Biochemical action of medroxyprogesterone acetate on ovariectomized obese rats

Autores
Cutini, Pablo Hernan; Cepeda, Sabrina Belén; Sandoval, Marisa Julia; Massheimer, Virginia Laura
Año de publicación
2023
Idioma
inglés
Tipo de recurso
documento de conferencia
Estado
versión publicada
Descripción
Obesity, cardiovascular and bone diseases are multifactorial clinical entities that often coexist in postmenopausal women. Medroxyprogesterone acetate (MPA) is usually employed in hormone replacement therapy. Previous studies carried out in our laboratory at vascular level, demonstrated a divergent action between native progesterone (Pg) and its synthetic analogue MPA, evidenced by an oxidative, proatherogenic and prothrombotic effect, and an impairment in aortic recovery elicited by MPA in contrast to Pg action. VEGF is a key mediator in vascular and bone repair. The aim of this work was to evaluate the effect of MPA on VEGF production on vascular and bone tissue, as wells as the impact on adiposity oxidative stress in a model of obesity and hypoestrogenism. To that end, aortic endothelial cells (EC), retroperitoneal adipose tissue (AT) and femoral diaphysis (FD) were isolated from ovariectomized Wistar rats fed with a high-fat diet (27%) for 10 weeks. For in vitro treatments, AT explants, FD and EC cultures were incubated with 10 nM MPA for 20 h. Control group received vehicle only (isopropanol). Treatment of EC culture with MPA induced a significant reduction in VEGF synthesis (34% vs control, P<0.0001, ELISA Kit). This inhibition of growth factor synthesis was also observed in FD after exposure to the synthetic progestin (33% vs control, P<0.01). Both results suggest an impair action on tissue repair capability. When AT was incubated with MPA, reduction in oxidative stress was detected. Significant decrease in H2O2 release from AT isolated form obese rats was observed (2725 ± 432 vs 2043 ± 69 nmol H2O2/g of tissue, control vs MPA respectively, P<0.05, fluorometric assay). In addition, after treatment of AT with MPA, leptin levels increased in the culture medium (28% vs control, P<0.025, ELISA kit). The results presented suggest that, under hypoestrogenism and obesity, MPA could exert negative or positive effects according to the target tissue involved.
Fil: Cutini, Pablo Hernan. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Ciencias Biológicas y Biomédicas del Sur. Universidad Nacional del Sur. Departamento de Biología, Bioquímica y Farmacia. Instituto de Ciencias Biológicas y Biomédicas del Sur; Argentina
Fil: Cepeda, Sabrina Belén. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Ciencias Biológicas y Biomédicas del Sur. Universidad Nacional del Sur. Departamento de Biología, Bioquímica y Farmacia. Instituto de Ciencias Biológicas y Biomédicas del Sur; Argentina
Fil: Sandoval, Marisa Julia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Ciencias Biológicas y Biomédicas del Sur. Universidad Nacional del Sur. Departamento de Biología, Bioquímica y Farmacia. Instituto de Ciencias Biológicas y Biomédicas del Sur; Argentina
Fil: Massheimer, Virginia Laura. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Ciencias Biológicas y Biomédicas del Sur. Universidad Nacional del Sur. Departamento de Biología, Bioquímica y Farmacia. Instituto de Ciencias Biológicas y Biomédicas del Sur; Argentina
LXVIII Reunión Anual de la Sociedad Argentina de Investigación Clínica; XXV Jornadas Anuales de la Sociedad Argentina de Biología; LV Reunión Anual de la Asociación Argentina de Farmacología Experimental y VIII Reunión Científica Regional de la Asociación Argentina de Ciencia y Tecnología de Animales de Laboratorio
Mar del Plata
Argentina
Sociedad Argentina de Investigación Clínica
Sociedad Argentina de Biología
Asociación Argentina de Ciencia y Tecnología de Animales de Laboratorio
Asociación Argentina de Farmacología Experimental
Materia
MEDROXYPROGESTERONE ACETATE
OBESIDAD
HYPOESTROGENISM
ADIPOSE TISSUE
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/238274
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/238274
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Biochemical action of medroxyprogesterone acetate on ovariectomized obese ratsCutini, Pablo HernanCepeda, Sabrina BelénSandoval, Marisa JuliaMassheimer, Virginia LauraMEDROXYPROGESTERONE ACETATEOBESIDADHYPOESTROGENISMADIPOSE TISSUEhttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1Obesity, cardiovascular and bone diseases are multifactorial clinical entities that often coexist in postmenopausal women. Medroxyprogesterone acetate (MPA) is usually employed in hormone replacement therapy. Previous studies carried out in our laboratory at vascular level, demonstrated a divergent action between native progesterone (Pg) and its synthetic analogue MPA, evidenced by an oxidative, proatherogenic and prothrombotic effect, and an impairment in aortic recovery elicited by MPA in contrast to Pg action. VEGF is a key mediator in vascular and bone repair. The aim of this work was to evaluate the effect of MPA on VEGF production on vascular and bone tissue, as wells as the impact on adiposity oxidative stress in a model of obesity and hypoestrogenism. To that end, aortic endothelial cells (EC), retroperitoneal adipose tissue (AT) and femoral diaphysis (FD) were isolated from ovariectomized Wistar rats fed with a high-fat diet (27%) for 10 weeks. For in vitro treatments, AT explants, FD and EC cultures were incubated with 10 nM MPA for 20 h. Control group received vehicle only (isopropanol). Treatment of EC culture with MPA induced a significant reduction in VEGF synthesis (34% vs control, P<0.0001, ELISA Kit). This inhibition of growth factor synthesis was also observed in FD after exposure to the synthetic progestin (33% vs control, P<0.01). Both results suggest an impair action on tissue repair capability. When AT was incubated with MPA, reduction in oxidative stress was detected. Significant decrease in H2O2 release from AT isolated form obese rats was observed (2725 ± 432 vs 2043 ± 69 nmol H2O2/g of tissue, control vs MPA respectively, P<0.05, fluorometric assay). In addition, after treatment of AT with MPA, leptin levels increased in the culture medium (28% vs control, P<0.025, ELISA kit). The results presented suggest that, under hypoestrogenism and obesity, MPA could exert negative or positive effects according to the target tissue involved.Fil: Cutini, Pablo Hernan. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Ciencias Biológicas y Biomédicas del Sur. Universidad Nacional del Sur. Departamento de Biología, Bioquímica y Farmacia. Instituto de Ciencias Biológicas y Biomédicas del Sur; ArgentinaFil: Cepeda, Sabrina Belén. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Ciencias Biológicas y Biomédicas del Sur. Universidad Nacional del Sur. Departamento de Biología, Bioquímica y Farmacia. Instituto de Ciencias Biológicas y Biomédicas del Sur; ArgentinaFil: Sandoval, Marisa Julia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Ciencias Biológicas y Biomédicas del Sur. Universidad Nacional del Sur. Departamento de Biología, Bioquímica y Farmacia. Instituto de Ciencias Biológicas y Biomédicas del Sur; ArgentinaFil: Massheimer, Virginia Laura. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Ciencias Biológicas y Biomédicas del Sur. Universidad Nacional del Sur. Departamento de Biología, Bioquímica y Farmacia. Instituto de Ciencias Biológicas y Biomédicas del Sur; ArgentinaLXVIII Reunión Anual de la Sociedad Argentina de Investigación Clínica; XXV Jornadas Anuales de la Sociedad Argentina de Biología; LV Reunión Anual de la Asociación Argentina de Farmacología Experimental y VIII Reunión Científica Regional de la Asociación Argentina de Ciencia y Tecnología de Animales de LaboratorioMar del PlataArgentinaSociedad Argentina de Investigación ClínicaSociedad Argentina de BiologíaAsociación Argentina de Ciencia y Tecnología de Animales de LaboratorioAsociación Argentina de Farmacología ExperimentalFundación Revista Medicina2023info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/conferenceObjectReuniónJournalhttp://purl.org/coar/resource_type/c_5794info:ar-repo/semantics/documentoDeConferenciaapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/238274Biochemical action of medroxyprogesterone acetate on ovariectomized obese rats; LXVIII Reunión Anual de la Sociedad Argentina de Investigación Clínica; XXV Jornadas Anuales de la Sociedad Argentina de Biología; LV Reunión Anual de la Asociación Argentina de Farmacología Experimental y VIII Reunión Científica Regional de la Asociación Argentina de Ciencia y Tecnología de Animales de Laboratorio; Mar del Plata; Argentina; 2023; 1-61669-9106CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://medicinabuenosaires.com/revistas/vol83-23/s5/1s5.pdfNacionalinfo:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-10-22T11:22:24Zoai:ri.conicet.gov.ar:11336/238274instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-10-22 11:22:24.787CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Biochemical action of medroxyprogesterone acetate on ovariectomized obese rats
title Biochemical action of medroxyprogesterone acetate on ovariectomized obese rats
spellingShingle Biochemical action of medroxyprogesterone acetate on ovariectomized obese rats
Cutini, Pablo Hernan
MEDROXYPROGESTERONE ACETATE
OBESIDAD
HYPOESTROGENISM
ADIPOSE TISSUE
title_short Biochemical action of medroxyprogesterone acetate on ovariectomized obese rats
title_full Biochemical action of medroxyprogesterone acetate on ovariectomized obese rats
title_fullStr Biochemical action of medroxyprogesterone acetate on ovariectomized obese rats
title_full_unstemmed Biochemical action of medroxyprogesterone acetate on ovariectomized obese rats
title_sort Biochemical action of medroxyprogesterone acetate on ovariectomized obese rats
dc.creator.none.fl_str_mv Cutini, Pablo Hernan
Cepeda, Sabrina Belén
Sandoval, Marisa Julia
Massheimer, Virginia Laura
author Cutini, Pablo Hernan
author_facet Cutini, Pablo Hernan
Cepeda, Sabrina Belén
Sandoval, Marisa Julia
Massheimer, Virginia Laura
author_role author
author2 Cepeda, Sabrina Belén
Sandoval, Marisa Julia
Massheimer, Virginia Laura
author2_role author
author
author
dc.subject.none.fl_str_mv MEDROXYPROGESTERONE ACETATE
OBESIDAD
HYPOESTROGENISM
ADIPOSE TISSUE
topic MEDROXYPROGESTERONE ACETATE
OBESIDAD
HYPOESTROGENISM
ADIPOSE TISSUE
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv Obesity, cardiovascular and bone diseases are multifactorial clinical entities that often coexist in postmenopausal women. Medroxyprogesterone acetate (MPA) is usually employed in hormone replacement therapy. Previous studies carried out in our laboratory at vascular level, demonstrated a divergent action between native progesterone (Pg) and its synthetic analogue MPA, evidenced by an oxidative, proatherogenic and prothrombotic effect, and an impairment in aortic recovery elicited by MPA in contrast to Pg action. VEGF is a key mediator in vascular and bone repair. The aim of this work was to evaluate the effect of MPA on VEGF production on vascular and bone tissue, as wells as the impact on adiposity oxidative stress in a model of obesity and hypoestrogenism. To that end, aortic endothelial cells (EC), retroperitoneal adipose tissue (AT) and femoral diaphysis (FD) were isolated from ovariectomized Wistar rats fed with a high-fat diet (27%) for 10 weeks. For in vitro treatments, AT explants, FD and EC cultures were incubated with 10 nM MPA for 20 h. Control group received vehicle only (isopropanol). Treatment of EC culture with MPA induced a significant reduction in VEGF synthesis (34% vs control, P<0.0001, ELISA Kit). This inhibition of growth factor synthesis was also observed in FD after exposure to the synthetic progestin (33% vs control, P<0.01). Both results suggest an impair action on tissue repair capability. When AT was incubated with MPA, reduction in oxidative stress was detected. Significant decrease in H2O2 release from AT isolated form obese rats was observed (2725 ± 432 vs 2043 ± 69 nmol H2O2/g of tissue, control vs MPA respectively, P<0.05, fluorometric assay). In addition, after treatment of AT with MPA, leptin levels increased in the culture medium (28% vs control, P<0.025, ELISA kit). The results presented suggest that, under hypoestrogenism and obesity, MPA could exert negative or positive effects according to the target tissue involved.
Fil: Cutini, Pablo Hernan. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Ciencias Biológicas y Biomédicas del Sur. Universidad Nacional del Sur. Departamento de Biología, Bioquímica y Farmacia. Instituto de Ciencias Biológicas y Biomédicas del Sur; Argentina
Fil: Cepeda, Sabrina Belén. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Ciencias Biológicas y Biomédicas del Sur. Universidad Nacional del Sur. Departamento de Biología, Bioquímica y Farmacia. Instituto de Ciencias Biológicas y Biomédicas del Sur; Argentina
Fil: Sandoval, Marisa Julia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Ciencias Biológicas y Biomédicas del Sur. Universidad Nacional del Sur. Departamento de Biología, Bioquímica y Farmacia. Instituto de Ciencias Biológicas y Biomédicas del Sur; Argentina
Fil: Massheimer, Virginia Laura. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Ciencias Biológicas y Biomédicas del Sur. Universidad Nacional del Sur. Departamento de Biología, Bioquímica y Farmacia. Instituto de Ciencias Biológicas y Biomédicas del Sur; Argentina
LXVIII Reunión Anual de la Sociedad Argentina de Investigación Clínica; XXV Jornadas Anuales de la Sociedad Argentina de Biología; LV Reunión Anual de la Asociación Argentina de Farmacología Experimental y VIII Reunión Científica Regional de la Asociación Argentina de Ciencia y Tecnología de Animales de Laboratorio
Mar del Plata
Argentina
Sociedad Argentina de Investigación Clínica
Sociedad Argentina de Biología
Asociación Argentina de Ciencia y Tecnología de Animales de Laboratorio
Asociación Argentina de Farmacología Experimental
description Obesity, cardiovascular and bone diseases are multifactorial clinical entities that often coexist in postmenopausal women. Medroxyprogesterone acetate (MPA) is usually employed in hormone replacement therapy. Previous studies carried out in our laboratory at vascular level, demonstrated a divergent action between native progesterone (Pg) and its synthetic analogue MPA, evidenced by an oxidative, proatherogenic and prothrombotic effect, and an impairment in aortic recovery elicited by MPA in contrast to Pg action. VEGF is a key mediator in vascular and bone repair. The aim of this work was to evaluate the effect of MPA on VEGF production on vascular and bone tissue, as wells as the impact on adiposity oxidative stress in a model of obesity and hypoestrogenism. To that end, aortic endothelial cells (EC), retroperitoneal adipose tissue (AT) and femoral diaphysis (FD) were isolated from ovariectomized Wistar rats fed with a high-fat diet (27%) for 10 weeks. For in vitro treatments, AT explants, FD and EC cultures were incubated with 10 nM MPA for 20 h. Control group received vehicle only (isopropanol). Treatment of EC culture with MPA induced a significant reduction in VEGF synthesis (34% vs control, P<0.0001, ELISA Kit). This inhibition of growth factor synthesis was also observed in FD after exposure to the synthetic progestin (33% vs control, P<0.01). Both results suggest an impair action on tissue repair capability. When AT was incubated with MPA, reduction in oxidative stress was detected. Significant decrease in H2O2 release from AT isolated form obese rats was observed (2725 ± 432 vs 2043 ± 69 nmol H2O2/g of tissue, control vs MPA respectively, P<0.05, fluorometric assay). In addition, after treatment of AT with MPA, leptin levels increased in the culture medium (28% vs control, P<0.025, ELISA kit). The results presented suggest that, under hypoestrogenism and obesity, MPA could exert negative or positive effects according to the target tissue involved.
publishDate 2023
dc.date.none.fl_str_mv 2023
dc.type.none.fl_str_mv info:eu-repo/semantics/publishedVersion
info:eu-repo/semantics/conferenceObject
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http://purl.org/coar/resource_type/c_5794
info:ar-repo/semantics/documentoDeConferencia
status_str publishedVersion
format conferenceObject
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/238274
Biochemical action of medroxyprogesterone acetate on ovariectomized obese rats; LXVIII Reunión Anual de la Sociedad Argentina de Investigación Clínica; XXV Jornadas Anuales de la Sociedad Argentina de Biología; LV Reunión Anual de la Asociación Argentina de Farmacología Experimental y VIII Reunión Científica Regional de la Asociación Argentina de Ciencia y Tecnología de Animales de Laboratorio; Mar del Plata; Argentina; 2023; 1-6
1669-9106
CONICET Digital
CONICET
url http://hdl.handle.net/11336/238274
identifier_str_mv Biochemical action of medroxyprogesterone acetate on ovariectomized obese rats; LXVIII Reunión Anual de la Sociedad Argentina de Investigación Clínica; XXV Jornadas Anuales de la Sociedad Argentina de Biología; LV Reunión Anual de la Asociación Argentina de Farmacología Experimental y VIII Reunión Científica Regional de la Asociación Argentina de Ciencia y Tecnología de Animales de Laboratorio; Mar del Plata; Argentina; 2023; 1-6
1669-9106
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://medicinabuenosaires.com/revistas/vol83-23/s5/1s5.pdf
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dc.publisher.none.fl_str_mv Fundación Revista Medicina
publisher.none.fl_str_mv Fundación Revista Medicina
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