Characterization of a modified gold platform for the development of a label-free anti-thrombin aptasensor

Autores
Jalit, Yamile; Gutierrez, Fabiana Andrea; Dubacheva, Galina; Goyer, Cedric; Coche Guerente, Liliane; Defrancq, Eric; Labbe, Pierre; Rivas, Gustavo Adolfo; Rodriguez, Marcela Cecilia
Año de publicación
2012
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
This work reports the characterization of a modified gold surface as a platform for the development of a label free aptasensor for thrombin detection. The biorecognition platform was obtained by the self-assembly of 4-mercaptobenzoic acid onto a gold surface, covalent attachment of streptavidin and further immobilization of the biotinylated anti-thrombin aptamer. The biosensing platform was characterized by cyclic voltammetry, electrochemical impedance spectroscopy, surface plasmon resonance (SPR) and quartz crystal microbalance with dissipation monitoring. The biorecognition event aptamer-thrombin was detected from changes in the SPR angle produced as a consequence of the molecular interaction between the aptasensor and the target protein. The biosensing platform demonstrated to be highly selective for human thrombin even in the presence of large excess of bovine thrombin, bovine serum albumin, cytochrome C, lysozyme and myoglobin. The relationship between the changes in the SPR angle and thrombin concentration was linear up to 0.19 μmol L−1 (R2=0.992) while the detection limit was of 12.0 nmol L−1 (240 fmol in the sample). This new sensing approach represents an interesting and promising alternative for the SPR-based quantification of thrombin.
Fil: Jalit, Yamile. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Investigaciones en Físico-química de Córdoba. Universidad Nacional de Córdoba. Facultad de Ciencias Químicas. Instituto de Investigaciones en Físico-química de Córdoba; Argentina
Fil: Gutierrez, Fabiana Andrea. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Investigaciones en Físico-química de Córdoba. Universidad Nacional de Córdoba. Facultad de Ciencias Químicas. Instituto de Investigaciones en Físico-química de Córdoba; Argentina
Fil: Dubacheva, Galina. Universite Joseph Fourier; Francia
Fil: Goyer, Cedric. Universite Joseph Fourier; Francia
Fil: Coche Guerente, Liliane. Universite Joseph Fourier; Francia
Fil: Defrancq, Eric. Universite Joseph Fourier; Francia
Fil: Labbe, Pierre. Universite Joseph Fourier; Francia
Fil: Rivas, Gustavo Adolfo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Investigaciones en Físico-química de Córdoba. Universidad Nacional de Córdoba. Facultad de Ciencias Químicas. Instituto de Investigaciones en Físico-química de Córdoba; Argentina
Fil: Rodriguez, Marcela Cecilia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Investigaciones en Físico-química de Córdoba. Universidad Nacional de Córdoba. Facultad de Ciencias Químicas. Instituto de Investigaciones en Físico-química de Córdoba; Argentina
Materia
Thrombin Binding Aptamer
Aptasensor
Optical Aptasensor
Thrombin Biosensor
Protein Detection
Spr Sensing
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/26000
Acceder
id CONICETDig_59b26ea3bb4063201102c27c3a5e0701
oai_identifier_str oai:ri.conicet.gov.ar:11336/26000
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Characterization of a modified gold platform for the development of a label-free anti-thrombin aptasensorJalit, YamileGutierrez, Fabiana AndreaDubacheva, GalinaGoyer, CedricCoche Guerente, LilianeDefrancq, EricLabbe, PierreRivas, Gustavo AdolfoRodriguez, Marcela CeciliaThrombin Binding AptamerAptasensorOptical AptasensorThrombin BiosensorProtein DetectionSpr Sensinghttps://purl.org/becyt/ford/1.4https://purl.org/becyt/ford/1This work reports the characterization of a modified gold surface as a platform for the development of a label free aptasensor for thrombin detection. The biorecognition platform was obtained by the self-assembly of 4-mercaptobenzoic acid onto a gold surface, covalent attachment of streptavidin and further immobilization of the biotinylated anti-thrombin aptamer. The biosensing platform was characterized by cyclic voltammetry, electrochemical impedance spectroscopy, surface plasmon resonance (SPR) and quartz crystal microbalance with dissipation monitoring. The biorecognition event aptamer-thrombin was detected from changes in the SPR angle produced as a consequence of the molecular interaction between the aptasensor and the target protein. The biosensing platform demonstrated to be highly selective for human thrombin even in the presence of large excess of bovine thrombin, bovine serum albumin, cytochrome C, lysozyme and myoglobin. The relationship between the changes in the SPR angle and thrombin concentration was linear up to 0.19 μmol L−1 (R2=0.992) while the detection limit was of 12.0 nmol L−1 (240 fmol in the sample). This new sensing approach represents an interesting and promising alternative for the SPR-based quantification of thrombin.Fil: Jalit, Yamile. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Investigaciones en Físico-química de Córdoba. Universidad Nacional de Córdoba. Facultad de Ciencias Químicas. Instituto de Investigaciones en Físico-química de Córdoba; ArgentinaFil: Gutierrez, Fabiana Andrea. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Investigaciones en Físico-química de Córdoba. Universidad Nacional de Córdoba. Facultad de Ciencias Químicas. Instituto de Investigaciones en Físico-química de Córdoba; ArgentinaFil: Dubacheva, Galina. Universite Joseph Fourier; FranciaFil: Goyer, Cedric. Universite Joseph Fourier; FranciaFil: Coche Guerente, Liliane. Universite Joseph Fourier; FranciaFil: Defrancq, Eric. Universite Joseph Fourier; FranciaFil: Labbe, Pierre. Universite Joseph Fourier; FranciaFil: Rivas, Gustavo Adolfo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Investigaciones en Físico-química de Córdoba. Universidad Nacional de Córdoba. Facultad de Ciencias Químicas. Instituto de Investigaciones en Físico-química de Córdoba; ArgentinaFil: Rodriguez, Marcela Cecilia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Investigaciones en Físico-química de Córdoba. Universidad Nacional de Córdoba. Facultad de Ciencias Químicas. Instituto de Investigaciones en Físico-química de Córdoba; ArgentinaElsevier Science2012-09info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/26000Jalit, Yamile; Gutierrez, Fabiana Andrea; Dubacheva, Galina; Goyer, Cedric; Coche Guerente, Liliane; et al.; Characterization of a modified gold platform for the development of a label-free anti-thrombin aptasensor; Elsevier Science; Biosensors & Bioelectronics; 41; 9-2012; 424-4290956-5663CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.1016/j.bios.2012.08.061info:eu-repo/semantics/altIdentifier/url/http://www.sciencedirect.com/science/article/pii/S0956566312006021info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-nd/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T10:01:54Zoai:ri.conicet.gov.ar:11336/26000instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 10:01:55.235CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Characterization of a modified gold platform for the development of a label-free anti-thrombin aptasensor
title Characterization of a modified gold platform for the development of a label-free anti-thrombin aptasensor
spellingShingle Characterization of a modified gold platform for the development of a label-free anti-thrombin aptasensor
Jalit, Yamile
Thrombin Binding Aptamer
Aptasensor
Optical Aptasensor
Thrombin Biosensor
Protein Detection
Spr Sensing
title_short Characterization of a modified gold platform for the development of a label-free anti-thrombin aptasensor
title_full Characterization of a modified gold platform for the development of a label-free anti-thrombin aptasensor
title_fullStr Characterization of a modified gold platform for the development of a label-free anti-thrombin aptasensor
title_full_unstemmed Characterization of a modified gold platform for the development of a label-free anti-thrombin aptasensor
title_sort Characterization of a modified gold platform for the development of a label-free anti-thrombin aptasensor
dc.creator.none.fl_str_mv Jalit, Yamile
Gutierrez, Fabiana Andrea
Dubacheva, Galina
Goyer, Cedric
Coche Guerente, Liliane
Defrancq, Eric
Labbe, Pierre
Rivas, Gustavo Adolfo
Rodriguez, Marcela Cecilia
author Jalit, Yamile
author_facet Jalit, Yamile
Gutierrez, Fabiana Andrea
Dubacheva, Galina
Goyer, Cedric
Coche Guerente, Liliane
Defrancq, Eric
Labbe, Pierre
Rivas, Gustavo Adolfo
Rodriguez, Marcela Cecilia
author_role author
author2 Gutierrez, Fabiana Andrea
Dubacheva, Galina
Goyer, Cedric
Coche Guerente, Liliane
Defrancq, Eric
Labbe, Pierre
Rivas, Gustavo Adolfo
Rodriguez, Marcela Cecilia
author2_role author
author
author
author
author
author
author
author
dc.subject.none.fl_str_mv Thrombin Binding Aptamer
Aptasensor
Optical Aptasensor
Thrombin Biosensor
Protein Detection
Spr Sensing
topic Thrombin Binding Aptamer
Aptasensor
Optical Aptasensor
Thrombin Biosensor
Protein Detection
Spr Sensing
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.4
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv This work reports the characterization of a modified gold surface as a platform for the development of a label free aptasensor for thrombin detection. The biorecognition platform was obtained by the self-assembly of 4-mercaptobenzoic acid onto a gold surface, covalent attachment of streptavidin and further immobilization of the biotinylated anti-thrombin aptamer. The biosensing platform was characterized by cyclic voltammetry, electrochemical impedance spectroscopy, surface plasmon resonance (SPR) and quartz crystal microbalance with dissipation monitoring. The biorecognition event aptamer-thrombin was detected from changes in the SPR angle produced as a consequence of the molecular interaction between the aptasensor and the target protein. The biosensing platform demonstrated to be highly selective for human thrombin even in the presence of large excess of bovine thrombin, bovine serum albumin, cytochrome C, lysozyme and myoglobin. The relationship between the changes in the SPR angle and thrombin concentration was linear up to 0.19 μmol L−1 (R2=0.992) while the detection limit was of 12.0 nmol L−1 (240 fmol in the sample). This new sensing approach represents an interesting and promising alternative for the SPR-based quantification of thrombin.
Fil: Jalit, Yamile. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Investigaciones en Físico-química de Córdoba. Universidad Nacional de Córdoba. Facultad de Ciencias Químicas. Instituto de Investigaciones en Físico-química de Córdoba; Argentina
Fil: Gutierrez, Fabiana Andrea. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Investigaciones en Físico-química de Córdoba. Universidad Nacional de Córdoba. Facultad de Ciencias Químicas. Instituto de Investigaciones en Físico-química de Córdoba; Argentina
Fil: Dubacheva, Galina. Universite Joseph Fourier; Francia
Fil: Goyer, Cedric. Universite Joseph Fourier; Francia
Fil: Coche Guerente, Liliane. Universite Joseph Fourier; Francia
Fil: Defrancq, Eric. Universite Joseph Fourier; Francia
Fil: Labbe, Pierre. Universite Joseph Fourier; Francia
Fil: Rivas, Gustavo Adolfo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Investigaciones en Físico-química de Córdoba. Universidad Nacional de Córdoba. Facultad de Ciencias Químicas. Instituto de Investigaciones en Físico-química de Córdoba; Argentina
Fil: Rodriguez, Marcela Cecilia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Investigaciones en Físico-química de Córdoba. Universidad Nacional de Córdoba. Facultad de Ciencias Químicas. Instituto de Investigaciones en Físico-química de Córdoba; Argentina
description This work reports the characterization of a modified gold surface as a platform for the development of a label free aptasensor for thrombin detection. The biorecognition platform was obtained by the self-assembly of 4-mercaptobenzoic acid onto a gold surface, covalent attachment of streptavidin and further immobilization of the biotinylated anti-thrombin aptamer. The biosensing platform was characterized by cyclic voltammetry, electrochemical impedance spectroscopy, surface plasmon resonance (SPR) and quartz crystal microbalance with dissipation monitoring. The biorecognition event aptamer-thrombin was detected from changes in the SPR angle produced as a consequence of the molecular interaction between the aptasensor and the target protein. The biosensing platform demonstrated to be highly selective for human thrombin even in the presence of large excess of bovine thrombin, bovine serum albumin, cytochrome C, lysozyme and myoglobin. The relationship between the changes in the SPR angle and thrombin concentration was linear up to 0.19 μmol L−1 (R2=0.992) while the detection limit was of 12.0 nmol L−1 (240 fmol in the sample). This new sensing approach represents an interesting and promising alternative for the SPR-based quantification of thrombin.
publishDate 2012
dc.date.none.fl_str_mv 2012-09
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/26000
Jalit, Yamile; Gutierrez, Fabiana Andrea; Dubacheva, Galina; Goyer, Cedric; Coche Guerente, Liliane; et al.; Characterization of a modified gold platform for the development of a label-free anti-thrombin aptasensor; Elsevier Science; Biosensors & Bioelectronics; 41; 9-2012; 424-429
0956-5663
CONICET Digital
CONICET
url http://hdl.handle.net/11336/26000
identifier_str_mv Jalit, Yamile; Gutierrez, Fabiana Andrea; Dubacheva, Galina; Goyer, Cedric; Coche Guerente, Liliane; et al.; Characterization of a modified gold platform for the development of a label-free anti-thrombin aptasensor; Elsevier Science; Biosensors & Bioelectronics; 41; 9-2012; 424-429
0956-5663
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.1016/j.bios.2012.08.061
info:eu-repo/semantics/altIdentifier/url/http://www.sciencedirect.com/science/article/pii/S0956566312006021
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Elsevier Science
publisher.none.fl_str_mv Elsevier Science
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.070432

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