A bHLH-type transcription factor, JA-ASSOCIATED MYC2-LIKE1, acts as repressor to negatively regulate jasmonate signaling in Arabidopsis

Autores
Nakata, Masaru; Mitsuda, Nobutaka; Herde, Marco; Koo, Abraham J. K.; Moreno, Javier Edgardo; Suzuki, Kaoru; Howe, Gregg A.; Ohme Takagi, Masaru
Año de publicación
2013
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Jasmonates (JAs) are plant hormones that regulate the balance between plant growth and responses to biotic and abiotic stresses. Although recent studies have uncovered the mechanisms for JA-induced responses in Arabidopsis thaliana, the mechanisms by which plants attenuate the JA-induced responses remain elusive. Here, we report that a basic helix-loop-helix-type transcription factor, ABA-INDUCIBLE BHLH-TYPE TRANSCRIPTION FACTOR/JA-ASSOCIATED MYC2-LIKE1 (JAM1), acts as a transcriptional repressor and negatively regulates JA signaling. Gain-of-function transgenic plants expressing the chimeric repressor for JAM1 exhibited substantial reduction of JA responses, including JA-induced inhibition of root growth, accumulation of anthocyanin, and male fertility. These plants were also compromised in resistance to attack by the insect herbivore Spodoptera exigua. Conversely, jam1 loss-of-function mutants showed enhanced JA responsiveness, including increased resistance to insect attack. JAM1 and MYC2 competitively bind to the target sequence of MYC2, which likely provides the mechanism for negative regulation of JA signaling and suppression of MYC2 functions by JAM1. These results indicate that JAM1 negatively regulates JA signaling, thereby playing a pivotal role in fine-tuning of JA-mediated stress responses and plant growth.
Fil: Nakata, Masaru. National Institute of Advanced Industrial Science and Technology. Bioproduction Research Institute; Japón
Fil: Mitsuda, Nobutaka. National Institute of Advanced Industrial Science and Technology. Bioproduction Research Institute; Japón
Fil: Herde, Marco. Michigan State University; Estados Unidos
Fil: Koo, Abraham J. K.. Michigan State University; Estados Unidos
Fil: Moreno, Javier Edgardo. Michigan State University; Estados Unidos. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Instituto de Agrobiotecnología del Litoral. Universidad Nacional del Litoral. Instituto de Agrobiotecnología del Litoral; Argentina
Fil: Suzuki, Kaoru. National Institute of Advanced Industrial Science and Technology. Bioproduction Research Institute; Japón
Fil: Howe, Gregg A.. Michigan State University; Estados Unidos
Fil: Ohme Takagi, Masaru. National Institute of Advanced Industrial Science and Technology. Bioproduction Research Institute; Japón. Saitama University. Institute for Environmental Science and Technology ; Japón
Materia
Jam1
Jasmonate
Bhlh
Repressor
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/25194
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/25194
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling A bHLH-type transcription factor, JA-ASSOCIATED MYC2-LIKE1, acts as repressor to negatively regulate jasmonate signaling in ArabidopsisNakata, MasaruMitsuda, NobutakaHerde, MarcoKoo, Abraham J. K.Moreno, Javier EdgardoSuzuki, KaoruHowe, Gregg A.Ohme Takagi, MasaruJam1JasmonateBhlhRepressorhttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1Jasmonates (JAs) are plant hormones that regulate the balance between plant growth and responses to biotic and abiotic stresses. Although recent studies have uncovered the mechanisms for JA-induced responses in Arabidopsis thaliana, the mechanisms by which plants attenuate the JA-induced responses remain elusive. Here, we report that a basic helix-loop-helix-type transcription factor, ABA-INDUCIBLE BHLH-TYPE TRANSCRIPTION FACTOR/JA-ASSOCIATED MYC2-LIKE1 (JAM1), acts as a transcriptional repressor and negatively regulates JA signaling. Gain-of-function transgenic plants expressing the chimeric repressor for JAM1 exhibited substantial reduction of JA responses, including JA-induced inhibition of root growth, accumulation of anthocyanin, and male fertility. These plants were also compromised in resistance to attack by the insect herbivore Spodoptera exigua. Conversely, jam1 loss-of-function mutants showed enhanced JA responsiveness, including increased resistance to insect attack. JAM1 and MYC2 competitively bind to the target sequence of MYC2, which likely provides the mechanism for negative regulation of JA signaling and suppression of MYC2 functions by JAM1. These results indicate that JAM1 negatively regulates JA signaling, thereby playing a pivotal role in fine-tuning of JA-mediated stress responses and plant growth.Fil: Nakata, Masaru. National Institute of Advanced Industrial Science and Technology. Bioproduction Research Institute; JapónFil: Mitsuda, Nobutaka. National Institute of Advanced Industrial Science and Technology. Bioproduction Research Institute; JapónFil: Herde, Marco. Michigan State University; Estados UnidosFil: Koo, Abraham J. K.. Michigan State University; Estados UnidosFil: Moreno, Javier Edgardo. Michigan State University; Estados Unidos. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Instituto de Agrobiotecnología del Litoral. Universidad Nacional del Litoral. Instituto de Agrobiotecnología del Litoral; ArgentinaFil: Suzuki, Kaoru. National Institute of Advanced Industrial Science and Technology. Bioproduction Research Institute; JapónFil: Howe, Gregg A.. Michigan State University; Estados UnidosFil: Ohme Takagi, Masaru. National Institute of Advanced Industrial Science and Technology. Bioproduction Research Institute; Japón. Saitama University. Institute for Environmental Science and Technology ; JapónAmerican Society of Plant Biologist2013-05info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/25194Nakata, Masaru; Mitsuda, Nobutaka; Herde, Marco; Koo, Abraham J. K.; Moreno, Javier Edgardo; et al.; A bHLH-type transcription factor, JA-ASSOCIATED MYC2-LIKE1, acts as repressor to negatively regulate jasmonate signaling in Arabidopsis; American Society of Plant Biologist; Plant Cell; 25; 5; 5-2013; 1641-16561040-4651CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/http://www.plantcell.org/content/25/5/1641info:eu-repo/semantics/altIdentifier/doi/10.1105/tpc.113.111112info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T10:15:55Zoai:ri.conicet.gov.ar:11336/25194instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 10:15:55.597CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv A bHLH-type transcription factor, JA-ASSOCIATED MYC2-LIKE1, acts as repressor to negatively regulate jasmonate signaling in Arabidopsis
title A bHLH-type transcription factor, JA-ASSOCIATED MYC2-LIKE1, acts as repressor to negatively regulate jasmonate signaling in Arabidopsis
spellingShingle A bHLH-type transcription factor, JA-ASSOCIATED MYC2-LIKE1, acts as repressor to negatively regulate jasmonate signaling in Arabidopsis
Nakata, Masaru
Jam1
Jasmonate
Bhlh
Repressor
title_short A bHLH-type transcription factor, JA-ASSOCIATED MYC2-LIKE1, acts as repressor to negatively regulate jasmonate signaling in Arabidopsis
title_full A bHLH-type transcription factor, JA-ASSOCIATED MYC2-LIKE1, acts as repressor to negatively regulate jasmonate signaling in Arabidopsis
title_fullStr A bHLH-type transcription factor, JA-ASSOCIATED MYC2-LIKE1, acts as repressor to negatively regulate jasmonate signaling in Arabidopsis
title_full_unstemmed A bHLH-type transcription factor, JA-ASSOCIATED MYC2-LIKE1, acts as repressor to negatively regulate jasmonate signaling in Arabidopsis
title_sort A bHLH-type transcription factor, JA-ASSOCIATED MYC2-LIKE1, acts as repressor to negatively regulate jasmonate signaling in Arabidopsis
dc.creator.none.fl_str_mv Nakata, Masaru
Mitsuda, Nobutaka
Herde, Marco
Koo, Abraham J. K.
Moreno, Javier Edgardo
Suzuki, Kaoru
Howe, Gregg A.
Ohme Takagi, Masaru
author Nakata, Masaru
author_facet Nakata, Masaru
Mitsuda, Nobutaka
Herde, Marco
Koo, Abraham J. K.
Moreno, Javier Edgardo
Suzuki, Kaoru
Howe, Gregg A.
Ohme Takagi, Masaru
author_role author
author2 Mitsuda, Nobutaka
Herde, Marco
Koo, Abraham J. K.
Moreno, Javier Edgardo
Suzuki, Kaoru
Howe, Gregg A.
Ohme Takagi, Masaru
author2_role author
author
author
author
author
author
author
dc.subject.none.fl_str_mv Jam1
Jasmonate
Bhlh
Repressor
topic Jam1
Jasmonate
Bhlh
Repressor
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv Jasmonates (JAs) are plant hormones that regulate the balance between plant growth and responses to biotic and abiotic stresses. Although recent studies have uncovered the mechanisms for JA-induced responses in Arabidopsis thaliana, the mechanisms by which plants attenuate the JA-induced responses remain elusive. Here, we report that a basic helix-loop-helix-type transcription factor, ABA-INDUCIBLE BHLH-TYPE TRANSCRIPTION FACTOR/JA-ASSOCIATED MYC2-LIKE1 (JAM1), acts as a transcriptional repressor and negatively regulates JA signaling. Gain-of-function transgenic plants expressing the chimeric repressor for JAM1 exhibited substantial reduction of JA responses, including JA-induced inhibition of root growth, accumulation of anthocyanin, and male fertility. These plants were also compromised in resistance to attack by the insect herbivore Spodoptera exigua. Conversely, jam1 loss-of-function mutants showed enhanced JA responsiveness, including increased resistance to insect attack. JAM1 and MYC2 competitively bind to the target sequence of MYC2, which likely provides the mechanism for negative regulation of JA signaling and suppression of MYC2 functions by JAM1. These results indicate that JAM1 negatively regulates JA signaling, thereby playing a pivotal role in fine-tuning of JA-mediated stress responses and plant growth.
Fil: Nakata, Masaru. National Institute of Advanced Industrial Science and Technology. Bioproduction Research Institute; Japón
Fil: Mitsuda, Nobutaka. National Institute of Advanced Industrial Science and Technology. Bioproduction Research Institute; Japón
Fil: Herde, Marco. Michigan State University; Estados Unidos
Fil: Koo, Abraham J. K.. Michigan State University; Estados Unidos
Fil: Moreno, Javier Edgardo. Michigan State University; Estados Unidos. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Instituto de Agrobiotecnología del Litoral. Universidad Nacional del Litoral. Instituto de Agrobiotecnología del Litoral; Argentina
Fil: Suzuki, Kaoru. National Institute of Advanced Industrial Science and Technology. Bioproduction Research Institute; Japón
Fil: Howe, Gregg A.. Michigan State University; Estados Unidos
Fil: Ohme Takagi, Masaru. National Institute of Advanced Industrial Science and Technology. Bioproduction Research Institute; Japón. Saitama University. Institute for Environmental Science and Technology ; Japón
description Jasmonates (JAs) are plant hormones that regulate the balance between plant growth and responses to biotic and abiotic stresses. Although recent studies have uncovered the mechanisms for JA-induced responses in Arabidopsis thaliana, the mechanisms by which plants attenuate the JA-induced responses remain elusive. Here, we report that a basic helix-loop-helix-type transcription factor, ABA-INDUCIBLE BHLH-TYPE TRANSCRIPTION FACTOR/JA-ASSOCIATED MYC2-LIKE1 (JAM1), acts as a transcriptional repressor and negatively regulates JA signaling. Gain-of-function transgenic plants expressing the chimeric repressor for JAM1 exhibited substantial reduction of JA responses, including JA-induced inhibition of root growth, accumulation of anthocyanin, and male fertility. These plants were also compromised in resistance to attack by the insect herbivore Spodoptera exigua. Conversely, jam1 loss-of-function mutants showed enhanced JA responsiveness, including increased resistance to insect attack. JAM1 and MYC2 competitively bind to the target sequence of MYC2, which likely provides the mechanism for negative regulation of JA signaling and suppression of MYC2 functions by JAM1. These results indicate that JAM1 negatively regulates JA signaling, thereby playing a pivotal role in fine-tuning of JA-mediated stress responses and plant growth.
publishDate 2013
dc.date.none.fl_str_mv 2013-05
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/25194
Nakata, Masaru; Mitsuda, Nobutaka; Herde, Marco; Koo, Abraham J. K.; Moreno, Javier Edgardo; et al.; A bHLH-type transcription factor, JA-ASSOCIATED MYC2-LIKE1, acts as repressor to negatively regulate jasmonate signaling in Arabidopsis; American Society of Plant Biologist; Plant Cell; 25; 5; 5-2013; 1641-1656
1040-4651
CONICET Digital
CONICET
url http://hdl.handle.net/11336/25194
identifier_str_mv Nakata, Masaru; Mitsuda, Nobutaka; Herde, Marco; Koo, Abraham J. K.; Moreno, Javier Edgardo; et al.; A bHLH-type transcription factor, JA-ASSOCIATED MYC2-LIKE1, acts as repressor to negatively regulate jasmonate signaling in Arabidopsis; American Society of Plant Biologist; Plant Cell; 25; 5; 5-2013; 1641-1656
1040-4651
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/http://www.plantcell.org/content/25/5/1641
info:eu-repo/semantics/altIdentifier/doi/10.1105/tpc.113.111112
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv American Society of Plant Biologist
publisher.none.fl_str_mv American Society of Plant Biologist
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
_version_ 1844614098584600576
score 13.070432

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