Interference between ER stress-related bZIP-type and jasmonate-inducible bHLH-type transcription factors in the regulation of triterpene saponin biosynthesis in Medicago truncatula

Autores
Ribeiro, Bianca; Erffelinck, Marie Laure; Lacchini, Elia; Ceulemans, Evi; Colinas, Maite; Williams, Clara; Van Hamme, Evelien; De Clercq, Rebecca; Perassolo, Maria; Goossens, Alain
Año de publicación
2022
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Triterpene saponins (TS) are a structurally diverse group of metabolites that are widely distributed in plants. They primarily serve as defense compounds and their production is often triggered by biotic stresses through signaling cascades that are modulated by phytohormones such as the jasmonates (JA). Two JA-modulated basic helix-loop-helix (bHLH) transcription factors (TFs), triterpene saponin biosynthesis activating regulator 1 (TSAR1) and TSAR2, have previously been identified as direct activators of TS biosynthesis in the model legume Medicago truncatula. Here, we report on the involvement of the core endoplasmic reticulum (ER) stress-related basic leucine zipper (bZIP) TFs bZIP17 and bZIP60 in the regulation of TS biosynthesis. Expression and processing of M. truncatula bZIP17 and bZIP60 proteins were altered in roots with perturbed TS biosynthesis or treated with JA. Accordingly, such roots displayed an altered ER network structure. M. truncatula bZIP17 and bZIP60 proteins were shown to localize in the nucleus and appeared to be capable of interfering with the TSAR-mediated transactivation of TS biosynthesis genes. Furthermore, interference between ER stress-related bZIP and JA-modulated bHLH TFs in the regulation of JA-dependent terpene biosynthetic pathways may be widespread in the plant kingdom, as we demonstrate that it also occurs in the regulation of monoterpene indole alkaloid biosynthesis in the medicinal plant Catharanthus roseus.
Fil: Ribeiro, Bianca. Universiteit Gent. Faculty Of Sciences. Departament Of Plant Biotechnology And Bioinformatics.; Bélgica
Fil: Erffelinck, Marie Laure. Universiteit Gent. Faculty Of Sciences. Departament Of Plant Biotechnology And Bioinformatics.; Bélgica
Fil: Lacchini, Elia. Universiteit Gent. Faculty Of Sciences. Departament Of Plant Biotechnology And Bioinformatics.; Bélgica
Fil: Ceulemans, Evi. Universiteit Gent. Faculty Of Sciences. Departament Of Plant Biotechnology And Bioinformatics.; Bélgica
Fil: Colinas, Maite. Universiteit Gent. Faculty Of Sciences. Departament Of Plant Biotechnology And Bioinformatics.; Bélgica
Fil: Williams, Clara. Universiteit Gent. Faculty Of Sciences. Departament Of Plant Biotechnology And Bioinformatics.; Bélgica
Fil: Van Hamme, Evelien. University of Ghent; Bélgica
Fil: De Clercq, Rebecca. Universiteit Gent. Faculty Of Sciences. Departament Of Plant Biotechnology And Bioinformatics.; Bélgica
Fil: Perassolo, Maria. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Departamento de Microbiología, Inmunología y Biotecnología. Cátedra de Microbiología Industrial y Biotecnología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Nanobiotecnología. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Instituto de Nanobiotecnología; Argentina
Fil: Goossens, Alain. Universiteit Gent. Faculty Of Sciences. Departament Of Plant Biotechnology And Bioinformatics.; Bélgica
Materia
BASIC HELIX-LOOP-HELIX
BZIP
CATHARANTHUS
ENDOPLASMIC RETICULUM
JASMONATE
MEDICAGO CATHARANTHUS
SAPONIN
TRITERPENE
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/214742
Acceder
id CONICETDig_62f5eb3755426336f38bfc351ad2f185
oai_identifier_str oai:ri.conicet.gov.ar:11336/214742
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Interference between ER stress-related bZIP-type and jasmonate-inducible bHLH-type transcription factors in the regulation of triterpene saponin biosynthesis in Medicago truncatulaRibeiro, BiancaErffelinck, Marie LaureLacchini, EliaCeulemans, EviColinas, MaiteWilliams, ClaraVan Hamme, EvelienDe Clercq, RebeccaPerassolo, MariaGoossens, AlainBASIC HELIX-LOOP-HELIXBZIPCATHARANTHUSENDOPLASMIC RETICULUMJASMONATEMEDICAGO CATHARANTHUSSAPONINTRITERPENEhttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1Triterpene saponins (TS) are a structurally diverse group of metabolites that are widely distributed in plants. They primarily serve as defense compounds and their production is often triggered by biotic stresses through signaling cascades that are modulated by phytohormones such as the jasmonates (JA). Two JA-modulated basic helix-loop-helix (bHLH) transcription factors (TFs), triterpene saponin biosynthesis activating regulator 1 (TSAR1) and TSAR2, have previously been identified as direct activators of TS biosynthesis in the model legume Medicago truncatula. Here, we report on the involvement of the core endoplasmic reticulum (ER) stress-related basic leucine zipper (bZIP) TFs bZIP17 and bZIP60 in the regulation of TS biosynthesis. Expression and processing of M. truncatula bZIP17 and bZIP60 proteins were altered in roots with perturbed TS biosynthesis or treated with JA. Accordingly, such roots displayed an altered ER network structure. M. truncatula bZIP17 and bZIP60 proteins were shown to localize in the nucleus and appeared to be capable of interfering with the TSAR-mediated transactivation of TS biosynthesis genes. Furthermore, interference between ER stress-related bZIP and JA-modulated bHLH TFs in the regulation of JA-dependent terpene biosynthetic pathways may be widespread in the plant kingdom, as we demonstrate that it also occurs in the regulation of monoterpene indole alkaloid biosynthesis in the medicinal plant Catharanthus roseus.Fil: Ribeiro, Bianca. Universiteit Gent. Faculty Of Sciences. Departament Of Plant Biotechnology And Bioinformatics.; BélgicaFil: Erffelinck, Marie Laure. Universiteit Gent. Faculty Of Sciences. Departament Of Plant Biotechnology And Bioinformatics.; BélgicaFil: Lacchini, Elia. Universiteit Gent. Faculty Of Sciences. Departament Of Plant Biotechnology And Bioinformatics.; BélgicaFil: Ceulemans, Evi. Universiteit Gent. Faculty Of Sciences. Departament Of Plant Biotechnology And Bioinformatics.; BélgicaFil: Colinas, Maite. Universiteit Gent. Faculty Of Sciences. Departament Of Plant Biotechnology And Bioinformatics.; BélgicaFil: Williams, Clara. Universiteit Gent. Faculty Of Sciences. Departament Of Plant Biotechnology And Bioinformatics.; BélgicaFil: Van Hamme, Evelien. University of Ghent; BélgicaFil: De Clercq, Rebecca. Universiteit Gent. Faculty Of Sciences. Departament Of Plant Biotechnology And Bioinformatics.; BélgicaFil: Perassolo, Maria. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Departamento de Microbiología, Inmunología y Biotecnología. Cátedra de Microbiología Industrial y Biotecnología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Nanobiotecnología. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Instituto de Nanobiotecnología; ArgentinaFil: Goossens, Alain. Universiteit Gent. Faculty Of Sciences. Departament Of Plant Biotechnology And Bioinformatics.; BélgicaFrontiers Media2022-09info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/214742Ribeiro, Bianca; Erffelinck, Marie Laure; Lacchini, Elia; Ceulemans, Evi; Colinas, Maite; et al.; Interference between ER stress-related bZIP-type and jasmonate-inducible bHLH-type transcription factors in the regulation of triterpene saponin biosynthesis in Medicago truncatula; Frontiers Media; Frontiers in Plant Science; 13; 9-2022; 1-181664-462XCONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://www.frontiersin.org/articles/10.3389/fpls.2022.903793/fullinfo:eu-repo/semantics/altIdentifier/doi/10.3389/fpls.2022.903793info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T09:33:12Zoai:ri.conicet.gov.ar:11336/214742instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 09:33:13.16CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Interference between ER stress-related bZIP-type and jasmonate-inducible bHLH-type transcription factors in the regulation of triterpene saponin biosynthesis in Medicago truncatula
title Interference between ER stress-related bZIP-type and jasmonate-inducible bHLH-type transcription factors in the regulation of triterpene saponin biosynthesis in Medicago truncatula
spellingShingle Interference between ER stress-related bZIP-type and jasmonate-inducible bHLH-type transcription factors in the regulation of triterpene saponin biosynthesis in Medicago truncatula
Ribeiro, Bianca
BASIC HELIX-LOOP-HELIX
BZIP
CATHARANTHUS
ENDOPLASMIC RETICULUM
JASMONATE
MEDICAGO CATHARANTHUS
SAPONIN
TRITERPENE
title_short Interference between ER stress-related bZIP-type and jasmonate-inducible bHLH-type transcription factors in the regulation of triterpene saponin biosynthesis in Medicago truncatula
title_full Interference between ER stress-related bZIP-type and jasmonate-inducible bHLH-type transcription factors in the regulation of triterpene saponin biosynthesis in Medicago truncatula
title_fullStr Interference between ER stress-related bZIP-type and jasmonate-inducible bHLH-type transcription factors in the regulation of triterpene saponin biosynthesis in Medicago truncatula
title_full_unstemmed Interference between ER stress-related bZIP-type and jasmonate-inducible bHLH-type transcription factors in the regulation of triterpene saponin biosynthesis in Medicago truncatula
title_sort Interference between ER stress-related bZIP-type and jasmonate-inducible bHLH-type transcription factors in the regulation of triterpene saponin biosynthesis in Medicago truncatula
dc.creator.none.fl_str_mv Ribeiro, Bianca
Erffelinck, Marie Laure
Lacchini, Elia
Ceulemans, Evi
Colinas, Maite
Williams, Clara
Van Hamme, Evelien
De Clercq, Rebecca
Perassolo, Maria
Goossens, Alain
author Ribeiro, Bianca
author_facet Ribeiro, Bianca
Erffelinck, Marie Laure
Lacchini, Elia
Ceulemans, Evi
Colinas, Maite
Williams, Clara
Van Hamme, Evelien
De Clercq, Rebecca
Perassolo, Maria
Goossens, Alain
author_role author
author2 Erffelinck, Marie Laure
Lacchini, Elia
Ceulemans, Evi
Colinas, Maite
Williams, Clara
Van Hamme, Evelien
De Clercq, Rebecca
Perassolo, Maria
Goossens, Alain
author2_role author
author
author
author
author
author
author
author
author
dc.subject.none.fl_str_mv BASIC HELIX-LOOP-HELIX
BZIP
CATHARANTHUS
ENDOPLASMIC RETICULUM
JASMONATE
MEDICAGO CATHARANTHUS
SAPONIN
TRITERPENE
topic BASIC HELIX-LOOP-HELIX
BZIP
CATHARANTHUS
ENDOPLASMIC RETICULUM
JASMONATE
MEDICAGO CATHARANTHUS
SAPONIN
TRITERPENE
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv Triterpene saponins (TS) are a structurally diverse group of metabolites that are widely distributed in plants. They primarily serve as defense compounds and their production is often triggered by biotic stresses through signaling cascades that are modulated by phytohormones such as the jasmonates (JA). Two JA-modulated basic helix-loop-helix (bHLH) transcription factors (TFs), triterpene saponin biosynthesis activating regulator 1 (TSAR1) and TSAR2, have previously been identified as direct activators of TS biosynthesis in the model legume Medicago truncatula. Here, we report on the involvement of the core endoplasmic reticulum (ER) stress-related basic leucine zipper (bZIP) TFs bZIP17 and bZIP60 in the regulation of TS biosynthesis. Expression and processing of M. truncatula bZIP17 and bZIP60 proteins were altered in roots with perturbed TS biosynthesis or treated with JA. Accordingly, such roots displayed an altered ER network structure. M. truncatula bZIP17 and bZIP60 proteins were shown to localize in the nucleus and appeared to be capable of interfering with the TSAR-mediated transactivation of TS biosynthesis genes. Furthermore, interference between ER stress-related bZIP and JA-modulated bHLH TFs in the regulation of JA-dependent terpene biosynthetic pathways may be widespread in the plant kingdom, as we demonstrate that it also occurs in the regulation of monoterpene indole alkaloid biosynthesis in the medicinal plant Catharanthus roseus.
Fil: Ribeiro, Bianca. Universiteit Gent. Faculty Of Sciences. Departament Of Plant Biotechnology And Bioinformatics.; Bélgica
Fil: Erffelinck, Marie Laure. Universiteit Gent. Faculty Of Sciences. Departament Of Plant Biotechnology And Bioinformatics.; Bélgica
Fil: Lacchini, Elia. Universiteit Gent. Faculty Of Sciences. Departament Of Plant Biotechnology And Bioinformatics.; Bélgica
Fil: Ceulemans, Evi. Universiteit Gent. Faculty Of Sciences. Departament Of Plant Biotechnology And Bioinformatics.; Bélgica
Fil: Colinas, Maite. Universiteit Gent. Faculty Of Sciences. Departament Of Plant Biotechnology And Bioinformatics.; Bélgica
Fil: Williams, Clara. Universiteit Gent. Faculty Of Sciences. Departament Of Plant Biotechnology And Bioinformatics.; Bélgica
Fil: Van Hamme, Evelien. University of Ghent; Bélgica
Fil: De Clercq, Rebecca. Universiteit Gent. Faculty Of Sciences. Departament Of Plant Biotechnology And Bioinformatics.; Bélgica
Fil: Perassolo, Maria. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Departamento de Microbiología, Inmunología y Biotecnología. Cátedra de Microbiología Industrial y Biotecnología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Nanobiotecnología. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Instituto de Nanobiotecnología; Argentina
Fil: Goossens, Alain. Universiteit Gent. Faculty Of Sciences. Departament Of Plant Biotechnology And Bioinformatics.; Bélgica
description Triterpene saponins (TS) are a structurally diverse group of metabolites that are widely distributed in plants. They primarily serve as defense compounds and their production is often triggered by biotic stresses through signaling cascades that are modulated by phytohormones such as the jasmonates (JA). Two JA-modulated basic helix-loop-helix (bHLH) transcription factors (TFs), triterpene saponin biosynthesis activating regulator 1 (TSAR1) and TSAR2, have previously been identified as direct activators of TS biosynthesis in the model legume Medicago truncatula. Here, we report on the involvement of the core endoplasmic reticulum (ER) stress-related basic leucine zipper (bZIP) TFs bZIP17 and bZIP60 in the regulation of TS biosynthesis. Expression and processing of M. truncatula bZIP17 and bZIP60 proteins were altered in roots with perturbed TS biosynthesis or treated with JA. Accordingly, such roots displayed an altered ER network structure. M. truncatula bZIP17 and bZIP60 proteins were shown to localize in the nucleus and appeared to be capable of interfering with the TSAR-mediated transactivation of TS biosynthesis genes. Furthermore, interference between ER stress-related bZIP and JA-modulated bHLH TFs in the regulation of JA-dependent terpene biosynthetic pathways may be widespread in the plant kingdom, as we demonstrate that it also occurs in the regulation of monoterpene indole alkaloid biosynthesis in the medicinal plant Catharanthus roseus.
publishDate 2022
dc.date.none.fl_str_mv 2022-09
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/214742
Ribeiro, Bianca; Erffelinck, Marie Laure; Lacchini, Elia; Ceulemans, Evi; Colinas, Maite; et al.; Interference between ER stress-related bZIP-type and jasmonate-inducible bHLH-type transcription factors in the regulation of triterpene saponin biosynthesis in Medicago truncatula; Frontiers Media; Frontiers in Plant Science; 13; 9-2022; 1-18
1664-462X
CONICET Digital
CONICET
url http://hdl.handle.net/11336/214742
identifier_str_mv Ribeiro, Bianca; Erffelinck, Marie Laure; Lacchini, Elia; Ceulemans, Evi; Colinas, Maite; et al.; Interference between ER stress-related bZIP-type and jasmonate-inducible bHLH-type transcription factors in the regulation of triterpene saponin biosynthesis in Medicago truncatula; Frontiers Media; Frontiers in Plant Science; 13; 9-2022; 1-18
1664-462X
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://www.frontiersin.org/articles/10.3389/fpls.2022.903793/full
info:eu-repo/semantics/altIdentifier/doi/10.3389/fpls.2022.903793
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Frontiers Media
publisher.none.fl_str_mv Frontiers Media
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.070432

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