Determination of proviral load in bovine leukemia virus–infected cattle with and without lymphocytosis

Autores
Juliarena, Marcela Alicia; Gutiérrez, Silvina Elena; Ceriani, Maria Carolina
Año de publicación
2007
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Objective: To determine proviral load in bovine leukemia virus (BLV)–infected cattle with and without persistent lymphocytosis to assess the potential of transmitting the virus. Animals: Cattle in 6 dairy herds. Procedures: Blood samples from infected cows were evaluated 3 times at 6-month intervals for determination of proviral load via PCR assay, serologic results via ELISA, and hematologic status via differential cell counts. Results: Infected cattle were classified into lymphocytotic and nonlymphocytotic groups. Lymphocytotic cattle consistently had > 100,000 copies of integrated provirus/µg of DNA (ie, high proviral load) in peripheral blood leukocytes. Titers of antibodies against BLVgp51 and BLVp24 indicated a strong immune response. Nonlymphocytotic cattle comprised 2 subgroups: a group with high proviral load and strong immune response, and a group with a weaker immune response, mostly against BLVp24, and a proviral load of < 100 copies/µg of DNA (ie, low proviral load). Conclusions and Clinical Relevance: Results emphasized the importance of characterizing nonlymphocytotic BLV-infected cattle during eradication programs. The risk of transmitting BLV infection from nonlymphocytotic cattle may differ depending on the proviral load. Nonlymphocytotic cattle with high proviral load could be efficient transmitters (as efficient as lymphocytotic cattle), whereas nonlymphocytotic cattle with low proviral load could be inefficient transmitters under standard husbandry conditions. Because most cattle with low proviral load do not develop anti-BLVp24 antibodies, it appears that lack of an anti- BLVp24 antibody response may be a good marker of this condition.
Fil: Juliarena, Marcela Alicia. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional del Centro de la Provincia de Buenos Aires. Facultad de Ciencias Veterinarias. Departamento de Sanidad Animal y Medicina Preventiva. Laboratorio de Virologia; Argentina
Fil: Gutiérrez, Silvina Elena. Universidad Nacional del Centro de la Provincia de Buenos Aires. Facultad de Ciencias Veterinarias. Departamento de Sanidad Animal y Medicina Preventiva. Laboratorio de Virologia; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Ceriani, Maria Carolina. Universidad Nacional del Centro de la Provincia de Buenos Aires. Facultad de Ciencias Veterinarias. Departamento de Sanidad Animal y Medicina Preventiva. Laboratorio de Virologia; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Materia
BOVINE LEUKEMIA VIRUS
PROVIRAL LOAD
ANTIBODIES
PERSISTENT LYMPHOCYTOSIS
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/103808
Acceder
id CONICETDig_593a211063f05a7340f5a26b2946b68a
oai_identifier_str oai:ri.conicet.gov.ar:11336/103808
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Determination of proviral load in bovine leukemia virus–infected cattle with and without lymphocytosisJuliarena, Marcela AliciaGutiérrez, Silvina ElenaCeriani, Maria CarolinaBOVINE LEUKEMIA VIRUSPROVIRAL LOADANTIBODIESPERSISTENT LYMPHOCYTOSIShttps://purl.org/becyt/ford/4.3https://purl.org/becyt/ford/4Objective: To determine proviral load in bovine leukemia virus (BLV)–infected cattle with and without persistent lymphocytosis to assess the potential of transmitting the virus. Animals: Cattle in 6 dairy herds. Procedures: Blood samples from infected cows were evaluated 3 times at 6-month intervals for determination of proviral load via PCR assay, serologic results via ELISA, and hematologic status via differential cell counts. Results: Infected cattle were classified into lymphocytotic and nonlymphocytotic groups. Lymphocytotic cattle consistently had > 100,000 copies of integrated provirus/µg of DNA (ie, high proviral load) in peripheral blood leukocytes. Titers of antibodies against BLVgp51 and BLVp24 indicated a strong immune response. Nonlymphocytotic cattle comprised 2 subgroups: a group with high proviral load and strong immune response, and a group with a weaker immune response, mostly against BLVp24, and a proviral load of < 100 copies/µg of DNA (ie, low proviral load). Conclusions and Clinical Relevance: Results emphasized the importance of characterizing nonlymphocytotic BLV-infected cattle during eradication programs. The risk of transmitting BLV infection from nonlymphocytotic cattle may differ depending on the proviral load. Nonlymphocytotic cattle with high proviral load could be efficient transmitters (as efficient as lymphocytotic cattle), whereas nonlymphocytotic cattle with low proviral load could be inefficient transmitters under standard husbandry conditions. Because most cattle with low proviral load do not develop anti-BLVp24 antibodies, it appears that lack of an anti- BLVp24 antibody response may be a good marker of this condition.Fil: Juliarena, Marcela Alicia. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional del Centro de la Provincia de Buenos Aires. Facultad de Ciencias Veterinarias. Departamento de Sanidad Animal y Medicina Preventiva. Laboratorio de Virologia; ArgentinaFil: Gutiérrez, Silvina Elena. Universidad Nacional del Centro de la Provincia de Buenos Aires. Facultad de Ciencias Veterinarias. Departamento de Sanidad Animal y Medicina Preventiva. Laboratorio de Virologia; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Ceriani, Maria Carolina. Universidad Nacional del Centro de la Provincia de Buenos Aires. Facultad de Ciencias Veterinarias. Departamento de Sanidad Animal y Medicina Preventiva. Laboratorio de Virologia; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaAmerican Veterinary Medical Association2007-11info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/103808Juliarena, Marcela Alicia; Gutiérrez, Silvina Elena; Ceriani, Maria Carolina; Determination of proviral load in bovine leukemia virus–infected cattle with and without lymphocytosis; American Veterinary Medical Association; American Journal of Veterinary Research; 68; 11; 11-2007; 1220-12250002-96451943-5681CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.2460/ajvr.68.11.1220info:eu-repo/semantics/altIdentifier/url/https://avmajournals.avma.org/doi/abs/10.2460/ajvr.68.11.1220info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-03T09:54:42Zoai:ri.conicet.gov.ar:11336/103808instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-03 09:54:43.238CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Determination of proviral load in bovine leukemia virus–infected cattle with and without lymphocytosis
title Determination of proviral load in bovine leukemia virus–infected cattle with and without lymphocytosis
spellingShingle Determination of proviral load in bovine leukemia virus–infected cattle with and without lymphocytosis
Juliarena, Marcela Alicia
BOVINE LEUKEMIA VIRUS
PROVIRAL LOAD
ANTIBODIES
PERSISTENT LYMPHOCYTOSIS
title_short Determination of proviral load in bovine leukemia virus–infected cattle with and without lymphocytosis
title_full Determination of proviral load in bovine leukemia virus–infected cattle with and without lymphocytosis
title_fullStr Determination of proviral load in bovine leukemia virus–infected cattle with and without lymphocytosis
title_full_unstemmed Determination of proviral load in bovine leukemia virus–infected cattle with and without lymphocytosis
title_sort Determination of proviral load in bovine leukemia virus–infected cattle with and without lymphocytosis
dc.creator.none.fl_str_mv Juliarena, Marcela Alicia
Gutiérrez, Silvina Elena
Ceriani, Maria Carolina
author Juliarena, Marcela Alicia
author_facet Juliarena, Marcela Alicia
Gutiérrez, Silvina Elena
Ceriani, Maria Carolina
author_role author
author2 Gutiérrez, Silvina Elena
Ceriani, Maria Carolina
author2_role author
author
dc.subject.none.fl_str_mv BOVINE LEUKEMIA VIRUS
PROVIRAL LOAD
ANTIBODIES
PERSISTENT LYMPHOCYTOSIS
topic BOVINE LEUKEMIA VIRUS
PROVIRAL LOAD
ANTIBODIES
PERSISTENT LYMPHOCYTOSIS
purl_subject.fl_str_mv https://purl.org/becyt/ford/4.3
https://purl.org/becyt/ford/4
dc.description.none.fl_txt_mv Objective: To determine proviral load in bovine leukemia virus (BLV)–infected cattle with and without persistent lymphocytosis to assess the potential of transmitting the virus. Animals: Cattle in 6 dairy herds. Procedures: Blood samples from infected cows were evaluated 3 times at 6-month intervals for determination of proviral load via PCR assay, serologic results via ELISA, and hematologic status via differential cell counts. Results: Infected cattle were classified into lymphocytotic and nonlymphocytotic groups. Lymphocytotic cattle consistently had > 100,000 copies of integrated provirus/µg of DNA (ie, high proviral load) in peripheral blood leukocytes. Titers of antibodies against BLVgp51 and BLVp24 indicated a strong immune response. Nonlymphocytotic cattle comprised 2 subgroups: a group with high proviral load and strong immune response, and a group with a weaker immune response, mostly against BLVp24, and a proviral load of < 100 copies/µg of DNA (ie, low proviral load). Conclusions and Clinical Relevance: Results emphasized the importance of characterizing nonlymphocytotic BLV-infected cattle during eradication programs. The risk of transmitting BLV infection from nonlymphocytotic cattle may differ depending on the proviral load. Nonlymphocytotic cattle with high proviral load could be efficient transmitters (as efficient as lymphocytotic cattle), whereas nonlymphocytotic cattle with low proviral load could be inefficient transmitters under standard husbandry conditions. Because most cattle with low proviral load do not develop anti-BLVp24 antibodies, it appears that lack of an anti- BLVp24 antibody response may be a good marker of this condition.
Fil: Juliarena, Marcela Alicia. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional del Centro de la Provincia de Buenos Aires. Facultad de Ciencias Veterinarias. Departamento de Sanidad Animal y Medicina Preventiva. Laboratorio de Virologia; Argentina
Fil: Gutiérrez, Silvina Elena. Universidad Nacional del Centro de la Provincia de Buenos Aires. Facultad de Ciencias Veterinarias. Departamento de Sanidad Animal y Medicina Preventiva. Laboratorio de Virologia; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Ceriani, Maria Carolina. Universidad Nacional del Centro de la Provincia de Buenos Aires. Facultad de Ciencias Veterinarias. Departamento de Sanidad Animal y Medicina Preventiva. Laboratorio de Virologia; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
description Objective: To determine proviral load in bovine leukemia virus (BLV)–infected cattle with and without persistent lymphocytosis to assess the potential of transmitting the virus. Animals: Cattle in 6 dairy herds. Procedures: Blood samples from infected cows were evaluated 3 times at 6-month intervals for determination of proviral load via PCR assay, serologic results via ELISA, and hematologic status via differential cell counts. Results: Infected cattle were classified into lymphocytotic and nonlymphocytotic groups. Lymphocytotic cattle consistently had > 100,000 copies of integrated provirus/µg of DNA (ie, high proviral load) in peripheral blood leukocytes. Titers of antibodies against BLVgp51 and BLVp24 indicated a strong immune response. Nonlymphocytotic cattle comprised 2 subgroups: a group with high proviral load and strong immune response, and a group with a weaker immune response, mostly against BLVp24, and a proviral load of < 100 copies/µg of DNA (ie, low proviral load). Conclusions and Clinical Relevance: Results emphasized the importance of characterizing nonlymphocytotic BLV-infected cattle during eradication programs. The risk of transmitting BLV infection from nonlymphocytotic cattle may differ depending on the proviral load. Nonlymphocytotic cattle with high proviral load could be efficient transmitters (as efficient as lymphocytotic cattle), whereas nonlymphocytotic cattle with low proviral load could be inefficient transmitters under standard husbandry conditions. Because most cattle with low proviral load do not develop anti-BLVp24 antibodies, it appears that lack of an anti- BLVp24 antibody response may be a good marker of this condition.
publishDate 2007
dc.date.none.fl_str_mv 2007-11
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/103808
Juliarena, Marcela Alicia; Gutiérrez, Silvina Elena; Ceriani, Maria Carolina; Determination of proviral load in bovine leukemia virus–infected cattle with and without lymphocytosis; American Veterinary Medical Association; American Journal of Veterinary Research; 68; 11; 11-2007; 1220-1225
0002-9645
1943-5681
CONICET Digital
CONICET
url http://hdl.handle.net/11336/103808
identifier_str_mv Juliarena, Marcela Alicia; Gutiérrez, Silvina Elena; Ceriani, Maria Carolina; Determination of proviral load in bovine leukemia virus–infected cattle with and without lymphocytosis; American Veterinary Medical Association; American Journal of Veterinary Research; 68; 11; 11-2007; 1220-1225
0002-9645
1943-5681
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.2460/ajvr.68.11.1220
info:eu-repo/semantics/altIdentifier/url/https://avmajournals.avma.org/doi/abs/10.2460/ajvr.68.11.1220
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv American Veterinary Medical Association
publisher.none.fl_str_mv American Veterinary Medical Association
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.13397

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