Thyroid hormones-membrane interaction: Reversible association of hormones with organized phospholipids with changes in fluidity and dipole potential

Autores
Isse, Blanca Alicia de Los Angeles G.; Yunes Quartino, Pablo Javier; Fidelio, Gerardo Daniel; Farias, Ricardo Norberto
Año de publicación
2013
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Differential scanning calorimetry (DSC), mixed monomolecular layers and fluorescence spectroscopy techniques were applied to investigate the effect of thyroid hormones (THs) on the biophysical properties of model membranes. We found that both 3,3′,5-triiodo-l-thyronine (T3) and 3,5,3′,5′-tetraiodo-l-thyronine (T4) induce a broadening of the calorimetric main phase transition profile and reduce the transition enthalpy in liquid-crystalline state of dipalmitoylphosphatylcholine (DPPC) multilamellar vesicles. Tm changes from 41 °C to 40 °C compared to pure DPPC. When the experiments were done by adding THs to preformed multilamellar vesicles a second broader component in the DSC scan also appears at 20 min of incubation and becomes gradually more prominent with time, indicating a progressive alteration of lipid phase induced by THs. Analysis of surface pressure-molecular area isotherms in mixed monolayers of THs with either DPPC or 1-palmitoyl-2-oleoylphosphatidylcholine (POPC) at air–water interface indicated a reduction in molecular area for THs/lipid mixtures at all surface pressures. A substantial decrease in surface potential in mixed lipid/THs monolayers at all surface pressures were observed for both phospholipids without affecting the mixed monolayer integrity. The data of mixed lipid/THs behavior support the establishment of lateral miscibility. Alterations of bidimensional liquid expanded → liquid condensed phase transition observed for DPPC/THs mixed monolayers are compatible with the changes observed in DSC. The transverse movement of THs and the decrease of dipole potential were also observed in single unilamellar vesicles by using appropriate fluorescent probes.
Fil: Isse, Blanca Alicia de Los Angeles G.. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Instituto Superior de Investigaciones Biológicas. Universidad Nacional de Tucumán. Instituto Superior de Investigaciones Biológicas; Argentina
Fil: Yunes Quartino, Pablo Javier. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Centro de Investigaciones en Química Biológica de Córdoba. Universidad Nacional de Córdoba. Facultad de Ciencias Químicas. Centro de Investigaciones en Química Biológica de Córdoba; Argentina
Fil: Fidelio, Gerardo Daniel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Centro de Investigaciones en Química Biológica de Córdoba. Universidad Nacional de Córdoba. Facultad de Ciencias Químicas. Centro de Investigaciones en Química Biológica de Córdoba; Argentina
Fil: Farias, Ricardo Norberto. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Instituto Superior de Investigaciones Biológicas. Universidad Nacional de Tucumán. Instituto Superior de Investigaciones Biológicas; Argentina
Materia
Thyroid Hormones
Phospholipids
Fluidity
Dipole Potential
Monomolecular Layers
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/26105
Acceder
id CONICETDig_594367dd6322feb506217081f9259dfa
oai_identifier_str oai:ri.conicet.gov.ar:11336/26105
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Thyroid hormones-membrane interaction: Reversible association of hormones with organized phospholipids with changes in fluidity and dipole potentialIsse, Blanca Alicia de Los Angeles G.Yunes Quartino, Pablo JavierFidelio, Gerardo DanielFarias, Ricardo NorbertoThyroid HormonesPhospholipidsFluidityDipole PotentialMonomolecular Layershttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1Differential scanning calorimetry (DSC), mixed monomolecular layers and fluorescence spectroscopy techniques were applied to investigate the effect of thyroid hormones (THs) on the biophysical properties of model membranes. We found that both 3,3′,5-triiodo-l-thyronine (T3) and 3,5,3′,5′-tetraiodo-l-thyronine (T4) induce a broadening of the calorimetric main phase transition profile and reduce the transition enthalpy in liquid-crystalline state of dipalmitoylphosphatylcholine (DPPC) multilamellar vesicles. Tm changes from 41 °C to 40 °C compared to pure DPPC. When the experiments were done by adding THs to preformed multilamellar vesicles a second broader component in the DSC scan also appears at 20 min of incubation and becomes gradually more prominent with time, indicating a progressive alteration of lipid phase induced by THs. Analysis of surface pressure-molecular area isotherms in mixed monolayers of THs with either DPPC or 1-palmitoyl-2-oleoylphosphatidylcholine (POPC) at air–water interface indicated a reduction in molecular area for THs/lipid mixtures at all surface pressures. A substantial decrease in surface potential in mixed lipid/THs monolayers at all surface pressures were observed for both phospholipids without affecting the mixed monolayer integrity. The data of mixed lipid/THs behavior support the establishment of lateral miscibility. Alterations of bidimensional liquid expanded → liquid condensed phase transition observed for DPPC/THs mixed monolayers are compatible with the changes observed in DSC. The transverse movement of THs and the decrease of dipole potential were also observed in single unilamellar vesicles by using appropriate fluorescent probes.Fil: Isse, Blanca Alicia de Los Angeles G.. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Instituto Superior de Investigaciones Biológicas. Universidad Nacional de Tucumán. Instituto Superior de Investigaciones Biológicas; ArgentinaFil: Yunes Quartino, Pablo Javier. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Centro de Investigaciones en Química Biológica de Córdoba. Universidad Nacional de Córdoba. Facultad de Ciencias Químicas. Centro de Investigaciones en Química Biológica de Córdoba; ArgentinaFil: Fidelio, Gerardo Daniel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Centro de Investigaciones en Química Biológica de Córdoba. Universidad Nacional de Córdoba. Facultad de Ciencias Químicas. Centro de Investigaciones en Química Biológica de Córdoba; ArgentinaFil: Farias, Ricardo Norberto. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Instituto Superior de Investigaciones Biológicas. Universidad Nacional de Tucumán. Instituto Superior de Investigaciones Biológicas; ArgentinaElsevier Ireland2013-09info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/26105Isse, Blanca Alicia de Los Angeles G.; Yunes Quartino, Pablo Javier; Fidelio, Gerardo Daniel; Farias, Ricardo Norberto; Thyroid hormones-membrane interaction: Reversible association of hormones with organized phospholipids with changes in fluidity and dipole potential; Elsevier Ireland; Chemistry And Physics Of Lipids; 175-176; 9-2013; 131-1370009-3084CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.1016/j.chemphyslip.2013.08.007info:eu-repo/semantics/altIdentifier/url/http://www.sciencedirect.com/science/article/pii/S0009308413001205info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-nd/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-10-15T14:58:54Zoai:ri.conicet.gov.ar:11336/26105instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-10-15 14:58:55.027CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Thyroid hormones-membrane interaction: Reversible association of hormones with organized phospholipids with changes in fluidity and dipole potential
title Thyroid hormones-membrane interaction: Reversible association of hormones with organized phospholipids with changes in fluidity and dipole potential
spellingShingle Thyroid hormones-membrane interaction: Reversible association of hormones with organized phospholipids with changes in fluidity and dipole potential
Isse, Blanca Alicia de Los Angeles G.
Thyroid Hormones
Phospholipids
Fluidity
Dipole Potential
Monomolecular Layers
title_short Thyroid hormones-membrane interaction: Reversible association of hormones with organized phospholipids with changes in fluidity and dipole potential
title_full Thyroid hormones-membrane interaction: Reversible association of hormones with organized phospholipids with changes in fluidity and dipole potential
title_fullStr Thyroid hormones-membrane interaction: Reversible association of hormones with organized phospholipids with changes in fluidity and dipole potential
title_full_unstemmed Thyroid hormones-membrane interaction: Reversible association of hormones with organized phospholipids with changes in fluidity and dipole potential
title_sort Thyroid hormones-membrane interaction: Reversible association of hormones with organized phospholipids with changes in fluidity and dipole potential
dc.creator.none.fl_str_mv Isse, Blanca Alicia de Los Angeles G.
Yunes Quartino, Pablo Javier
Fidelio, Gerardo Daniel
Farias, Ricardo Norberto
author Isse, Blanca Alicia de Los Angeles G.
author_facet Isse, Blanca Alicia de Los Angeles G.
Yunes Quartino, Pablo Javier
Fidelio, Gerardo Daniel
Farias, Ricardo Norberto
author_role author
author2 Yunes Quartino, Pablo Javier
Fidelio, Gerardo Daniel
Farias, Ricardo Norberto
author2_role author
author
author
dc.subject.none.fl_str_mv Thyroid Hormones
Phospholipids
Fluidity
Dipole Potential
Monomolecular Layers
topic Thyroid Hormones
Phospholipids
Fluidity
Dipole Potential
Monomolecular Layers
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv Differential scanning calorimetry (DSC), mixed monomolecular layers and fluorescence spectroscopy techniques were applied to investigate the effect of thyroid hormones (THs) on the biophysical properties of model membranes. We found that both 3,3′,5-triiodo-l-thyronine (T3) and 3,5,3′,5′-tetraiodo-l-thyronine (T4) induce a broadening of the calorimetric main phase transition profile and reduce the transition enthalpy in liquid-crystalline state of dipalmitoylphosphatylcholine (DPPC) multilamellar vesicles. Tm changes from 41 °C to 40 °C compared to pure DPPC. When the experiments were done by adding THs to preformed multilamellar vesicles a second broader component in the DSC scan also appears at 20 min of incubation and becomes gradually more prominent with time, indicating a progressive alteration of lipid phase induced by THs. Analysis of surface pressure-molecular area isotherms in mixed monolayers of THs with either DPPC or 1-palmitoyl-2-oleoylphosphatidylcholine (POPC) at air–water interface indicated a reduction in molecular area for THs/lipid mixtures at all surface pressures. A substantial decrease in surface potential in mixed lipid/THs monolayers at all surface pressures were observed for both phospholipids without affecting the mixed monolayer integrity. The data of mixed lipid/THs behavior support the establishment of lateral miscibility. Alterations of bidimensional liquid expanded → liquid condensed phase transition observed for DPPC/THs mixed monolayers are compatible with the changes observed in DSC. The transverse movement of THs and the decrease of dipole potential were also observed in single unilamellar vesicles by using appropriate fluorescent probes.
Fil: Isse, Blanca Alicia de Los Angeles G.. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Instituto Superior de Investigaciones Biológicas. Universidad Nacional de Tucumán. Instituto Superior de Investigaciones Biológicas; Argentina
Fil: Yunes Quartino, Pablo Javier. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Centro de Investigaciones en Química Biológica de Córdoba. Universidad Nacional de Córdoba. Facultad de Ciencias Químicas. Centro de Investigaciones en Química Biológica de Córdoba; Argentina
Fil: Fidelio, Gerardo Daniel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Centro de Investigaciones en Química Biológica de Córdoba. Universidad Nacional de Córdoba. Facultad de Ciencias Químicas. Centro de Investigaciones en Química Biológica de Córdoba; Argentina
Fil: Farias, Ricardo Norberto. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Instituto Superior de Investigaciones Biológicas. Universidad Nacional de Tucumán. Instituto Superior de Investigaciones Biológicas; Argentina
description Differential scanning calorimetry (DSC), mixed monomolecular layers and fluorescence spectroscopy techniques were applied to investigate the effect of thyroid hormones (THs) on the biophysical properties of model membranes. We found that both 3,3′,5-triiodo-l-thyronine (T3) and 3,5,3′,5′-tetraiodo-l-thyronine (T4) induce a broadening of the calorimetric main phase transition profile and reduce the transition enthalpy in liquid-crystalline state of dipalmitoylphosphatylcholine (DPPC) multilamellar vesicles. Tm changes from 41 °C to 40 °C compared to pure DPPC. When the experiments were done by adding THs to preformed multilamellar vesicles a second broader component in the DSC scan also appears at 20 min of incubation and becomes gradually more prominent with time, indicating a progressive alteration of lipid phase induced by THs. Analysis of surface pressure-molecular area isotherms in mixed monolayers of THs with either DPPC or 1-palmitoyl-2-oleoylphosphatidylcholine (POPC) at air–water interface indicated a reduction in molecular area for THs/lipid mixtures at all surface pressures. A substantial decrease in surface potential in mixed lipid/THs monolayers at all surface pressures were observed for both phospholipids without affecting the mixed monolayer integrity. The data of mixed lipid/THs behavior support the establishment of lateral miscibility. Alterations of bidimensional liquid expanded → liquid condensed phase transition observed for DPPC/THs mixed monolayers are compatible with the changes observed in DSC. The transverse movement of THs and the decrease of dipole potential were also observed in single unilamellar vesicles by using appropriate fluorescent probes.
publishDate 2013
dc.date.none.fl_str_mv 2013-09
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/26105
Isse, Blanca Alicia de Los Angeles G.; Yunes Quartino, Pablo Javier; Fidelio, Gerardo Daniel; Farias, Ricardo Norberto; Thyroid hormones-membrane interaction: Reversible association of hormones with organized phospholipids with changes in fluidity and dipole potential; Elsevier Ireland; Chemistry And Physics Of Lipids; 175-176; 9-2013; 131-137
0009-3084
CONICET Digital
CONICET
url http://hdl.handle.net/11336/26105
identifier_str_mv Isse, Blanca Alicia de Los Angeles G.; Yunes Quartino, Pablo Javier; Fidelio, Gerardo Daniel; Farias, Ricardo Norberto; Thyroid hormones-membrane interaction: Reversible association of hormones with organized phospholipids with changes in fluidity and dipole potential; Elsevier Ireland; Chemistry And Physics Of Lipids; 175-176; 9-2013; 131-137
0009-3084
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.1016/j.chemphyslip.2013.08.007
info:eu-repo/semantics/altIdentifier/url/http://www.sciencedirect.com/science/article/pii/S0009308413001205
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Elsevier Ireland
publisher.none.fl_str_mv Elsevier Ireland
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.22299

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