Hindered cytoplasmic diffusion of inositol trisphosphate restricts its cellular range of action

Autores
Dickinson, George D.; Ellefsen, Kyle L.; Ponce Dawson, Silvina Martha; Pearson, John E.; Parker, Ian
Año de publicación
2016
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
The range of action of intracellular messengers is determined by their rates of diffusion and degradation. Previous measurements in oocyte cytoplasmic extracts indicated that the Ca2+-liberating secondmessenger inositol trisphosphate (IP3) diffuses with a coefficient (∼280 mm2 s-1) similar to that in water, corresponding to a range of action of ∼25 mm. Consequently, IP3 is generally considered a "global" cellular messenger. We reexamined this issue by measuring local IP3-evoked Ca2+ puffs to monitor IP3 diffusing from spot photorelease in neuroblastoma cells. Fitting these data by numerical simulations yielded a diffusion coefficient (≤10 mm2 s-1) about 30-fold slower than that previously reported. We propose that diffusion of IP3 in mammalian cells is hindered by binding to immobile, functionally inactive receptors that were diluted in oocyte extracts. The predicted range of action of IP3 (<5 mm) is thus smaller than the size of typical mammalian cells, indicating that IP3 should better be considered as a local rather than a global cellular messenger. 2016
Fil: Dickinson, George D.. University of California at Irvine; Estados Unidos
Fil: Ellefsen, Kyle L.. University of California at Irvine; Estados Unidos
Fil: Ponce Dawson, Silvina Martha. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Física de Buenos Aires. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Instituto de Física de Buenos Aires; Argentina. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Física; Argentina
Fil: Pearson, John E.. Los Alamos National Laboratory; Estados Unidos
Fil: Parker, Ian. University of California at Irvine; Estados Unidos
Materia
Ip3
Calcium
Diffusion
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/49523
Acceder
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network_name_str CONICET Digital (CONICET)
spelling Hindered cytoplasmic diffusion of inositol trisphosphate restricts its cellular range of actionDickinson, George D.Ellefsen, Kyle L.Ponce Dawson, Silvina MarthaPearson, John E.Parker, IanIp3CalciumDiffusionhttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1The range of action of intracellular messengers is determined by their rates of diffusion and degradation. Previous measurements in oocyte cytoplasmic extracts indicated that the Ca2+-liberating secondmessenger inositol trisphosphate (IP3) diffuses with a coefficient (∼280 mm2 s-1) similar to that in water, corresponding to a range of action of ∼25 mm. Consequently, IP3 is generally considered a "global" cellular messenger. We reexamined this issue by measuring local IP3-evoked Ca2+ puffs to monitor IP3 diffusing from spot photorelease in neuroblastoma cells. Fitting these data by numerical simulations yielded a diffusion coefficient (≤10 mm2 s-1) about 30-fold slower than that previously reported. We propose that diffusion of IP3 in mammalian cells is hindered by binding to immobile, functionally inactive receptors that were diluted in oocyte extracts. The predicted range of action of IP3 (<5 mm) is thus smaller than the size of typical mammalian cells, indicating that IP3 should better be considered as a local rather than a global cellular messenger. 2016Fil: Dickinson, George D.. University of California at Irvine; Estados UnidosFil: Ellefsen, Kyle L.. University of California at Irvine; Estados UnidosFil: Ponce Dawson, Silvina Martha. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Física de Buenos Aires. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Instituto de Física de Buenos Aires; Argentina. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Física; ArgentinaFil: Pearson, John E.. Los Alamos National Laboratory; Estados UnidosFil: Parker, Ian. University of California at Irvine; Estados UnidosAmerican Association for the Advancement of Science2016-11info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/49523Dickinson, George D.; Ellefsen, Kyle L.; Ponce Dawson, Silvina Martha; Pearson, John E.; Parker, Ian; Hindered cytoplasmic diffusion of inositol trisphosphate restricts its cellular range of action; American Association for the Advancement of Science; Science Signaling; 9; 453; 11-2016; 10801-108101937-9145CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/http://stke.sciencemag.org/content/9/453/ra108info:eu-repo/semantics/altIdentifier/doi/10.1126/scisignal.aag1625info:eu-repo/semantics/altIdentifier/url/https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6876694/info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-10-22T11:29:43Zoai:ri.conicet.gov.ar:11336/49523instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-10-22 11:29:44.027CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Hindered cytoplasmic diffusion of inositol trisphosphate restricts its cellular range of action
title Hindered cytoplasmic diffusion of inositol trisphosphate restricts its cellular range of action
spellingShingle Hindered cytoplasmic diffusion of inositol trisphosphate restricts its cellular range of action
Dickinson, George D.
Ip3
Calcium
Diffusion
title_short Hindered cytoplasmic diffusion of inositol trisphosphate restricts its cellular range of action
title_full Hindered cytoplasmic diffusion of inositol trisphosphate restricts its cellular range of action
title_fullStr Hindered cytoplasmic diffusion of inositol trisphosphate restricts its cellular range of action
title_full_unstemmed Hindered cytoplasmic diffusion of inositol trisphosphate restricts its cellular range of action
title_sort Hindered cytoplasmic diffusion of inositol trisphosphate restricts its cellular range of action
dc.creator.none.fl_str_mv Dickinson, George D.
Ellefsen, Kyle L.
Ponce Dawson, Silvina Martha
Pearson, John E.
Parker, Ian
author Dickinson, George D.
author_facet Dickinson, George D.
Ellefsen, Kyle L.
Ponce Dawson, Silvina Martha
Pearson, John E.
Parker, Ian
author_role author
author2 Ellefsen, Kyle L.
Ponce Dawson, Silvina Martha
Pearson, John E.
Parker, Ian
author2_role author
author
author
author
dc.subject.none.fl_str_mv Ip3
Calcium
Diffusion
topic Ip3
Calcium
Diffusion
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv The range of action of intracellular messengers is determined by their rates of diffusion and degradation. Previous measurements in oocyte cytoplasmic extracts indicated that the Ca2+-liberating secondmessenger inositol trisphosphate (IP3) diffuses with a coefficient (∼280 mm2 s-1) similar to that in water, corresponding to a range of action of ∼25 mm. Consequently, IP3 is generally considered a "global" cellular messenger. We reexamined this issue by measuring local IP3-evoked Ca2+ puffs to monitor IP3 diffusing from spot photorelease in neuroblastoma cells. Fitting these data by numerical simulations yielded a diffusion coefficient (≤10 mm2 s-1) about 30-fold slower than that previously reported. We propose that diffusion of IP3 in mammalian cells is hindered by binding to immobile, functionally inactive receptors that were diluted in oocyte extracts. The predicted range of action of IP3 (<5 mm) is thus smaller than the size of typical mammalian cells, indicating that IP3 should better be considered as a local rather than a global cellular messenger. 2016
Fil: Dickinson, George D.. University of California at Irvine; Estados Unidos
Fil: Ellefsen, Kyle L.. University of California at Irvine; Estados Unidos
Fil: Ponce Dawson, Silvina Martha. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Física de Buenos Aires. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Instituto de Física de Buenos Aires; Argentina. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Física; Argentina
Fil: Pearson, John E.. Los Alamos National Laboratory; Estados Unidos
Fil: Parker, Ian. University of California at Irvine; Estados Unidos
description The range of action of intracellular messengers is determined by their rates of diffusion and degradation. Previous measurements in oocyte cytoplasmic extracts indicated that the Ca2+-liberating secondmessenger inositol trisphosphate (IP3) diffuses with a coefficient (∼280 mm2 s-1) similar to that in water, corresponding to a range of action of ∼25 mm. Consequently, IP3 is generally considered a "global" cellular messenger. We reexamined this issue by measuring local IP3-evoked Ca2+ puffs to monitor IP3 diffusing from spot photorelease in neuroblastoma cells. Fitting these data by numerical simulations yielded a diffusion coefficient (≤10 mm2 s-1) about 30-fold slower than that previously reported. We propose that diffusion of IP3 in mammalian cells is hindered by binding to immobile, functionally inactive receptors that were diluted in oocyte extracts. The predicted range of action of IP3 (<5 mm) is thus smaller than the size of typical mammalian cells, indicating that IP3 should better be considered as a local rather than a global cellular messenger. 2016
publishDate 2016
dc.date.none.fl_str_mv 2016-11
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/49523
Dickinson, George D.; Ellefsen, Kyle L.; Ponce Dawson, Silvina Martha; Pearson, John E.; Parker, Ian; Hindered cytoplasmic diffusion of inositol trisphosphate restricts its cellular range of action; American Association for the Advancement of Science; Science Signaling; 9; 453; 11-2016; 10801-10810
1937-9145
CONICET Digital
CONICET
url http://hdl.handle.net/11336/49523
identifier_str_mv Dickinson, George D.; Ellefsen, Kyle L.; Ponce Dawson, Silvina Martha; Pearson, John E.; Parker, Ian; Hindered cytoplasmic diffusion of inositol trisphosphate restricts its cellular range of action; American Association for the Advancement of Science; Science Signaling; 9; 453; 11-2016; 10801-10810
1937-9145
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/http://stke.sciencemag.org/content/9/453/ra108
info:eu-repo/semantics/altIdentifier/doi/10.1126/scisignal.aag1625
info:eu-repo/semantics/altIdentifier/url/https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6876694/
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv American Association for the Advancement of Science
publisher.none.fl_str_mv American Association for the Advancement of Science
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 12.982451

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