Wing geometric morphometrics as a tool for the identification of Culex subgenus mosquitoes of Culex (Diptera: Culicidae)

Autores
Simões, Roseli; Wilke, André; Romeiro Fernandes Chagas, Carolina; Tironi de Menezes, Regiane; Suesdek, Lincoln; Multini, Laura; Santos Silva, Fabiana; Grech, Marta Gladys; Toledo Marrelli, Mauro; Kirchgatter, Karin
Año de publicación
2020
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Culex is the largest subgenus within the genus Culex that includes important vectors of diseases. The correct identification of mosquitoes is critical for effective control strategies. Wing geometric morphometrics (WGM) has been used to identify mosquito species alongside traditional identification methods. Here, WGM was used for eleven Culex species from São Paulo, Brazil, and one from Esquel, Argentina. Adult mosquitoes were collected using CDC (Centers for Disease Control) traps, morphologically identified and analyzed by WGM. The canonical variate analysis (CVA) was performed and a Neighbor-joining (NJ) tree was constructed to illustrate the patterns of species segregation. A cross-validated reclassification test was also carried out.From 110 comparisons in the cross-validated reclassification test, 87 yielded values higher than 70%, with 13 comparisons yielding 100% reclassification scores. Culex quinquefasciatus yielded the highest reclassification scores among the analyzed species, corroborating with the results obtained by the CVA, in which Cx. quinquefasciatus was the most distinct species. The high values obtained at the cross-validated reclassification test and in the NJ analysis as well as the segregation observed at the CVA made it possible to distinguish among Culex species with high degrees of confidence, suggesting that WGM is a reliable tool to identify Culex species of the subgenus Culex.
Fil: Simões, Roseli. Universidade de Sao Paulo; Brasil
Fil: Wilke, André. University of Miami; Estados Unidos
Fil: Romeiro Fernandes Chagas, Carolina. Nature Research Centre.Institute of Ecology; Lituania
Fil: Tironi de Menezes, Regiane. Superintendência Controle de Endemias do Estado de Sao Paulo; Brasil
Fil: Suesdek, Lincoln. Universidade de Sao Paulo; Brasil
Fil: Multini, Laura. Universidade de Sao Paulo; Brasil
Fil: Santos Silva, Fabiana. Universidade de Sao Paulo; Brasil. Superintendência Controle de Endemias do Estado de Sao Paulo; Brasil
Fil: Grech, Marta Gladys. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Patagonia Norte. Centro de Investigación Esquel de Montaña y Estepa Patagóica. Universidad Nacional de la Patagonia "San Juan Bosco". Facultad de Ciencias Naturales - Sede Esquel. Centro de Investigación Esquel de Montaña y Estepa Patagónica; Argentina. Superintendência Controle de Endemias do Estado de Sao Paulo; Brasil
Fil: Toledo Marrelli, Mauro. Universidade de Sao Paulo; Brasil
Fil: Kirchgatter, Karin. Superintendência Controle de Endemias do Estado de Sao Paulo; Brasil. Universidade de Sao Paulo; Brasil
Materia
CULEX
MOSQUITOES
MORPHOMETRY
ATALANTIC FOREST
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/113447
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/113447
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Wing geometric morphometrics as a tool for the identification of Culex subgenus mosquitoes of Culex (Diptera: Culicidae)Simões, RoseliWilke, AndréRomeiro Fernandes Chagas, CarolinaTironi de Menezes, RegianeSuesdek, LincolnMultini, LauraSantos Silva, FabianaGrech, Marta GladysToledo Marrelli, MauroKirchgatter, KarinCULEXMOSQUITOESMORPHOMETRYATALANTIC FORESThttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1Culex is the largest subgenus within the genus Culex that includes important vectors of diseases. The correct identification of mosquitoes is critical for effective control strategies. Wing geometric morphometrics (WGM) has been used to identify mosquito species alongside traditional identification methods. Here, WGM was used for eleven Culex species from São Paulo, Brazil, and one from Esquel, Argentina. Adult mosquitoes were collected using CDC (Centers for Disease Control) traps, morphologically identified and analyzed by WGM. The canonical variate analysis (CVA) was performed and a Neighbor-joining (NJ) tree was constructed to illustrate the patterns of species segregation. A cross-validated reclassification test was also carried out.From 110 comparisons in the cross-validated reclassification test, 87 yielded values higher than 70%, with 13 comparisons yielding 100% reclassification scores. Culex quinquefasciatus yielded the highest reclassification scores among the analyzed species, corroborating with the results obtained by the CVA, in which Cx. quinquefasciatus was the most distinct species. The high values obtained at the cross-validated reclassification test and in the NJ analysis as well as the segregation observed at the CVA made it possible to distinguish among Culex species with high degrees of confidence, suggesting that WGM is a reliable tool to identify Culex species of the subgenus Culex.Fil: Simões, Roseli. Universidade de Sao Paulo; BrasilFil: Wilke, André. University of Miami; Estados UnidosFil: Romeiro Fernandes Chagas, Carolina. Nature Research Centre.Institute of Ecology; LituaniaFil: Tironi de Menezes, Regiane. Superintendência Controle de Endemias do Estado de Sao Paulo; BrasilFil: Suesdek, Lincoln. Universidade de Sao Paulo; BrasilFil: Multini, Laura. Universidade de Sao Paulo; BrasilFil: Santos Silva, Fabiana. Universidade de Sao Paulo; Brasil. Superintendência Controle de Endemias do Estado de Sao Paulo; BrasilFil: Grech, Marta Gladys. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Patagonia Norte. Centro de Investigación Esquel de Montaña y Estepa Patagóica. Universidad Nacional de la Patagonia "San Juan Bosco". Facultad de Ciencias Naturales - Sede Esquel. Centro de Investigación Esquel de Montaña y Estepa Patagónica; Argentina. Superintendência Controle de Endemias do Estado de Sao Paulo; BrasilFil: Toledo Marrelli, Mauro. Universidade de Sao Paulo; BrasilFil: Kirchgatter, Karin. Superintendência Controle de Endemias do Estado de Sao Paulo; Brasil. Universidade de Sao Paulo; BrasilMolecular Diversity Preservation International2020-08info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/113447Simões, Roseli; Wilke, André; Romeiro Fernandes Chagas, Carolina; Tironi de Menezes, Regiane ; Suesdek, Lincoln; et al.; Wing geometric morphometrics as a tool for the identification of Culex subgenus mosquitoes of Culex (Diptera: Culicidae); Molecular Diversity Preservation International; Insects; 11; 9; 8-2020; 1-132075-4450CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://www.mdpi.com/2075-4450/11/9/567info:eu-repo/semantics/altIdentifier/doi/10.3390/insects11090567info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-03T09:53:15Zoai:ri.conicet.gov.ar:11336/113447instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-03 09:53:15.792CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Wing geometric morphometrics as a tool for the identification of Culex subgenus mosquitoes of Culex (Diptera: Culicidae)
title Wing geometric morphometrics as a tool for the identification of Culex subgenus mosquitoes of Culex (Diptera: Culicidae)
spellingShingle Wing geometric morphometrics as a tool for the identification of Culex subgenus mosquitoes of Culex (Diptera: Culicidae)
Simões, Roseli
CULEX
MOSQUITOES
MORPHOMETRY
ATALANTIC FOREST
title_short Wing geometric morphometrics as a tool for the identification of Culex subgenus mosquitoes of Culex (Diptera: Culicidae)
title_full Wing geometric morphometrics as a tool for the identification of Culex subgenus mosquitoes of Culex (Diptera: Culicidae)
title_fullStr Wing geometric morphometrics as a tool for the identification of Culex subgenus mosquitoes of Culex (Diptera: Culicidae)
title_full_unstemmed Wing geometric morphometrics as a tool for the identification of Culex subgenus mosquitoes of Culex (Diptera: Culicidae)
title_sort Wing geometric morphometrics as a tool for the identification of Culex subgenus mosquitoes of Culex (Diptera: Culicidae)
dc.creator.none.fl_str_mv Simões, Roseli
Wilke, André
Romeiro Fernandes Chagas, Carolina
Tironi de Menezes, Regiane
Suesdek, Lincoln
Multini, Laura
Santos Silva, Fabiana
Grech, Marta Gladys
Toledo Marrelli, Mauro
Kirchgatter, Karin
author Simões, Roseli
author_facet Simões, Roseli
Wilke, André
Romeiro Fernandes Chagas, Carolina
Tironi de Menezes, Regiane
Suesdek, Lincoln
Multini, Laura
Santos Silva, Fabiana
Grech, Marta Gladys
Toledo Marrelli, Mauro
Kirchgatter, Karin
author_role author
author2 Wilke, André
Romeiro Fernandes Chagas, Carolina
Tironi de Menezes, Regiane
Suesdek, Lincoln
Multini, Laura
Santos Silva, Fabiana
Grech, Marta Gladys
Toledo Marrelli, Mauro
Kirchgatter, Karin
author2_role author
author
author
author
author
author
author
author
author
dc.subject.none.fl_str_mv CULEX
MOSQUITOES
MORPHOMETRY
ATALANTIC FOREST
topic CULEX
MOSQUITOES
MORPHOMETRY
ATALANTIC FOREST
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv Culex is the largest subgenus within the genus Culex that includes important vectors of diseases. The correct identification of mosquitoes is critical for effective control strategies. Wing geometric morphometrics (WGM) has been used to identify mosquito species alongside traditional identification methods. Here, WGM was used for eleven Culex species from São Paulo, Brazil, and one from Esquel, Argentina. Adult mosquitoes were collected using CDC (Centers for Disease Control) traps, morphologically identified and analyzed by WGM. The canonical variate analysis (CVA) was performed and a Neighbor-joining (NJ) tree was constructed to illustrate the patterns of species segregation. A cross-validated reclassification test was also carried out.From 110 comparisons in the cross-validated reclassification test, 87 yielded values higher than 70%, with 13 comparisons yielding 100% reclassification scores. Culex quinquefasciatus yielded the highest reclassification scores among the analyzed species, corroborating with the results obtained by the CVA, in which Cx. quinquefasciatus was the most distinct species. The high values obtained at the cross-validated reclassification test and in the NJ analysis as well as the segregation observed at the CVA made it possible to distinguish among Culex species with high degrees of confidence, suggesting that WGM is a reliable tool to identify Culex species of the subgenus Culex.
Fil: Simões, Roseli. Universidade de Sao Paulo; Brasil
Fil: Wilke, André. University of Miami; Estados Unidos
Fil: Romeiro Fernandes Chagas, Carolina. Nature Research Centre.Institute of Ecology; Lituania
Fil: Tironi de Menezes, Regiane. Superintendência Controle de Endemias do Estado de Sao Paulo; Brasil
Fil: Suesdek, Lincoln. Universidade de Sao Paulo; Brasil
Fil: Multini, Laura. Universidade de Sao Paulo; Brasil
Fil: Santos Silva, Fabiana. Universidade de Sao Paulo; Brasil. Superintendência Controle de Endemias do Estado de Sao Paulo; Brasil
Fil: Grech, Marta Gladys. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Patagonia Norte. Centro de Investigación Esquel de Montaña y Estepa Patagóica. Universidad Nacional de la Patagonia "San Juan Bosco". Facultad de Ciencias Naturales - Sede Esquel. Centro de Investigación Esquel de Montaña y Estepa Patagónica; Argentina. Superintendência Controle de Endemias do Estado de Sao Paulo; Brasil
Fil: Toledo Marrelli, Mauro. Universidade de Sao Paulo; Brasil
Fil: Kirchgatter, Karin. Superintendência Controle de Endemias do Estado de Sao Paulo; Brasil. Universidade de Sao Paulo; Brasil
description Culex is the largest subgenus within the genus Culex that includes important vectors of diseases. The correct identification of mosquitoes is critical for effective control strategies. Wing geometric morphometrics (WGM) has been used to identify mosquito species alongside traditional identification methods. Here, WGM was used for eleven Culex species from São Paulo, Brazil, and one from Esquel, Argentina. Adult mosquitoes were collected using CDC (Centers for Disease Control) traps, morphologically identified and analyzed by WGM. The canonical variate analysis (CVA) was performed and a Neighbor-joining (NJ) tree was constructed to illustrate the patterns of species segregation. A cross-validated reclassification test was also carried out.From 110 comparisons in the cross-validated reclassification test, 87 yielded values higher than 70%, with 13 comparisons yielding 100% reclassification scores. Culex quinquefasciatus yielded the highest reclassification scores among the analyzed species, corroborating with the results obtained by the CVA, in which Cx. quinquefasciatus was the most distinct species. The high values obtained at the cross-validated reclassification test and in the NJ analysis as well as the segregation observed at the CVA made it possible to distinguish among Culex species with high degrees of confidence, suggesting that WGM is a reliable tool to identify Culex species of the subgenus Culex.
publishDate 2020
dc.date.none.fl_str_mv 2020-08
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/113447
Simões, Roseli; Wilke, André; Romeiro Fernandes Chagas, Carolina; Tironi de Menezes, Regiane ; Suesdek, Lincoln; et al.; Wing geometric morphometrics as a tool for the identification of Culex subgenus mosquitoes of Culex (Diptera: Culicidae); Molecular Diversity Preservation International; Insects; 11; 9; 8-2020; 1-13
2075-4450
CONICET Digital
CONICET
url http://hdl.handle.net/11336/113447
identifier_str_mv Simões, Roseli; Wilke, André; Romeiro Fernandes Chagas, Carolina; Tironi de Menezes, Regiane ; Suesdek, Lincoln; et al.; Wing geometric morphometrics as a tool for the identification of Culex subgenus mosquitoes of Culex (Diptera: Culicidae); Molecular Diversity Preservation International; Insects; 11; 9; 8-2020; 1-13
2075-4450
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://www.mdpi.com/2075-4450/11/9/567
info:eu-repo/semantics/altIdentifier/doi/10.3390/insects11090567
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Molecular Diversity Preservation International
publisher.none.fl_str_mv Molecular Diversity Preservation International
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.13397

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