Involvement of the oestrogenic receptors in superior mesenteric ganglion on the ovarian steroidogenesis in rat

Autores
Vega Orozco, Adriana Soledad; Daneri Becerra, Cristina del Rosario; Anesetti, Gabriel; Cabrera Kreiker, Ricardo Jorge; Sosa, Zulema Yolanda; Rastrilla, Ana Maria
Año de publicación
2012
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Oestradiol (E2) is a key hormone in the regulation of reproductive processes. The aims of this work were a) to examine the distributions of oestrogen receptor a (ERa) and ERb in the neurons of the superior mesenteric ganglion (SMG) in the oestrus stage by immunohistochemistry, b) to demonstrate whether E2 in the SMG modifies progesterone (P4), androstenedione (A2) and nitrite release in the ovarian compartment on oestrus day and c) to demonstrate whether E2 in the ganglion modifies the activity and gene expression in the ovary of the steroidogenic enzymes 3b-hydroxysteroid dehydrogenase (3b-HSD) and 20a-hydroxysteroid dehydrogenase (20a-HSD). The ex vivo SMG–ovarian nervous plexus–ovary system was used. E2, tamoxifen (Txf) and E2 plus Txf were added in the ganglion to measure ovarian P4 release, while E2 alone was added to measure ovarian A2 and nitrites release. Immunohistochemistry revealed cytoplasmic ERa immunoreactivity only in the neural somas in the SMG. E2 increased ovarian P4 and A2 release at 15, 30 and 60 min but decreased nitrites. The activity and gene expression of 3b-HSD increased, while the activity and gene expression of 20a-HSD did not show changes with respect to the control. Txf in the ganglion diminished P4 release only at 60 min. E2 plus Txf in the ganglion reverted the effect of E2 alone and the inhibitory effect of Txf. The results of this study demonstrate that ERa activation in the SMG has an impact on ovarian steroidogenesis in rats, thus providing evidence for the critical role of peripheral system neurons in the control of ovarian functions under normal and pathological conditions.
Fil: Vega Orozco, Adriana Soledad. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Medicina y Biología Experimental de Cuyo; Argentina. Universidad Nacional de San Luis. Facultad de Química, Bioquímica y Farmacia. Departamento de Bioquímica y Ciencias Biológicas; Argentina
Fil: Daneri Becerra, Cristina del Rosario. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biología y Medicina Experimental. Fundación de Instituto de Biología y Medicina Experimental. Instituto de Biología y Medicina Experimental; Argentina
Fil: Anesetti, Gabriel. Universidad de la República; Uruguay
Fil: Cabrera Kreiker, Ricardo Jorge. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Medicina y Biología Experimental de Cuyo; Argentina
Fil: Sosa, Zulema Yolanda. Universidad Nacional de San Luis. Facultad de Química, Bioquímica y Farmacia. Departamento de Bioquímica y Ciencias Biológicas; Argentina
Fil: Rastrilla, Ana Maria. Universidad Nacional de San Luis. Facultad de Química, Bioquímica y Farmacia. Departamento de Bioquímica y Ciencias Biológicas; Argentina
Materia
Oestrogenic receptors
Superior mesenteric ganglion
Ovarian steroidogenesis
Female rat
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/274559
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/274559
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Involvement of the oestrogenic receptors in superior mesenteric ganglion on the ovarian steroidogenesis in ratVega Orozco, Adriana SoledadDaneri Becerra, Cristina del RosarioAnesetti, GabrielCabrera Kreiker, Ricardo JorgeSosa, Zulema YolandaRastrilla, Ana MariaOestrogenic receptorsSuperior mesenteric ganglionOvarian steroidogenesisFemale rathttps://purl.org/becyt/ford/3.1https://purl.org/becyt/ford/3Oestradiol (E2) is a key hormone in the regulation of reproductive processes. The aims of this work were a) to examine the distributions of oestrogen receptor a (ERa) and ERb in the neurons of the superior mesenteric ganglion (SMG) in the oestrus stage by immunohistochemistry, b) to demonstrate whether E2 in the SMG modifies progesterone (P4), androstenedione (A2) and nitrite release in the ovarian compartment on oestrus day and c) to demonstrate whether E2 in the ganglion modifies the activity and gene expression in the ovary of the steroidogenic enzymes 3b-hydroxysteroid dehydrogenase (3b-HSD) and 20a-hydroxysteroid dehydrogenase (20a-HSD). The ex vivo SMG–ovarian nervous plexus–ovary system was used. E2, tamoxifen (Txf) and E2 plus Txf were added in the ganglion to measure ovarian P4 release, while E2 alone was added to measure ovarian A2 and nitrites release. Immunohistochemistry revealed cytoplasmic ERa immunoreactivity only in the neural somas in the SMG. E2 increased ovarian P4 and A2 release at 15, 30 and 60 min but decreased nitrites. The activity and gene expression of 3b-HSD increased, while the activity and gene expression of 20a-HSD did not show changes with respect to the control. Txf in the ganglion diminished P4 release only at 60 min. E2 plus Txf in the ganglion reverted the effect of E2 alone and the inhibitory effect of Txf. The results of this study demonstrate that ERa activation in the SMG has an impact on ovarian steroidogenesis in rats, thus providing evidence for the critical role of peripheral system neurons in the control of ovarian functions under normal and pathological conditions.Fil: Vega Orozco, Adriana Soledad. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Medicina y Biología Experimental de Cuyo; Argentina. Universidad Nacional de San Luis. Facultad de Química, Bioquímica y Farmacia. Departamento de Bioquímica y Ciencias Biológicas; ArgentinaFil: Daneri Becerra, Cristina del Rosario. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biología y Medicina Experimental. Fundación de Instituto de Biología y Medicina Experimental. Instituto de Biología y Medicina Experimental; ArgentinaFil: Anesetti, Gabriel. Universidad de la República; UruguayFil: Cabrera Kreiker, Ricardo Jorge. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Medicina y Biología Experimental de Cuyo; ArgentinaFil: Sosa, Zulema Yolanda. Universidad Nacional de San Luis. Facultad de Química, Bioquímica y Farmacia. Departamento de Bioquímica y Ciencias Biológicas; ArgentinaFil: Rastrilla, Ana Maria. Universidad Nacional de San Luis. Facultad de Química, Bioquímica y Farmacia. Departamento de Bioquímica y Ciencias Biológicas; ArgentinaBioScientifica2012-11info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/274559Vega Orozco, Adriana Soledad; Daneri Becerra, Cristina del Rosario; Anesetti, Gabriel; Cabrera Kreiker, Ricardo Jorge; Sosa, Zulema Yolanda; et al.; Involvement of the oestrogenic receptors in superior mesenteric ganglion on the ovarian steroidogenesis in rat; BioScientifica; Reproduction; 143; 2; 11-2012; 183-1931470-1626CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://rep.bioscientifica.com/view/journals/rep/143/2/183.xmlinfo:eu-repo/semantics/altIdentifier/doi/10.1530/REP-11-0056info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-11-05T10:25:18Zoai:ri.conicet.gov.ar:11336/274559instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-11-05 10:25:19.215CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Involvement of the oestrogenic receptors in superior mesenteric ganglion on the ovarian steroidogenesis in rat
title Involvement of the oestrogenic receptors in superior mesenteric ganglion on the ovarian steroidogenesis in rat
spellingShingle Involvement of the oestrogenic receptors in superior mesenteric ganglion on the ovarian steroidogenesis in rat
Vega Orozco, Adriana Soledad
Oestrogenic receptors
Superior mesenteric ganglion
Ovarian steroidogenesis
Female rat
title_short Involvement of the oestrogenic receptors in superior mesenteric ganglion on the ovarian steroidogenesis in rat
title_full Involvement of the oestrogenic receptors in superior mesenteric ganglion on the ovarian steroidogenesis in rat
title_fullStr Involvement of the oestrogenic receptors in superior mesenteric ganglion on the ovarian steroidogenesis in rat
title_full_unstemmed Involvement of the oestrogenic receptors in superior mesenteric ganglion on the ovarian steroidogenesis in rat
title_sort Involvement of the oestrogenic receptors in superior mesenteric ganglion on the ovarian steroidogenesis in rat
dc.creator.none.fl_str_mv Vega Orozco, Adriana Soledad
Daneri Becerra, Cristina del Rosario
Anesetti, Gabriel
Cabrera Kreiker, Ricardo Jorge
Sosa, Zulema Yolanda
Rastrilla, Ana Maria
author Vega Orozco, Adriana Soledad
author_facet Vega Orozco, Adriana Soledad
Daneri Becerra, Cristina del Rosario
Anesetti, Gabriel
Cabrera Kreiker, Ricardo Jorge
Sosa, Zulema Yolanda
Rastrilla, Ana Maria
author_role author
author2 Daneri Becerra, Cristina del Rosario
Anesetti, Gabriel
Cabrera Kreiker, Ricardo Jorge
Sosa, Zulema Yolanda
Rastrilla, Ana Maria
author2_role author
author
author
author
author
dc.subject.none.fl_str_mv Oestrogenic receptors
Superior mesenteric ganglion
Ovarian steroidogenesis
Female rat
topic Oestrogenic receptors
Superior mesenteric ganglion
Ovarian steroidogenesis
Female rat
purl_subject.fl_str_mv https://purl.org/becyt/ford/3.1
https://purl.org/becyt/ford/3
dc.description.none.fl_txt_mv Oestradiol (E2) is a key hormone in the regulation of reproductive processes. The aims of this work were a) to examine the distributions of oestrogen receptor a (ERa) and ERb in the neurons of the superior mesenteric ganglion (SMG) in the oestrus stage by immunohistochemistry, b) to demonstrate whether E2 in the SMG modifies progesterone (P4), androstenedione (A2) and nitrite release in the ovarian compartment on oestrus day and c) to demonstrate whether E2 in the ganglion modifies the activity and gene expression in the ovary of the steroidogenic enzymes 3b-hydroxysteroid dehydrogenase (3b-HSD) and 20a-hydroxysteroid dehydrogenase (20a-HSD). The ex vivo SMG–ovarian nervous plexus–ovary system was used. E2, tamoxifen (Txf) and E2 plus Txf were added in the ganglion to measure ovarian P4 release, while E2 alone was added to measure ovarian A2 and nitrites release. Immunohistochemistry revealed cytoplasmic ERa immunoreactivity only in the neural somas in the SMG. E2 increased ovarian P4 and A2 release at 15, 30 and 60 min but decreased nitrites. The activity and gene expression of 3b-HSD increased, while the activity and gene expression of 20a-HSD did not show changes with respect to the control. Txf in the ganglion diminished P4 release only at 60 min. E2 plus Txf in the ganglion reverted the effect of E2 alone and the inhibitory effect of Txf. The results of this study demonstrate that ERa activation in the SMG has an impact on ovarian steroidogenesis in rats, thus providing evidence for the critical role of peripheral system neurons in the control of ovarian functions under normal and pathological conditions.
Fil: Vega Orozco, Adriana Soledad. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Medicina y Biología Experimental de Cuyo; Argentina. Universidad Nacional de San Luis. Facultad de Química, Bioquímica y Farmacia. Departamento de Bioquímica y Ciencias Biológicas; Argentina
Fil: Daneri Becerra, Cristina del Rosario. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biología y Medicina Experimental. Fundación de Instituto de Biología y Medicina Experimental. Instituto de Biología y Medicina Experimental; Argentina
Fil: Anesetti, Gabriel. Universidad de la República; Uruguay
Fil: Cabrera Kreiker, Ricardo Jorge. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Medicina y Biología Experimental de Cuyo; Argentina
Fil: Sosa, Zulema Yolanda. Universidad Nacional de San Luis. Facultad de Química, Bioquímica y Farmacia. Departamento de Bioquímica y Ciencias Biológicas; Argentina
Fil: Rastrilla, Ana Maria. Universidad Nacional de San Luis. Facultad de Química, Bioquímica y Farmacia. Departamento de Bioquímica y Ciencias Biológicas; Argentina
description Oestradiol (E2) is a key hormone in the regulation of reproductive processes. The aims of this work were a) to examine the distributions of oestrogen receptor a (ERa) and ERb in the neurons of the superior mesenteric ganglion (SMG) in the oestrus stage by immunohistochemistry, b) to demonstrate whether E2 in the SMG modifies progesterone (P4), androstenedione (A2) and nitrite release in the ovarian compartment on oestrus day and c) to demonstrate whether E2 in the ganglion modifies the activity and gene expression in the ovary of the steroidogenic enzymes 3b-hydroxysteroid dehydrogenase (3b-HSD) and 20a-hydroxysteroid dehydrogenase (20a-HSD). The ex vivo SMG–ovarian nervous plexus–ovary system was used. E2, tamoxifen (Txf) and E2 plus Txf were added in the ganglion to measure ovarian P4 release, while E2 alone was added to measure ovarian A2 and nitrites release. Immunohistochemistry revealed cytoplasmic ERa immunoreactivity only in the neural somas in the SMG. E2 increased ovarian P4 and A2 release at 15, 30 and 60 min but decreased nitrites. The activity and gene expression of 3b-HSD increased, while the activity and gene expression of 20a-HSD did not show changes with respect to the control. Txf in the ganglion diminished P4 release only at 60 min. E2 plus Txf in the ganglion reverted the effect of E2 alone and the inhibitory effect of Txf. The results of this study demonstrate that ERa activation in the SMG has an impact on ovarian steroidogenesis in rats, thus providing evidence for the critical role of peripheral system neurons in the control of ovarian functions under normal and pathological conditions.
publishDate 2012
dc.date.none.fl_str_mv 2012-11
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/274559
Vega Orozco, Adriana Soledad; Daneri Becerra, Cristina del Rosario; Anesetti, Gabriel; Cabrera Kreiker, Ricardo Jorge; Sosa, Zulema Yolanda; et al.; Involvement of the oestrogenic receptors in superior mesenteric ganglion on the ovarian steroidogenesis in rat; BioScientifica; Reproduction; 143; 2; 11-2012; 183-193
1470-1626
CONICET Digital
CONICET
url http://hdl.handle.net/11336/274559
identifier_str_mv Vega Orozco, Adriana Soledad; Daneri Becerra, Cristina del Rosario; Anesetti, Gabriel; Cabrera Kreiker, Ricardo Jorge; Sosa, Zulema Yolanda; et al.; Involvement of the oestrogenic receptors in superior mesenteric ganglion on the ovarian steroidogenesis in rat; BioScientifica; Reproduction; 143; 2; 11-2012; 183-193
1470-1626
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://rep.bioscientifica.com/view/journals/rep/143/2/183.xml
info:eu-repo/semantics/altIdentifier/doi/10.1530/REP-11-0056
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv BioScientifica
publisher.none.fl_str_mv BioScientifica
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.087074

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