Polar protein Wag31 both activates and inhibits cell wall metabolism at the poles and septum

Autores
Habibi Arejan, Neda; Ensinck, Delfina; Diacovich, Lautaro; Patel, Parthvi Bharatkumar; Quintanilla, Samantha Y.; Emami Saleh, Arash; Gramajo, Hugo Cesar; Boutte, Cara C.
Año de publicación
2023
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Mycobacterial cell elongation occurs at the cell poles; however, it is not clear how cell wall insertion is restricted to the pole or how it is organized. Wag31 is a pole-localized cytoplasmic protein that is essential for polar growth, but its molecular function has not been described. In this study we used alanine scanning mutagenesis to identify Wag31 residues involved in cell morphogenesis. Our data show that Wag31 helps to control proper septation as well as new and old pole elongation. We have identified key amino acid residues involved in these essential functions. Enzyme assays revealed that Wag31 interacts with lipid metabolism by modulating acyl-CoA carboxylase (ACCase) activity. We show that Wag31 does not control polar growth by regulating the localization of cell wall precursor enzymes to the Intracellular Membrane Domain, and we also demonstrate that phosphorylation of Wag31 does not substantively regulate peptidoglycan metabolism. This work establishes new regulatory functions of Wag31 in the mycobacterial cell cycle and clarifies the need for new molecular models of Wag31 function.
Fil: Habibi Arejan, Neda. University of Texas; Estados Unidos
Fil: Ensinck, Delfina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Rosario. Instituto de Biología Molecular y Celular de Rosario. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Instituto de Biología Molecular y Celular de Rosario; Argentina
Fil: Diacovich, Lautaro. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Rosario. Instituto de Biología Molecular y Celular de Rosario. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Instituto de Biología Molecular y Celular de Rosario; Argentina
Fil: Patel, Parthvi Bharatkumar. University of Texas; Estados Unidos
Fil: Quintanilla, Samantha Y.. University of Texas; Estados Unidos
Fil: Emami Saleh, Arash. University of Texas; Estados Unidos
Fil: Gramajo, Hugo Cesar. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Rosario. Instituto de Biología Molecular y Celular de Rosario. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Instituto de Biología Molecular y Celular de Rosario; Argentina
Fil: Boutte, Cara C.. University of Texas; Estados Unidos
Materia
ACCASE COMPLEX
DIVIVA, INTRACELLULAR MEMBRANE DOMAIN (IMD)
FTSI
MURG
MYCOBACTERIUM
PETIDOGLYCAN SYNTHESIS
POLAR GROWTH REGULATION
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/233260
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/233260
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Polar protein Wag31 both activates and inhibits cell wall metabolism at the poles and septumHabibi Arejan, NedaEnsinck, DelfinaDiacovich, LautaroPatel, Parthvi BharatkumarQuintanilla, Samantha Y.Emami Saleh, ArashGramajo, Hugo CesarBoutte, Cara C.ACCASE COMPLEXDIVIVA, INTRACELLULAR MEMBRANE DOMAIN (IMD)FTSIMURGMYCOBACTERIUMPETIDOGLYCAN SYNTHESISPOLAR GROWTH REGULATIONhttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1https://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1Mycobacterial cell elongation occurs at the cell poles; however, it is not clear how cell wall insertion is restricted to the pole or how it is organized. Wag31 is a pole-localized cytoplasmic protein that is essential for polar growth, but its molecular function has not been described. In this study we used alanine scanning mutagenesis to identify Wag31 residues involved in cell morphogenesis. Our data show that Wag31 helps to control proper septation as well as new and old pole elongation. We have identified key amino acid residues involved in these essential functions. Enzyme assays revealed that Wag31 interacts with lipid metabolism by modulating acyl-CoA carboxylase (ACCase) activity. We show that Wag31 does not control polar growth by regulating the localization of cell wall precursor enzymes to the Intracellular Membrane Domain, and we also demonstrate that phosphorylation of Wag31 does not substantively regulate peptidoglycan metabolism. This work establishes new regulatory functions of Wag31 in the mycobacterial cell cycle and clarifies the need for new molecular models of Wag31 function.Fil: Habibi Arejan, Neda. University of Texas; Estados UnidosFil: Ensinck, Delfina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Rosario. Instituto de Biología Molecular y Celular de Rosario. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Instituto de Biología Molecular y Celular de Rosario; ArgentinaFil: Diacovich, Lautaro. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Rosario. Instituto de Biología Molecular y Celular de Rosario. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Instituto de Biología Molecular y Celular de Rosario; ArgentinaFil: Patel, Parthvi Bharatkumar. University of Texas; Estados UnidosFil: Quintanilla, Samantha Y.. University of Texas; Estados UnidosFil: Emami Saleh, Arash. University of Texas; Estados UnidosFil: Gramajo, Hugo Cesar. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Rosario. Instituto de Biología Molecular y Celular de Rosario. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Instituto de Biología Molecular y Celular de Rosario; ArgentinaFil: Boutte, Cara C.. University of Texas; Estados UnidosFrontiers Media2023-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/233260Habibi Arejan, Neda; Ensinck, Delfina; Diacovich, Lautaro; Patel, Parthvi Bharatkumar; Quintanilla, Samantha Y.; et al.; Polar protein Wag31 both activates and inhibits cell wall metabolism at the poles and septum; Frontiers Media; Frontiers in Microbiology; 13; 1-2023; 1-151664-302XCONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://www.frontiersin.org/articles/10.3389/fmicb.2022.1085918/fullinfo:eu-repo/semantics/altIdentifier/doi/10.3389/fmicb.2022.1085918info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-10-15T14:35:23Zoai:ri.conicet.gov.ar:11336/233260instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-10-15 14:35:23.977CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Polar protein Wag31 both activates and inhibits cell wall metabolism at the poles and septum
title Polar protein Wag31 both activates and inhibits cell wall metabolism at the poles and septum
spellingShingle Polar protein Wag31 both activates and inhibits cell wall metabolism at the poles and septum
Habibi Arejan, Neda
ACCASE COMPLEX
DIVIVA, INTRACELLULAR MEMBRANE DOMAIN (IMD)
FTSI
MURG
MYCOBACTERIUM
PETIDOGLYCAN SYNTHESIS
POLAR GROWTH REGULATION
title_short Polar protein Wag31 both activates and inhibits cell wall metabolism at the poles and septum
title_full Polar protein Wag31 both activates and inhibits cell wall metabolism at the poles and septum
title_fullStr Polar protein Wag31 both activates and inhibits cell wall metabolism at the poles and septum
title_full_unstemmed Polar protein Wag31 both activates and inhibits cell wall metabolism at the poles and septum
title_sort Polar protein Wag31 both activates and inhibits cell wall metabolism at the poles and septum
dc.creator.none.fl_str_mv Habibi Arejan, Neda
Ensinck, Delfina
Diacovich, Lautaro
Patel, Parthvi Bharatkumar
Quintanilla, Samantha Y.
Emami Saleh, Arash
Gramajo, Hugo Cesar
Boutte, Cara C.
author Habibi Arejan, Neda
author_facet Habibi Arejan, Neda
Ensinck, Delfina
Diacovich, Lautaro
Patel, Parthvi Bharatkumar
Quintanilla, Samantha Y.
Emami Saleh, Arash
Gramajo, Hugo Cesar
Boutte, Cara C.
author_role author
author2 Ensinck, Delfina
Diacovich, Lautaro
Patel, Parthvi Bharatkumar
Quintanilla, Samantha Y.
Emami Saleh, Arash
Gramajo, Hugo Cesar
Boutte, Cara C.
author2_role author
author
author
author
author
author
author
dc.subject.none.fl_str_mv ACCASE COMPLEX
DIVIVA, INTRACELLULAR MEMBRANE DOMAIN (IMD)
FTSI
MURG
MYCOBACTERIUM
PETIDOGLYCAN SYNTHESIS
POLAR GROWTH REGULATION
topic ACCASE COMPLEX
DIVIVA, INTRACELLULAR MEMBRANE DOMAIN (IMD)
FTSI
MURG
MYCOBACTERIUM
PETIDOGLYCAN SYNTHESIS
POLAR GROWTH REGULATION
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv Mycobacterial cell elongation occurs at the cell poles; however, it is not clear how cell wall insertion is restricted to the pole or how it is organized. Wag31 is a pole-localized cytoplasmic protein that is essential for polar growth, but its molecular function has not been described. In this study we used alanine scanning mutagenesis to identify Wag31 residues involved in cell morphogenesis. Our data show that Wag31 helps to control proper septation as well as new and old pole elongation. We have identified key amino acid residues involved in these essential functions. Enzyme assays revealed that Wag31 interacts with lipid metabolism by modulating acyl-CoA carboxylase (ACCase) activity. We show that Wag31 does not control polar growth by regulating the localization of cell wall precursor enzymes to the Intracellular Membrane Domain, and we also demonstrate that phosphorylation of Wag31 does not substantively regulate peptidoglycan metabolism. This work establishes new regulatory functions of Wag31 in the mycobacterial cell cycle and clarifies the need for new molecular models of Wag31 function.
Fil: Habibi Arejan, Neda. University of Texas; Estados Unidos
Fil: Ensinck, Delfina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Rosario. Instituto de Biología Molecular y Celular de Rosario. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Instituto de Biología Molecular y Celular de Rosario; Argentina
Fil: Diacovich, Lautaro. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Rosario. Instituto de Biología Molecular y Celular de Rosario. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Instituto de Biología Molecular y Celular de Rosario; Argentina
Fil: Patel, Parthvi Bharatkumar. University of Texas; Estados Unidos
Fil: Quintanilla, Samantha Y.. University of Texas; Estados Unidos
Fil: Emami Saleh, Arash. University of Texas; Estados Unidos
Fil: Gramajo, Hugo Cesar. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Rosario. Instituto de Biología Molecular y Celular de Rosario. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Instituto de Biología Molecular y Celular de Rosario; Argentina
Fil: Boutte, Cara C.. University of Texas; Estados Unidos
description Mycobacterial cell elongation occurs at the cell poles; however, it is not clear how cell wall insertion is restricted to the pole or how it is organized. Wag31 is a pole-localized cytoplasmic protein that is essential for polar growth, but its molecular function has not been described. In this study we used alanine scanning mutagenesis to identify Wag31 residues involved in cell morphogenesis. Our data show that Wag31 helps to control proper septation as well as new and old pole elongation. We have identified key amino acid residues involved in these essential functions. Enzyme assays revealed that Wag31 interacts with lipid metabolism by modulating acyl-CoA carboxylase (ACCase) activity. We show that Wag31 does not control polar growth by regulating the localization of cell wall precursor enzymes to the Intracellular Membrane Domain, and we also demonstrate that phosphorylation of Wag31 does not substantively regulate peptidoglycan metabolism. This work establishes new regulatory functions of Wag31 in the mycobacterial cell cycle and clarifies the need for new molecular models of Wag31 function.
publishDate 2023
dc.date.none.fl_str_mv 2023-01
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/233260
Habibi Arejan, Neda; Ensinck, Delfina; Diacovich, Lautaro; Patel, Parthvi Bharatkumar; Quintanilla, Samantha Y.; et al.; Polar protein Wag31 both activates and inhibits cell wall metabolism at the poles and septum; Frontiers Media; Frontiers in Microbiology; 13; 1-2023; 1-15
1664-302X
CONICET Digital
CONICET
url http://hdl.handle.net/11336/233260
identifier_str_mv Habibi Arejan, Neda; Ensinck, Delfina; Diacovich, Lautaro; Patel, Parthvi Bharatkumar; Quintanilla, Samantha Y.; et al.; Polar protein Wag31 both activates and inhibits cell wall metabolism at the poles and septum; Frontiers Media; Frontiers in Microbiology; 13; 1-2023; 1-15
1664-302X
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://www.frontiersin.org/articles/10.3389/fmicb.2022.1085918/full
info:eu-repo/semantics/altIdentifier/doi/10.3389/fmicb.2022.1085918
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Frontiers Media
publisher.none.fl_str_mv Frontiers Media
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.221938

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