Cultivation-independent screening revealed hot spots of IncP-1, IncP-7 and IncP-9 plasmid occurrence in different environmental habitats

Autores
Dealtry, Simone; Ding, Guo Chun; Weichelt, Viola; Dunon, Vincent; Andreas, Schlüter; Martini, María Carla; del Papa, Maria Florencia; Lagares, Antonio; Auton Amos, Gregory Charles; Wellington, Elizabeth Margaret Helen; Gaze, William Hugo; Sipkema, Detmer; Sjoiling, Sara; Springael, Dirk; Heuer, Holger; van Elsas, Jan Dirk; Thomas, Christopher; Smalla, Kornelia
Año de publicación
2014
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
IncP-1, IncP-7 and IncP-9 plasmids often carry genes encoding enzymes involved in the degradation of man-made and natural contaminants, thus contributing to bacterial survival in polluted environments. However, the lack of suitable molecular tools often limits the detection of these plasmids in the environment. In this study, PCR followed by Southern blot hybridization detected the presence of plasmid-specific sequences in total community (TC-) DNA or fosmid DNA from samples originating from different environments and geographic regions. A novel primer system targeting IncP-9 plasmids was developed and applied along with established primers for IncP-1 and IncP-7. Screening TC-DNA from biopurification systems (BPS) which are used on farms for the purification of pesticide-contaminated water revealed high abundances of IncP-1 plasmids belonging to different subgroups as well as IncP-7 and IncP-9. The novel IncP-9 primer-system targeting the rep gene of nine IncP-9 subgroups allowed the detection of a high diversity of IncP-9 plasmid specific sequences in environments with different sources of pollution. Thus polluted sites are ??hot spots?? of plasmids potentially carrying catabolic genes.
Fil: Dealtry, Simone. Julius Kühn-Institut; Alemania
Fil: Ding, Guo Chun. Julius Kühn-Institut; Alemania
Fil: Weichelt, Viola. Julius Kühn-Institut; Alemania
Fil: Dunon, Vincent. Division of Soil and Water Management, KU Leuven; Bélgica
Fil: Andreas, Schlüter. Universitat Bielefeld; Alemania
Fil: Martini, María Carla. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Departamento de Ciencias Biológicas. Instituto de Biotecnología y Biología Molecular; Argentina
Fil: del Papa, Maria Florencia. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Departamento de Ciencias Biológicas. Instituto de Biotecnología y Biología Molecular; Argentina
Fil: Lagares, Antonio. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Departamento de Ciencias Biológicas. Instituto de Biotecnología y Biología Molecular; Argentina
Fil: Auton Amos, Gregory Charles. University of Warwick; Reino Unido
Fil: Wellington, Elizabeth Margaret Helen. University of Warwick; Reino Unido
Fil: Gaze, William Hugo. University of Warwick; Reino Unido
Fil: Sipkema, Detmer. Wageningen University; Países Bajos
Fil: Sjoiling, Sara. Sodertorn University; Suecia
Fil: Springael, Dirk. Division of Soil and Water Management, KU Leuven; Bélgica
Fil: Heuer, Holger. Julius Kühn-Institut; Alemania
Fil: van Elsas, Jan Dirk. University of Groningen; Países Bajos
Fil: Thomas, Christopher. University of Birmingham; Reino Unido
Fil: Smalla, Kornelia. Julius Kühn-Institut; Alemania
Materia
PLASMID
ENVIRONMENT
INCOMPATIBILITY GROUPS
POLLUTION
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/123601
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/123601
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Cultivation-independent screening revealed hot spots of IncP-1, IncP-7 and IncP-9 plasmid occurrence in different environmental habitatsDealtry, SimoneDing, Guo ChunWeichelt, ViolaDunon, VincentAndreas, SchlüterMartini, María Carladel Papa, Maria FlorenciaLagares, AntonioAuton Amos, Gregory CharlesWellington, Elizabeth Margaret HelenGaze, William HugoSipkema, DetmerSjoiling, SaraSpringael, DirkHeuer, Holgervan Elsas, Jan DirkThomas, ChristopherSmalla, KorneliaPLASMIDENVIRONMENTINCOMPATIBILITY GROUPSPOLLUTIONhttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1IncP-1, IncP-7 and IncP-9 plasmids often carry genes encoding enzymes involved in the degradation of man-made and natural contaminants, thus contributing to bacterial survival in polluted environments. However, the lack of suitable molecular tools often limits the detection of these plasmids in the environment. In this study, PCR followed by Southern blot hybridization detected the presence of plasmid-specific sequences in total community (TC-) DNA or fosmid DNA from samples originating from different environments and geographic regions. A novel primer system targeting IncP-9 plasmids was developed and applied along with established primers for IncP-1 and IncP-7. Screening TC-DNA from biopurification systems (BPS) which are used on farms for the purification of pesticide-contaminated water revealed high abundances of IncP-1 plasmids belonging to different subgroups as well as IncP-7 and IncP-9. The novel IncP-9 primer-system targeting the rep gene of nine IncP-9 subgroups allowed the detection of a high diversity of IncP-9 plasmid specific sequences in environments with different sources of pollution. Thus polluted sites are ??hot spots?? of plasmids potentially carrying catabolic genes.Fil: Dealtry, Simone. Julius Kühn-Institut; AlemaniaFil: Ding, Guo Chun. Julius Kühn-Institut; AlemaniaFil: Weichelt, Viola. Julius Kühn-Institut; AlemaniaFil: Dunon, Vincent. Division of Soil and Water Management, KU Leuven; BélgicaFil: Andreas, Schlüter. Universitat Bielefeld; AlemaniaFil: Martini, María Carla. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Departamento de Ciencias Biológicas. Instituto de Biotecnología y Biología Molecular; ArgentinaFil: del Papa, Maria Florencia. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Departamento de Ciencias Biológicas. Instituto de Biotecnología y Biología Molecular; ArgentinaFil: Lagares, Antonio. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Departamento de Ciencias Biológicas. Instituto de Biotecnología y Biología Molecular; ArgentinaFil: Auton Amos, Gregory Charles. University of Warwick; Reino UnidoFil: Wellington, Elizabeth Margaret Helen. University of Warwick; Reino UnidoFil: Gaze, William Hugo. University of Warwick; Reino UnidoFil: Sipkema, Detmer. Wageningen University; Países BajosFil: Sjoiling, Sara. Sodertorn University; SueciaFil: Springael, Dirk. Division of Soil and Water Management, KU Leuven; BélgicaFil: Heuer, Holger. Julius Kühn-Institut; AlemaniaFil: van Elsas, Jan Dirk. University of Groningen; Países BajosFil: Thomas, Christopher. University of Birmingham; Reino UnidoFil: Smalla, Kornelia. Julius Kühn-Institut; AlemaniaPublic Library of Science2014-02info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/123601Dealtry, Simone; Ding, Guo Chun; Weichelt, Viola; Dunon, Vincent; Andreas, Schlüter; et al.; Cultivation-independent screening revealed hot spots of IncP-1, IncP-7 and IncP-9 plasmid occurrence in different environmental habitats; Public Library of Science; Plos One; 9; 2-2014; 9-151932-6203CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/http://www.plosone.org/article/fetchObject.action?uri=info%3Adoi%2F10.1371%2Fjournal.pone.0089922&representation=PDFinfo:eu-repo/semantics/altIdentifier/doi/10.1371/journal.pone.0089922info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T09:56:00Zoai:ri.conicet.gov.ar:11336/123601instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 09:56:00.9CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Cultivation-independent screening revealed hot spots of IncP-1, IncP-7 and IncP-9 plasmid occurrence in different environmental habitats
title Cultivation-independent screening revealed hot spots of IncP-1, IncP-7 and IncP-9 plasmid occurrence in different environmental habitats
spellingShingle Cultivation-independent screening revealed hot spots of IncP-1, IncP-7 and IncP-9 plasmid occurrence in different environmental habitats
Dealtry, Simone
PLASMID
ENVIRONMENT
INCOMPATIBILITY GROUPS
POLLUTION
title_short Cultivation-independent screening revealed hot spots of IncP-1, IncP-7 and IncP-9 plasmid occurrence in different environmental habitats
title_full Cultivation-independent screening revealed hot spots of IncP-1, IncP-7 and IncP-9 plasmid occurrence in different environmental habitats
title_fullStr Cultivation-independent screening revealed hot spots of IncP-1, IncP-7 and IncP-9 plasmid occurrence in different environmental habitats
title_full_unstemmed Cultivation-independent screening revealed hot spots of IncP-1, IncP-7 and IncP-9 plasmid occurrence in different environmental habitats
title_sort Cultivation-independent screening revealed hot spots of IncP-1, IncP-7 and IncP-9 plasmid occurrence in different environmental habitats
dc.creator.none.fl_str_mv Dealtry, Simone
Ding, Guo Chun
Weichelt, Viola
Dunon, Vincent
Andreas, Schlüter
Martini, María Carla
del Papa, Maria Florencia
Lagares, Antonio
Auton Amos, Gregory Charles
Wellington, Elizabeth Margaret Helen
Gaze, William Hugo
Sipkema, Detmer
Sjoiling, Sara
Springael, Dirk
Heuer, Holger
van Elsas, Jan Dirk
Thomas, Christopher
Smalla, Kornelia
author Dealtry, Simone
author_facet Dealtry, Simone
Ding, Guo Chun
Weichelt, Viola
Dunon, Vincent
Andreas, Schlüter
Martini, María Carla
del Papa, Maria Florencia
Lagares, Antonio
Auton Amos, Gregory Charles
Wellington, Elizabeth Margaret Helen
Gaze, William Hugo
Sipkema, Detmer
Sjoiling, Sara
Springael, Dirk
Heuer, Holger
van Elsas, Jan Dirk
Thomas, Christopher
Smalla, Kornelia
author_role author
author2 Ding, Guo Chun
Weichelt, Viola
Dunon, Vincent
Andreas, Schlüter
Martini, María Carla
del Papa, Maria Florencia
Lagares, Antonio
Auton Amos, Gregory Charles
Wellington, Elizabeth Margaret Helen
Gaze, William Hugo
Sipkema, Detmer
Sjoiling, Sara
Springael, Dirk
Heuer, Holger
van Elsas, Jan Dirk
Thomas, Christopher
Smalla, Kornelia
author2_role author
author
author
author
author
author
author
author
author
author
author
author
author
author
author
author
author
dc.subject.none.fl_str_mv PLASMID
ENVIRONMENT
INCOMPATIBILITY GROUPS
POLLUTION
topic PLASMID
ENVIRONMENT
INCOMPATIBILITY GROUPS
POLLUTION
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv IncP-1, IncP-7 and IncP-9 plasmids often carry genes encoding enzymes involved in the degradation of man-made and natural contaminants, thus contributing to bacterial survival in polluted environments. However, the lack of suitable molecular tools often limits the detection of these plasmids in the environment. In this study, PCR followed by Southern blot hybridization detected the presence of plasmid-specific sequences in total community (TC-) DNA or fosmid DNA from samples originating from different environments and geographic regions. A novel primer system targeting IncP-9 plasmids was developed and applied along with established primers for IncP-1 and IncP-7. Screening TC-DNA from biopurification systems (BPS) which are used on farms for the purification of pesticide-contaminated water revealed high abundances of IncP-1 plasmids belonging to different subgroups as well as IncP-7 and IncP-9. The novel IncP-9 primer-system targeting the rep gene of nine IncP-9 subgroups allowed the detection of a high diversity of IncP-9 plasmid specific sequences in environments with different sources of pollution. Thus polluted sites are ??hot spots?? of plasmids potentially carrying catabolic genes.
Fil: Dealtry, Simone. Julius Kühn-Institut; Alemania
Fil: Ding, Guo Chun. Julius Kühn-Institut; Alemania
Fil: Weichelt, Viola. Julius Kühn-Institut; Alemania
Fil: Dunon, Vincent. Division of Soil and Water Management, KU Leuven; Bélgica
Fil: Andreas, Schlüter. Universitat Bielefeld; Alemania
Fil: Martini, María Carla. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Departamento de Ciencias Biológicas. Instituto de Biotecnología y Biología Molecular; Argentina
Fil: del Papa, Maria Florencia. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Departamento de Ciencias Biológicas. Instituto de Biotecnología y Biología Molecular; Argentina
Fil: Lagares, Antonio. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Departamento de Ciencias Biológicas. Instituto de Biotecnología y Biología Molecular; Argentina
Fil: Auton Amos, Gregory Charles. University of Warwick; Reino Unido
Fil: Wellington, Elizabeth Margaret Helen. University of Warwick; Reino Unido
Fil: Gaze, William Hugo. University of Warwick; Reino Unido
Fil: Sipkema, Detmer. Wageningen University; Países Bajos
Fil: Sjoiling, Sara. Sodertorn University; Suecia
Fil: Springael, Dirk. Division of Soil and Water Management, KU Leuven; Bélgica
Fil: Heuer, Holger. Julius Kühn-Institut; Alemania
Fil: van Elsas, Jan Dirk. University of Groningen; Países Bajos
Fil: Thomas, Christopher. University of Birmingham; Reino Unido
Fil: Smalla, Kornelia. Julius Kühn-Institut; Alemania
description IncP-1, IncP-7 and IncP-9 plasmids often carry genes encoding enzymes involved in the degradation of man-made and natural contaminants, thus contributing to bacterial survival in polluted environments. However, the lack of suitable molecular tools often limits the detection of these plasmids in the environment. In this study, PCR followed by Southern blot hybridization detected the presence of plasmid-specific sequences in total community (TC-) DNA or fosmid DNA from samples originating from different environments and geographic regions. A novel primer system targeting IncP-9 plasmids was developed and applied along with established primers for IncP-1 and IncP-7. Screening TC-DNA from biopurification systems (BPS) which are used on farms for the purification of pesticide-contaminated water revealed high abundances of IncP-1 plasmids belonging to different subgroups as well as IncP-7 and IncP-9. The novel IncP-9 primer-system targeting the rep gene of nine IncP-9 subgroups allowed the detection of a high diversity of IncP-9 plasmid specific sequences in environments with different sources of pollution. Thus polluted sites are ??hot spots?? of plasmids potentially carrying catabolic genes.
publishDate 2014
dc.date.none.fl_str_mv 2014-02
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/123601
Dealtry, Simone; Ding, Guo Chun; Weichelt, Viola; Dunon, Vincent; Andreas, Schlüter; et al.; Cultivation-independent screening revealed hot spots of IncP-1, IncP-7 and IncP-9 plasmid occurrence in different environmental habitats; Public Library of Science; Plos One; 9; 2-2014; 9-15
1932-6203
CONICET Digital
CONICET
url http://hdl.handle.net/11336/123601
identifier_str_mv Dealtry, Simone; Ding, Guo Chun; Weichelt, Viola; Dunon, Vincent; Andreas, Schlüter; et al.; Cultivation-independent screening revealed hot spots of IncP-1, IncP-7 and IncP-9 plasmid occurrence in different environmental habitats; Public Library of Science; Plos One; 9; 2-2014; 9-15
1932-6203
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/http://www.plosone.org/article/fetchObject.action?uri=info%3Adoi%2F10.1371%2Fjournal.pone.0089922&representation=PDF
info:eu-repo/semantics/altIdentifier/doi/10.1371/journal.pone.0089922
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Public Library of Science
publisher.none.fl_str_mv Public Library of Science
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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