Revised phylogenetic relationships within the Drosophila buzzatii species cluster (Diptera: Drosophilidae: Drosophila repleta group) using genomic data
- Autores
- Hurtado, Juan Pablo; Cunha Almeida, Francisca; Revale, Santiago; Hasson, Esteban Ruben
- Año de publicación
- 2019
- Idioma
- inglés
- Tipo de recurso
- artículo
- Estado
- versión publicada
- Descripción
- The Drosophila buzzatii cluster is a South American clade that encompasses seven closely related cactophilic species and constitutes a valuable model system for evolutionary research. Though the monophyly of the cluster is strongly supported by molecular, cytological and morphological evidence, phylogenetic relationships within it are still controversial. The phylogeny of the D. buzzatii cluster has been addressed using limited sets of molecular markers, namely a few nuclear and mitochondrial genes, and the sharing of fxed chromosomal inversions. However, analyses based on these data revealed inconsistencies across markers and resulted in poorly resolved basal branches. Here, we revise the phylogeny of the D. buzzatii cluster based on a large transcriptomic dataset of 813 kb obtained from four members of this cluster: D. antonietae, D. borborema, D. buzzatii and D. koepferae, using the close relative D. mojavensis (also a member of the repleta group) as outgroup. Our phylogenomic analyses confrm that D. buzzatii is sister to the other six members of the cluster and, though incomplete lineage sorting likely obstructs phylogenetic resolution among these six species, allowed us to recover a novel topology. Divergence time estimates date the radiation of the cluster to the recent upper Pleistocene with most speciation events compressed to the last 500,000 years.
Fil: Hurtado, Juan Pablo. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Ecología, Genética y Evolución de Buenos Aires. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Instituto de Ecología, Genética y Evolución de Buenos Aires; Argentina. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Ecología, Genética y Evolución; Argentina
Fil: Cunha Almeida, Francisca. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Ecología, Genética y Evolución de Buenos Aires. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Instituto de Ecología, Genética y Evolución de Buenos Aires; Argentina. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Ecología, Genética y Evolución; Argentina
Fil: Revale, Santiago. University of Oxford; Reino Unido
Fil: Hasson, Esteban Ruben. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Ecología, Genética y Evolución de Buenos Aires. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Instituto de Ecología, Genética y Evolución de Buenos Aires; Argentina. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Ecología, Genética y Evolución; Argentina - Materia
-
Cactophilic Drosophila
Transcriptome assembling
Phylogenimics
Divergence time - Nivel de accesibilidad
- acceso abierto
- Condiciones de uso
- https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
- Repositorio
- Institución
- Consejo Nacional de Investigaciones Científicas y Técnicas
- OAI Identificador
- oai:ri.conicet.gov.ar:11336/182708
Ver los metadatos del registro completo
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Revised phylogenetic relationships within the Drosophila buzzatii species cluster (Diptera: Drosophilidae: Drosophila repleta group) using genomic dataHurtado, Juan PabloCunha Almeida, FranciscaRevale, SantiagoHasson, Esteban RubenCactophilic DrosophilaTranscriptome assemblingPhylogenimicsDivergence timehttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1The Drosophila buzzatii cluster is a South American clade that encompasses seven closely related cactophilic species and constitutes a valuable model system for evolutionary research. Though the monophyly of the cluster is strongly supported by molecular, cytological and morphological evidence, phylogenetic relationships within it are still controversial. The phylogeny of the D. buzzatii cluster has been addressed using limited sets of molecular markers, namely a few nuclear and mitochondrial genes, and the sharing of fxed chromosomal inversions. However, analyses based on these data revealed inconsistencies across markers and resulted in poorly resolved basal branches. Here, we revise the phylogeny of the D. buzzatii cluster based on a large transcriptomic dataset of 813 kb obtained from four members of this cluster: D. antonietae, D. borborema, D. buzzatii and D. koepferae, using the close relative D. mojavensis (also a member of the repleta group) as outgroup. Our phylogenomic analyses confrm that D. buzzatii is sister to the other six members of the cluster and, though incomplete lineage sorting likely obstructs phylogenetic resolution among these six species, allowed us to recover a novel topology. Divergence time estimates date the radiation of the cluster to the recent upper Pleistocene with most speciation events compressed to the last 500,000 years.Fil: Hurtado, Juan Pablo. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Ecología, Genética y Evolución de Buenos Aires. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Instituto de Ecología, Genética y Evolución de Buenos Aires; Argentina. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Ecología, Genética y Evolución; ArgentinaFil: Cunha Almeida, Francisca. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Ecología, Genética y Evolución de Buenos Aires. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Instituto de Ecología, Genética y Evolución de Buenos Aires; Argentina. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Ecología, Genética y Evolución; ArgentinaFil: Revale, Santiago. University of Oxford; Reino UnidoFil: Hasson, Esteban Ruben. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Ecología, Genética y Evolución de Buenos Aires. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Instituto de Ecología, Genética y Evolución de Buenos Aires; Argentina. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Ecología, Genética y Evolución; ArgentinaSenckenberg Gesellschaft für Naturforschung2019-08info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/vnd.openxmlformats-officedocument.wordprocessingml.documentapplication/pdfhttp://hdl.handle.net/11336/182708Hurtado, Juan Pablo; Cunha Almeida, Francisca; Revale, Santiago; Hasson, Esteban Ruben; Revised phylogenetic relationships within the Drosophila buzzatii species cluster (Diptera: Drosophilidae: Drosophila repleta group) using genomic data; Senckenberg Gesellschaft für Naturforschung; Arthropod systematics & phylogeny; 77; 2; 8-2019; 239-2501863-72211864-8312CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://www.zobodat.at/pdf/Arthropod-Systematics-Phylogeny_77_0239-0250.pdfinfo:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T09:51:56Zoai:ri.conicet.gov.ar:11336/182708instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 09:51:56.889CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse |
dc.title.none.fl_str_mv |
Revised phylogenetic relationships within the Drosophila buzzatii species cluster (Diptera: Drosophilidae: Drosophila repleta group) using genomic data |
title |
Revised phylogenetic relationships within the Drosophila buzzatii species cluster (Diptera: Drosophilidae: Drosophila repleta group) using genomic data |
spellingShingle |
Revised phylogenetic relationships within the Drosophila buzzatii species cluster (Diptera: Drosophilidae: Drosophila repleta group) using genomic data Hurtado, Juan Pablo Cactophilic Drosophila Transcriptome assembling Phylogenimics Divergence time |
title_short |
Revised phylogenetic relationships within the Drosophila buzzatii species cluster (Diptera: Drosophilidae: Drosophila repleta group) using genomic data |
title_full |
Revised phylogenetic relationships within the Drosophila buzzatii species cluster (Diptera: Drosophilidae: Drosophila repleta group) using genomic data |
title_fullStr |
Revised phylogenetic relationships within the Drosophila buzzatii species cluster (Diptera: Drosophilidae: Drosophila repleta group) using genomic data |
title_full_unstemmed |
Revised phylogenetic relationships within the Drosophila buzzatii species cluster (Diptera: Drosophilidae: Drosophila repleta group) using genomic data |
title_sort |
Revised phylogenetic relationships within the Drosophila buzzatii species cluster (Diptera: Drosophilidae: Drosophila repleta group) using genomic data |
dc.creator.none.fl_str_mv |
Hurtado, Juan Pablo Cunha Almeida, Francisca Revale, Santiago Hasson, Esteban Ruben |
author |
Hurtado, Juan Pablo |
author_facet |
Hurtado, Juan Pablo Cunha Almeida, Francisca Revale, Santiago Hasson, Esteban Ruben |
author_role |
author |
author2 |
Cunha Almeida, Francisca Revale, Santiago Hasson, Esteban Ruben |
author2_role |
author author author |
dc.subject.none.fl_str_mv |
Cactophilic Drosophila Transcriptome assembling Phylogenimics Divergence time |
topic |
Cactophilic Drosophila Transcriptome assembling Phylogenimics Divergence time |
purl_subject.fl_str_mv |
https://purl.org/becyt/ford/1.6 https://purl.org/becyt/ford/1 |
dc.description.none.fl_txt_mv |
The Drosophila buzzatii cluster is a South American clade that encompasses seven closely related cactophilic species and constitutes a valuable model system for evolutionary research. Though the monophyly of the cluster is strongly supported by molecular, cytological and morphological evidence, phylogenetic relationships within it are still controversial. The phylogeny of the D. buzzatii cluster has been addressed using limited sets of molecular markers, namely a few nuclear and mitochondrial genes, and the sharing of fxed chromosomal inversions. However, analyses based on these data revealed inconsistencies across markers and resulted in poorly resolved basal branches. Here, we revise the phylogeny of the D. buzzatii cluster based on a large transcriptomic dataset of 813 kb obtained from four members of this cluster: D. antonietae, D. borborema, D. buzzatii and D. koepferae, using the close relative D. mojavensis (also a member of the repleta group) as outgroup. Our phylogenomic analyses confrm that D. buzzatii is sister to the other six members of the cluster and, though incomplete lineage sorting likely obstructs phylogenetic resolution among these six species, allowed us to recover a novel topology. Divergence time estimates date the radiation of the cluster to the recent upper Pleistocene with most speciation events compressed to the last 500,000 years. Fil: Hurtado, Juan Pablo. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Ecología, Genética y Evolución de Buenos Aires. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Instituto de Ecología, Genética y Evolución de Buenos Aires; Argentina. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Ecología, Genética y Evolución; Argentina Fil: Cunha Almeida, Francisca. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Ecología, Genética y Evolución de Buenos Aires. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Instituto de Ecología, Genética y Evolución de Buenos Aires; Argentina. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Ecología, Genética y Evolución; Argentina Fil: Revale, Santiago. University of Oxford; Reino Unido Fil: Hasson, Esteban Ruben. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Ecología, Genética y Evolución de Buenos Aires. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Instituto de Ecología, Genética y Evolución de Buenos Aires; Argentina. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Ecología, Genética y Evolución; Argentina |
description |
The Drosophila buzzatii cluster is a South American clade that encompasses seven closely related cactophilic species and constitutes a valuable model system for evolutionary research. Though the monophyly of the cluster is strongly supported by molecular, cytological and morphological evidence, phylogenetic relationships within it are still controversial. The phylogeny of the D. buzzatii cluster has been addressed using limited sets of molecular markers, namely a few nuclear and mitochondrial genes, and the sharing of fxed chromosomal inversions. However, analyses based on these data revealed inconsistencies across markers and resulted in poorly resolved basal branches. Here, we revise the phylogeny of the D. buzzatii cluster based on a large transcriptomic dataset of 813 kb obtained from four members of this cluster: D. antonietae, D. borborema, D. buzzatii and D. koepferae, using the close relative D. mojavensis (also a member of the repleta group) as outgroup. Our phylogenomic analyses confrm that D. buzzatii is sister to the other six members of the cluster and, though incomplete lineage sorting likely obstructs phylogenetic resolution among these six species, allowed us to recover a novel topology. Divergence time estimates date the radiation of the cluster to the recent upper Pleistocene with most speciation events compressed to the last 500,000 years. |
publishDate |
2019 |
dc.date.none.fl_str_mv |
2019-08 |
dc.type.none.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion http://purl.org/coar/resource_type/c_6501 info:ar-repo/semantics/articulo |
format |
article |
status_str |
publishedVersion |
dc.identifier.none.fl_str_mv |
http://hdl.handle.net/11336/182708 Hurtado, Juan Pablo; Cunha Almeida, Francisca; Revale, Santiago; Hasson, Esteban Ruben; Revised phylogenetic relationships within the Drosophila buzzatii species cluster (Diptera: Drosophilidae: Drosophila repleta group) using genomic data; Senckenberg Gesellschaft für Naturforschung; Arthropod systematics & phylogeny; 77; 2; 8-2019; 239-250 1863-7221 1864-8312 CONICET Digital CONICET |
url |
http://hdl.handle.net/11336/182708 |
identifier_str_mv |
Hurtado, Juan Pablo; Cunha Almeida, Francisca; Revale, Santiago; Hasson, Esteban Ruben; Revised phylogenetic relationships within the Drosophila buzzatii species cluster (Diptera: Drosophilidae: Drosophila repleta group) using genomic data; Senckenberg Gesellschaft für Naturforschung; Arthropod systematics & phylogeny; 77; 2; 8-2019; 239-250 1863-7221 1864-8312 CONICET Digital CONICET |
dc.language.none.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
info:eu-repo/semantics/altIdentifier/url/https://www.zobodat.at/pdf/Arthropod-Systematics-Phylogeny_77_0239-0250.pdf |
dc.rights.none.fl_str_mv |
info:eu-repo/semantics/openAccess https://creativecommons.org/licenses/by-nc-sa/2.5/ar/ |
eu_rights_str_mv |
openAccess |
rights_invalid_str_mv |
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/ |
dc.format.none.fl_str_mv |
application/pdf application/vnd.openxmlformats-officedocument.wordprocessingml.document application/pdf |
dc.publisher.none.fl_str_mv |
Senckenberg Gesellschaft für Naturforschung |
publisher.none.fl_str_mv |
Senckenberg Gesellschaft für Naturforschung |
dc.source.none.fl_str_mv |
reponame:CONICET Digital (CONICET) instname:Consejo Nacional de Investigaciones Científicas y Técnicas |
reponame_str |
CONICET Digital (CONICET) |
collection |
CONICET Digital (CONICET) |
instname_str |
Consejo Nacional de Investigaciones Científicas y Técnicas |
repository.name.fl_str_mv |
CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas |
repository.mail.fl_str_mv |
dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar |
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1844613594856030208 |
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13.070432 |