Viral diversity of Junín virus field strains

Autores
Goñi, Sandra Elizabeth; Stephan, Betina Inés; Iserte, Javier Alonso; Contigiani de Minio, Marta Silvia; Lozano, Mario Enrique; Antonio Tenorio
Año de publicación
2011
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
The Argentine Hemorrhagic Fever, an endemic disease present in a much of Argentina, is caused by the Junín virus (JUNV). Currently, there are sequences available from several strains of this virus, like those belonging to the vaccine lineage (XJ13, XJ#44 and Candid#1), as well as MC2 (rodent isolate) and IV4454 (human isolate). In this article, we report sequence information on two fragments of genomic segment S of viral isolates from the endemic area. A Nested-RT-PCR was used to amplify discrete genomic regions of 13 isolates of rodent and human origin. The bioinformatics studies revealed a great homogeneity of sequences among the JUNV isolates. The phylogenetic classification showed greater evolutionary distance between the old world arenaviruses (Lassa and LCM virus) than between the new world arenaviruses (JUNV and Machupo virus).
Fil: Goñi, Sandra Elizabeth. Universidad Nacional de Quilmes. Departamento de Ciencia y Tecnología. Laboratorio de Ingeniería Genética y Biología Molecular y Celular; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Stephan, Betina Inés. Universidad Nacional de Quilmes. Departamento de Ciencia y Tecnología. Laboratorio de Ingeniería Genética y Biología Molecular y Celular; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Iserte, Javier Alonso. Universidad Nacional de Quilmes. Departamento de Ciencia y Tecnología. Laboratorio de Ingeniería Genética y Biología Molecular y Celular; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Contigiani de Minio, Marta Silvia. Universidad Nacional de Córdoba. Facultad de Medicina. Instituto de Virología “Dr. J. M. Vanella”; Argentina
Fil: Lozano, Mario Enrique. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional de Quilmes. Departamento de Ciencia y Tecnología. Laboratorio de Ingeniería Genética y Biología Molecular y Celular; Argentina
Fil: Antonio Tenorio. Instituto de Salud Carlos III. Centro Nacional de Microbiología; España
Materia
Arenaviridae
Junin Virus
Evolutive distance
Argentine Hemorrhagic Fever
RT-Nested-PCR
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/236776
Acceder
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network_name_str CONICET Digital (CONICET)
spelling Viral diversity of Junín virus field strainsGoñi, Sandra ElizabethStephan, Betina InésIserte, Javier AlonsoContigiani de Minio, Marta SilviaLozano, Mario EnriqueAntonio TenorioArenaviridaeJunin VirusEvolutive distanceArgentine Hemorrhagic FeverRT-Nested-PCRhttps://purl.org/becyt/ford/3.4https://purl.org/becyt/ford/3The Argentine Hemorrhagic Fever, an endemic disease present in a much of Argentina, is caused by the Junín virus (JUNV). Currently, there are sequences available from several strains of this virus, like those belonging to the vaccine lineage (XJ13, XJ#44 and Candid#1), as well as MC2 (rodent isolate) and IV4454 (human isolate). In this article, we report sequence information on two fragments of genomic segment S of viral isolates from the endemic area. A Nested-RT-PCR was used to amplify discrete genomic regions of 13 isolates of rodent and human origin. The bioinformatics studies revealed a great homogeneity of sequences among the JUNV isolates. The phylogenetic classification showed greater evolutionary distance between the old world arenaviruses (Lassa and LCM virus) than between the new world arenaviruses (JUNV and Machupo virus).Fil: Goñi, Sandra Elizabeth. Universidad Nacional de Quilmes. Departamento de Ciencia y Tecnología. Laboratorio de Ingeniería Genética y Biología Molecular y Celular; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Stephan, Betina Inés. Universidad Nacional de Quilmes. Departamento de Ciencia y Tecnología. Laboratorio de Ingeniería Genética y Biología Molecular y Celular; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Iserte, Javier Alonso. Universidad Nacional de Quilmes. Departamento de Ciencia y Tecnología. Laboratorio de Ingeniería Genética y Biología Molecular y Celular; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Contigiani de Minio, Marta Silvia. Universidad Nacional de Córdoba. Facultad de Medicina. Instituto de Virología “Dr. J. M. Vanella”; ArgentinaFil: Lozano, Mario Enrique. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional de Quilmes. Departamento de Ciencia y Tecnología. Laboratorio de Ingeniería Genética y Biología Molecular y Celular; ArgentinaFil: Antonio Tenorio. Instituto de Salud Carlos III. Centro Nacional de Microbiología; EspañaElsevier Science2011-09info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/236776Goñi, Sandra Elizabeth; Stephan, Betina Inés; Iserte, Javier Alonso; Contigiani de Minio, Marta Silvia; Lozano, Mario Enrique; et al.; Viral diversity of Junín virus field strains; Elsevier Science; Virus Research; 160; 1-2; 9-2011; 150-1580168-1702CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/pii/S0168170211002279info:eu-repo/semantics/altIdentifier/doi/10.1016/j.virusres.2011.06.004info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2026-02-26T10:34:48Zoai:ri.conicet.gov.ar:11336/236776instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982026-02-26 10:34:49.025CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Viral diversity of Junín virus field strains
title Viral diversity of Junín virus field strains
spellingShingle Viral diversity of Junín virus field strains
Goñi, Sandra Elizabeth
Arenaviridae
Junin Virus
Evolutive distance
Argentine Hemorrhagic Fever
RT-Nested-PCR
title_short Viral diversity of Junín virus field strains
title_full Viral diversity of Junín virus field strains
title_fullStr Viral diversity of Junín virus field strains
title_full_unstemmed Viral diversity of Junín virus field strains
title_sort Viral diversity of Junín virus field strains
dc.creator.none.fl_str_mv Goñi, Sandra Elizabeth
Stephan, Betina Inés
Iserte, Javier Alonso
Contigiani de Minio, Marta Silvia
Lozano, Mario Enrique
Antonio Tenorio
author Goñi, Sandra Elizabeth
author_facet Goñi, Sandra Elizabeth
Stephan, Betina Inés
Iserte, Javier Alonso
Contigiani de Minio, Marta Silvia
Lozano, Mario Enrique
Antonio Tenorio
author_role author
author2 Stephan, Betina Inés
Iserte, Javier Alonso
Contigiani de Minio, Marta Silvia
Lozano, Mario Enrique
Antonio Tenorio
author2_role author
author
author
author
author
dc.subject.none.fl_str_mv Arenaviridae
Junin Virus
Evolutive distance
Argentine Hemorrhagic Fever
RT-Nested-PCR
topic Arenaviridae
Junin Virus
Evolutive distance
Argentine Hemorrhagic Fever
RT-Nested-PCR
purl_subject.fl_str_mv https://purl.org/becyt/ford/3.4
https://purl.org/becyt/ford/3
dc.description.none.fl_txt_mv The Argentine Hemorrhagic Fever, an endemic disease present in a much of Argentina, is caused by the Junín virus (JUNV). Currently, there are sequences available from several strains of this virus, like those belonging to the vaccine lineage (XJ13, XJ#44 and Candid#1), as well as MC2 (rodent isolate) and IV4454 (human isolate). In this article, we report sequence information on two fragments of genomic segment S of viral isolates from the endemic area. A Nested-RT-PCR was used to amplify discrete genomic regions of 13 isolates of rodent and human origin. The bioinformatics studies revealed a great homogeneity of sequences among the JUNV isolates. The phylogenetic classification showed greater evolutionary distance between the old world arenaviruses (Lassa and LCM virus) than between the new world arenaviruses (JUNV and Machupo virus).
Fil: Goñi, Sandra Elizabeth. Universidad Nacional de Quilmes. Departamento de Ciencia y Tecnología. Laboratorio de Ingeniería Genética y Biología Molecular y Celular; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Stephan, Betina Inés. Universidad Nacional de Quilmes. Departamento de Ciencia y Tecnología. Laboratorio de Ingeniería Genética y Biología Molecular y Celular; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Iserte, Javier Alonso. Universidad Nacional de Quilmes. Departamento de Ciencia y Tecnología. Laboratorio de Ingeniería Genética y Biología Molecular y Celular; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Contigiani de Minio, Marta Silvia. Universidad Nacional de Córdoba. Facultad de Medicina. Instituto de Virología “Dr. J. M. Vanella”; Argentina
Fil: Lozano, Mario Enrique. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional de Quilmes. Departamento de Ciencia y Tecnología. Laboratorio de Ingeniería Genética y Biología Molecular y Celular; Argentina
Fil: Antonio Tenorio. Instituto de Salud Carlos III. Centro Nacional de Microbiología; España
description The Argentine Hemorrhagic Fever, an endemic disease present in a much of Argentina, is caused by the Junín virus (JUNV). Currently, there are sequences available from several strains of this virus, like those belonging to the vaccine lineage (XJ13, XJ#44 and Candid#1), as well as MC2 (rodent isolate) and IV4454 (human isolate). In this article, we report sequence information on two fragments of genomic segment S of viral isolates from the endemic area. A Nested-RT-PCR was used to amplify discrete genomic regions of 13 isolates of rodent and human origin. The bioinformatics studies revealed a great homogeneity of sequences among the JUNV isolates. The phylogenetic classification showed greater evolutionary distance between the old world arenaviruses (Lassa and LCM virus) than between the new world arenaviruses (JUNV and Machupo virus).
publishDate 2011
dc.date.none.fl_str_mv 2011-09
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/236776
Goñi, Sandra Elizabeth; Stephan, Betina Inés; Iserte, Javier Alonso; Contigiani de Minio, Marta Silvia; Lozano, Mario Enrique; et al.; Viral diversity of Junín virus field strains; Elsevier Science; Virus Research; 160; 1-2; 9-2011; 150-158
0168-1702
CONICET Digital
CONICET
url http://hdl.handle.net/11336/236776
identifier_str_mv Goñi, Sandra Elizabeth; Stephan, Betina Inés; Iserte, Javier Alonso; Contigiani de Minio, Marta Silvia; Lozano, Mario Enrique; et al.; Viral diversity of Junín virus field strains; Elsevier Science; Virus Research; 160; 1-2; 9-2011; 150-158
0168-1702
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/pii/S0168170211002279
info:eu-repo/semantics/altIdentifier/doi/10.1016/j.virusres.2011.06.004
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Elsevier Science
publisher.none.fl_str_mv Elsevier Science
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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