Revised phylogenetic relationships within the Drosophila buzzatii species cluster (Diptera: Drosophilidae: Drosophila repleta group) using genomic data

Autores
Hurtado, Juan Pablo; Cunha Almeida, Francisca; Revale, Santiago; Hasson, Esteban Ruben
Año de publicación
2019
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
The Drosophila buzzatii cluster is a South American clade that encompasses seven closely related cactophilic species and constitutes a valuable model system for evolutionary research. Though the monophyly of the cluster is strongly supported by molecular, cytological and morphological evidence, phylogenetic relationships within it are still controversial. The phylogeny of the D. buzzatii cluster has been addressed using limited sets of molecular markers, namely a few nuclear and mitochondrial genes, and the sharing of fxed chromosomal inversions. However, analyses based on these data revealed inconsistencies across markers and resulted in poorly resolved basal branches. Here, we revise the phylogeny of the D. buzzatii cluster based on a large transcriptomic dataset of 813 kb obtained from four members of this cluster: D. antonietae, D. borborema, D. buzzatii and D. koepferae, using the close relative D. mojavensis (also a member of the repleta group) as outgroup. Our phylogenomic analyses confrm that D. buzzatii is sister to the other six members of the cluster and, though incomplete lineage sorting likely obstructs phylogenetic resolution among these six species, allowed us to recover a novel topology. Divergence time estimates date the radiation of the cluster to the recent upper Pleistocene with most speciation events compressed to the last 500,000 years.
Fil: Hurtado, Juan Pablo. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Ecología, Genética y Evolución de Buenos Aires. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Instituto de Ecología, Genética y Evolución de Buenos Aires; Argentina. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Ecología, Genética y Evolución; Argentina
Fil: Cunha Almeida, Francisca. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Ecología, Genética y Evolución de Buenos Aires. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Instituto de Ecología, Genética y Evolución de Buenos Aires; Argentina. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Ecología, Genética y Evolución; Argentina
Fil: Revale, Santiago. University of Oxford; Reino Unido
Fil: Hasson, Esteban Ruben. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Ecología, Genética y Evolución de Buenos Aires. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Instituto de Ecología, Genética y Evolución de Buenos Aires; Argentina. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Ecología, Genética y Evolución; Argentina
Materia
Cactophilic Drosophila
Transcriptome assembling
Phylogenimics
Divergence time
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/182708
Acceder
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network_name_str CONICET Digital (CONICET)
spelling Revised phylogenetic relationships within the Drosophila buzzatii species cluster (Diptera: Drosophilidae: Drosophila repleta group) using genomic dataHurtado, Juan PabloCunha Almeida, FranciscaRevale, SantiagoHasson, Esteban RubenCactophilic DrosophilaTranscriptome assemblingPhylogenimicsDivergence timehttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1The Drosophila buzzatii cluster is a South American clade that encompasses seven closely related cactophilic species and constitutes a valuable model system for evolutionary research. Though the monophyly of the cluster is strongly supported by molecular, cytological and morphological evidence, phylogenetic relationships within it are still controversial. The phylogeny of the D. buzzatii cluster has been addressed using limited sets of molecular markers, namely a few nuclear and mitochondrial genes, and the sharing of fxed chromosomal inversions. However, analyses based on these data revealed inconsistencies across markers and resulted in poorly resolved basal branches. Here, we revise the phylogeny of the D. buzzatii cluster based on a large transcriptomic dataset of 813 kb obtained from four members of this cluster: D. antonietae, D. borborema, D. buzzatii and D. koepferae, using the close relative D. mojavensis (also a member of the repleta group) as outgroup. Our phylogenomic analyses confrm that D. buzzatii is sister to the other six members of the cluster and, though incomplete lineage sorting likely obstructs phylogenetic resolution among these six species, allowed us to recover a novel topology. Divergence time estimates date the radiation of the cluster to the recent upper Pleistocene with most speciation events compressed to the last 500,000 years.Fil: Hurtado, Juan Pablo. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Ecología, Genética y Evolución de Buenos Aires. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Instituto de Ecología, Genética y Evolución de Buenos Aires; Argentina. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Ecología, Genética y Evolución; ArgentinaFil: Cunha Almeida, Francisca. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Ecología, Genética y Evolución de Buenos Aires. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Instituto de Ecología, Genética y Evolución de Buenos Aires; Argentina. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Ecología, Genética y Evolución; ArgentinaFil: Revale, Santiago. University of Oxford; Reino UnidoFil: Hasson, Esteban Ruben. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Ecología, Genética y Evolución de Buenos Aires. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Instituto de Ecología, Genética y Evolución de Buenos Aires; Argentina. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Ecología, Genética y Evolución; ArgentinaSenckenberg Gesellschaft für Naturforschung2019-08info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/vnd.openxmlformats-officedocument.wordprocessingml.documentapplication/pdfhttp://hdl.handle.net/11336/182708Hurtado, Juan Pablo; Cunha Almeida, Francisca; Revale, Santiago; Hasson, Esteban Ruben; Revised phylogenetic relationships within the Drosophila buzzatii species cluster (Diptera: Drosophilidae: Drosophila repleta group) using genomic data; Senckenberg Gesellschaft für Naturforschung; Arthropod systematics & phylogeny; 77; 2; 8-2019; 239-2501863-72211864-8312CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://www.zobodat.at/pdf/Arthropod-Systematics-Phylogeny_77_0239-0250.pdfinfo:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-10-15T14:46:41Zoai:ri.conicet.gov.ar:11336/182708instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-10-15 14:46:41.819CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Revised phylogenetic relationships within the Drosophila buzzatii species cluster (Diptera: Drosophilidae: Drosophila repleta group) using genomic data
title Revised phylogenetic relationships within the Drosophila buzzatii species cluster (Diptera: Drosophilidae: Drosophila repleta group) using genomic data
spellingShingle Revised phylogenetic relationships within the Drosophila buzzatii species cluster (Diptera: Drosophilidae: Drosophila repleta group) using genomic data
Hurtado, Juan Pablo
Cactophilic Drosophila
Transcriptome assembling
Phylogenimics
Divergence time
title_short Revised phylogenetic relationships within the Drosophila buzzatii species cluster (Diptera: Drosophilidae: Drosophila repleta group) using genomic data
title_full Revised phylogenetic relationships within the Drosophila buzzatii species cluster (Diptera: Drosophilidae: Drosophila repleta group) using genomic data
title_fullStr Revised phylogenetic relationships within the Drosophila buzzatii species cluster (Diptera: Drosophilidae: Drosophila repleta group) using genomic data
title_full_unstemmed Revised phylogenetic relationships within the Drosophila buzzatii species cluster (Diptera: Drosophilidae: Drosophila repleta group) using genomic data
title_sort Revised phylogenetic relationships within the Drosophila buzzatii species cluster (Diptera: Drosophilidae: Drosophila repleta group) using genomic data
dc.creator.none.fl_str_mv Hurtado, Juan Pablo
Cunha Almeida, Francisca
Revale, Santiago
Hasson, Esteban Ruben
author Hurtado, Juan Pablo
author_facet Hurtado, Juan Pablo
Cunha Almeida, Francisca
Revale, Santiago
Hasson, Esteban Ruben
author_role author
author2 Cunha Almeida, Francisca
Revale, Santiago
Hasson, Esteban Ruben
author2_role author
author
author
dc.subject.none.fl_str_mv Cactophilic Drosophila
Transcriptome assembling
Phylogenimics
Divergence time
topic Cactophilic Drosophila
Transcriptome assembling
Phylogenimics
Divergence time
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv The Drosophila buzzatii cluster is a South American clade that encompasses seven closely related cactophilic species and constitutes a valuable model system for evolutionary research. Though the monophyly of the cluster is strongly supported by molecular, cytological and morphological evidence, phylogenetic relationships within it are still controversial. The phylogeny of the D. buzzatii cluster has been addressed using limited sets of molecular markers, namely a few nuclear and mitochondrial genes, and the sharing of fxed chromosomal inversions. However, analyses based on these data revealed inconsistencies across markers and resulted in poorly resolved basal branches. Here, we revise the phylogeny of the D. buzzatii cluster based on a large transcriptomic dataset of 813 kb obtained from four members of this cluster: D. antonietae, D. borborema, D. buzzatii and D. koepferae, using the close relative D. mojavensis (also a member of the repleta group) as outgroup. Our phylogenomic analyses confrm that D. buzzatii is sister to the other six members of the cluster and, though incomplete lineage sorting likely obstructs phylogenetic resolution among these six species, allowed us to recover a novel topology. Divergence time estimates date the radiation of the cluster to the recent upper Pleistocene with most speciation events compressed to the last 500,000 years.
Fil: Hurtado, Juan Pablo. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Ecología, Genética y Evolución de Buenos Aires. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Instituto de Ecología, Genética y Evolución de Buenos Aires; Argentina. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Ecología, Genética y Evolución; Argentina
Fil: Cunha Almeida, Francisca. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Ecología, Genética y Evolución de Buenos Aires. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Instituto de Ecología, Genética y Evolución de Buenos Aires; Argentina. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Ecología, Genética y Evolución; Argentina
Fil: Revale, Santiago. University of Oxford; Reino Unido
Fil: Hasson, Esteban Ruben. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Ecología, Genética y Evolución de Buenos Aires. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Instituto de Ecología, Genética y Evolución de Buenos Aires; Argentina. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Ecología, Genética y Evolución; Argentina
description The Drosophila buzzatii cluster is a South American clade that encompasses seven closely related cactophilic species and constitutes a valuable model system for evolutionary research. Though the monophyly of the cluster is strongly supported by molecular, cytological and morphological evidence, phylogenetic relationships within it are still controversial. The phylogeny of the D. buzzatii cluster has been addressed using limited sets of molecular markers, namely a few nuclear and mitochondrial genes, and the sharing of fxed chromosomal inversions. However, analyses based on these data revealed inconsistencies across markers and resulted in poorly resolved basal branches. Here, we revise the phylogeny of the D. buzzatii cluster based on a large transcriptomic dataset of 813 kb obtained from four members of this cluster: D. antonietae, D. borborema, D. buzzatii and D. koepferae, using the close relative D. mojavensis (also a member of the repleta group) as outgroup. Our phylogenomic analyses confrm that D. buzzatii is sister to the other six members of the cluster and, though incomplete lineage sorting likely obstructs phylogenetic resolution among these six species, allowed us to recover a novel topology. Divergence time estimates date the radiation of the cluster to the recent upper Pleistocene with most speciation events compressed to the last 500,000 years.
publishDate 2019
dc.date.none.fl_str_mv 2019-08
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/182708
Hurtado, Juan Pablo; Cunha Almeida, Francisca; Revale, Santiago; Hasson, Esteban Ruben; Revised phylogenetic relationships within the Drosophila buzzatii species cluster (Diptera: Drosophilidae: Drosophila repleta group) using genomic data; Senckenberg Gesellschaft für Naturforschung; Arthropod systematics & phylogeny; 77; 2; 8-2019; 239-250
1863-7221
1864-8312
CONICET Digital
CONICET
url http://hdl.handle.net/11336/182708
identifier_str_mv Hurtado, Juan Pablo; Cunha Almeida, Francisca; Revale, Santiago; Hasson, Esteban Ruben; Revised phylogenetic relationships within the Drosophila buzzatii species cluster (Diptera: Drosophilidae: Drosophila repleta group) using genomic data; Senckenberg Gesellschaft für Naturforschung; Arthropod systematics & phylogeny; 77; 2; 8-2019; 239-250
1863-7221
1864-8312
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://www.zobodat.at/pdf/Arthropod-Systematics-Phylogeny_77_0239-0250.pdf
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/vnd.openxmlformats-officedocument.wordprocessingml.document
application/pdf
dc.publisher.none.fl_str_mv Senckenberg Gesellschaft für Naturforschung
publisher.none.fl_str_mv Senckenberg Gesellschaft für Naturforschung
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.22299

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