Transcriptomic analysis in blv-experimentally infected cattle leading to high or low proviral load

Autores
Juliarena, Marcela Alicia; Forletti, Agustina; Lützelschwab, Claudia María; Gutiérrez, Silvina Elena
Año de publicación
2018
Idioma
inglés
Tipo de recurso
documento de conferencia
Estado
versión publicada
Descripción
Bovine Leukemia Virus (BLV) is a highly prevalent pathogen causing a fatal lymphoproliferative disease in the bovine species. Afterexperimental infection, proviral load peaks at 30 days post infection (dpi), then, cattle progress to two different phenotypes: one is characterizedby high proviral load (HPL) in peripheral blood, while the other is identified by low proviral load (LPL) in peripheral blood. In LPL cattle asharp decrease in proviral load is evidenced at 38 dpi. We studied the transcriptome in peripheral blood cells from 10 cattle (5 of each phenotype)infected with BLV at 38 dpi, to identify the host genes differentially expressed in those animals that progress to LPL, recognized as BLVresistant.RNA seq experiments showed 499 genes differentially expressed (p<0.05) between both phenotypes: 281 upregulated and 218downregulated in LPL compared to HPL cattle. Gene ontology analysis revealed that genes related to inflammatory response, humoral immuneresponse and leukocyte migration were up regulated in cattle progressing to LPL compared to HPL (p< 0.05), while the regulation of homeostaticprocess, antigen receptor signaling pathway and phospholipid transport were downregulated in LPL compared to HPL cattle (p< 0.05). The hugedifference in transcript expression found at 38 dpi, suggests that mechanisms used to control the proviral load are turned on early after theinfection, although the phenotype of LPL or HPL is established only after 90 dpi.
Fil: Juliarena, Marcela Alicia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tandil. Centro de Investigación Veterinaria de Tandil. Universidad Nacional del Centro de la Provincia de Buenos Aires. Centro de Investigación Veterinaria de Tandil. Provincia de Buenos Aires. Gobernación. Comision de Investigaciones Científicas. Centro de Investigación Veterinaria de Tandil; Argentina
Fil: Forletti, Agustina. Laboratorio Biologico de Tandil Srl.; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tandil. Centro de Investigación Veterinaria de Tandil. Universidad Nacional del Centro de la Provincia de Buenos Aires. Centro de Investigación Veterinaria de Tandil. Provincia de Buenos Aires. Gobernación. Comision de Investigaciones Científicas. Centro de Investigación Veterinaria de Tandil; Argentina
Fil: Lützelschwab, Claudia María. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tandil. Centro de Investigación Veterinaria de Tandil. Universidad Nacional del Centro de la Provincia de Buenos Aires. Centro de Investigación Veterinaria de Tandil. Provincia de Buenos Aires. Gobernación. Comision de Investigaciones Científicas. Centro de Investigación Veterinaria de Tandil; Argentina
Fil: Gutiérrez, Silvina Elena. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tandil. Centro de Investigación Veterinaria de Tandil. Universidad Nacional del Centro de la Provincia de Buenos Aires. Centro de Investigación Veterinaria de Tandil. Provincia de Buenos Aires. Gobernación. Comision de Investigaciones Científicas. Centro de Investigación Veterinaria de Tandil; Argentina
54th Annual Meeting Argentine Society for Biochemistry and Molecular Biology
Paraná
Argentina
Sociedad Argentina de Investigación en Bioquímica y Biología Molecular
Materia
BOVINE LEUKEMIA VIRUS
PROVIRAL LOAD
RESISTANCE
RNA SEQ
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/233128
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/233128
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Transcriptomic analysis in blv-experimentally infected cattle leading to high or low proviral loadJuliarena, Marcela AliciaForletti, AgustinaLützelschwab, Claudia MaríaGutiérrez, Silvina ElenaBOVINE LEUKEMIA VIRUSPROVIRAL LOADRESISTANCERNA SEQhttps://purl.org/becyt/ford/4.3https://purl.org/becyt/ford/4Bovine Leukemia Virus (BLV) is a highly prevalent pathogen causing a fatal lymphoproliferative disease in the bovine species. Afterexperimental infection, proviral load peaks at 30 days post infection (dpi), then, cattle progress to two different phenotypes: one is characterizedby high proviral load (HPL) in peripheral blood, while the other is identified by low proviral load (LPL) in peripheral blood. In LPL cattle asharp decrease in proviral load is evidenced at 38 dpi. We studied the transcriptome in peripheral blood cells from 10 cattle (5 of each phenotype)infected with BLV at 38 dpi, to identify the host genes differentially expressed in those animals that progress to LPL, recognized as BLVresistant.RNA seq experiments showed 499 genes differentially expressed (p<0.05) between both phenotypes: 281 upregulated and 218downregulated in LPL compared to HPL cattle. Gene ontology analysis revealed that genes related to inflammatory response, humoral immuneresponse and leukocyte migration were up regulated in cattle progressing to LPL compared to HPL (p< 0.05), while the regulation of homeostaticprocess, antigen receptor signaling pathway and phospholipid transport were downregulated in LPL compared to HPL cattle (p< 0.05). The hugedifference in transcript expression found at 38 dpi, suggests that mechanisms used to control the proviral load are turned on early after theinfection, although the phenotype of LPL or HPL is established only after 90 dpi.Fil: Juliarena, Marcela Alicia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tandil. Centro de Investigación Veterinaria de Tandil. Universidad Nacional del Centro de la Provincia de Buenos Aires. Centro de Investigación Veterinaria de Tandil. Provincia de Buenos Aires. Gobernación. Comision de Investigaciones Científicas. Centro de Investigación Veterinaria de Tandil; ArgentinaFil: Forletti, Agustina. Laboratorio Biologico de Tandil Srl.; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tandil. Centro de Investigación Veterinaria de Tandil. Universidad Nacional del Centro de la Provincia de Buenos Aires. Centro de Investigación Veterinaria de Tandil. Provincia de Buenos Aires. Gobernación. Comision de Investigaciones Científicas. Centro de Investigación Veterinaria de Tandil; ArgentinaFil: Lützelschwab, Claudia María. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tandil. Centro de Investigación Veterinaria de Tandil. Universidad Nacional del Centro de la Provincia de Buenos Aires. Centro de Investigación Veterinaria de Tandil. Provincia de Buenos Aires. Gobernación. Comision de Investigaciones Científicas. Centro de Investigación Veterinaria de Tandil; ArgentinaFil: Gutiérrez, Silvina Elena. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tandil. Centro de Investigación Veterinaria de Tandil. Universidad Nacional del Centro de la Provincia de Buenos Aires. Centro de Investigación Veterinaria de Tandil. Provincia de Buenos Aires. Gobernación. Comision de Investigaciones Científicas. Centro de Investigación Veterinaria de Tandil; Argentina54th Annual Meeting Argentine Society for Biochemistry and Molecular BiologyParanáArgentinaSociedad Argentina de Investigación en Bioquímica y Biología MolecularTech Science Press2018info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/conferenceObjectReuniónJournalhttp://purl.org/coar/resource_type/c_5794info:ar-repo/semantics/documentoDeConferenciaapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/233128Transcriptomic analysis in blv-experimentally infected cattle leading to high or low proviral load; 54th Annual Meeting Argentine Society for Biochemistry and Molecular Biology; Paraná; Argentina; 2018; 1-51667-5746CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/http://www.saib.org.ar/sites/default/files/BIOCELL-SAIB-2018.pdfInternacionalinfo:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-03T09:51:06Zoai:ri.conicet.gov.ar:11336/233128instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-03 09:51:07.116CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Transcriptomic analysis in blv-experimentally infected cattle leading to high or low proviral load
title Transcriptomic analysis in blv-experimentally infected cattle leading to high or low proviral load
spellingShingle Transcriptomic analysis in blv-experimentally infected cattle leading to high or low proviral load
Juliarena, Marcela Alicia
BOVINE LEUKEMIA VIRUS
PROVIRAL LOAD
RESISTANCE
RNA SEQ
title_short Transcriptomic analysis in blv-experimentally infected cattle leading to high or low proviral load
title_full Transcriptomic analysis in blv-experimentally infected cattle leading to high or low proviral load
title_fullStr Transcriptomic analysis in blv-experimentally infected cattle leading to high or low proviral load
title_full_unstemmed Transcriptomic analysis in blv-experimentally infected cattle leading to high or low proviral load
title_sort Transcriptomic analysis in blv-experimentally infected cattle leading to high or low proviral load
dc.creator.none.fl_str_mv Juliarena, Marcela Alicia
Forletti, Agustina
Lützelschwab, Claudia María
Gutiérrez, Silvina Elena
author Juliarena, Marcela Alicia
author_facet Juliarena, Marcela Alicia
Forletti, Agustina
Lützelschwab, Claudia María
Gutiérrez, Silvina Elena
author_role author
author2 Forletti, Agustina
Lützelschwab, Claudia María
Gutiérrez, Silvina Elena
author2_role author
author
author
dc.subject.none.fl_str_mv BOVINE LEUKEMIA VIRUS
PROVIRAL LOAD
RESISTANCE
RNA SEQ
topic BOVINE LEUKEMIA VIRUS
PROVIRAL LOAD
RESISTANCE
RNA SEQ
purl_subject.fl_str_mv https://purl.org/becyt/ford/4.3
https://purl.org/becyt/ford/4
dc.description.none.fl_txt_mv Bovine Leukemia Virus (BLV) is a highly prevalent pathogen causing a fatal lymphoproliferative disease in the bovine species. Afterexperimental infection, proviral load peaks at 30 days post infection (dpi), then, cattle progress to two different phenotypes: one is characterizedby high proviral load (HPL) in peripheral blood, while the other is identified by low proviral load (LPL) in peripheral blood. In LPL cattle asharp decrease in proviral load is evidenced at 38 dpi. We studied the transcriptome in peripheral blood cells from 10 cattle (5 of each phenotype)infected with BLV at 38 dpi, to identify the host genes differentially expressed in those animals that progress to LPL, recognized as BLVresistant.RNA seq experiments showed 499 genes differentially expressed (p<0.05) between both phenotypes: 281 upregulated and 218downregulated in LPL compared to HPL cattle. Gene ontology analysis revealed that genes related to inflammatory response, humoral immuneresponse and leukocyte migration were up regulated in cattle progressing to LPL compared to HPL (p< 0.05), while the regulation of homeostaticprocess, antigen receptor signaling pathway and phospholipid transport were downregulated in LPL compared to HPL cattle (p< 0.05). The hugedifference in transcript expression found at 38 dpi, suggests that mechanisms used to control the proviral load are turned on early after theinfection, although the phenotype of LPL or HPL is established only after 90 dpi.
Fil: Juliarena, Marcela Alicia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tandil. Centro de Investigación Veterinaria de Tandil. Universidad Nacional del Centro de la Provincia de Buenos Aires. Centro de Investigación Veterinaria de Tandil. Provincia de Buenos Aires. Gobernación. Comision de Investigaciones Científicas. Centro de Investigación Veterinaria de Tandil; Argentina
Fil: Forletti, Agustina. Laboratorio Biologico de Tandil Srl.; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tandil. Centro de Investigación Veterinaria de Tandil. Universidad Nacional del Centro de la Provincia de Buenos Aires. Centro de Investigación Veterinaria de Tandil. Provincia de Buenos Aires. Gobernación. Comision de Investigaciones Científicas. Centro de Investigación Veterinaria de Tandil; Argentina
Fil: Lützelschwab, Claudia María. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tandil. Centro de Investigación Veterinaria de Tandil. Universidad Nacional del Centro de la Provincia de Buenos Aires. Centro de Investigación Veterinaria de Tandil. Provincia de Buenos Aires. Gobernación. Comision de Investigaciones Científicas. Centro de Investigación Veterinaria de Tandil; Argentina
Fil: Gutiérrez, Silvina Elena. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tandil. Centro de Investigación Veterinaria de Tandil. Universidad Nacional del Centro de la Provincia de Buenos Aires. Centro de Investigación Veterinaria de Tandil. Provincia de Buenos Aires. Gobernación. Comision de Investigaciones Científicas. Centro de Investigación Veterinaria de Tandil; Argentina
54th Annual Meeting Argentine Society for Biochemistry and Molecular Biology
Paraná
Argentina
Sociedad Argentina de Investigación en Bioquímica y Biología Molecular
description Bovine Leukemia Virus (BLV) is a highly prevalent pathogen causing a fatal lymphoproliferative disease in the bovine species. Afterexperimental infection, proviral load peaks at 30 days post infection (dpi), then, cattle progress to two different phenotypes: one is characterizedby high proviral load (HPL) in peripheral blood, while the other is identified by low proviral load (LPL) in peripheral blood. In LPL cattle asharp decrease in proviral load is evidenced at 38 dpi. We studied the transcriptome in peripheral blood cells from 10 cattle (5 of each phenotype)infected with BLV at 38 dpi, to identify the host genes differentially expressed in those animals that progress to LPL, recognized as BLVresistant.RNA seq experiments showed 499 genes differentially expressed (p<0.05) between both phenotypes: 281 upregulated and 218downregulated in LPL compared to HPL cattle. Gene ontology analysis revealed that genes related to inflammatory response, humoral immuneresponse and leukocyte migration were up regulated in cattle progressing to LPL compared to HPL (p< 0.05), while the regulation of homeostaticprocess, antigen receptor signaling pathway and phospholipid transport were downregulated in LPL compared to HPL cattle (p< 0.05). The hugedifference in transcript expression found at 38 dpi, suggests that mechanisms used to control the proviral load are turned on early after theinfection, although the phenotype of LPL or HPL is established only after 90 dpi.
publishDate 2018
dc.date.none.fl_str_mv 2018
dc.type.none.fl_str_mv info:eu-repo/semantics/publishedVersion
info:eu-repo/semantics/conferenceObject
Reunión
Journal
http://purl.org/coar/resource_type/c_5794
info:ar-repo/semantics/documentoDeConferencia
status_str publishedVersion
format conferenceObject
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/233128
Transcriptomic analysis in blv-experimentally infected cattle leading to high or low proviral load; 54th Annual Meeting Argentine Society for Biochemistry and Molecular Biology; Paraná; Argentina; 2018; 1-5
1667-5746
CONICET Digital
CONICET
url http://hdl.handle.net/11336/233128
identifier_str_mv Transcriptomic analysis in blv-experimentally infected cattle leading to high or low proviral load; 54th Annual Meeting Argentine Society for Biochemistry and Molecular Biology; Paraná; Argentina; 2018; 1-5
1667-5746
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/http://www.saib.org.ar/sites/default/files/BIOCELL-SAIB-2018.pdf
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dc.coverage.none.fl_str_mv Internacional
dc.publisher.none.fl_str_mv Tech Science Press
publisher.none.fl_str_mv Tech Science Press
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
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reponame_str CONICET Digital (CONICET)
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repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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