Enhanced chitin gel with magnetic nanofiller for lysozyme purification

Autores
Tovar Jimenez, Gabriel Ibrahin; Fernández de Luis, Roberto; Arriortua, María Isabel; Wolman, Federico Javier; Copello, Guillermo Javier
Año de publicación
2020
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
In this study, we have investigated the impact of superparamagnetic magnetite nanoparticle (Fe3O4) inclusion on the chitin polysaccharide structure, together with its surface chemistry influence on the adsorption of lysozyme (LYZ). Magnetic nanoparticles (MNPs) as fillers not only endow the chitin host structure with their physic and chemical properties but also is a straightforward tool to modify or template its porous structure. Indeed, scanning electron microscopy and Brunauer–Emmett–Teller surface area measurements confirm the template effect of the MNPs on chitin. Their incorporation reduced the thickness of the pore wall and increased the surface area from chitin (34.5 m2/g) to the chitin@Fe3O4 composites (210.8 m2/g). MNPs provide the composite system an intrinsic magnetic moment that enables the magnetic recovery of the adsorbent after LYZ uptake. To characterize the magnetic composite's interaction with LYZ, the effect of pH on the absorptive capacities and kinetic parameters was examined. The results indicated that the nanocomposite presents an adsorption capacity of 488 mg/g of lysozyme at pH 9, being able to recover LYZ without diluting or pretreating the hen egg-white, leading to a 75% global yield and a purity degree >99% in only one chromatographic step.
Fil: Tovar Jimenez, Gabriel Ibrahin. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Química y Metabolismo del Fármaco. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Instituto de Química y Metabolismo del Fármaco; Argentina
Fil: Fernández de Luis, Roberto. No especifíca;
Fil: Arriortua, María Isabel. Universidad del País Vasco; España
Fil: Wolman, Federico Javier. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Nanobiotecnología. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Instituto de Nanobiotecnología; Argentina
Fil: Copello, Guillermo Javier. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Química y Metabolismo del Fármaco. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Instituto de Química y Metabolismo del Fármaco; Argentina
Materia
CHITIN
LYSOZYME
MAGNETITE
NANOFILLER
PROTEIN PURIFICATION
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/139878
Acceder
id CONICETDig_5c5629fb6c7fa4ee227ae84c392b370a
oai_identifier_str oai:ri.conicet.gov.ar:11336/139878
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Enhanced chitin gel with magnetic nanofiller for lysozyme purificationTovar Jimenez, Gabriel IbrahinFernández de Luis, RobertoArriortua, María IsabelWolman, Federico JavierCopello, Guillermo JavierCHITINLYSOZYMEMAGNETITENANOFILLERPROTEIN PURIFICATIONhttps://purl.org/becyt/ford/2.10https://purl.org/becyt/ford/2In this study, we have investigated the impact of superparamagnetic magnetite nanoparticle (Fe3O4) inclusion on the chitin polysaccharide structure, together with its surface chemistry influence on the adsorption of lysozyme (LYZ). Magnetic nanoparticles (MNPs) as fillers not only endow the chitin host structure with their physic and chemical properties but also is a straightforward tool to modify or template its porous structure. Indeed, scanning electron microscopy and Brunauer–Emmett–Teller surface area measurements confirm the template effect of the MNPs on chitin. Their incorporation reduced the thickness of the pore wall and increased the surface area from chitin (34.5 m2/g) to the chitin@Fe3O4 composites (210.8 m2/g). MNPs provide the composite system an intrinsic magnetic moment that enables the magnetic recovery of the adsorbent after LYZ uptake. To characterize the magnetic composite's interaction with LYZ, the effect of pH on the absorptive capacities and kinetic parameters was examined. The results indicated that the nanocomposite presents an adsorption capacity of 488 mg/g of lysozyme at pH 9, being able to recover LYZ without diluting or pretreating the hen egg-white, leading to a 75% global yield and a purity degree >99% in only one chromatographic step.Fil: Tovar Jimenez, Gabriel Ibrahin. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Química y Metabolismo del Fármaco. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Instituto de Química y Metabolismo del Fármaco; ArgentinaFil: Fernández de Luis, Roberto. No especifíca;Fil: Arriortua, María Isabel. Universidad del País Vasco; EspañaFil: Wolman, Federico Javier. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Nanobiotecnología. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Instituto de Nanobiotecnología; ArgentinaFil: Copello, Guillermo Javier. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Química y Metabolismo del Fármaco. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Instituto de Química y Metabolismo del Fármaco; ArgentinaKorean Soc Industrial Engineering Chemistry2020-08info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/139878Tovar Jimenez, Gabriel Ibrahin; Fernández de Luis, Roberto; Arriortua, María Isabel; Wolman, Federico Javier; Copello, Guillermo Javier; Enhanced chitin gel with magnetic nanofiller for lysozyme purification; Korean Soc Industrial Engineering Chemistry; Journal Of Industrial And Engineering Chemistry; 88; 8-2020; 90-981226-086XCONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://linkinghub.elsevier.com/retrieve/pii/S1226086X2030143Xinfo:eu-repo/semantics/altIdentifier/doi/10.1016/j.jiec.2020.03.026info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-nd/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-10-22T11:18:14Zoai:ri.conicet.gov.ar:11336/139878instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-10-22 11:18:14.73CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Enhanced chitin gel with magnetic nanofiller for lysozyme purification
title Enhanced chitin gel with magnetic nanofiller for lysozyme purification
spellingShingle Enhanced chitin gel with magnetic nanofiller for lysozyme purification
Tovar Jimenez, Gabriel Ibrahin
CHITIN
LYSOZYME
MAGNETITE
NANOFILLER
PROTEIN PURIFICATION
title_short Enhanced chitin gel with magnetic nanofiller for lysozyme purification
title_full Enhanced chitin gel with magnetic nanofiller for lysozyme purification
title_fullStr Enhanced chitin gel with magnetic nanofiller for lysozyme purification
title_full_unstemmed Enhanced chitin gel with magnetic nanofiller for lysozyme purification
title_sort Enhanced chitin gel with magnetic nanofiller for lysozyme purification
dc.creator.none.fl_str_mv Tovar Jimenez, Gabriel Ibrahin
Fernández de Luis, Roberto
Arriortua, María Isabel
Wolman, Federico Javier
Copello, Guillermo Javier
author Tovar Jimenez, Gabriel Ibrahin
author_facet Tovar Jimenez, Gabriel Ibrahin
Fernández de Luis, Roberto
Arriortua, María Isabel
Wolman, Federico Javier
Copello, Guillermo Javier
author_role author
author2 Fernández de Luis, Roberto
Arriortua, María Isabel
Wolman, Federico Javier
Copello, Guillermo Javier
author2_role author
author
author
author
dc.subject.none.fl_str_mv CHITIN
LYSOZYME
MAGNETITE
NANOFILLER
PROTEIN PURIFICATION
topic CHITIN
LYSOZYME
MAGNETITE
NANOFILLER
PROTEIN PURIFICATION
purl_subject.fl_str_mv https://purl.org/becyt/ford/2.10
https://purl.org/becyt/ford/2
dc.description.none.fl_txt_mv In this study, we have investigated the impact of superparamagnetic magnetite nanoparticle (Fe3O4) inclusion on the chitin polysaccharide structure, together with its surface chemistry influence on the adsorption of lysozyme (LYZ). Magnetic nanoparticles (MNPs) as fillers not only endow the chitin host structure with their physic and chemical properties but also is a straightforward tool to modify or template its porous structure. Indeed, scanning electron microscopy and Brunauer–Emmett–Teller surface area measurements confirm the template effect of the MNPs on chitin. Their incorporation reduced the thickness of the pore wall and increased the surface area from chitin (34.5 m2/g) to the chitin@Fe3O4 composites (210.8 m2/g). MNPs provide the composite system an intrinsic magnetic moment that enables the magnetic recovery of the adsorbent after LYZ uptake. To characterize the magnetic composite's interaction with LYZ, the effect of pH on the absorptive capacities and kinetic parameters was examined. The results indicated that the nanocomposite presents an adsorption capacity of 488 mg/g of lysozyme at pH 9, being able to recover LYZ without diluting or pretreating the hen egg-white, leading to a 75% global yield and a purity degree >99% in only one chromatographic step.
Fil: Tovar Jimenez, Gabriel Ibrahin. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Química y Metabolismo del Fármaco. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Instituto de Química y Metabolismo del Fármaco; Argentina
Fil: Fernández de Luis, Roberto. No especifíca;
Fil: Arriortua, María Isabel. Universidad del País Vasco; España
Fil: Wolman, Federico Javier. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Nanobiotecnología. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Instituto de Nanobiotecnología; Argentina
Fil: Copello, Guillermo Javier. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Química y Metabolismo del Fármaco. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Instituto de Química y Metabolismo del Fármaco; Argentina
description In this study, we have investigated the impact of superparamagnetic magnetite nanoparticle (Fe3O4) inclusion on the chitin polysaccharide structure, together with its surface chemistry influence on the adsorption of lysozyme (LYZ). Magnetic nanoparticles (MNPs) as fillers not only endow the chitin host structure with their physic and chemical properties but also is a straightforward tool to modify or template its porous structure. Indeed, scanning electron microscopy and Brunauer–Emmett–Teller surface area measurements confirm the template effect of the MNPs on chitin. Their incorporation reduced the thickness of the pore wall and increased the surface area from chitin (34.5 m2/g) to the chitin@Fe3O4 composites (210.8 m2/g). MNPs provide the composite system an intrinsic magnetic moment that enables the magnetic recovery of the adsorbent after LYZ uptake. To characterize the magnetic composite's interaction with LYZ, the effect of pH on the absorptive capacities and kinetic parameters was examined. The results indicated that the nanocomposite presents an adsorption capacity of 488 mg/g of lysozyme at pH 9, being able to recover LYZ without diluting or pretreating the hen egg-white, leading to a 75% global yield and a purity degree >99% in only one chromatographic step.
publishDate 2020
dc.date.none.fl_str_mv 2020-08
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/139878
Tovar Jimenez, Gabriel Ibrahin; Fernández de Luis, Roberto; Arriortua, María Isabel; Wolman, Federico Javier; Copello, Guillermo Javier; Enhanced chitin gel with magnetic nanofiller for lysozyme purification; Korean Soc Industrial Engineering Chemistry; Journal Of Industrial And Engineering Chemistry; 88; 8-2020; 90-98
1226-086X
CONICET Digital
CONICET
url http://hdl.handle.net/11336/139878
identifier_str_mv Tovar Jimenez, Gabriel Ibrahin; Fernández de Luis, Roberto; Arriortua, María Isabel; Wolman, Federico Javier; Copello, Guillermo Javier; Enhanced chitin gel with magnetic nanofiller for lysozyme purification; Korean Soc Industrial Engineering Chemistry; Journal Of Industrial And Engineering Chemistry; 88; 8-2020; 90-98
1226-086X
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://linkinghub.elsevier.com/retrieve/pii/S1226086X2030143X
info:eu-repo/semantics/altIdentifier/doi/10.1016/j.jiec.2020.03.026
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Korean Soc Industrial Engineering Chemistry
publisher.none.fl_str_mv Korean Soc Industrial Engineering Chemistry
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 12.982451

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