Deleterious effects induced by oxidative stress in liver nuclei from rats receiving an alcohol-containing liquid diet

Autores
Diaz Gomez, Maria Isabel; Fanelli, Silvia Laura; Delgado, Aurora Maria; Bietto, Florencia Matilde; Castro, Jose Alberto; Castro, Gerardo Daniel
Año de publicación
2008
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Highly purified rat-liver nuclei were previously shown to have nuclear ethanol (EtOH) metabolizing system able to bioactivate alcohol to acetaldehyde and 1-hydroxyethyl radicals. These reactive metabolites were able to covalently bind to nuclear proteins and lipids potentially being able to provoke oxidative stress of nuclear components. In this study, the above-mentioned possibility was explored. Sprague Dawley male rats (125–150 g) were fed a standard Lieber and De Carli liquid diet for 28 days. Controls were pair-fed with a diet, in which EtOH was isocalorically replaced with carbohydrate. The presence of a chlorzoxazone hydroxylase activity inducible by the repetitive EtOH drinking further suggested the presence of CYP2E1 in the highly purified nuclei. Nuclei from EtOH-drinking rats evidenced significantly increased susceptibility to a t-butyl hydroperoxide challenge as detected by chemiluminescence emission, increased formation of protein carbonyls, and decreased content of protein sulfhydryls. In contrast, no significant changes in the nuclear lipid hydroperoxides formation or even decreases in the 8-oxo-7,8-dihydro-2-deoxyguanosine were observed. No significant differences were observed in different parameters of the alkaline Comet assay. In immunohistochemical studies performed, no expression of p53 was observed in the livers of the animals under the experimental conditions tested. Since nuclear proteins and lipids are known to play a role in cell growth, differentiation, repair and signaling, their alterations by either oxidative stress, or by covalent binding might be of relevance to liver tumor promotion.
Fil: Diaz Gomez, Maria Isabel. Consejo Nacional de Investigaciones Científicas y Técnicas. Gp.citefa - Centro de Invest.toxicológicas (i); Argentina
Fil: Fanelli, Silvia Laura. Consejo Nacional de Investigaciones Científicas y Técnicas. Gp.citefa - Centro de Invest.toxicológicas (i); Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Limnología "Dr. Raúl A. Ringuelet". Universidad Nacional de La Plata. Facultad de Ciencias Naturales y Museo. Instituto de Limnología; Argentina
Fil: Delgado, Aurora Maria. Consejo Nacional de Investigaciones Científicas y Técnicas. Gp.citefa - Centro de Invest.toxicológicas (i); Argentina
Fil: Bietto, Florencia Matilde. Consejo Nacional de Investigaciones Científicas y Técnicas. Gp.citefa - Centro de Invest.toxicológicas (i); Argentina
Fil: Castro, Jose Alberto. Consejo Nacional de Investigaciones Científicas y Técnicas. Gp.citefa - Centro de Invest.toxicológicas (i); Argentina
Fil: Castro, Gerardo Daniel. Consejo Nacional de Investigaciones Científicas y Técnicas. Gp.citefa - Centro de Invest.toxicológicas (i); Argentina
Materia
Alcohol
Cyp2e1
Ethanol
Nuclei
Microsomes
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/25990
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/25990
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Deleterious effects induced by oxidative stress in liver nuclei from rats receiving an alcohol-containing liquid dietDiaz Gomez, Maria IsabelFanelli, Silvia LauraDelgado, Aurora MariaBietto, Florencia MatildeCastro, Jose AlbertoCastro, Gerardo DanielAlcoholCyp2e1EthanolNucleiMicrosomeshttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1Highly purified rat-liver nuclei were previously shown to have nuclear ethanol (EtOH) metabolizing system able to bioactivate alcohol to acetaldehyde and 1-hydroxyethyl radicals. These reactive metabolites were able to covalently bind to nuclear proteins and lipids potentially being able to provoke oxidative stress of nuclear components. In this study, the above-mentioned possibility was explored. Sprague Dawley male rats (125–150 g) were fed a standard Lieber and De Carli liquid diet for 28 days. Controls were pair-fed with a diet, in which EtOH was isocalorically replaced with carbohydrate. The presence of a chlorzoxazone hydroxylase activity inducible by the repetitive EtOH drinking further suggested the presence of CYP2E1 in the highly purified nuclei. Nuclei from EtOH-drinking rats evidenced significantly increased susceptibility to a t-butyl hydroperoxide challenge as detected by chemiluminescence emission, increased formation of protein carbonyls, and decreased content of protein sulfhydryls. In contrast, no significant changes in the nuclear lipid hydroperoxides formation or even decreases in the 8-oxo-7,8-dihydro-2-deoxyguanosine were observed. No significant differences were observed in different parameters of the alkaline Comet assay. In immunohistochemical studies performed, no expression of p53 was observed in the livers of the animals under the experimental conditions tested. Since nuclear proteins and lipids are known to play a role in cell growth, differentiation, repair and signaling, their alterations by either oxidative stress, or by covalent binding might be of relevance to liver tumor promotion.Fil: Diaz Gomez, Maria Isabel. Consejo Nacional de Investigaciones Científicas y Técnicas. Gp.citefa - Centro de Invest.toxicológicas (i); ArgentinaFil: Fanelli, Silvia Laura. Consejo Nacional de Investigaciones Científicas y Técnicas. Gp.citefa - Centro de Invest.toxicológicas (i); Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Limnología "Dr. Raúl A. Ringuelet". Universidad Nacional de La Plata. Facultad de Ciencias Naturales y Museo. Instituto de Limnología; ArgentinaFil: Delgado, Aurora Maria. Consejo Nacional de Investigaciones Científicas y Técnicas. Gp.citefa - Centro de Invest.toxicológicas (i); ArgentinaFil: Bietto, Florencia Matilde. Consejo Nacional de Investigaciones Científicas y Técnicas. Gp.citefa - Centro de Invest.toxicológicas (i); ArgentinaFil: Castro, Jose Alberto. Consejo Nacional de Investigaciones Científicas y Técnicas. Gp.citefa - Centro de Invest.toxicológicas (i); ArgentinaFil: Castro, Gerardo Daniel. Consejo Nacional de Investigaciones Científicas y Técnicas. Gp.citefa - Centro de Invest.toxicológicas (i); ArgentinaSage Publications Ltd2008-11info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/25990Diaz Gomez, Maria Isabel; Fanelli, Silvia Laura; Delgado, Aurora Maria; Bietto, Florencia Matilde; Castro, Jose Alberto; et al.; Deleterious effects induced by oxidative stress in liver nuclei from rats receiving an alcohol-containing liquid diet; Sage Publications Ltd; Toxicology And Industrial Health; 24; 10; 11-2008; 625-6340748-2337CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/http://journals.sagepub.com/doi/10.1177/0748233708101207info:eu-repo/semantics/altIdentifier/doi/10.1177/0748233708101207info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-03T09:45:08Zoai:ri.conicet.gov.ar:11336/25990instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-03 09:45:08.471CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Deleterious effects induced by oxidative stress in liver nuclei from rats receiving an alcohol-containing liquid diet
title Deleterious effects induced by oxidative stress in liver nuclei from rats receiving an alcohol-containing liquid diet
spellingShingle Deleterious effects induced by oxidative stress in liver nuclei from rats receiving an alcohol-containing liquid diet
Diaz Gomez, Maria Isabel
Alcohol
Cyp2e1
Ethanol
Nuclei
Microsomes
title_short Deleterious effects induced by oxidative stress in liver nuclei from rats receiving an alcohol-containing liquid diet
title_full Deleterious effects induced by oxidative stress in liver nuclei from rats receiving an alcohol-containing liquid diet
title_fullStr Deleterious effects induced by oxidative stress in liver nuclei from rats receiving an alcohol-containing liquid diet
title_full_unstemmed Deleterious effects induced by oxidative stress in liver nuclei from rats receiving an alcohol-containing liquid diet
title_sort Deleterious effects induced by oxidative stress in liver nuclei from rats receiving an alcohol-containing liquid diet
dc.creator.none.fl_str_mv Diaz Gomez, Maria Isabel
Fanelli, Silvia Laura
Delgado, Aurora Maria
Bietto, Florencia Matilde
Castro, Jose Alberto
Castro, Gerardo Daniel
author Diaz Gomez, Maria Isabel
author_facet Diaz Gomez, Maria Isabel
Fanelli, Silvia Laura
Delgado, Aurora Maria
Bietto, Florencia Matilde
Castro, Jose Alberto
Castro, Gerardo Daniel
author_role author
author2 Fanelli, Silvia Laura
Delgado, Aurora Maria
Bietto, Florencia Matilde
Castro, Jose Alberto
Castro, Gerardo Daniel
author2_role author
author
author
author
author
dc.subject.none.fl_str_mv Alcohol
Cyp2e1
Ethanol
Nuclei
Microsomes
topic Alcohol
Cyp2e1
Ethanol
Nuclei
Microsomes
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv Highly purified rat-liver nuclei were previously shown to have nuclear ethanol (EtOH) metabolizing system able to bioactivate alcohol to acetaldehyde and 1-hydroxyethyl radicals. These reactive metabolites were able to covalently bind to nuclear proteins and lipids potentially being able to provoke oxidative stress of nuclear components. In this study, the above-mentioned possibility was explored. Sprague Dawley male rats (125–150 g) were fed a standard Lieber and De Carli liquid diet for 28 days. Controls were pair-fed with a diet, in which EtOH was isocalorically replaced with carbohydrate. The presence of a chlorzoxazone hydroxylase activity inducible by the repetitive EtOH drinking further suggested the presence of CYP2E1 in the highly purified nuclei. Nuclei from EtOH-drinking rats evidenced significantly increased susceptibility to a t-butyl hydroperoxide challenge as detected by chemiluminescence emission, increased formation of protein carbonyls, and decreased content of protein sulfhydryls. In contrast, no significant changes in the nuclear lipid hydroperoxides formation or even decreases in the 8-oxo-7,8-dihydro-2-deoxyguanosine were observed. No significant differences were observed in different parameters of the alkaline Comet assay. In immunohistochemical studies performed, no expression of p53 was observed in the livers of the animals under the experimental conditions tested. Since nuclear proteins and lipids are known to play a role in cell growth, differentiation, repair and signaling, their alterations by either oxidative stress, or by covalent binding might be of relevance to liver tumor promotion.
Fil: Diaz Gomez, Maria Isabel. Consejo Nacional de Investigaciones Científicas y Técnicas. Gp.citefa - Centro de Invest.toxicológicas (i); Argentina
Fil: Fanelli, Silvia Laura. Consejo Nacional de Investigaciones Científicas y Técnicas. Gp.citefa - Centro de Invest.toxicológicas (i); Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Limnología "Dr. Raúl A. Ringuelet". Universidad Nacional de La Plata. Facultad de Ciencias Naturales y Museo. Instituto de Limnología; Argentina
Fil: Delgado, Aurora Maria. Consejo Nacional de Investigaciones Científicas y Técnicas. Gp.citefa - Centro de Invest.toxicológicas (i); Argentina
Fil: Bietto, Florencia Matilde. Consejo Nacional de Investigaciones Científicas y Técnicas. Gp.citefa - Centro de Invest.toxicológicas (i); Argentina
Fil: Castro, Jose Alberto. Consejo Nacional de Investigaciones Científicas y Técnicas. Gp.citefa - Centro de Invest.toxicológicas (i); Argentina
Fil: Castro, Gerardo Daniel. Consejo Nacional de Investigaciones Científicas y Técnicas. Gp.citefa - Centro de Invest.toxicológicas (i); Argentina
description Highly purified rat-liver nuclei were previously shown to have nuclear ethanol (EtOH) metabolizing system able to bioactivate alcohol to acetaldehyde and 1-hydroxyethyl radicals. These reactive metabolites were able to covalently bind to nuclear proteins and lipids potentially being able to provoke oxidative stress of nuclear components. In this study, the above-mentioned possibility was explored. Sprague Dawley male rats (125–150 g) were fed a standard Lieber and De Carli liquid diet for 28 days. Controls were pair-fed with a diet, in which EtOH was isocalorically replaced with carbohydrate. The presence of a chlorzoxazone hydroxylase activity inducible by the repetitive EtOH drinking further suggested the presence of CYP2E1 in the highly purified nuclei. Nuclei from EtOH-drinking rats evidenced significantly increased susceptibility to a t-butyl hydroperoxide challenge as detected by chemiluminescence emission, increased formation of protein carbonyls, and decreased content of protein sulfhydryls. In contrast, no significant changes in the nuclear lipid hydroperoxides formation or even decreases in the 8-oxo-7,8-dihydro-2-deoxyguanosine were observed. No significant differences were observed in different parameters of the alkaline Comet assay. In immunohistochemical studies performed, no expression of p53 was observed in the livers of the animals under the experimental conditions tested. Since nuclear proteins and lipids are known to play a role in cell growth, differentiation, repair and signaling, their alterations by either oxidative stress, or by covalent binding might be of relevance to liver tumor promotion.
publishDate 2008
dc.date.none.fl_str_mv 2008-11
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/25990
Diaz Gomez, Maria Isabel; Fanelli, Silvia Laura; Delgado, Aurora Maria; Bietto, Florencia Matilde; Castro, Jose Alberto; et al.; Deleterious effects induced by oxidative stress in liver nuclei from rats receiving an alcohol-containing liquid diet; Sage Publications Ltd; Toxicology And Industrial Health; 24; 10; 11-2008; 625-634
0748-2337
CONICET Digital
CONICET
url http://hdl.handle.net/11336/25990
identifier_str_mv Diaz Gomez, Maria Isabel; Fanelli, Silvia Laura; Delgado, Aurora Maria; Bietto, Florencia Matilde; Castro, Jose Alberto; et al.; Deleterious effects induced by oxidative stress in liver nuclei from rats receiving an alcohol-containing liquid diet; Sage Publications Ltd; Toxicology And Industrial Health; 24; 10; 11-2008; 625-634
0748-2337
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/http://journals.sagepub.com/doi/10.1177/0748233708101207
info:eu-repo/semantics/altIdentifier/doi/10.1177/0748233708101207
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Sage Publications Ltd
publisher.none.fl_str_mv Sage Publications Ltd
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.13397

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