Comparative analysis of production and purification of homo- and hetero-polysaccharides produced by lactic acid bacteria

Autores
Notariago, Sara; Nácher Vázquez, Montserrat; Ibarburu, Idoia; Werning, María Laura; Fernández de Palencia, Pilar; Dueñas, María Teresa; Aznar, Rosa; López, Paloma; Prieto, Alicia
Año de publicación
2013
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Lactic acid bacteria (LAB) produce homopolysaccharides (HoPS) and heteropolysaccharides (HePS) with potential functional properties. In this work, we have performed a comparative analysis of production and purification trials of these biopolymers from bacterial culture supernatants. LAB strains belonging to four different genera, both natural as well as recombinant, were used as model systems for the production of HoPS and HePS. Two well characterized strains carrying the gft gene were used for β-glucan production, Pediococcus parvulus 2.6 (P. parvulus 2.6) isolated from cider, and the recombinant strain Lactococcus lactis NZ9000[pGTF] (L. lactis NZ9000[pGTF]). In addition, another cider isolate, Lactobacillus suebicus CUPV225 (L. suebicus CUPV225), and Leuconostoc mesenteroides RTF10 (L. mesenteroides RTF10), isolated from meat products were included in the study. Chemical analysis of the EPS revealed that L. mesenteroides produces a dextran, L. suebicus a complex heteropolysaccharide, and the β-glucan producing-strains the expected 2-substituted (1,3)-β-glucan.
Fil: Notariago, Sara. Consejo Superior de Investigaciones Científicas. Centro de Investigaciones Biológicas; España
Fil: Nácher Vázquez, Montserrat. Consejo Superior de Investigaciones Científicas. Instituto de Agroquímica y Tecnología de Alimentos; España
Fil: Ibarburu, Idoia. Universidad del País Vasco; España
Fil: Werning, María Laura. Consejo Superior de Investigaciones Científicas. Centro de Investigaciones Biológicas; España. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Fernández de Palencia, Pilar. Consejo Superior de Investigaciones Científicas. Centro de Investigaciones Biologicas; España
Fil: Dueñas, María Teresa. Universidad del País Vasco; España
Fil: Aznar, Rosa. Consejo Superior de Investigaciones Científicas. Instituto de Agroquímica y Tecnología de Alimentos; España. Universidad de Valencia; España
Fil: López, Paloma. Consejo Superior de Investigaciones Científicas. Centro de Investigaciones Biológicas; España
Fil: Prieto, Alicia. Consejo Superior de Investigaciones Científicas. Centro de Investigaciones Biológicas; España
Materia
Lactic Acid Bacteria
Homopolysaccharides
Heteropolysaccharides
Production
Purification
Methods
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/23925
Acceder
id CONICETDig_58fcda4e22f9c74b7ea0e2e56bdc8b4f
oai_identifier_str oai:ri.conicet.gov.ar:11336/23925
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Comparative analysis of production and purification of homo- and hetero-polysaccharides produced by lactic acid bacteriaNotariago, SaraNácher Vázquez, MontserratIbarburu, IdoiaWerning, María LauraFernández de Palencia, PilarDueñas, María TeresaAznar, RosaLópez, PalomaPrieto, AliciaLactic Acid BacteriaHomopolysaccharidesHeteropolysaccharidesProductionPurificationMethodshttps://purl.org/becyt/ford/1.4https://purl.org/becyt/ford/1Lactic acid bacteria (LAB) produce homopolysaccharides (HoPS) and heteropolysaccharides (HePS) with potential functional properties. In this work, we have performed a comparative analysis of production and purification trials of these biopolymers from bacterial culture supernatants. LAB strains belonging to four different genera, both natural as well as recombinant, were used as model systems for the production of HoPS and HePS. Two well characterized strains carrying the gft gene were used for β-glucan production, Pediococcus parvulus 2.6 (P. parvulus 2.6) isolated from cider, and the recombinant strain Lactococcus lactis NZ9000[pGTF] (L. lactis NZ9000[pGTF]). In addition, another cider isolate, Lactobacillus suebicus CUPV225 (L. suebicus CUPV225), and Leuconostoc mesenteroides RTF10 (L. mesenteroides RTF10), isolated from meat products were included in the study. Chemical analysis of the EPS revealed that L. mesenteroides produces a dextran, L. suebicus a complex heteropolysaccharide, and the β-glucan producing-strains the expected 2-substituted (1,3)-β-glucan.Fil: Notariago, Sara. Consejo Superior de Investigaciones Científicas. Centro de Investigaciones Biológicas; EspañaFil: Nácher Vázquez, Montserrat. Consejo Superior de Investigaciones Científicas. Instituto de Agroquímica y Tecnología de Alimentos; EspañaFil: Ibarburu, Idoia. Universidad del País Vasco; EspañaFil: Werning, María Laura. Consejo Superior de Investigaciones Científicas. Centro de Investigaciones Biológicas; España. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Fernández de Palencia, Pilar. Consejo Superior de Investigaciones Científicas. Centro de Investigaciones Biologicas; EspañaFil: Dueñas, María Teresa. Universidad del País Vasco; EspañaFil: Aznar, Rosa. Consejo Superior de Investigaciones Científicas. Instituto de Agroquímica y Tecnología de Alimentos; España. Universidad de Valencia; EspañaFil: López, Paloma. Consejo Superior de Investigaciones Científicas. Centro de Investigaciones Biológicas; EspañaFil: Prieto, Alicia. Consejo Superior de Investigaciones Científicas. Centro de Investigaciones Biológicas; EspañaElsevier2013-03info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/23925Notariago, Sara; Nácher Vázquez, Montserrat; Ibarburu, Idoia; Werning, María Laura; Fernández de Palencia, Pilar; et al.; Comparative analysis of production and purification of homo- and hetero-polysaccharides produced by lactic acid bacteria; Elsevier; Carbohydrate Polymers; 93; 1; 3-2013; 57-640144-8617CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.1016/j.carbpol.2012.05.016info:eu-repo/semantics/altIdentifier/url/http://www.sciencedirect.com/science/article/pii/S0144861712004377info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-03T09:56:31Zoai:ri.conicet.gov.ar:11336/23925instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-03 09:56:32.154CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Comparative analysis of production and purification of homo- and hetero-polysaccharides produced by lactic acid bacteria
title Comparative analysis of production and purification of homo- and hetero-polysaccharides produced by lactic acid bacteria
spellingShingle Comparative analysis of production and purification of homo- and hetero-polysaccharides produced by lactic acid bacteria
Notariago, Sara
Lactic Acid Bacteria
Homopolysaccharides
Heteropolysaccharides
Production
Purification
Methods
title_short Comparative analysis of production and purification of homo- and hetero-polysaccharides produced by lactic acid bacteria
title_full Comparative analysis of production and purification of homo- and hetero-polysaccharides produced by lactic acid bacteria
title_fullStr Comparative analysis of production and purification of homo- and hetero-polysaccharides produced by lactic acid bacteria
title_full_unstemmed Comparative analysis of production and purification of homo- and hetero-polysaccharides produced by lactic acid bacteria
title_sort Comparative analysis of production and purification of homo- and hetero-polysaccharides produced by lactic acid bacteria
dc.creator.none.fl_str_mv Notariago, Sara
Nácher Vázquez, Montserrat
Ibarburu, Idoia
Werning, María Laura
Fernández de Palencia, Pilar
Dueñas, María Teresa
Aznar, Rosa
López, Paloma
Prieto, Alicia
author Notariago, Sara
author_facet Notariago, Sara
Nácher Vázquez, Montserrat
Ibarburu, Idoia
Werning, María Laura
Fernández de Palencia, Pilar
Dueñas, María Teresa
Aznar, Rosa
López, Paloma
Prieto, Alicia
author_role author
author2 Nácher Vázquez, Montserrat
Ibarburu, Idoia
Werning, María Laura
Fernández de Palencia, Pilar
Dueñas, María Teresa
Aznar, Rosa
López, Paloma
Prieto, Alicia
author2_role author
author
author
author
author
author
author
author
dc.subject.none.fl_str_mv Lactic Acid Bacteria
Homopolysaccharides
Heteropolysaccharides
Production
Purification
Methods
topic Lactic Acid Bacteria
Homopolysaccharides
Heteropolysaccharides
Production
Purification
Methods
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.4
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv Lactic acid bacteria (LAB) produce homopolysaccharides (HoPS) and heteropolysaccharides (HePS) with potential functional properties. In this work, we have performed a comparative analysis of production and purification trials of these biopolymers from bacterial culture supernatants. LAB strains belonging to four different genera, both natural as well as recombinant, were used as model systems for the production of HoPS and HePS. Two well characterized strains carrying the gft gene were used for β-glucan production, Pediococcus parvulus 2.6 (P. parvulus 2.6) isolated from cider, and the recombinant strain Lactococcus lactis NZ9000[pGTF] (L. lactis NZ9000[pGTF]). In addition, another cider isolate, Lactobacillus suebicus CUPV225 (L. suebicus CUPV225), and Leuconostoc mesenteroides RTF10 (L. mesenteroides RTF10), isolated from meat products were included in the study. Chemical analysis of the EPS revealed that L. mesenteroides produces a dextran, L. suebicus a complex heteropolysaccharide, and the β-glucan producing-strains the expected 2-substituted (1,3)-β-glucan.
Fil: Notariago, Sara. Consejo Superior de Investigaciones Científicas. Centro de Investigaciones Biológicas; España
Fil: Nácher Vázquez, Montserrat. Consejo Superior de Investigaciones Científicas. Instituto de Agroquímica y Tecnología de Alimentos; España
Fil: Ibarburu, Idoia. Universidad del País Vasco; España
Fil: Werning, María Laura. Consejo Superior de Investigaciones Científicas. Centro de Investigaciones Biológicas; España. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Fernández de Palencia, Pilar. Consejo Superior de Investigaciones Científicas. Centro de Investigaciones Biologicas; España
Fil: Dueñas, María Teresa. Universidad del País Vasco; España
Fil: Aznar, Rosa. Consejo Superior de Investigaciones Científicas. Instituto de Agroquímica y Tecnología de Alimentos; España. Universidad de Valencia; España
Fil: López, Paloma. Consejo Superior de Investigaciones Científicas. Centro de Investigaciones Biológicas; España
Fil: Prieto, Alicia. Consejo Superior de Investigaciones Científicas. Centro de Investigaciones Biológicas; España
description Lactic acid bacteria (LAB) produce homopolysaccharides (HoPS) and heteropolysaccharides (HePS) with potential functional properties. In this work, we have performed a comparative analysis of production and purification trials of these biopolymers from bacterial culture supernatants. LAB strains belonging to four different genera, both natural as well as recombinant, were used as model systems for the production of HoPS and HePS. Two well characterized strains carrying the gft gene were used for β-glucan production, Pediococcus parvulus 2.6 (P. parvulus 2.6) isolated from cider, and the recombinant strain Lactococcus lactis NZ9000[pGTF] (L. lactis NZ9000[pGTF]). In addition, another cider isolate, Lactobacillus suebicus CUPV225 (L. suebicus CUPV225), and Leuconostoc mesenteroides RTF10 (L. mesenteroides RTF10), isolated from meat products were included in the study. Chemical analysis of the EPS revealed that L. mesenteroides produces a dextran, L. suebicus a complex heteropolysaccharide, and the β-glucan producing-strains the expected 2-substituted (1,3)-β-glucan.
publishDate 2013
dc.date.none.fl_str_mv 2013-03
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/23925
Notariago, Sara; Nácher Vázquez, Montserrat; Ibarburu, Idoia; Werning, María Laura; Fernández de Palencia, Pilar; et al.; Comparative analysis of production and purification of homo- and hetero-polysaccharides produced by lactic acid bacteria; Elsevier; Carbohydrate Polymers; 93; 1; 3-2013; 57-64
0144-8617
CONICET Digital
CONICET
url http://hdl.handle.net/11336/23925
identifier_str_mv Notariago, Sara; Nácher Vázquez, Montserrat; Ibarburu, Idoia; Werning, María Laura; Fernández de Palencia, Pilar; et al.; Comparative analysis of production and purification of homo- and hetero-polysaccharides produced by lactic acid bacteria; Elsevier; Carbohydrate Polymers; 93; 1; 3-2013; 57-64
0144-8617
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.1016/j.carbpol.2012.05.016
info:eu-repo/semantics/altIdentifier/url/http://www.sciencedirect.com/science/article/pii/S0144861712004377
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Elsevier
publisher.none.fl_str_mv Elsevier
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
_version_ 1842269407569510400
score 13.13397

Publicaciones similares