Cultivation-independent screening revealed hot spots of IncP-1, IncP-7 and IncP-9 plasmid occurrence in different environmental habitats
- Autores
- Dealtry, Simone; Ding, Guo Chun; Weichelt, Viola; Dunon, Vincent; Andreas, Schlüter; Martini, María Carla; del Papa, Maria Florencia; Lagares, Antonio; Auton Amos, Gregory Charles; Wellington, Elizabeth Margaret Helen; Gaze, William Hugo; Sipkema, Detmer; Sjoiling, Sara; Springael, Dirk; Heuer, Holger; van Elsas, Jan Dirk; Thomas, Christopher; Smalla, Kornelia
- Año de publicación
- 2014
- Idioma
- inglés
- Tipo de recurso
- artículo
- Estado
- versión publicada
- Descripción
- IncP-1, IncP-7 and IncP-9 plasmids often carry genes encoding enzymes involved in the degradation of man-made and natural contaminants, thus contributing to bacterial survival in polluted environments. However, the lack of suitable molecular tools often limits the detection of these plasmids in the environment. In this study, PCR followed by Southern blot hybridization detected the presence of plasmid-specific sequences in total community (TC-) DNA or fosmid DNA from samples originating from different environments and geographic regions. A novel primer system targeting IncP-9 plasmids was developed and applied along with established primers for IncP-1 and IncP-7. Screening TC-DNA from biopurification systems (BPS) which are used on farms for the purification of pesticide-contaminated water revealed high abundances of IncP-1 plasmids belonging to different subgroups as well as IncP-7 and IncP-9. The novel IncP-9 primer-system targeting the rep gene of nine IncP-9 subgroups allowed the detection of a high diversity of IncP-9 plasmid specific sequences in environments with different sources of pollution. Thus polluted sites are ??hot spots?? of plasmids potentially carrying catabolic genes.
Fil: Dealtry, Simone. Julius Kühn-Institut; Alemania
Fil: Ding, Guo Chun. Julius Kühn-Institut; Alemania
Fil: Weichelt, Viola. Julius Kühn-Institut; Alemania
Fil: Dunon, Vincent. Division of Soil and Water Management, KU Leuven; Bélgica
Fil: Andreas, Schlüter. Universitat Bielefeld; Alemania
Fil: Martini, María Carla. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Departamento de Ciencias Biológicas. Instituto de Biotecnología y Biología Molecular; Argentina
Fil: del Papa, Maria Florencia. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Departamento de Ciencias Biológicas. Instituto de Biotecnología y Biología Molecular; Argentina
Fil: Lagares, Antonio. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Departamento de Ciencias Biológicas. Instituto de Biotecnología y Biología Molecular; Argentina
Fil: Auton Amos, Gregory Charles. University of Warwick; Reino Unido
Fil: Wellington, Elizabeth Margaret Helen. University of Warwick; Reino Unido
Fil: Gaze, William Hugo. University of Warwick; Reino Unido
Fil: Sipkema, Detmer. Wageningen University; Países Bajos
Fil: Sjoiling, Sara. Sodertorn University; Suecia
Fil: Springael, Dirk. Division of Soil and Water Management, KU Leuven; Bélgica
Fil: Heuer, Holger. Julius Kühn-Institut; Alemania
Fil: van Elsas, Jan Dirk. University of Groningen; Países Bajos
Fil: Thomas, Christopher. University of Birmingham; Reino Unido
Fil: Smalla, Kornelia. Julius Kühn-Institut; Alemania - Materia
-
PLASMID
ENVIRONMENT
INCOMPATIBILITY GROUPS
POLLUTION - Nivel de accesibilidad
- acceso abierto
- Condiciones de uso
- https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
- Repositorio
.jpg)
- Institución
- Consejo Nacional de Investigaciones Científicas y Técnicas
- OAI Identificador
- oai:ri.conicet.gov.ar:11336/123601
Ver los metadatos del registro completo
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Cultivation-independent screening revealed hot spots of IncP-1, IncP-7 and IncP-9 plasmid occurrence in different environmental habitatsDealtry, SimoneDing, Guo ChunWeichelt, ViolaDunon, VincentAndreas, SchlüterMartini, María Carladel Papa, Maria FlorenciaLagares, AntonioAuton Amos, Gregory CharlesWellington, Elizabeth Margaret HelenGaze, William HugoSipkema, DetmerSjoiling, SaraSpringael, DirkHeuer, Holgervan Elsas, Jan DirkThomas, ChristopherSmalla, KorneliaPLASMIDENVIRONMENTINCOMPATIBILITY GROUPSPOLLUTIONhttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1IncP-1, IncP-7 and IncP-9 plasmids often carry genes encoding enzymes involved in the degradation of man-made and natural contaminants, thus contributing to bacterial survival in polluted environments. However, the lack of suitable molecular tools often limits the detection of these plasmids in the environment. In this study, PCR followed by Southern blot hybridization detected the presence of plasmid-specific sequences in total community (TC-) DNA or fosmid DNA from samples originating from different environments and geographic regions. A novel primer system targeting IncP-9 plasmids was developed and applied along with established primers for IncP-1 and IncP-7. Screening TC-DNA from biopurification systems (BPS) which are used on farms for the purification of pesticide-contaminated water revealed high abundances of IncP-1 plasmids belonging to different subgroups as well as IncP-7 and IncP-9. The novel IncP-9 primer-system targeting the rep gene of nine IncP-9 subgroups allowed the detection of a high diversity of IncP-9 plasmid specific sequences in environments with different sources of pollution. Thus polluted sites are ??hot spots?? of plasmids potentially carrying catabolic genes.Fil: Dealtry, Simone. Julius Kühn-Institut; AlemaniaFil: Ding, Guo Chun. Julius Kühn-Institut; AlemaniaFil: Weichelt, Viola. Julius Kühn-Institut; AlemaniaFil: Dunon, Vincent. Division of Soil and Water Management, KU Leuven; BélgicaFil: Andreas, Schlüter. Universitat Bielefeld; AlemaniaFil: Martini, María Carla. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Departamento de Ciencias Biológicas. Instituto de Biotecnología y Biología Molecular; ArgentinaFil: del Papa, Maria Florencia. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Departamento de Ciencias Biológicas. Instituto de Biotecnología y Biología Molecular; ArgentinaFil: Lagares, Antonio. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Departamento de Ciencias Biológicas. Instituto de Biotecnología y Biología Molecular; ArgentinaFil: Auton Amos, Gregory Charles. University of Warwick; Reino UnidoFil: Wellington, Elizabeth Margaret Helen. University of Warwick; Reino UnidoFil: Gaze, William Hugo. University of Warwick; Reino UnidoFil: Sipkema, Detmer. Wageningen University; Países BajosFil: Sjoiling, Sara. Sodertorn University; SueciaFil: Springael, Dirk. Division of Soil and Water Management, KU Leuven; BélgicaFil: Heuer, Holger. Julius Kühn-Institut; AlemaniaFil: van Elsas, Jan Dirk. University of Groningen; Países BajosFil: Thomas, Christopher. University of Birmingham; Reino UnidoFil: Smalla, Kornelia. Julius Kühn-Institut; AlemaniaPublic Library of Science2014-02info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/123601Dealtry, Simone; Ding, Guo Chun; Weichelt, Viola; Dunon, Vincent; Andreas, Schlüter; et al.; Cultivation-independent screening revealed hot spots of IncP-1, IncP-7 and IncP-9 plasmid occurrence in different environmental habitats; Public Library of Science; Plos One; 9; 2-2014; 9-151932-6203CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/http://www.plosone.org/article/fetchObject.action?uri=info%3Adoi%2F10.1371%2Fjournal.pone.0089922&representation=PDFinfo:eu-repo/semantics/altIdentifier/doi/10.1371/journal.pone.0089922info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-10-22T11:25:09Zoai:ri.conicet.gov.ar:11336/123601instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-10-22 11:25:10.166CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse |
| dc.title.none.fl_str_mv |
Cultivation-independent screening revealed hot spots of IncP-1, IncP-7 and IncP-9 plasmid occurrence in different environmental habitats |
| title |
Cultivation-independent screening revealed hot spots of IncP-1, IncP-7 and IncP-9 plasmid occurrence in different environmental habitats |
| spellingShingle |
Cultivation-independent screening revealed hot spots of IncP-1, IncP-7 and IncP-9 plasmid occurrence in different environmental habitats Dealtry, Simone PLASMID ENVIRONMENT INCOMPATIBILITY GROUPS POLLUTION |
| title_short |
Cultivation-independent screening revealed hot spots of IncP-1, IncP-7 and IncP-9 plasmid occurrence in different environmental habitats |
| title_full |
Cultivation-independent screening revealed hot spots of IncP-1, IncP-7 and IncP-9 plasmid occurrence in different environmental habitats |
| title_fullStr |
Cultivation-independent screening revealed hot spots of IncP-1, IncP-7 and IncP-9 plasmid occurrence in different environmental habitats |
| title_full_unstemmed |
Cultivation-independent screening revealed hot spots of IncP-1, IncP-7 and IncP-9 plasmid occurrence in different environmental habitats |
| title_sort |
Cultivation-independent screening revealed hot spots of IncP-1, IncP-7 and IncP-9 plasmid occurrence in different environmental habitats |
| dc.creator.none.fl_str_mv |
Dealtry, Simone Ding, Guo Chun Weichelt, Viola Dunon, Vincent Andreas, Schlüter Martini, María Carla del Papa, Maria Florencia Lagares, Antonio Auton Amos, Gregory Charles Wellington, Elizabeth Margaret Helen Gaze, William Hugo Sipkema, Detmer Sjoiling, Sara Springael, Dirk Heuer, Holger van Elsas, Jan Dirk Thomas, Christopher Smalla, Kornelia |
| author |
Dealtry, Simone |
| author_facet |
Dealtry, Simone Ding, Guo Chun Weichelt, Viola Dunon, Vincent Andreas, Schlüter Martini, María Carla del Papa, Maria Florencia Lagares, Antonio Auton Amos, Gregory Charles Wellington, Elizabeth Margaret Helen Gaze, William Hugo Sipkema, Detmer Sjoiling, Sara Springael, Dirk Heuer, Holger van Elsas, Jan Dirk Thomas, Christopher Smalla, Kornelia |
| author_role |
author |
| author2 |
Ding, Guo Chun Weichelt, Viola Dunon, Vincent Andreas, Schlüter Martini, María Carla del Papa, Maria Florencia Lagares, Antonio Auton Amos, Gregory Charles Wellington, Elizabeth Margaret Helen Gaze, William Hugo Sipkema, Detmer Sjoiling, Sara Springael, Dirk Heuer, Holger van Elsas, Jan Dirk Thomas, Christopher Smalla, Kornelia |
| author2_role |
author author author author author author author author author author author author author author author author author |
| dc.subject.none.fl_str_mv |
PLASMID ENVIRONMENT INCOMPATIBILITY GROUPS POLLUTION |
| topic |
PLASMID ENVIRONMENT INCOMPATIBILITY GROUPS POLLUTION |
| purl_subject.fl_str_mv |
https://purl.org/becyt/ford/1.6 https://purl.org/becyt/ford/1 |
| dc.description.none.fl_txt_mv |
IncP-1, IncP-7 and IncP-9 plasmids often carry genes encoding enzymes involved in the degradation of man-made and natural contaminants, thus contributing to bacterial survival in polluted environments. However, the lack of suitable molecular tools often limits the detection of these plasmids in the environment. In this study, PCR followed by Southern blot hybridization detected the presence of plasmid-specific sequences in total community (TC-) DNA or fosmid DNA from samples originating from different environments and geographic regions. A novel primer system targeting IncP-9 plasmids was developed and applied along with established primers for IncP-1 and IncP-7. Screening TC-DNA from biopurification systems (BPS) which are used on farms for the purification of pesticide-contaminated water revealed high abundances of IncP-1 plasmids belonging to different subgroups as well as IncP-7 and IncP-9. The novel IncP-9 primer-system targeting the rep gene of nine IncP-9 subgroups allowed the detection of a high diversity of IncP-9 plasmid specific sequences in environments with different sources of pollution. Thus polluted sites are ??hot spots?? of plasmids potentially carrying catabolic genes. Fil: Dealtry, Simone. Julius Kühn-Institut; Alemania Fil: Ding, Guo Chun. Julius Kühn-Institut; Alemania Fil: Weichelt, Viola. Julius Kühn-Institut; Alemania Fil: Dunon, Vincent. Division of Soil and Water Management, KU Leuven; Bélgica Fil: Andreas, Schlüter. Universitat Bielefeld; Alemania Fil: Martini, María Carla. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Departamento de Ciencias Biológicas. Instituto de Biotecnología y Biología Molecular; Argentina Fil: del Papa, Maria Florencia. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Departamento de Ciencias Biológicas. Instituto de Biotecnología y Biología Molecular; Argentina Fil: Lagares, Antonio. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Departamento de Ciencias Biológicas. Instituto de Biotecnología y Biología Molecular; Argentina Fil: Auton Amos, Gregory Charles. University of Warwick; Reino Unido Fil: Wellington, Elizabeth Margaret Helen. University of Warwick; Reino Unido Fil: Gaze, William Hugo. University of Warwick; Reino Unido Fil: Sipkema, Detmer. Wageningen University; Países Bajos Fil: Sjoiling, Sara. Sodertorn University; Suecia Fil: Springael, Dirk. Division of Soil and Water Management, KU Leuven; Bélgica Fil: Heuer, Holger. Julius Kühn-Institut; Alemania Fil: van Elsas, Jan Dirk. University of Groningen; Países Bajos Fil: Thomas, Christopher. University of Birmingham; Reino Unido Fil: Smalla, Kornelia. Julius Kühn-Institut; Alemania |
| description |
IncP-1, IncP-7 and IncP-9 plasmids often carry genes encoding enzymes involved in the degradation of man-made and natural contaminants, thus contributing to bacterial survival in polluted environments. However, the lack of suitable molecular tools often limits the detection of these plasmids in the environment. In this study, PCR followed by Southern blot hybridization detected the presence of plasmid-specific sequences in total community (TC-) DNA or fosmid DNA from samples originating from different environments and geographic regions. A novel primer system targeting IncP-9 plasmids was developed and applied along with established primers for IncP-1 and IncP-7. Screening TC-DNA from biopurification systems (BPS) which are used on farms for the purification of pesticide-contaminated water revealed high abundances of IncP-1 plasmids belonging to different subgroups as well as IncP-7 and IncP-9. The novel IncP-9 primer-system targeting the rep gene of nine IncP-9 subgroups allowed the detection of a high diversity of IncP-9 plasmid specific sequences in environments with different sources of pollution. Thus polluted sites are ??hot spots?? of plasmids potentially carrying catabolic genes. |
| publishDate |
2014 |
| dc.date.none.fl_str_mv |
2014-02 |
| dc.type.none.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion http://purl.org/coar/resource_type/c_6501 info:ar-repo/semantics/articulo |
| format |
article |
| status_str |
publishedVersion |
| dc.identifier.none.fl_str_mv |
http://hdl.handle.net/11336/123601 Dealtry, Simone; Ding, Guo Chun; Weichelt, Viola; Dunon, Vincent; Andreas, Schlüter; et al.; Cultivation-independent screening revealed hot spots of IncP-1, IncP-7 and IncP-9 plasmid occurrence in different environmental habitats; Public Library of Science; Plos One; 9; 2-2014; 9-15 1932-6203 CONICET Digital CONICET |
| url |
http://hdl.handle.net/11336/123601 |
| identifier_str_mv |
Dealtry, Simone; Ding, Guo Chun; Weichelt, Viola; Dunon, Vincent; Andreas, Schlüter; et al.; Cultivation-independent screening revealed hot spots of IncP-1, IncP-7 and IncP-9 plasmid occurrence in different environmental habitats; Public Library of Science; Plos One; 9; 2-2014; 9-15 1932-6203 CONICET Digital CONICET |
| dc.language.none.fl_str_mv |
eng |
| language |
eng |
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Public Library of Science |
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CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas |
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