Enzymatic Potential of Schizophyllum commune BNT39 in BHET Hydrolysis and PET Biodegradation

Autores
Martínez, Fernando Gabriel; Canal Martinez, Veronica de Los Angeles; Pereira, Claudia Elizabeth; Zannier, Federico; Arnau, Victor Gonzalo; Romero, Cintia Mariana; Alvarez, Analia
Año de publicación
2025
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
The accumulation of polyethylene terephthalate (PET) in the environment demands efficientmicrobial strategies for its degradation. This study evaluates the biodegradation potentialof Schizophyllum commune BNT39 toward bis(2-hydroxyethyl) terephthalate (BHET),a major PET intermediate, and PET itself. Clear halos on BHET-agar plates indicatedextracellular hydrolytic activity. In liquid culture, thin-layer chromatography (TLC) andhigh-performance liquid chromatography (HPLC) analyses revealed a three-phase degradationprofile characterized by rapid BHET hydrolysis, transient dimer accumulation, andsubsequent conversion to terephthalic acid (TPA). BHET was reduced by approximately96% within seven days, while TPA accumulation reached 0.8 mg/mL after 30 days ofincubation. Although PET degradation was limited, TPA was consistently detected as theprincipal product, with no BHET or MHET intermediates. To explore strategies for enhancingenzymatic activity, apple-derived cutin, PET, BHET, and polycaprolactone (PCL) weretested as inducers. Cutin markedly stimulated extracellular enzyme production, suggestingactivation of cutinase-like enzymes. Overall, S. commune BNT39 demonstrates the ability totransform PET-related substrates, with cutin emerging as a promising natural stimulant toenhance enzymatic depolymerization. Future studies should focus on enzyme purification,activity profiling, and reaction optimization near PET’s glass transition temperature, wherethe polymer becomes more accessible for enzymatic attack.
Fil: Martínez, Fernando Gabriel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Planta Piloto de Procesos Industriales Microbiológicos; Argentina
Fil: Canal Martinez, Veronica de Los Angeles. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Planta Piloto de Procesos Industriales Microbiológicos; Argentina
Fil: Pereira, Claudia Elizabeth. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Planta Piloto de Procesos Industriales Microbiológicos; Argentina
Fil: Zannier, Federico. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Planta Piloto de Procesos Industriales Microbiológicos; Argentina
Fil: Arnau, Victor Gonzalo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Planta Piloto de Procesos Industriales Microbiológicos; Argentina
Fil: Romero, Cintia Mariana. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Planta Piloto de Procesos Industriales Microbiológicos; Argentina. Universidad Nacional de Tucumán. Facultad de Bioquímica, Química y Farmacia; Argentina
Fil: Alvarez, Analia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Planta Piloto de Procesos Industriales Microbiológicos; Argentina. Universidad Nacional de Tucumán. Facultad de Ciencias Naturales e Instituto Miguel Lillo; Argentina
Materia
Plastic Biodegradation
PET Intermediates
Fungal Cutinase
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/277747
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/277747
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Enzymatic Potential of Schizophyllum commune BNT39 in BHET Hydrolysis and PET BiodegradationMartínez, Fernando GabrielCanal Martinez, Veronica de Los AngelesPereira, Claudia ElizabethZannier, FedericoArnau, Victor GonzaloRomero, Cintia MarianaAlvarez, AnaliaPlastic BiodegradationPET IntermediatesFungal Cutinasehttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1The accumulation of polyethylene terephthalate (PET) in the environment demands efficientmicrobial strategies for its degradation. This study evaluates the biodegradation potentialof Schizophyllum commune BNT39 toward bis(2-hydroxyethyl) terephthalate (BHET),a major PET intermediate, and PET itself. Clear halos on BHET-agar plates indicatedextracellular hydrolytic activity. In liquid culture, thin-layer chromatography (TLC) andhigh-performance liquid chromatography (HPLC) analyses revealed a three-phase degradationprofile characterized by rapid BHET hydrolysis, transient dimer accumulation, andsubsequent conversion to terephthalic acid (TPA). BHET was reduced by approximately96% within seven days, while TPA accumulation reached 0.8 mg/mL after 30 days ofincubation. Although PET degradation was limited, TPA was consistently detected as theprincipal product, with no BHET or MHET intermediates. To explore strategies for enhancingenzymatic activity, apple-derived cutin, PET, BHET, and polycaprolactone (PCL) weretested as inducers. Cutin markedly stimulated extracellular enzyme production, suggestingactivation of cutinase-like enzymes. Overall, S. commune BNT39 demonstrates the ability totransform PET-related substrates, with cutin emerging as a promising natural stimulant toenhance enzymatic depolymerization. Future studies should focus on enzyme purification,activity profiling, and reaction optimization near PET’s glass transition temperature, wherethe polymer becomes more accessible for enzymatic attack.Fil: Martínez, Fernando Gabriel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Planta Piloto de Procesos Industriales Microbiológicos; ArgentinaFil: Canal Martinez, Veronica de Los Angeles. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Planta Piloto de Procesos Industriales Microbiológicos; ArgentinaFil: Pereira, Claudia Elizabeth. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Planta Piloto de Procesos Industriales Microbiológicos; ArgentinaFil: Zannier, Federico. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Planta Piloto de Procesos Industriales Microbiológicos; ArgentinaFil: Arnau, Victor Gonzalo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Planta Piloto de Procesos Industriales Microbiológicos; ArgentinaFil: Romero, Cintia Mariana. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Planta Piloto de Procesos Industriales Microbiológicos; Argentina. Universidad Nacional de Tucumán. Facultad de Bioquímica, Química y Farmacia; ArgentinaFil: Alvarez, Analia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Planta Piloto de Procesos Industriales Microbiológicos; Argentina. Universidad Nacional de Tucumán. Facultad de Ciencias Naturales e Instituto Miguel Lillo; ArgentinaMultidisciplinary Digital Publishing Institute2025-11info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/277747Martínez, Fernando Gabriel; Canal Martinez, Veronica de Los Angeles; Pereira, Claudia Elizabeth; Zannier, Federico; Arnau, Victor Gonzalo; et al.; Enzymatic Potential of Schizophyllum commune BNT39 in BHET Hydrolysis and PET Biodegradation; Multidisciplinary Digital Publishing Institute; Processes; 13; 11; 11-2025; 1-172227-9717CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://www.mdpi.com/2227-9717/13/11/3663info:eu-repo/semantics/altIdentifier/doi/10.3390/pr13113663info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-12-23T14:54:26Zoai:ri.conicet.gov.ar:11336/277747instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-12-23 14:54:27.158CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Enzymatic Potential of Schizophyllum commune BNT39 in BHET Hydrolysis and PET Biodegradation
title Enzymatic Potential of Schizophyllum commune BNT39 in BHET Hydrolysis and PET Biodegradation
spellingShingle Enzymatic Potential of Schizophyllum commune BNT39 in BHET Hydrolysis and PET Biodegradation
Martínez, Fernando Gabriel
Plastic Biodegradation
PET Intermediates
Fungal Cutinase
title_short Enzymatic Potential of Schizophyllum commune BNT39 in BHET Hydrolysis and PET Biodegradation
title_full Enzymatic Potential of Schizophyllum commune BNT39 in BHET Hydrolysis and PET Biodegradation
title_fullStr Enzymatic Potential of Schizophyllum commune BNT39 in BHET Hydrolysis and PET Biodegradation
title_full_unstemmed Enzymatic Potential of Schizophyllum commune BNT39 in BHET Hydrolysis and PET Biodegradation
title_sort Enzymatic Potential of Schizophyllum commune BNT39 in BHET Hydrolysis and PET Biodegradation
dc.creator.none.fl_str_mv Martínez, Fernando Gabriel
Canal Martinez, Veronica de Los Angeles
Pereira, Claudia Elizabeth
Zannier, Federico
Arnau, Victor Gonzalo
Romero, Cintia Mariana
Alvarez, Analia
author Martínez, Fernando Gabriel
author_facet Martínez, Fernando Gabriel
Canal Martinez, Veronica de Los Angeles
Pereira, Claudia Elizabeth
Zannier, Federico
Arnau, Victor Gonzalo
Romero, Cintia Mariana
Alvarez, Analia
author_role author
author2 Canal Martinez, Veronica de Los Angeles
Pereira, Claudia Elizabeth
Zannier, Federico
Arnau, Victor Gonzalo
Romero, Cintia Mariana
Alvarez, Analia
author2_role author
author
author
author
author
author
dc.subject.none.fl_str_mv Plastic Biodegradation
PET Intermediates
Fungal Cutinase
topic Plastic Biodegradation
PET Intermediates
Fungal Cutinase
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv The accumulation of polyethylene terephthalate (PET) in the environment demands efficientmicrobial strategies for its degradation. This study evaluates the biodegradation potentialof Schizophyllum commune BNT39 toward bis(2-hydroxyethyl) terephthalate (BHET),a major PET intermediate, and PET itself. Clear halos on BHET-agar plates indicatedextracellular hydrolytic activity. In liquid culture, thin-layer chromatography (TLC) andhigh-performance liquid chromatography (HPLC) analyses revealed a three-phase degradationprofile characterized by rapid BHET hydrolysis, transient dimer accumulation, andsubsequent conversion to terephthalic acid (TPA). BHET was reduced by approximately96% within seven days, while TPA accumulation reached 0.8 mg/mL after 30 days ofincubation. Although PET degradation was limited, TPA was consistently detected as theprincipal product, with no BHET or MHET intermediates. To explore strategies for enhancingenzymatic activity, apple-derived cutin, PET, BHET, and polycaprolactone (PCL) weretested as inducers. Cutin markedly stimulated extracellular enzyme production, suggestingactivation of cutinase-like enzymes. Overall, S. commune BNT39 demonstrates the ability totransform PET-related substrates, with cutin emerging as a promising natural stimulant toenhance enzymatic depolymerization. Future studies should focus on enzyme purification,activity profiling, and reaction optimization near PET’s glass transition temperature, wherethe polymer becomes more accessible for enzymatic attack.
Fil: Martínez, Fernando Gabriel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Planta Piloto de Procesos Industriales Microbiológicos; Argentina
Fil: Canal Martinez, Veronica de Los Angeles. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Planta Piloto de Procesos Industriales Microbiológicos; Argentina
Fil: Pereira, Claudia Elizabeth. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Planta Piloto de Procesos Industriales Microbiológicos; Argentina
Fil: Zannier, Federico. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Planta Piloto de Procesos Industriales Microbiológicos; Argentina
Fil: Arnau, Victor Gonzalo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Planta Piloto de Procesos Industriales Microbiológicos; Argentina
Fil: Romero, Cintia Mariana. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Planta Piloto de Procesos Industriales Microbiológicos; Argentina. Universidad Nacional de Tucumán. Facultad de Bioquímica, Química y Farmacia; Argentina
Fil: Alvarez, Analia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Planta Piloto de Procesos Industriales Microbiológicos; Argentina. Universidad Nacional de Tucumán. Facultad de Ciencias Naturales e Instituto Miguel Lillo; Argentina
description The accumulation of polyethylene terephthalate (PET) in the environment demands efficientmicrobial strategies for its degradation. This study evaluates the biodegradation potentialof Schizophyllum commune BNT39 toward bis(2-hydroxyethyl) terephthalate (BHET),a major PET intermediate, and PET itself. Clear halos on BHET-agar plates indicatedextracellular hydrolytic activity. In liquid culture, thin-layer chromatography (TLC) andhigh-performance liquid chromatography (HPLC) analyses revealed a three-phase degradationprofile characterized by rapid BHET hydrolysis, transient dimer accumulation, andsubsequent conversion to terephthalic acid (TPA). BHET was reduced by approximately96% within seven days, while TPA accumulation reached 0.8 mg/mL after 30 days ofincubation. Although PET degradation was limited, TPA was consistently detected as theprincipal product, with no BHET or MHET intermediates. To explore strategies for enhancingenzymatic activity, apple-derived cutin, PET, BHET, and polycaprolactone (PCL) weretested as inducers. Cutin markedly stimulated extracellular enzyme production, suggestingactivation of cutinase-like enzymes. Overall, S. commune BNT39 demonstrates the ability totransform PET-related substrates, with cutin emerging as a promising natural stimulant toenhance enzymatic depolymerization. Future studies should focus on enzyme purification,activity profiling, and reaction optimization near PET’s glass transition temperature, wherethe polymer becomes more accessible for enzymatic attack.
publishDate 2025
dc.date.none.fl_str_mv 2025-11
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/277747
Martínez, Fernando Gabriel; Canal Martinez, Veronica de Los Angeles; Pereira, Claudia Elizabeth; Zannier, Federico; Arnau, Victor Gonzalo; et al.; Enzymatic Potential of Schizophyllum commune BNT39 in BHET Hydrolysis and PET Biodegradation; Multidisciplinary Digital Publishing Institute; Processes; 13; 11; 11-2025; 1-17
2227-9717
CONICET Digital
CONICET
url http://hdl.handle.net/11336/277747
identifier_str_mv Martínez, Fernando Gabriel; Canal Martinez, Veronica de Los Angeles; Pereira, Claudia Elizabeth; Zannier, Federico; Arnau, Victor Gonzalo; et al.; Enzymatic Potential of Schizophyllum commune BNT39 in BHET Hydrolysis and PET Biodegradation; Multidisciplinary Digital Publishing Institute; Processes; 13; 11; 11-2025; 1-17
2227-9717
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://www.mdpi.com/2227-9717/13/11/3663
info:eu-repo/semantics/altIdentifier/doi/10.3390/pr13113663
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Multidisciplinary Digital Publishing Institute
publisher.none.fl_str_mv Multidisciplinary Digital Publishing Institute
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 12.952241

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