Diclofenac induces basophil degranulation without increasing CD63 expression in sensitive patients

Autores
Malbrán, Alejandro; Yeyati, E.; Rey, G. L.; Galassi, Nora Virginia
Año de publicación
2007
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Diclofenac (Dc) induces an IgE-independent basophil (Ba) degranulation in susceptible individuals. CD63 Ba expression is utilized as an in vitro test for diagnosis of drug hypersensitivity. We tested the ability of Dc to induce CD63 Ba expression by flow cytometry (BAT) and Ba degranulation using light microscopy (HBDT) in patients sensitive to Dc. We studied 14 patients with diclofenac hypersensitivity, also two patients sensitive to Dermatophagoides pteronyssinus (Dp), and 12 normal controls. HBDT was performed by mononuclear cells toluidine blue staining. BAT determined CD63 expression in antiCD63/anti-IgE/anti-CD45-labelled whole blood. In each case, the percentage of activated Ba post-stimulation with 1 and 10 μg/ml Dc was determined. Positive controls included N-formyl-methionyl-leucyl-phenylalanine (fMLP) peptide-induced activation. IgE-mediated Ba activation was induced with a Dp allergenic extract. With Dc 1 μg/ml, mean HBDT in Dc-susceptible individuals was 33.62 ± 18.35% and 8.49 ± 4.79% in controls (P = 0.0001). Mean BAT was 2.04 ± 1.68% and 1.93 ± 1.40% in controls (P = 0.8). Ba preincubation with Dc did not affect fMLP-induced CD63 expression, neither in Dc-sensitive individuals (P = 0.8) (n = 4) nor in subjects without Dc hypersensitivity (P = 0.25) (n = 4). Ba from the two patients sensitive both to Dc and Dp responded to Dp but not to Dc by BAT: Dc, 1.99 ± 0.78%; Dp: 60.87 ± 9.28%; but showed degranulation by HBDT: Dc, 30.53 ± 1.02%, Dp: 48.78 ± 22.17%. Dc induces Ba degranulation in sensitive patients in a way that does not induce CD63 expression and is different from IgE-mediated and fMLP-mediated degranulation. Our results suggest that CD63 expression is not a reliable diagnostic method for diclofenac allergy. © 2006 The Author(s).
Fil: Malbrán, Alejandro. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Hospital Británico de Buenos Aires; Argentina
Fil: Yeyati, E.. Hospital Británico de Buenos Aires; Argentina
Fil: Rey, G. L.. Hospital Británico de Buenos Aires; Argentina
Fil: Galassi, Nora Virginia. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Academia Nacional de Medicina de Buenos Aires; Argentina
Materia
Allergy
Basophil
Cd63
Exocytosis
Nsaids
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/56438
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/56438
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Diclofenac induces basophil degranulation without increasing CD63 expression in sensitive patientsMalbrán, AlejandroYeyati, E.Rey, G. L.Galassi, Nora VirginiaAllergyBasophilCd63ExocytosisNsaidshttps://purl.org/becyt/ford/3.2https://purl.org/becyt/ford/3Diclofenac (Dc) induces an IgE-independent basophil (Ba) degranulation in susceptible individuals. CD63 Ba expression is utilized as an in vitro test for diagnosis of drug hypersensitivity. We tested the ability of Dc to induce CD63 Ba expression by flow cytometry (BAT) and Ba degranulation using light microscopy (HBDT) in patients sensitive to Dc. We studied 14 patients with diclofenac hypersensitivity, also two patients sensitive to Dermatophagoides pteronyssinus (Dp), and 12 normal controls. HBDT was performed by mononuclear cells toluidine blue staining. BAT determined CD63 expression in antiCD63/anti-IgE/anti-CD45-labelled whole blood. In each case, the percentage of activated Ba post-stimulation with 1 and 10 μg/ml Dc was determined. Positive controls included N-formyl-methionyl-leucyl-phenylalanine (fMLP) peptide-induced activation. IgE-mediated Ba activation was induced with a Dp allergenic extract. With Dc 1 μg/ml, mean HBDT in Dc-susceptible individuals was 33.62 ± 18.35% and 8.49 ± 4.79% in controls (P = 0.0001). Mean BAT was 2.04 ± 1.68% and 1.93 ± 1.40% in controls (P = 0.8). Ba preincubation with Dc did not affect fMLP-induced CD63 expression, neither in Dc-sensitive individuals (P = 0.8) (n = 4) nor in subjects without Dc hypersensitivity (P = 0.25) (n = 4). Ba from the two patients sensitive both to Dc and Dp responded to Dp but not to Dc by BAT: Dc, 1.99 ± 0.78%; Dp: 60.87 ± 9.28%; but showed degranulation by HBDT: Dc, 30.53 ± 1.02%, Dp: 48.78 ± 22.17%. Dc induces Ba degranulation in sensitive patients in a way that does not induce CD63 expression and is different from IgE-mediated and fMLP-mediated degranulation. Our results suggest that CD63 expression is not a reliable diagnostic method for diclofenac allergy. © 2006 The Author(s).Fil: Malbrán, Alejandro. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Hospital Británico de Buenos Aires; ArgentinaFil: Yeyati, E.. Hospital Británico de Buenos Aires; ArgentinaFil: Rey, G. L.. Hospital Británico de Buenos Aires; ArgentinaFil: Galassi, Nora Virginia. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Academia Nacional de Medicina de Buenos Aires; ArgentinaWiley Blackwell Publishing, Inc2007-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/56438Malbrán, Alejandro; Yeyati, E.; Rey, G. L.; Galassi, Nora Virginia; Diclofenac induces basophil degranulation without increasing CD63 expression in sensitive patients; Wiley Blackwell Publishing, Inc; Clinical and Experimental Immunology; 147; 1; 1-2007; 99-1050009-9104CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.1111/j.1365-2249.2006.03265.xinfo:eu-repo/semantics/altIdentifier/url/https://onlinelibrary.wiley.com/doi/abs/10.1111/j.1365-2249.2006.03265.xinfo:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-03T10:09:56Zoai:ri.conicet.gov.ar:11336/56438instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-03 10:09:56.626CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Diclofenac induces basophil degranulation without increasing CD63 expression in sensitive patients
title Diclofenac induces basophil degranulation without increasing CD63 expression in sensitive patients
spellingShingle Diclofenac induces basophil degranulation without increasing CD63 expression in sensitive patients
Malbrán, Alejandro
Allergy
Basophil
Cd63
Exocytosis
Nsaids
title_short Diclofenac induces basophil degranulation without increasing CD63 expression in sensitive patients
title_full Diclofenac induces basophil degranulation without increasing CD63 expression in sensitive patients
title_fullStr Diclofenac induces basophil degranulation without increasing CD63 expression in sensitive patients
title_full_unstemmed Diclofenac induces basophil degranulation without increasing CD63 expression in sensitive patients
title_sort Diclofenac induces basophil degranulation without increasing CD63 expression in sensitive patients
dc.creator.none.fl_str_mv Malbrán, Alejandro
Yeyati, E.
Rey, G. L.
Galassi, Nora Virginia
author Malbrán, Alejandro
author_facet Malbrán, Alejandro
Yeyati, E.
Rey, G. L.
Galassi, Nora Virginia
author_role author
author2 Yeyati, E.
Rey, G. L.
Galassi, Nora Virginia
author2_role author
author
author
dc.subject.none.fl_str_mv Allergy
Basophil
Cd63
Exocytosis
Nsaids
topic Allergy
Basophil
Cd63
Exocytosis
Nsaids
purl_subject.fl_str_mv https://purl.org/becyt/ford/3.2
https://purl.org/becyt/ford/3
dc.description.none.fl_txt_mv Diclofenac (Dc) induces an IgE-independent basophil (Ba) degranulation in susceptible individuals. CD63 Ba expression is utilized as an in vitro test for diagnosis of drug hypersensitivity. We tested the ability of Dc to induce CD63 Ba expression by flow cytometry (BAT) and Ba degranulation using light microscopy (HBDT) in patients sensitive to Dc. We studied 14 patients with diclofenac hypersensitivity, also two patients sensitive to Dermatophagoides pteronyssinus (Dp), and 12 normal controls. HBDT was performed by mononuclear cells toluidine blue staining. BAT determined CD63 expression in antiCD63/anti-IgE/anti-CD45-labelled whole blood. In each case, the percentage of activated Ba post-stimulation with 1 and 10 μg/ml Dc was determined. Positive controls included N-formyl-methionyl-leucyl-phenylalanine (fMLP) peptide-induced activation. IgE-mediated Ba activation was induced with a Dp allergenic extract. With Dc 1 μg/ml, mean HBDT in Dc-susceptible individuals was 33.62 ± 18.35% and 8.49 ± 4.79% in controls (P = 0.0001). Mean BAT was 2.04 ± 1.68% and 1.93 ± 1.40% in controls (P = 0.8). Ba preincubation with Dc did not affect fMLP-induced CD63 expression, neither in Dc-sensitive individuals (P = 0.8) (n = 4) nor in subjects without Dc hypersensitivity (P = 0.25) (n = 4). Ba from the two patients sensitive both to Dc and Dp responded to Dp but not to Dc by BAT: Dc, 1.99 ± 0.78%; Dp: 60.87 ± 9.28%; but showed degranulation by HBDT: Dc, 30.53 ± 1.02%, Dp: 48.78 ± 22.17%. Dc induces Ba degranulation in sensitive patients in a way that does not induce CD63 expression and is different from IgE-mediated and fMLP-mediated degranulation. Our results suggest that CD63 expression is not a reliable diagnostic method for diclofenac allergy. © 2006 The Author(s).
Fil: Malbrán, Alejandro. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Hospital Británico de Buenos Aires; Argentina
Fil: Yeyati, E.. Hospital Británico de Buenos Aires; Argentina
Fil: Rey, G. L.. Hospital Británico de Buenos Aires; Argentina
Fil: Galassi, Nora Virginia. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Academia Nacional de Medicina de Buenos Aires; Argentina
description Diclofenac (Dc) induces an IgE-independent basophil (Ba) degranulation in susceptible individuals. CD63 Ba expression is utilized as an in vitro test for diagnosis of drug hypersensitivity. We tested the ability of Dc to induce CD63 Ba expression by flow cytometry (BAT) and Ba degranulation using light microscopy (HBDT) in patients sensitive to Dc. We studied 14 patients with diclofenac hypersensitivity, also two patients sensitive to Dermatophagoides pteronyssinus (Dp), and 12 normal controls. HBDT was performed by mononuclear cells toluidine blue staining. BAT determined CD63 expression in antiCD63/anti-IgE/anti-CD45-labelled whole blood. In each case, the percentage of activated Ba post-stimulation with 1 and 10 μg/ml Dc was determined. Positive controls included N-formyl-methionyl-leucyl-phenylalanine (fMLP) peptide-induced activation. IgE-mediated Ba activation was induced with a Dp allergenic extract. With Dc 1 μg/ml, mean HBDT in Dc-susceptible individuals was 33.62 ± 18.35% and 8.49 ± 4.79% in controls (P = 0.0001). Mean BAT was 2.04 ± 1.68% and 1.93 ± 1.40% in controls (P = 0.8). Ba preincubation with Dc did not affect fMLP-induced CD63 expression, neither in Dc-sensitive individuals (P = 0.8) (n = 4) nor in subjects without Dc hypersensitivity (P = 0.25) (n = 4). Ba from the two patients sensitive both to Dc and Dp responded to Dp but not to Dc by BAT: Dc, 1.99 ± 0.78%; Dp: 60.87 ± 9.28%; but showed degranulation by HBDT: Dc, 30.53 ± 1.02%, Dp: 48.78 ± 22.17%. Dc induces Ba degranulation in sensitive patients in a way that does not induce CD63 expression and is different from IgE-mediated and fMLP-mediated degranulation. Our results suggest that CD63 expression is not a reliable diagnostic method for diclofenac allergy. © 2006 The Author(s).
publishDate 2007
dc.date.none.fl_str_mv 2007-01
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/56438
Malbrán, Alejandro; Yeyati, E.; Rey, G. L.; Galassi, Nora Virginia; Diclofenac induces basophil degranulation without increasing CD63 expression in sensitive patients; Wiley Blackwell Publishing, Inc; Clinical and Experimental Immunology; 147; 1; 1-2007; 99-105
0009-9104
CONICET Digital
CONICET
url http://hdl.handle.net/11336/56438
identifier_str_mv Malbrán, Alejandro; Yeyati, E.; Rey, G. L.; Galassi, Nora Virginia; Diclofenac induces basophil degranulation without increasing CD63 expression in sensitive patients; Wiley Blackwell Publishing, Inc; Clinical and Experimental Immunology; 147; 1; 1-2007; 99-105
0009-9104
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.1111/j.1365-2249.2006.03265.x
info:eu-repo/semantics/altIdentifier/url/https://onlinelibrary.wiley.com/doi/abs/10.1111/j.1365-2249.2006.03265.x
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Wiley Blackwell Publishing, Inc
publisher.none.fl_str_mv Wiley Blackwell Publishing, Inc
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.13397

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