Use of fluoride for the measurement of bone remodelling in rats and human beings

Autores
Lupo, Maela; Neira, Melina Vanesa; Chulibert, María Eugenia; Lombarte, Mercedes
Año de publicación
2020
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
We examined a new method, based on the retention of fluoride (F), for estimating bone remodelling in rats and human beings. We tested the validity of the new methodology by comparing normal Sprague Dawley rats (Controls, n=20) to ovariectomized rats with high bone remodelling (OVX, n=20). We found that the mean±SD for both bone formation (BF) and bone resorption (BR) in the OVX rats were significantly higher (p<0.05, Student's t-test) than in the Controls (Controls BF=10.28±4.54, OVX BF=20.33±19.00; Controls BR=8.18±4.86, OVX BR=18.41±17.93 nmole F/min). The new method was also tested in human volunteers (n=10) and found not to have any side effects. Three of the advantages of the new technique, in comparison to the use of bone remodelling markers, were: (i) BF and BR can be measured simultaneously, (ii) the real rates of BF and BR are measured, and (iii) it is a low cost technique. The new method was validated and found to be useful for estimating bone remodelling in both rats and human beings
Fil: Lupo, Maela. Universidad Nacional de Rosario. Facultad de Ciencias Médicas. Escuela de Ciencias médicas. Cátedra de Química Biológica; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Rosario; Argentina
Fil: Neira, Melina Vanesa. Universidad Nacional de Rosario. Facultad de Ciencias Médicas. Escuela de Ciencias médicas. Cátedra de Química Biológica; Argentina
Fil: Chulibert, María Eugenia. Universidad Nacional de Rosario. Facultad de Ciencias Médicas. Escuela de Ciencias médicas. Cátedra de Química Biológica; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Rosario; Argentina
Fil: Lombarte, Mercedes. Universidad Nacional de Rosario. Facultad de Ciencias Médicas. Escuela de Ciencias médicas. Cátedra de Química Biológica; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Rosario; Argentina
Materia
Bone marker
Bone remodelling
Mathematical model
Pharmacokinetics
Rat
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/154836
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/154836
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Use of fluoride for the measurement of bone remodelling in rats and human beingsLupo, MaelaNeira, Melina VanesaChulibert, María EugeniaLombarte, MercedesBone markerBone remodellingMathematical modelPharmacokineticsRathttps://purl.org/becyt/ford/3.1https://purl.org/becyt/ford/3We examined a new method, based on the retention of fluoride (F), for estimating bone remodelling in rats and human beings. We tested the validity of the new methodology by comparing normal Sprague Dawley rats (Controls, n=20) to ovariectomized rats with high bone remodelling (OVX, n=20). We found that the mean±SD for both bone formation (BF) and bone resorption (BR) in the OVX rats were significantly higher (p<0.05, Student's t-test) than in the Controls (Controls BF=10.28±4.54, OVX BF=20.33±19.00; Controls BR=8.18±4.86, OVX BR=18.41±17.93 nmole F/min). The new method was also tested in human volunteers (n=10) and found not to have any side effects. Three of the advantages of the new technique, in comparison to the use of bone remodelling markers, were: (i) BF and BR can be measured simultaneously, (ii) the real rates of BF and BR are measured, and (iii) it is a low cost technique. The new method was validated and found to be useful for estimating bone remodelling in both rats and human beingsFil: Lupo, Maela. Universidad Nacional de Rosario. Facultad de Ciencias Médicas. Escuela de Ciencias médicas. Cátedra de Química Biológica; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Rosario; ArgentinaFil: Neira, Melina Vanesa. Universidad Nacional de Rosario. Facultad de Ciencias Médicas. Escuela de Ciencias médicas. Cátedra de Química Biológica; ArgentinaFil: Chulibert, María Eugenia. Universidad Nacional de Rosario. Facultad de Ciencias Médicas. Escuela de Ciencias médicas. Cátedra de Química Biológica; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Rosario; ArgentinaFil: Lombarte, Mercedes. Universidad Nacional de Rosario. Facultad de Ciencias Médicas. Escuela de Ciencias médicas. Cátedra de Química Biológica; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Rosario; ArgentinaInternational Society for Fluoride Research2020-12info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/154836Lupo, Maela; Neira, Melina Vanesa; Chulibert, María Eugenia; Lombarte, Mercedes; Use of fluoride for the measurement of bone remodelling in rats and human beings; International Society for Fluoride Research; Fluoride; 53; 3; 12-2020; 477-4820015-47252253-4083CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://www.fluorideresearch.org/info:eu-repo/semantics/altIdentifier/url/https://www.fluorideresearch.online/epub/files/103.pdfinfo:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-10-22T11:00:21Zoai:ri.conicet.gov.ar:11336/154836instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-10-22 11:00:21.473CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Use of fluoride for the measurement of bone remodelling in rats and human beings
title Use of fluoride for the measurement of bone remodelling in rats and human beings
spellingShingle Use of fluoride for the measurement of bone remodelling in rats and human beings
Lupo, Maela
Bone marker
Bone remodelling
Mathematical model
Pharmacokinetics
Rat
title_short Use of fluoride for the measurement of bone remodelling in rats and human beings
title_full Use of fluoride for the measurement of bone remodelling in rats and human beings
title_fullStr Use of fluoride for the measurement of bone remodelling in rats and human beings
title_full_unstemmed Use of fluoride for the measurement of bone remodelling in rats and human beings
title_sort Use of fluoride for the measurement of bone remodelling in rats and human beings
dc.creator.none.fl_str_mv Lupo, Maela
Neira, Melina Vanesa
Chulibert, María Eugenia
Lombarte, Mercedes
author Lupo, Maela
author_facet Lupo, Maela
Neira, Melina Vanesa
Chulibert, María Eugenia
Lombarte, Mercedes
author_role author
author2 Neira, Melina Vanesa
Chulibert, María Eugenia
Lombarte, Mercedes
author2_role author
author
author
dc.subject.none.fl_str_mv Bone marker
Bone remodelling
Mathematical model
Pharmacokinetics
Rat
topic Bone marker
Bone remodelling
Mathematical model
Pharmacokinetics
Rat
purl_subject.fl_str_mv https://purl.org/becyt/ford/3.1
https://purl.org/becyt/ford/3
dc.description.none.fl_txt_mv We examined a new method, based on the retention of fluoride (F), for estimating bone remodelling in rats and human beings. We tested the validity of the new methodology by comparing normal Sprague Dawley rats (Controls, n=20) to ovariectomized rats with high bone remodelling (OVX, n=20). We found that the mean±SD for both bone formation (BF) and bone resorption (BR) in the OVX rats were significantly higher (p<0.05, Student's t-test) than in the Controls (Controls BF=10.28±4.54, OVX BF=20.33±19.00; Controls BR=8.18±4.86, OVX BR=18.41±17.93 nmole F/min). The new method was also tested in human volunteers (n=10) and found not to have any side effects. Three of the advantages of the new technique, in comparison to the use of bone remodelling markers, were: (i) BF and BR can be measured simultaneously, (ii) the real rates of BF and BR are measured, and (iii) it is a low cost technique. The new method was validated and found to be useful for estimating bone remodelling in both rats and human beings
Fil: Lupo, Maela. Universidad Nacional de Rosario. Facultad de Ciencias Médicas. Escuela de Ciencias médicas. Cátedra de Química Biológica; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Rosario; Argentina
Fil: Neira, Melina Vanesa. Universidad Nacional de Rosario. Facultad de Ciencias Médicas. Escuela de Ciencias médicas. Cátedra de Química Biológica; Argentina
Fil: Chulibert, María Eugenia. Universidad Nacional de Rosario. Facultad de Ciencias Médicas. Escuela de Ciencias médicas. Cátedra de Química Biológica; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Rosario; Argentina
Fil: Lombarte, Mercedes. Universidad Nacional de Rosario. Facultad de Ciencias Médicas. Escuela de Ciencias médicas. Cátedra de Química Biológica; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Rosario; Argentina
description We examined a new method, based on the retention of fluoride (F), for estimating bone remodelling in rats and human beings. We tested the validity of the new methodology by comparing normal Sprague Dawley rats (Controls, n=20) to ovariectomized rats with high bone remodelling (OVX, n=20). We found that the mean±SD for both bone formation (BF) and bone resorption (BR) in the OVX rats were significantly higher (p<0.05, Student's t-test) than in the Controls (Controls BF=10.28±4.54, OVX BF=20.33±19.00; Controls BR=8.18±4.86, OVX BR=18.41±17.93 nmole F/min). The new method was also tested in human volunteers (n=10) and found not to have any side effects. Three of the advantages of the new technique, in comparison to the use of bone remodelling markers, were: (i) BF and BR can be measured simultaneously, (ii) the real rates of BF and BR are measured, and (iii) it is a low cost technique. The new method was validated and found to be useful for estimating bone remodelling in both rats and human beings
publishDate 2020
dc.date.none.fl_str_mv 2020-12
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/154836
Lupo, Maela; Neira, Melina Vanesa; Chulibert, María Eugenia; Lombarte, Mercedes; Use of fluoride for the measurement of bone remodelling in rats and human beings; International Society for Fluoride Research; Fluoride; 53; 3; 12-2020; 477-482
0015-4725
2253-4083
CONICET Digital
CONICET
url http://hdl.handle.net/11336/154836
identifier_str_mv Lupo, Maela; Neira, Melina Vanesa; Chulibert, María Eugenia; Lombarte, Mercedes; Use of fluoride for the measurement of bone remodelling in rats and human beings; International Society for Fluoride Research; Fluoride; 53; 3; 12-2020; 477-482
0015-4725
2253-4083
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://www.fluorideresearch.org/
info:eu-repo/semantics/altIdentifier/url/https://www.fluorideresearch.online/epub/files/103.pdf
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv International Society for Fluoride Research
publisher.none.fl_str_mv International Society for Fluoride Research
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
_version_ 1846781162883645440
score 12.982451

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