Characterization and comparison of strains of Pasteurella multocida associated with cases of progressive atrophic rhinitis and porcine pneumonia in Argentina

Autores
Bessone, Fernando Anibal; Soriano Pérez, María Laura; Zielinski, Gustavo Carlos; Dibárbora, Marina; Conde, María Belén; Cappuccio, Javier Alejandro; Alustiza, Fabrisio Eduardo
Año de publicación
2019
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Pasteurella multocida (Pm) is the causative agent of progressive atrophic rhinitis (PAR) and pneumonicpasteurellosis (PN) in pigs. Pm is a member of the porcine respiratory complex responsible for important economic loss inthe pig industry. This study aimed to characterize the Pm strains recovered from clinical cases of PN and PAR and to elucidate theantibiotic susceptibility profiles of the strains.Materials and Methods: Sixty strains were characterized molecularly by polymerase chain reaction to determine speciesspecific gene, capsular type (A or D), and toxin A production. The agar diffusion method was employed to evaluate antibioticresistance profiles. We found that 65% of strains belonged to capsular type A or D, and 15% of those were positive to toxA gene.The antibiotic susceptibility profiles found were sensitive in decreasing order to: Enrofloxacin, ceftiofur (CTF), ampicillin,tilmicosin (TIL), florfenicol (FFN), spectinomycin (SPC), gentamicin, oxytetracycline (OTC), and trimethoprimsulfamethoxazole (TMS). Strains were resistant in decreasing order to: Lincomycin (LIN), tylosin (TYL), erythromycin(ERY), TMS, SPC, OTC, FFN, TIL, and CTF. The toxA gene was detected in many Pm isolates from pneumonic lungs. Capsule type A or D was the mostfrequently found among the collected isolates. LIN, TYL, and ERY are the drugs which showed higher percentages of resistant isolates.
Fil: Bessone, Fernando Anibal. Instituto Nacional de Tecnología Agropecuaria. Centro Regional Córdoba. Estación Experimental Agropecuaria Marcos Juárez; Argentina
Fil: Soriano Pérez, María Laura. Instituto Nacional de Tecnología Agropecuaria. Centro Regional Córdoba. Estación Experimental Agropecuaria Marcos Juárez; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Zielinski, Gustavo Carlos. Instituto Nacional de Tecnología Agropecuaria. Centro Regional Córdoba. Estación Experimental Agropecuaria Marcos Juárez; Argentina
Fil: Dibárbora, Marina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Instituto Nacional de Tecnología Agropecuaria. Centro Regional Córdoba. Estación Experimental Agropecuaria Marcos Juárez; Argentina
Fil: Conde, María Belén. Instituto Nacional de Tecnología Agropecuaria. Centro Regional Córdoba. Estación Experimental Agropecuaria Marcos Juárez; Argentina
Fil: Cappuccio, Javier Alejandro. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigación en Ciencias Veterinarias y Agronómicas. Instituto de Virología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Alustiza, Fabrisio Eduardo. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas Fisicoquímicas y Naturales. Departamento de Química; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Materia
ANTIBIOTIC SUSCEPTIBILITY PROFILES
MOLECULAR CHARACTERIZATION
PASTEURELLA MULTOCIDA
PIG
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/119294
Acceder
id CONICETDig_5b4cb65913b40fe173a82332982ad0da
oai_identifier_str oai:ri.conicet.gov.ar:11336/119294
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Characterization and comparison of strains of Pasteurella multocida associated with cases of progressive atrophic rhinitis and porcine pneumonia in ArgentinaBessone, Fernando AnibalSoriano Pérez, María LauraZielinski, Gustavo CarlosDibárbora, MarinaConde, María BelénCappuccio, Javier AlejandroAlustiza, Fabrisio EduardoANTIBIOTIC SUSCEPTIBILITY PROFILESMOLECULAR CHARACTERIZATIONPASTEURELLA MULTOCIDAPIGhttps://purl.org/becyt/ford/4.3https://purl.org/becyt/ford/4Pasteurella multocida (Pm) is the causative agent of progressive atrophic rhinitis (PAR) and pneumonicpasteurellosis (PN) in pigs. Pm is a member of the porcine respiratory complex responsible for important economic loss inthe pig industry. This study aimed to characterize the Pm strains recovered from clinical cases of PN and PAR and to elucidate theantibiotic susceptibility profiles of the strains.Materials and Methods: Sixty strains were characterized molecularly by polymerase chain reaction to determine speciesspecific gene, capsular type (A or D), and toxin A production. The agar diffusion method was employed to evaluate antibioticresistance profiles. We found that 65% of strains belonged to capsular type A or D, and 15% of those were positive to toxA gene.The antibiotic susceptibility profiles found were sensitive in decreasing order to: Enrofloxacin, ceftiofur (CTF), ampicillin,tilmicosin (TIL), florfenicol (FFN), spectinomycin (SPC), gentamicin, oxytetracycline (OTC), and trimethoprimsulfamethoxazole (TMS). Strains were resistant in decreasing order to: Lincomycin (LIN), tylosin (TYL), erythromycin(ERY), TMS, SPC, OTC, FFN, TIL, and CTF. The toxA gene was detected in many Pm isolates from pneumonic lungs. Capsule type A or D was the mostfrequently found among the collected isolates. LIN, TYL, and ERY are the drugs which showed higher percentages of resistant isolates.Fil: Bessone, Fernando Anibal. Instituto Nacional de Tecnología Agropecuaria. Centro Regional Córdoba. Estación Experimental Agropecuaria Marcos Juárez; ArgentinaFil: Soriano Pérez, María Laura. Instituto Nacional de Tecnología Agropecuaria. Centro Regional Córdoba. Estación Experimental Agropecuaria Marcos Juárez; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Zielinski, Gustavo Carlos. Instituto Nacional de Tecnología Agropecuaria. Centro Regional Córdoba. Estación Experimental Agropecuaria Marcos Juárez; ArgentinaFil: Dibárbora, Marina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Instituto Nacional de Tecnología Agropecuaria. Centro Regional Córdoba. Estación Experimental Agropecuaria Marcos Juárez; ArgentinaFil: Conde, María Belén. Instituto Nacional de Tecnología Agropecuaria. Centro Regional Córdoba. Estación Experimental Agropecuaria Marcos Juárez; ArgentinaFil: Cappuccio, Javier Alejandro. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigación en Ciencias Veterinarias y Agronómicas. Instituto de Virología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Alustiza, Fabrisio Eduardo. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas Fisicoquímicas y Naturales. Departamento de Química; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaVeterinary World2019-03info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/119294Bessone, Fernando Anibal; Soriano Pérez, María Laura; Zielinski, Gustavo Carlos; Dibárbora, Marina; Conde, María Belén; et al.; Characterization and comparison of strains of Pasteurella multocida associated with cases of progressive atrophic rhinitis and porcine pneumonia in Argentina; Veterinary World; Veterinary World; 12; 3; 3-2019; 434-4390972-89882231-0916CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://pubmed.ncbi.nlm.nih.gov/31089314/info:eu-repo/semantics/altIdentifier/doi/10.14202/vetworld.2019.434-439info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T10:08:25Zoai:ri.conicet.gov.ar:11336/119294instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 10:08:26.138CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Characterization and comparison of strains of Pasteurella multocida associated with cases of progressive atrophic rhinitis and porcine pneumonia in Argentina
title Characterization and comparison of strains of Pasteurella multocida associated with cases of progressive atrophic rhinitis and porcine pneumonia in Argentina
spellingShingle Characterization and comparison of strains of Pasteurella multocida associated with cases of progressive atrophic rhinitis and porcine pneumonia in Argentina
Bessone, Fernando Anibal
ANTIBIOTIC SUSCEPTIBILITY PROFILES
MOLECULAR CHARACTERIZATION
PASTEURELLA MULTOCIDA
PIG
title_short Characterization and comparison of strains of Pasteurella multocida associated with cases of progressive atrophic rhinitis and porcine pneumonia in Argentina
title_full Characterization and comparison of strains of Pasteurella multocida associated with cases of progressive atrophic rhinitis and porcine pneumonia in Argentina
title_fullStr Characterization and comparison of strains of Pasteurella multocida associated with cases of progressive atrophic rhinitis and porcine pneumonia in Argentina
title_full_unstemmed Characterization and comparison of strains of Pasteurella multocida associated with cases of progressive atrophic rhinitis and porcine pneumonia in Argentina
title_sort Characterization and comparison of strains of Pasteurella multocida associated with cases of progressive atrophic rhinitis and porcine pneumonia in Argentina
dc.creator.none.fl_str_mv Bessone, Fernando Anibal
Soriano Pérez, María Laura
Zielinski, Gustavo Carlos
Dibárbora, Marina
Conde, María Belén
Cappuccio, Javier Alejandro
Alustiza, Fabrisio Eduardo
author Bessone, Fernando Anibal
author_facet Bessone, Fernando Anibal
Soriano Pérez, María Laura
Zielinski, Gustavo Carlos
Dibárbora, Marina
Conde, María Belén
Cappuccio, Javier Alejandro
Alustiza, Fabrisio Eduardo
author_role author
author2 Soriano Pérez, María Laura
Zielinski, Gustavo Carlos
Dibárbora, Marina
Conde, María Belén
Cappuccio, Javier Alejandro
Alustiza, Fabrisio Eduardo
author2_role author
author
author
author
author
author
dc.subject.none.fl_str_mv ANTIBIOTIC SUSCEPTIBILITY PROFILES
MOLECULAR CHARACTERIZATION
PASTEURELLA MULTOCIDA
PIG
topic ANTIBIOTIC SUSCEPTIBILITY PROFILES
MOLECULAR CHARACTERIZATION
PASTEURELLA MULTOCIDA
PIG
purl_subject.fl_str_mv https://purl.org/becyt/ford/4.3
https://purl.org/becyt/ford/4
dc.description.none.fl_txt_mv Pasteurella multocida (Pm) is the causative agent of progressive atrophic rhinitis (PAR) and pneumonicpasteurellosis (PN) in pigs. Pm is a member of the porcine respiratory complex responsible for important economic loss inthe pig industry. This study aimed to characterize the Pm strains recovered from clinical cases of PN and PAR and to elucidate theantibiotic susceptibility profiles of the strains.Materials and Methods: Sixty strains were characterized molecularly by polymerase chain reaction to determine speciesspecific gene, capsular type (A or D), and toxin A production. The agar diffusion method was employed to evaluate antibioticresistance profiles. We found that 65% of strains belonged to capsular type A or D, and 15% of those were positive to toxA gene.The antibiotic susceptibility profiles found were sensitive in decreasing order to: Enrofloxacin, ceftiofur (CTF), ampicillin,tilmicosin (TIL), florfenicol (FFN), spectinomycin (SPC), gentamicin, oxytetracycline (OTC), and trimethoprimsulfamethoxazole (TMS). Strains were resistant in decreasing order to: Lincomycin (LIN), tylosin (TYL), erythromycin(ERY), TMS, SPC, OTC, FFN, TIL, and CTF. The toxA gene was detected in many Pm isolates from pneumonic lungs. Capsule type A or D was the mostfrequently found among the collected isolates. LIN, TYL, and ERY are the drugs which showed higher percentages of resistant isolates.
Fil: Bessone, Fernando Anibal. Instituto Nacional de Tecnología Agropecuaria. Centro Regional Córdoba. Estación Experimental Agropecuaria Marcos Juárez; Argentina
Fil: Soriano Pérez, María Laura. Instituto Nacional de Tecnología Agropecuaria. Centro Regional Córdoba. Estación Experimental Agropecuaria Marcos Juárez; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Zielinski, Gustavo Carlos. Instituto Nacional de Tecnología Agropecuaria. Centro Regional Córdoba. Estación Experimental Agropecuaria Marcos Juárez; Argentina
Fil: Dibárbora, Marina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Instituto Nacional de Tecnología Agropecuaria. Centro Regional Córdoba. Estación Experimental Agropecuaria Marcos Juárez; Argentina
Fil: Conde, María Belén. Instituto Nacional de Tecnología Agropecuaria. Centro Regional Córdoba. Estación Experimental Agropecuaria Marcos Juárez; Argentina
Fil: Cappuccio, Javier Alejandro. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigación en Ciencias Veterinarias y Agronómicas. Instituto de Virología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Alustiza, Fabrisio Eduardo. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas Fisicoquímicas y Naturales. Departamento de Química; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
description Pasteurella multocida (Pm) is the causative agent of progressive atrophic rhinitis (PAR) and pneumonicpasteurellosis (PN) in pigs. Pm is a member of the porcine respiratory complex responsible for important economic loss inthe pig industry. This study aimed to characterize the Pm strains recovered from clinical cases of PN and PAR and to elucidate theantibiotic susceptibility profiles of the strains.Materials and Methods: Sixty strains were characterized molecularly by polymerase chain reaction to determine speciesspecific gene, capsular type (A or D), and toxin A production. The agar diffusion method was employed to evaluate antibioticresistance profiles. We found that 65% of strains belonged to capsular type A or D, and 15% of those were positive to toxA gene.The antibiotic susceptibility profiles found were sensitive in decreasing order to: Enrofloxacin, ceftiofur (CTF), ampicillin,tilmicosin (TIL), florfenicol (FFN), spectinomycin (SPC), gentamicin, oxytetracycline (OTC), and trimethoprimsulfamethoxazole (TMS). Strains were resistant in decreasing order to: Lincomycin (LIN), tylosin (TYL), erythromycin(ERY), TMS, SPC, OTC, FFN, TIL, and CTF. The toxA gene was detected in many Pm isolates from pneumonic lungs. Capsule type A or D was the mostfrequently found among the collected isolates. LIN, TYL, and ERY are the drugs which showed higher percentages of resistant isolates.
publishDate 2019
dc.date.none.fl_str_mv 2019-03
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/119294
Bessone, Fernando Anibal; Soriano Pérez, María Laura; Zielinski, Gustavo Carlos; Dibárbora, Marina; Conde, María Belén; et al.; Characterization and comparison of strains of Pasteurella multocida associated with cases of progressive atrophic rhinitis and porcine pneumonia in Argentina; Veterinary World; Veterinary World; 12; 3; 3-2019; 434-439
0972-8988
2231-0916
CONICET Digital
CONICET
url http://hdl.handle.net/11336/119294
identifier_str_mv Bessone, Fernando Anibal; Soriano Pérez, María Laura; Zielinski, Gustavo Carlos; Dibárbora, Marina; Conde, María Belén; et al.; Characterization and comparison of strains of Pasteurella multocida associated with cases of progressive atrophic rhinitis and porcine pneumonia in Argentina; Veterinary World; Veterinary World; 12; 3; 3-2019; 434-439
0972-8988
2231-0916
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://pubmed.ncbi.nlm.nih.gov/31089314/
info:eu-repo/semantics/altIdentifier/doi/10.14202/vetworld.2019.434-439
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Veterinary World
publisher.none.fl_str_mv Veterinary World
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.070432

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