Continuous-flow/stopped-flow system incorporating a rotating bioreactor based on a double redox catalytic cycle and electron transfer mediated by osmium complexes. Application in t...

Autores
Salinas, Eloy; Morcillo, Pablo; Sanz Ferramola, Maria Isabel; Raba, Julio
Año de publicación
2002
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
The high sensitivity that can be attained by enzymatic amplification via substrate cycling and mediated by the redox polymer [Os(bpy)2ClPyCH2NHpoly( allylamine) (Os-PAA), has been verified by on-line interfacing of a rotating bioreactor and continuous-flow=stopped-flow=continuous-flow processing system. The determination of glucose was possible with a limit of detection of 20 fmol L1 in the processing of as many as 30 samples per hour. Determination at such low levels is of interest in several situations encountered in fermentation biotechnology and clinical chemistry, and for the determination in culture broths; it illustrates the capabilities of the proposed approach. Glucose oxidase and Os-PAA were covalently immobilized on a glassy carbon electrode surface (upper cell body), Glucose dehydrogenase [EC 1.1.1.47] was immobilized on a disk that can be rotated. Substrate cycling was realized via NADH=NADþ that, in conjunction with glucose dehydrogenase, regenerates glucose, the substrate in the glucose oxidase-catalyzed reaction.
Fil: Salinas, Eloy. Universidad Nacional de San Luis; Argentina
Fil: Morcillo, Pablo. Universidad Nacional de San Luis; Argentina
Fil: Sanz Ferramola, Maria Isabel. Universidad Nacional de San Luis; Argentina
Fil: Raba, Julio. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - San Luis. Instituto de Química de San Luis. Universidad Nacional de San Luis. Facultad de Química, Bioquímica y Farmacia. Instituto de Química de San Luis; Argentina
Materia
ROTATING BIOREACTOR
CATALYTIC CYCLE
ELECTRON TRANSFER
OSMIUM COMPLEXES
LEVELS OF GLUCOSE
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/102178
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/102178
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Continuous-flow/stopped-flow system incorporating a rotating bioreactor based on a double redox catalytic cycle and electron transfer mediated by osmium complexes. Application in the determination of extremely low levels of glucoseSalinas, EloyMorcillo, PabloSanz Ferramola, Maria IsabelRaba, JulioROTATING BIOREACTORCATALYTIC CYCLEELECTRON TRANSFEROSMIUM COMPLEXESLEVELS OF GLUCOSEhttps://purl.org/becyt/ford/1.4https://purl.org/becyt/ford/1The high sensitivity that can be attained by enzymatic amplification via substrate cycling and mediated by the redox polymer [Os(bpy)2ClPyCH2NHpoly( allylamine) (Os-PAA), has been verified by on-line interfacing of a rotating bioreactor and continuous-flow=stopped-flow=continuous-flow processing system. The determination of glucose was possible with a limit of detection of 20 fmol L1 in the processing of as many as 30 samples per hour. Determination at such low levels is of interest in several situations encountered in fermentation biotechnology and clinical chemistry, and for the determination in culture broths; it illustrates the capabilities of the proposed approach. Glucose oxidase and Os-PAA were covalently immobilized on a glassy carbon electrode surface (upper cell body), Glucose dehydrogenase [EC 1.1.1.47] was immobilized on a disk that can be rotated. Substrate cycling was realized via NADH=NADþ that, in conjunction with glucose dehydrogenase, regenerates glucose, the substrate in the glucose oxidase-catalyzed reaction.Fil: Salinas, Eloy. Universidad Nacional de San Luis; ArgentinaFil: Morcillo, Pablo. Universidad Nacional de San Luis; ArgentinaFil: Sanz Ferramola, Maria Isabel. Universidad Nacional de San Luis; ArgentinaFil: Raba, Julio. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - San Luis. Instituto de Química de San Luis. Universidad Nacional de San Luis. Facultad de Química, Bioquímica y Farmacia. Instituto de Química de San Luis; ArgentinaTaylor & Francis2002-02info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/102178Salinas, Eloy; Morcillo, Pablo; Sanz Ferramola, Maria Isabel; Raba, Julio; Continuous-flow/stopped-flow system incorporating a rotating bioreactor based on a double redox catalytic cycle and electron transfer mediated by osmium complexes. Application in the determination of extremely low levels of glucose; Taylor & Francis; Instrumentation Science And Technology; 30; 3; 2-2002; 281-2931073-91491525-6030CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://www.tandfonline.com/doi/abs/10.1081/CI-120013507info:eu-repo/semantics/altIdentifier/doi/10.1081/CI-120013507info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T10:07:03Zoai:ri.conicet.gov.ar:11336/102178instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 10:07:03.606CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Continuous-flow/stopped-flow system incorporating a rotating bioreactor based on a double redox catalytic cycle and electron transfer mediated by osmium complexes. Application in the determination of extremely low levels of glucose
title Continuous-flow/stopped-flow system incorporating a rotating bioreactor based on a double redox catalytic cycle and electron transfer mediated by osmium complexes. Application in the determination of extremely low levels of glucose
spellingShingle Continuous-flow/stopped-flow system incorporating a rotating bioreactor based on a double redox catalytic cycle and electron transfer mediated by osmium complexes. Application in the determination of extremely low levels of glucose
Salinas, Eloy
ROTATING BIOREACTOR
CATALYTIC CYCLE
ELECTRON TRANSFER
OSMIUM COMPLEXES
LEVELS OF GLUCOSE
title_short Continuous-flow/stopped-flow system incorporating a rotating bioreactor based on a double redox catalytic cycle and electron transfer mediated by osmium complexes. Application in the determination of extremely low levels of glucose
title_full Continuous-flow/stopped-flow system incorporating a rotating bioreactor based on a double redox catalytic cycle and electron transfer mediated by osmium complexes. Application in the determination of extremely low levels of glucose
title_fullStr Continuous-flow/stopped-flow system incorporating a rotating bioreactor based on a double redox catalytic cycle and electron transfer mediated by osmium complexes. Application in the determination of extremely low levels of glucose
title_full_unstemmed Continuous-flow/stopped-flow system incorporating a rotating bioreactor based on a double redox catalytic cycle and electron transfer mediated by osmium complexes. Application in the determination of extremely low levels of glucose
title_sort Continuous-flow/stopped-flow system incorporating a rotating bioreactor based on a double redox catalytic cycle and electron transfer mediated by osmium complexes. Application in the determination of extremely low levels of glucose
dc.creator.none.fl_str_mv Salinas, Eloy
Morcillo, Pablo
Sanz Ferramola, Maria Isabel
Raba, Julio
author Salinas, Eloy
author_facet Salinas, Eloy
Morcillo, Pablo
Sanz Ferramola, Maria Isabel
Raba, Julio
author_role author
author2 Morcillo, Pablo
Sanz Ferramola, Maria Isabel
Raba, Julio
author2_role author
author
author
dc.subject.none.fl_str_mv ROTATING BIOREACTOR
CATALYTIC CYCLE
ELECTRON TRANSFER
OSMIUM COMPLEXES
LEVELS OF GLUCOSE
topic ROTATING BIOREACTOR
CATALYTIC CYCLE
ELECTRON TRANSFER
OSMIUM COMPLEXES
LEVELS OF GLUCOSE
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.4
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv The high sensitivity that can be attained by enzymatic amplification via substrate cycling and mediated by the redox polymer [Os(bpy)2ClPyCH2NHpoly( allylamine) (Os-PAA), has been verified by on-line interfacing of a rotating bioreactor and continuous-flow=stopped-flow=continuous-flow processing system. The determination of glucose was possible with a limit of detection of 20 fmol L1 in the processing of as many as 30 samples per hour. Determination at such low levels is of interest in several situations encountered in fermentation biotechnology and clinical chemistry, and for the determination in culture broths; it illustrates the capabilities of the proposed approach. Glucose oxidase and Os-PAA were covalently immobilized on a glassy carbon electrode surface (upper cell body), Glucose dehydrogenase [EC 1.1.1.47] was immobilized on a disk that can be rotated. Substrate cycling was realized via NADH=NADþ that, in conjunction with glucose dehydrogenase, regenerates glucose, the substrate in the glucose oxidase-catalyzed reaction.
Fil: Salinas, Eloy. Universidad Nacional de San Luis; Argentina
Fil: Morcillo, Pablo. Universidad Nacional de San Luis; Argentina
Fil: Sanz Ferramola, Maria Isabel. Universidad Nacional de San Luis; Argentina
Fil: Raba, Julio. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - San Luis. Instituto de Química de San Luis. Universidad Nacional de San Luis. Facultad de Química, Bioquímica y Farmacia. Instituto de Química de San Luis; Argentina
description The high sensitivity that can be attained by enzymatic amplification via substrate cycling and mediated by the redox polymer [Os(bpy)2ClPyCH2NHpoly( allylamine) (Os-PAA), has been verified by on-line interfacing of a rotating bioreactor and continuous-flow=stopped-flow=continuous-flow processing system. The determination of glucose was possible with a limit of detection of 20 fmol L1 in the processing of as many as 30 samples per hour. Determination at such low levels is of interest in several situations encountered in fermentation biotechnology and clinical chemistry, and for the determination in culture broths; it illustrates the capabilities of the proposed approach. Glucose oxidase and Os-PAA were covalently immobilized on a glassy carbon electrode surface (upper cell body), Glucose dehydrogenase [EC 1.1.1.47] was immobilized on a disk that can be rotated. Substrate cycling was realized via NADH=NADþ that, in conjunction with glucose dehydrogenase, regenerates glucose, the substrate in the glucose oxidase-catalyzed reaction.
publishDate 2002
dc.date.none.fl_str_mv 2002-02
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/102178
Salinas, Eloy; Morcillo, Pablo; Sanz Ferramola, Maria Isabel; Raba, Julio; Continuous-flow/stopped-flow system incorporating a rotating bioreactor based on a double redox catalytic cycle and electron transfer mediated by osmium complexes. Application in the determination of extremely low levels of glucose; Taylor & Francis; Instrumentation Science And Technology; 30; 3; 2-2002; 281-293
1073-9149
1525-6030
CONICET Digital
CONICET
url http://hdl.handle.net/11336/102178
identifier_str_mv Salinas, Eloy; Morcillo, Pablo; Sanz Ferramola, Maria Isabel; Raba, Julio; Continuous-flow/stopped-flow system incorporating a rotating bioreactor based on a double redox catalytic cycle and electron transfer mediated by osmium complexes. Application in the determination of extremely low levels of glucose; Taylor & Francis; Instrumentation Science And Technology; 30; 3; 2-2002; 281-293
1073-9149
1525-6030
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://www.tandfonline.com/doi/abs/10.1081/CI-120013507
info:eu-repo/semantics/altIdentifier/doi/10.1081/CI-120013507
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Taylor & Francis
publisher.none.fl_str_mv Taylor & Francis
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
_version_ 1844613926458753024
score 13.070432

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