The heme uptake process in Trypanosoma cruzi epimastigotes is inhibited by heme analogues and by inhibitors of ABC transporters

Autores
Peixoto Cupello, Mauricio; Souza, Cíntia Fernandes de; Buchensky, Celeste; Soares, Juliana Baptista Rocha Corrêa; Laranja, Gustavo Augusto Travasso; Coelho, Marsen Garcia Pinto; Cricco, Julia Alejandra; Paes, Marcia Cristina
Año de publicación
2011
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Heme (iron protoporphyrin IX) is an important molecule involved in many biological reactions, including oxygen transport, respiration, photosynthesis and drug detoxification. Trypanosoma cruzi parasites, the etiological agent of Chagas’ disease, take up heme from the environment to supply their nutritional needs because they do not synthesize this cofactor. However, the mechanisms involved in heme transport across biological membranes are poorly understood. Indeed, in T. cruzi, no heme transporter has yet been characterized. In the present work, we evaluate the heme uptake processes by T. cruzi epimastigotes using fluorescent heme-analogues. Heme uptake decreased significantly when cells were pretreated with different concentrations of SnPPIX, PdMPIX or ZnMPIX, this observed competition suggests that they are taken up by the same transport system. We studied the growth behavior of epimastigotes using the same heme-analogues and the treatments with SnPPIX or PdMPIX impaired cell growth but when heme was added to the culture medium the observed inhibition was partially reversed. In addition, we tested how the heme uptake processes are affected by the presence of different transporter inhibitors. When the cells were treated with inhibitors and then incubated with heme, heme uptake decreased significantly for all treatments. These results constitute a strong indication for the existence of a protein associated with porphyrin transport in T. cruzi, possibly ATP-binding cassette transporters (ABC-transporter).
Fil: Peixoto Cupello, Mauricio. Universidade Do Estado de Rio Do Janeiro; Brasil
Fil: Souza, Cíntia Fernandes de. Universidade Do Estado de Rio Do Janeiro; Brasil
Fil: Buchensky, Celeste. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Rosario. Instituto de Biología Molecular y Celular de Rosario; Argentina. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas; Argentina
Fil: Soares, Juliana Baptista Rocha Corrêa. Universidade Federal do Rio de Janeiro; Brasil
Fil: Laranja, Gustavo Augusto Travasso. Universidade Do Estado de Rio Do Janeiro; Brasil
Fil: Coelho, Marsen Garcia Pinto. Universidade Do Estado de Rio Do Janeiro; Brasil
Fil: Cricco, Julia Alejandra. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Rosario. Instituto de Biología Molecular y Celular de Rosario; Argentina. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas; Argentina
Fil: Paes, Marcia Cristina. Universidade Do Estado de Rio Do Janeiro; Brasil. Instituto Nacional de Ciência e Tecnologia; Brasil
Materia
Heme
Trypanosoma Cruzi
Porphyrins
Heme Transport
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/15449
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/15449
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling The heme uptake process in Trypanosoma cruzi epimastigotes is inhibited by heme analogues and by inhibitors of ABC transportersPeixoto Cupello, MauricioSouza, Cíntia Fernandes deBuchensky, CelesteSoares, Juliana Baptista Rocha CorrêaLaranja, Gustavo Augusto TravassoCoelho, Marsen Garcia PintoCricco, Julia AlejandraPaes, Marcia CristinaHemeTrypanosoma CruziPorphyrinsHeme Transporthttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1Heme (iron protoporphyrin IX) is an important molecule involved in many biological reactions, including oxygen transport, respiration, photosynthesis and drug detoxification. Trypanosoma cruzi parasites, the etiological agent of Chagas’ disease, take up heme from the environment to supply their nutritional needs because they do not synthesize this cofactor. However, the mechanisms involved in heme transport across biological membranes are poorly understood. Indeed, in T. cruzi, no heme transporter has yet been characterized. In the present work, we evaluate the heme uptake processes by T. cruzi epimastigotes using fluorescent heme-analogues. Heme uptake decreased significantly when cells were pretreated with different concentrations of SnPPIX, PdMPIX or ZnMPIX, this observed competition suggests that they are taken up by the same transport system. We studied the growth behavior of epimastigotes using the same heme-analogues and the treatments with SnPPIX or PdMPIX impaired cell growth but when heme was added to the culture medium the observed inhibition was partially reversed. In addition, we tested how the heme uptake processes are affected by the presence of different transporter inhibitors. When the cells were treated with inhibitors and then incubated with heme, heme uptake decreased significantly for all treatments. These results constitute a strong indication for the existence of a protein associated with porphyrin transport in T. cruzi, possibly ATP-binding cassette transporters (ABC-transporter).Fil: Peixoto Cupello, Mauricio. Universidade Do Estado de Rio Do Janeiro; BrasilFil: Souza, Cíntia Fernandes de. Universidade Do Estado de Rio Do Janeiro; BrasilFil: Buchensky, Celeste. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Rosario. Instituto de Biología Molecular y Celular de Rosario; Argentina. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas; ArgentinaFil: Soares, Juliana Baptista Rocha Corrêa. Universidade Federal do Rio de Janeiro; BrasilFil: Laranja, Gustavo Augusto Travasso. Universidade Do Estado de Rio Do Janeiro; BrasilFil: Coelho, Marsen Garcia Pinto. Universidade Do Estado de Rio Do Janeiro; BrasilFil: Cricco, Julia Alejandra. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Rosario. Instituto de Biología Molecular y Celular de Rosario; Argentina. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas; ArgentinaFil: Paes, Marcia Cristina. Universidade Do Estado de Rio Do Janeiro; Brasil. Instituto Nacional de Ciência e Tecnologia; BrasilElsevier Science2011-12info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/15449Peixoto Cupello, Mauricio; Souza, Cíntia Fernandes de; Buchensky, Celeste; Soares, Juliana Baptista Rocha Corrêa; Laranja, Gustavo Augusto Travasso; et al.; The heme uptake process in Trypanosoma cruzi epimastigotes is inhibited by heme analogues and by inhibitors of ABC transporters; Elsevier Science; Acta Tropica; 120; 3; 12-2011; 211-2180001-706Xenginfo:eu-repo/semantics/altIdentifier/doi/10.1016/j.actatropica.2011.08.011info:eu-repo/semantics/altIdentifier/url/http://www.sciencedirect.com/science/article/pii/S0001706X11002531info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-nd/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T09:33:16Zoai:ri.conicet.gov.ar:11336/15449instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 09:33:16.958CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv The heme uptake process in Trypanosoma cruzi epimastigotes is inhibited by heme analogues and by inhibitors of ABC transporters
title The heme uptake process in Trypanosoma cruzi epimastigotes is inhibited by heme analogues and by inhibitors of ABC transporters
spellingShingle The heme uptake process in Trypanosoma cruzi epimastigotes is inhibited by heme analogues and by inhibitors of ABC transporters
Peixoto Cupello, Mauricio
Heme
Trypanosoma Cruzi
Porphyrins
Heme Transport
title_short The heme uptake process in Trypanosoma cruzi epimastigotes is inhibited by heme analogues and by inhibitors of ABC transporters
title_full The heme uptake process in Trypanosoma cruzi epimastigotes is inhibited by heme analogues and by inhibitors of ABC transporters
title_fullStr The heme uptake process in Trypanosoma cruzi epimastigotes is inhibited by heme analogues and by inhibitors of ABC transporters
title_full_unstemmed The heme uptake process in Trypanosoma cruzi epimastigotes is inhibited by heme analogues and by inhibitors of ABC transporters
title_sort The heme uptake process in Trypanosoma cruzi epimastigotes is inhibited by heme analogues and by inhibitors of ABC transporters
dc.creator.none.fl_str_mv Peixoto Cupello, Mauricio
Souza, Cíntia Fernandes de
Buchensky, Celeste
Soares, Juliana Baptista Rocha Corrêa
Laranja, Gustavo Augusto Travasso
Coelho, Marsen Garcia Pinto
Cricco, Julia Alejandra
Paes, Marcia Cristina
author Peixoto Cupello, Mauricio
author_facet Peixoto Cupello, Mauricio
Souza, Cíntia Fernandes de
Buchensky, Celeste
Soares, Juliana Baptista Rocha Corrêa
Laranja, Gustavo Augusto Travasso
Coelho, Marsen Garcia Pinto
Cricco, Julia Alejandra
Paes, Marcia Cristina
author_role author
author2 Souza, Cíntia Fernandes de
Buchensky, Celeste
Soares, Juliana Baptista Rocha Corrêa
Laranja, Gustavo Augusto Travasso
Coelho, Marsen Garcia Pinto
Cricco, Julia Alejandra
Paes, Marcia Cristina
author2_role author
author
author
author
author
author
author
dc.subject.none.fl_str_mv Heme
Trypanosoma Cruzi
Porphyrins
Heme Transport
topic Heme
Trypanosoma Cruzi
Porphyrins
Heme Transport
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv Heme (iron protoporphyrin IX) is an important molecule involved in many biological reactions, including oxygen transport, respiration, photosynthesis and drug detoxification. Trypanosoma cruzi parasites, the etiological agent of Chagas’ disease, take up heme from the environment to supply their nutritional needs because they do not synthesize this cofactor. However, the mechanisms involved in heme transport across biological membranes are poorly understood. Indeed, in T. cruzi, no heme transporter has yet been characterized. In the present work, we evaluate the heme uptake processes by T. cruzi epimastigotes using fluorescent heme-analogues. Heme uptake decreased significantly when cells were pretreated with different concentrations of SnPPIX, PdMPIX or ZnMPIX, this observed competition suggests that they are taken up by the same transport system. We studied the growth behavior of epimastigotes using the same heme-analogues and the treatments with SnPPIX or PdMPIX impaired cell growth but when heme was added to the culture medium the observed inhibition was partially reversed. In addition, we tested how the heme uptake processes are affected by the presence of different transporter inhibitors. When the cells were treated with inhibitors and then incubated with heme, heme uptake decreased significantly for all treatments. These results constitute a strong indication for the existence of a protein associated with porphyrin transport in T. cruzi, possibly ATP-binding cassette transporters (ABC-transporter).
Fil: Peixoto Cupello, Mauricio. Universidade Do Estado de Rio Do Janeiro; Brasil
Fil: Souza, Cíntia Fernandes de. Universidade Do Estado de Rio Do Janeiro; Brasil
Fil: Buchensky, Celeste. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Rosario. Instituto de Biología Molecular y Celular de Rosario; Argentina. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas; Argentina
Fil: Soares, Juliana Baptista Rocha Corrêa. Universidade Federal do Rio de Janeiro; Brasil
Fil: Laranja, Gustavo Augusto Travasso. Universidade Do Estado de Rio Do Janeiro; Brasil
Fil: Coelho, Marsen Garcia Pinto. Universidade Do Estado de Rio Do Janeiro; Brasil
Fil: Cricco, Julia Alejandra. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Rosario. Instituto de Biología Molecular y Celular de Rosario; Argentina. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas; Argentina
Fil: Paes, Marcia Cristina. Universidade Do Estado de Rio Do Janeiro; Brasil. Instituto Nacional de Ciência e Tecnologia; Brasil
description Heme (iron protoporphyrin IX) is an important molecule involved in many biological reactions, including oxygen transport, respiration, photosynthesis and drug detoxification. Trypanosoma cruzi parasites, the etiological agent of Chagas’ disease, take up heme from the environment to supply their nutritional needs because they do not synthesize this cofactor. However, the mechanisms involved in heme transport across biological membranes are poorly understood. Indeed, in T. cruzi, no heme transporter has yet been characterized. In the present work, we evaluate the heme uptake processes by T. cruzi epimastigotes using fluorescent heme-analogues. Heme uptake decreased significantly when cells were pretreated with different concentrations of SnPPIX, PdMPIX or ZnMPIX, this observed competition suggests that they are taken up by the same transport system. We studied the growth behavior of epimastigotes using the same heme-analogues and the treatments with SnPPIX or PdMPIX impaired cell growth but when heme was added to the culture medium the observed inhibition was partially reversed. In addition, we tested how the heme uptake processes are affected by the presence of different transporter inhibitors. When the cells were treated with inhibitors and then incubated with heme, heme uptake decreased significantly for all treatments. These results constitute a strong indication for the existence of a protein associated with porphyrin transport in T. cruzi, possibly ATP-binding cassette transporters (ABC-transporter).
publishDate 2011
dc.date.none.fl_str_mv 2011-12
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/15449
Peixoto Cupello, Mauricio; Souza, Cíntia Fernandes de; Buchensky, Celeste; Soares, Juliana Baptista Rocha Corrêa; Laranja, Gustavo Augusto Travasso; et al.; The heme uptake process in Trypanosoma cruzi epimastigotes is inhibited by heme analogues and by inhibitors of ABC transporters; Elsevier Science; Acta Tropica; 120; 3; 12-2011; 211-218
0001-706X
url http://hdl.handle.net/11336/15449
identifier_str_mv Peixoto Cupello, Mauricio; Souza, Cíntia Fernandes de; Buchensky, Celeste; Soares, Juliana Baptista Rocha Corrêa; Laranja, Gustavo Augusto Travasso; et al.; The heme uptake process in Trypanosoma cruzi epimastigotes is inhibited by heme analogues and by inhibitors of ABC transporters; Elsevier Science; Acta Tropica; 120; 3; 12-2011; 211-218
0001-706X
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.1016/j.actatropica.2011.08.011
info:eu-repo/semantics/altIdentifier/url/http://www.sciencedirect.com/science/article/pii/S0001706X11002531
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Elsevier Science
publisher.none.fl_str_mv Elsevier Science
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.070432

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