Discrimination of four Culex (Culex) species from the Neotropics based on geometric morphometrics

Autores
Laurito, Magdalena; Almiron, Walter Ricardo; Ludueña Almeida, Francisco
Año de publicación
2015
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Achieving correct identification of Culex species is difficult because many anatomical characters of larvae and females are polymorphic or overlap among distinct species. The overlapping is known to occur between Culex bidens, Cx. interfor, Cx. mollis and Cx. tatoi, from the subgenus Culex. The first three were incriminated in viruses’ transmission in Argentina. The purpose was to distinguish between specimens of four species using geometric morphometric procedures. From field and entomological collections, 10 type I and 10 type II landmarks on the wings of females and the dorsomentum of larvae, respectively, were defined. The free morphometric software modules by J.P. Dujardin were used. Landmark coordinates were submitted to Procrustes and TPS analyses to generate size (centroid size, CS) and shape (partial warps, PW) variables. Size analysis was performed by nonparametric comparisons of CS measurements based on permutations, and shape, by submission of PW to discriminant analysis. Re-identification and identification of external specimens were also realized. Wing and Dm shapes gave similar results. The individuals arranged into three groups coinciding with Cx. bidens + Cx. interfor, Cx. mollis and Cx. tatoi. The latter two had a very different wing and Dm shapes. The accurate re-identification was greater than or equal to 77 % in all cases, and the identification of external specimens was achieved for all the species and both structures. Larval characters are more informative than female features. Geometric morphometrics, as a complementary tool, will facilitate identification in junction with others, such as DNA sequence analysis.
Fil: Laurito, Magdalena. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Investigaciones Biológicas y Tecnológicas. Universidad Nacional de Córdoba. Facultad de Ciencias Exactas, Físicas y Naturales. Instituto de Investigaciones Biológicas y Tecnológicas; Argentina
Fil: Almiron, Walter Ricardo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Investigaciones Biológicas y Tecnológicas. Universidad Nacional de Córdoba. Facultad de Ciencias Exactas, Físicas y Naturales. Instituto de Investigaciones Biológicas y Tecnológicas; Argentina
Fil: Ludueña Almeida, Francisco. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Investigaciones Biológicas y Tecnológicas. Universidad Nacional de Córdoba. Facultad de Ciencias Exactas, Físicas y Naturales. Instituto de Investigaciones Biológicas y Tecnológicas; Argentina
Materia
Identification
Mosquito
Shape
Size
Taxonomy
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/38348
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/38348
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network_name_str CONICET Digital (CONICET)
spelling Discrimination of four Culex (Culex) species from the Neotropics based on geometric morphometricsLaurito, MagdalenaAlmiron, Walter RicardoLudueña Almeida, FranciscoIdentificationMosquitoShapeSizeTaxonomyhttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1Achieving correct identification of Culex species is difficult because many anatomical characters of larvae and females are polymorphic or overlap among distinct species. The overlapping is known to occur between Culex bidens, Cx. interfor, Cx. mollis and Cx. tatoi, from the subgenus Culex. The first three were incriminated in viruses’ transmission in Argentina. The purpose was to distinguish between specimens of four species using geometric morphometric procedures. From field and entomological collections, 10 type I and 10 type II landmarks on the wings of females and the dorsomentum of larvae, respectively, were defined. The free morphometric software modules by J.P. Dujardin were used. Landmark coordinates were submitted to Procrustes and TPS analyses to generate size (centroid size, CS) and shape (partial warps, PW) variables. Size analysis was performed by nonparametric comparisons of CS measurements based on permutations, and shape, by submission of PW to discriminant analysis. Re-identification and identification of external specimens were also realized. Wing and Dm shapes gave similar results. The individuals arranged into three groups coinciding with Cx. bidens + Cx. interfor, Cx. mollis and Cx. tatoi. The latter two had a very different wing and Dm shapes. The accurate re-identification was greater than or equal to 77 % in all cases, and the identification of external specimens was achieved for all the species and both structures. Larval characters are more informative than female features. Geometric morphometrics, as a complementary tool, will facilitate identification in junction with others, such as DNA sequence analysis.Fil: Laurito, Magdalena. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Investigaciones Biológicas y Tecnológicas. Universidad Nacional de Córdoba. Facultad de Ciencias Exactas, Físicas y Naturales. Instituto de Investigaciones Biológicas y Tecnológicas; ArgentinaFil: Almiron, Walter Ricardo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Investigaciones Biológicas y Tecnológicas. Universidad Nacional de Córdoba. Facultad de Ciencias Exactas, Físicas y Naturales. Instituto de Investigaciones Biológicas y Tecnológicas; ArgentinaFil: Ludueña Almeida, Francisco. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Investigaciones Biológicas y Tecnológicas. Universidad Nacional de Córdoba. Facultad de Ciencias Exactas, Físicas y Naturales. Instituto de Investigaciones Biológicas y Tecnológicas; ArgentinaSpringer2015-10info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/38348Laurito, Magdalena; Almiron, Walter Ricardo; Ludueña Almeida, Francisco; Discrimination of four Culex (Culex) species from the Neotropics based on geometric morphometrics; Springer; Zoomorphology (Berlin. Print); 134; 3; 10-2015; 447-4550720-213X1432-234XCONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.1007/s00435-015-0271-xinfo:eu-repo/semantics/altIdentifier/url/https://link.springer.com/article/10.1007%2Fs00435-015-0271-xinfo:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T09:56:37Zoai:ri.conicet.gov.ar:11336/38348instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 09:56:37.602CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Discrimination of four Culex (Culex) species from the Neotropics based on geometric morphometrics
title Discrimination of four Culex (Culex) species from the Neotropics based on geometric morphometrics
spellingShingle Discrimination of four Culex (Culex) species from the Neotropics based on geometric morphometrics
Laurito, Magdalena
Identification
Mosquito
Shape
Size
Taxonomy
title_short Discrimination of four Culex (Culex) species from the Neotropics based on geometric morphometrics
title_full Discrimination of four Culex (Culex) species from the Neotropics based on geometric morphometrics
title_fullStr Discrimination of four Culex (Culex) species from the Neotropics based on geometric morphometrics
title_full_unstemmed Discrimination of four Culex (Culex) species from the Neotropics based on geometric morphometrics
title_sort Discrimination of four Culex (Culex) species from the Neotropics based on geometric morphometrics
dc.creator.none.fl_str_mv Laurito, Magdalena
Almiron, Walter Ricardo
Ludueña Almeida, Francisco
author Laurito, Magdalena
author_facet Laurito, Magdalena
Almiron, Walter Ricardo
Ludueña Almeida, Francisco
author_role author
author2 Almiron, Walter Ricardo
Ludueña Almeida, Francisco
author2_role author
author
dc.subject.none.fl_str_mv Identification
Mosquito
Shape
Size
Taxonomy
topic Identification
Mosquito
Shape
Size
Taxonomy
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv Achieving correct identification of Culex species is difficult because many anatomical characters of larvae and females are polymorphic or overlap among distinct species. The overlapping is known to occur between Culex bidens, Cx. interfor, Cx. mollis and Cx. tatoi, from the subgenus Culex. The first three were incriminated in viruses’ transmission in Argentina. The purpose was to distinguish between specimens of four species using geometric morphometric procedures. From field and entomological collections, 10 type I and 10 type II landmarks on the wings of females and the dorsomentum of larvae, respectively, were defined. The free morphometric software modules by J.P. Dujardin were used. Landmark coordinates were submitted to Procrustes and TPS analyses to generate size (centroid size, CS) and shape (partial warps, PW) variables. Size analysis was performed by nonparametric comparisons of CS measurements based on permutations, and shape, by submission of PW to discriminant analysis. Re-identification and identification of external specimens were also realized. Wing and Dm shapes gave similar results. The individuals arranged into three groups coinciding with Cx. bidens + Cx. interfor, Cx. mollis and Cx. tatoi. The latter two had a very different wing and Dm shapes. The accurate re-identification was greater than or equal to 77 % in all cases, and the identification of external specimens was achieved for all the species and both structures. Larval characters are more informative than female features. Geometric morphometrics, as a complementary tool, will facilitate identification in junction with others, such as DNA sequence analysis.
Fil: Laurito, Magdalena. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Investigaciones Biológicas y Tecnológicas. Universidad Nacional de Córdoba. Facultad de Ciencias Exactas, Físicas y Naturales. Instituto de Investigaciones Biológicas y Tecnológicas; Argentina
Fil: Almiron, Walter Ricardo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Investigaciones Biológicas y Tecnológicas. Universidad Nacional de Córdoba. Facultad de Ciencias Exactas, Físicas y Naturales. Instituto de Investigaciones Biológicas y Tecnológicas; Argentina
Fil: Ludueña Almeida, Francisco. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Investigaciones Biológicas y Tecnológicas. Universidad Nacional de Córdoba. Facultad de Ciencias Exactas, Físicas y Naturales. Instituto de Investigaciones Biológicas y Tecnológicas; Argentina
description Achieving correct identification of Culex species is difficult because many anatomical characters of larvae and females are polymorphic or overlap among distinct species. The overlapping is known to occur between Culex bidens, Cx. interfor, Cx. mollis and Cx. tatoi, from the subgenus Culex. The first three were incriminated in viruses’ transmission in Argentina. The purpose was to distinguish between specimens of four species using geometric morphometric procedures. From field and entomological collections, 10 type I and 10 type II landmarks on the wings of females and the dorsomentum of larvae, respectively, were defined. The free morphometric software modules by J.P. Dujardin were used. Landmark coordinates were submitted to Procrustes and TPS analyses to generate size (centroid size, CS) and shape (partial warps, PW) variables. Size analysis was performed by nonparametric comparisons of CS measurements based on permutations, and shape, by submission of PW to discriminant analysis. Re-identification and identification of external specimens were also realized. Wing and Dm shapes gave similar results. The individuals arranged into three groups coinciding with Cx. bidens + Cx. interfor, Cx. mollis and Cx. tatoi. The latter two had a very different wing and Dm shapes. The accurate re-identification was greater than or equal to 77 % in all cases, and the identification of external specimens was achieved for all the species and both structures. Larval characters are more informative than female features. Geometric morphometrics, as a complementary tool, will facilitate identification in junction with others, such as DNA sequence analysis.
publishDate 2015
dc.date.none.fl_str_mv 2015-10
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/38348
Laurito, Magdalena; Almiron, Walter Ricardo; Ludueña Almeida, Francisco; Discrimination of four Culex (Culex) species from the Neotropics based on geometric morphometrics; Springer; Zoomorphology (Berlin. Print); 134; 3; 10-2015; 447-455
0720-213X
1432-234X
CONICET Digital
CONICET
url http://hdl.handle.net/11336/38348
identifier_str_mv Laurito, Magdalena; Almiron, Walter Ricardo; Ludueña Almeida, Francisco; Discrimination of four Culex (Culex) species from the Neotropics based on geometric morphometrics; Springer; Zoomorphology (Berlin. Print); 134; 3; 10-2015; 447-455
0720-213X
1432-234X
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.1007/s00435-015-0271-x
info:eu-repo/semantics/altIdentifier/url/https://link.springer.com/article/10.1007%2Fs00435-015-0271-x
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Springer
publisher.none.fl_str_mv Springer
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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