our study, we demonstrated that the sperm of Andalusian donkey is particularly sensitive to the cooling rate used before freezing. Furthermore, Andalusian donkey semen can be successfully cryopreserved using controlled cooling rates combined with freezing in LN2 vapour.
Fil: Demyda-Peyrás, Sebastian. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico CONICET- La Plata. Instituto de Genética Veterinaria "Ing. Fernando Noel Dulout". Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias. Instituto de Genética Veterinaria; Argentina. Universidad de Córdoba; España
Fil: Bottrel, M.. Universidad de Córdoba; España
Fil: Acha, D.. Universidad de Córdoba; España
Fil: Ortiz, I.. Universidad de Córdoba; España
Fil: Hidalgo, M.. Universidad de Córdoba; España
Fil: Carrasco, J.J.. Centro de Selección y Reproducción Animal; España
Fil: Gómez Arrones, V.. Centro de Selección y Reproducción Animal; España
Fil: Gósalvez, J.. Universidad Autónoma de Madrid; España
Fil: Dorado, J.. Universidad de Córdoba; España
Materia
Cooling Rate
Cryopreservation
Donkey
Equus Asinus
Spermatozoa
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/82746
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/82746
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Effect of cooling rate on sperm quality of cryopreserved Andalusian donkey spermatozoaDemyda-Peyrás, SebastianBottrel, M.Acha, D.Ortiz, I.Hidalgo, M.Carrasco, J.J.Gómez Arrones, V.Gósalvez, J.Dorado, J.Cooling RateCryopreservationDonkeyEquus AsinusSpermatozoahttps://purl.org/becyt/ford/4.4https://purl.org/becyt/ford/4The aim of this study was to evaluate the effect of different cooling rates on post-thaw quality of cryopreserved donkey spermatozoa. Eighteen ejaculates from six adult Andalusian donkeys (three ejaculates per donkey) were collected using an artificial vagina. Pooled semen samples (two ejaculates per pool) were divided into three aliquots, and frozen in Gent freezing extender using three different cryopreservation protocols (P): P1 (conventional slow freezing, as control): semen pre-cooled in an Equitainer for 2 h and frozen in liquid nitrogen (LN2) vapour; P2 (controlled pre-freeze cooling rate): semen pre-cooled at a controlled rate for 73 min and frozen in LN2 vapour; and P3 (rapid freezing) semen frozen immediately in LN2 vapour. After thawing at 37 °C for 30 s, semen samples were assessed for motility, morphology, acrosome and plasma membrane integrity; spermatozoa were also tested for DNA integrity. Significant (P < 0.01) differences were found between the cryopreservation protocols for all sperm parameters evaluated, except for DNA integrity. Semen samples frozen using P2 showed significantly (P < 0.01) higher values for sperm motility, morphology, sperm membrane integrity, and acrosome integrity. On the contrary, P3 reduced sperm motility (P < 0.01) and increased the percentage of spermatozoa with damaged plasma membrane (P < 0.001). In our study, we demonstrated that the sperm of Andalusian donkey is particularly sensitive to the cooling rate used before freezing. Furthermore, Andalusian donkey semen can be successfully cryopreserved using controlled cooling rates combined with freezing in LN2 vapour.Fil: Demyda-Peyrás, Sebastian. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico CONICET- La Plata. Instituto de Genética Veterinaria "Ing. Fernando Noel Dulout". Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias. Instituto de Genética Veterinaria; Argentina. Universidad de Córdoba; EspañaFil: Bottrel, M.. Universidad de Córdoba; EspañaFil: Acha, D.. Universidad de Córdoba; EspañaFil: Ortiz, I.. Universidad de Córdoba; EspañaFil: Hidalgo, M.. Universidad de Córdoba; EspañaFil: Carrasco, J.J.. Centro de Selección y Reproducción Animal; EspañaFil: Gómez Arrones, V.. Centro de Selección y Reproducción Animal; EspañaFil: Gósalvez, J.. Universidad Autónoma de Madrid; EspañaFil: Dorado, J.. Universidad de Córdoba; EspañaElsevier Science2018-06info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/82746Demyda-Peyrás, Sebastian; Bottrel, M.; Acha, D.; Ortiz, I.; Hidalgo, M.; et al.; Effect of cooling rate on sperm quality of cryopreserved Andalusian donkey spermatozoa; Elsevier Science; Animal Reproduction Science; 193; 6-2018; 201-2080378-4320CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/pii/S0378432018300940info:eu-repo/semantics/altIdentifier/doi/10.1016/j.anireprosci.2018.04.069info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-nd/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-10-22T11:02:45Zoai:ri.conicet.gov.ar:11336/82746instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-10-22 11:02:45.433CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Effect of cooling rate on sperm quality of cryopreserved Andalusian donkey spermatozoa
title Effect of cooling rate on sperm quality of cryopreserved Andalusian donkey spermatozoa
spellingShingle Effect of cooling rate on sperm quality of cryopreserved Andalusian donkey spermatozoa
Demyda-Peyrás, Sebastian
Cooling Rate
Cryopreservation
Donkey
Equus Asinus
Spermatozoa
title_short Effect of cooling rate on sperm quality of cryopreserved Andalusian donkey spermatozoa
title_full Effect of cooling rate on sperm quality of cryopreserved Andalusian donkey spermatozoa
title_fullStr Effect of cooling rate on sperm quality of cryopreserved Andalusian donkey spermatozoa
title_full_unstemmed Effect of cooling rate on sperm quality of cryopreserved Andalusian donkey spermatozoa
title_sort Effect of cooling rate on sperm quality of cryopreserved Andalusian donkey spermatozoa
dc.creator.none.fl_str_mv Demyda-Peyrás, Sebastian
Bottrel, M.
Acha, D.
Ortiz, I.
Hidalgo, M.
Carrasco, J.J.
Gómez Arrones, V.
Gósalvez, J.
Dorado, J.
author Demyda-Peyrás, Sebastian
author_facet Demyda-Peyrás, Sebastian
Bottrel, M.
Acha, D.
Ortiz, I.
Hidalgo, M.
Carrasco, J.J.
Gómez Arrones, V.
Gósalvez, J.
Dorado, J.
author_role author
author2 Bottrel, M.
Acha, D.
Ortiz, I.
Hidalgo, M.
Carrasco, J.J.
Gómez Arrones, V.
Gósalvez, J.
Dorado, J.
author2_role author
author
author
author
author
author
author
author
dc.subject.none.fl_str_mv Cooling Rate
Cryopreservation
Donkey
Equus Asinus
Spermatozoa
topic Cooling Rate
Cryopreservation
Donkey
Equus Asinus
Spermatozoa
purl_subject.fl_str_mv https://purl.org/becyt/ford/4.4
https://purl.org/becyt/ford/4
dc.description.none.fl_txt_mv The aim of this study was to evaluate the effect of different cooling rates on post-thaw quality of cryopreserved donkey spermatozoa. Eighteen ejaculates from six adult Andalusian donkeys (three ejaculates per donkey) were collected using an artificial vagina. Pooled semen samples (two ejaculates per pool) were divided into three aliquots, and frozen in Gent freezing extender using three different cryopreservation protocols (P): P1 (conventional slow freezing, as control): semen pre-cooled in an Equitainer for 2 h and frozen in liquid nitrogen (LN2) vapour; P2 (controlled pre-freeze cooling rate): semen pre-cooled at a controlled rate for 73 min and frozen in LN2 vapour; and P3 (rapid freezing) semen frozen immediately in LN2 vapour. After thawing at 37 °C for 30 s, semen samples were assessed for motility, morphology, acrosome and plasma membrane integrity; spermatozoa were also tested for DNA integrity. Significant (P < 0.01) differences were found between the cryopreservation protocols for all sperm parameters evaluated, except for DNA integrity. Semen samples frozen using P2 showed significantly (P < 0.01) higher values for sperm motility, morphology, sperm membrane integrity, and acrosome integrity. On the contrary, P3 reduced sperm motility (P < 0.01) and increased the percentage of spermatozoa with damaged plasma membrane (P < 0.001). In our study, we demonstrated that the sperm of Andalusian donkey is particularly sensitive to the cooling rate used before freezing. Furthermore, Andalusian donkey semen can be successfully cryopreserved using controlled cooling rates combined with freezing in LN2 vapour.
Fil: Demyda-Peyrás, Sebastian. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico CONICET- La Plata. Instituto de Genética Veterinaria "Ing. Fernando Noel Dulout". Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias. Instituto de Genética Veterinaria; Argentina. Universidad de Córdoba; España
Fil: Bottrel, M.. Universidad de Córdoba; España
Fil: Acha, D.. Universidad de Córdoba; España
Fil: Ortiz, I.. Universidad de Córdoba; España
Fil: Hidalgo, M.. Universidad de Córdoba; España
Fil: Carrasco, J.J.. Centro de Selección y Reproducción Animal; España
Fil: Gómez Arrones, V.. Centro de Selección y Reproducción Animal; España
Fil: Gósalvez, J.. Universidad Autónoma de Madrid; España
Fil: Dorado, J.. Universidad de Córdoba; España
description The aim of this study was to evaluate the effect of different cooling rates on post-thaw quality of cryopreserved donkey spermatozoa. Eighteen ejaculates from six adult Andalusian donkeys (three ejaculates per donkey) were collected using an artificial vagina. Pooled semen samples (two ejaculates per pool) were divided into three aliquots, and frozen in Gent freezing extender using three different cryopreservation protocols (P): P1 (conventional slow freezing, as control): semen pre-cooled in an Equitainer for 2 h and frozen in liquid nitrogen (LN2) vapour; P2 (controlled pre-freeze cooling rate): semen pre-cooled at a controlled rate for 73 min and frozen in LN2 vapour; and P3 (rapid freezing) semen frozen immediately in LN2 vapour. After thawing at 37 °C for 30 s, semen samples were assessed for motility, morphology, acrosome and plasma membrane integrity; spermatozoa were also tested for DNA integrity. Significant (P < 0.01) differences were found between the cryopreservation protocols for all sperm parameters evaluated, except for DNA integrity. Semen samples frozen using P2 showed significantly (P < 0.01) higher values for sperm motility, morphology, sperm membrane integrity, and acrosome integrity. On the contrary, P3 reduced sperm motility (P < 0.01) and increased the percentage of spermatozoa with damaged plasma membrane (P < 0.001). In our study, we demonstrated that the sperm of Andalusian donkey is particularly sensitive to the cooling rate used before freezing. Furthermore, Andalusian donkey semen can be successfully cryopreserved using controlled cooling rates combined with freezing in LN2 vapour.
publishDate 2018
dc.date.none.fl_str_mv 2018-06
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/82746
Demyda-Peyrás, Sebastian; Bottrel, M.; Acha, D.; Ortiz, I.; Hidalgo, M.; et al.; Effect of cooling rate on sperm quality of cryopreserved Andalusian donkey spermatozoa; Elsevier Science; Animal Reproduction Science; 193; 6-2018; 201-208
0378-4320
CONICET Digital
CONICET
url http://hdl.handle.net/11336/82746
identifier_str_mv Demyda-Peyrás, Sebastian; Bottrel, M.; Acha, D.; Ortiz, I.; Hidalgo, M.; et al.; Effect of cooling rate on sperm quality of cryopreserved Andalusian donkey spermatozoa; Elsevier Science; Animal Reproduction Science; 193; 6-2018; 201-208
0378-4320
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/pii/S0378432018300940
info:eu-repo/semantics/altIdentifier/doi/10.1016/j.anireprosci.2018.04.069
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Elsevier Science
publisher.none.fl_str_mv Elsevier Science
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 12.982451

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