Effect of co-administration of lactobacilli S-layer proteins on immune response against S-layer proteins from pathogenic Clostridioides difficile

Autores
Assandri, Matías Hernán; Malamud, Mariano; Valeff, Natalin Jimena; Trejo, Fernando Miguel; Serradell, María de los Ángeles
Año de publicación
2025
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Introduction. Vaccine adjuvants are essential for enhancing and directing specific immune responses. S-layer proteins (SLPs),which coat the surface of many prokaryotes, can induce a variety of responses, including pro- and anti-inflammatory effects.We have previously shown that the SLP from Lentilactobacillus kefiri CIDCA 8348 enhances antigen-specific cellular responses.Hypothesis. The immunostimulatory activity of SLPs from L. kefiri strains could enhance the specific immune response againstthe SLP from the pathogen Clostridioides difficile, which is emerging as an interesting target for vaccine development.Aim. The aim of this study was to evaluate the effect of SLPs from non-pathogenic L. kefiri strains (SLP-Lk) on the immuneresponse against SLPs from pathogenic C. difficile strains (SLP-Cd), using in vitro and in vivo experiments.Methodology. We evaluated the immunostimulatory ability of both SLP-Lk and SLP-Cd, either alone or in combination, on RAW264.7 macrophages by measurement of secreted IL-6 after 24h of stimulation or by determination of cytokine mRNA expression after 4h of incubation with stimuli. Regarding the results, we assessed the impact of SLP-Lk on the levels of anti-SLP-Cdspecific IgG in mouse sera.Results. Among the five SLP-Lk evaluated, only SLP-Lk 8343 and SLP-Lk 83111 increased IL-6 secretion by RAW 264.7 cells,showing a dose–response behaviour, with no stimulation at 2.5µgml−1. In contrast, neither SLP-Cd 117 nor SLP-Cd 43255induced IL-6 secretion by macrophages at 30µgml−1. However, when RAW 264.7 cells were incubated with a mixture of SLP-Lk8343 or SLP-Lk 83111 (at 2.5µg ml−1) and SLP-Cd 117 or SLP-Cd 43255 (at 30µg ml−1), IL-6 secretion increased significantly,irrespective of the SLP-Lk/SLP-Cd pair analysed. Finally, we found that the intraperitoneal co-administration of SLP-Lk 83111resulted in a significant increase in serum levels of anti-SLP-Cd 43255-specific IgG in immunized mice.Conclusion. The ability of SLP-Lk 83111 to enhance the activation of antigen-presenting cells induced by SLP-Cd 43255 correlates with a significant increase in specific antibodies in the sera of mice immunized with SLP-Cd 43255. Further research isrequired to elucidate the roles of these anti-SLP-Cd antibodies in the context of C. difficile infection.
Fil: Assandri, Matías Hernán. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Departamento de Ciencias Biológicas. Cátedra de Microbiología General; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata; Argentina
Fil: Malamud, Mariano. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata; Argentina. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Departamento de Ciencias Biológicas. Cátedra de Microbiología General; Argentina
Fil: Valeff, Natalin Jimena. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Departamento de Ciencias Biológicas. Cátedra de Microbiología General; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Centro de Estudios Farmacológicos y Botánicos. Universidad de Buenos Aires. Facultad de Medicina. Centro de Estudios Farmacológicos y Botánicos; Argentina
Fil: Trejo, Fernando Miguel. Provincia de Buenos Aires. Gobernación. Comisión de Investigaciones Científicas. Centro de Investigación y Desarrollo en Criotecnología de Alimentos. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Centro de Investigación y Desarrollo en Criotecnología de Alimentos. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Centro de Investigación y Desarrollo en Criotecnología de Alimentos; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata; Argentina
Fil: Serradell, María de los Ángeles. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Departamento de Ciencias Biológicas. Cátedra de Microbiología General; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata; Argentina
Materia
Adjuvants
Clostridioides difficile
Lentilactobacillus kefiri
Macrophage activation
S-layer
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/281673
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/281673
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Effect of co-administration of lactobacilli S-layer proteins on immune response against S-layer proteins from pathogenic Clostridioides difficileAssandri, Matías HernánMalamud, MarianoValeff, Natalin JimenaTrejo, Fernando MiguelSerradell, María de los ÁngelesAdjuvantsClostridioides difficileLentilactobacillus kefiriMacrophage activationS-layerhttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1Introduction. Vaccine adjuvants are essential for enhancing and directing specific immune responses. S-layer proteins (SLPs),which coat the surface of many prokaryotes, can induce a variety of responses, including pro- and anti-inflammatory effects.We have previously shown that the SLP from Lentilactobacillus kefiri CIDCA 8348 enhances antigen-specific cellular responses.Hypothesis. The immunostimulatory activity of SLPs from L. kefiri strains could enhance the specific immune response againstthe SLP from the pathogen Clostridioides difficile, which is emerging as an interesting target for vaccine development.Aim. The aim of this study was to evaluate the effect of SLPs from non-pathogenic L. kefiri strains (SLP-Lk) on the immuneresponse against SLPs from pathogenic C. difficile strains (SLP-Cd), using in vitro and in vivo experiments.Methodology. We evaluated the immunostimulatory ability of both SLP-Lk and SLP-Cd, either alone or in combination, on RAW264.7 macrophages by measurement of secreted IL-6 after 24h of stimulation or by determination of cytokine mRNA expression after 4h of incubation with stimuli. Regarding the results, we assessed the impact of SLP-Lk on the levels of anti-SLP-Cdspecific IgG in mouse sera.Results. Among the five SLP-Lk evaluated, only SLP-Lk 8343 and SLP-Lk 83111 increased IL-6 secretion by RAW 264.7 cells,showing a dose–response behaviour, with no stimulation at 2.5µgml−1. In contrast, neither SLP-Cd 117 nor SLP-Cd 43255induced IL-6 secretion by macrophages at 30µgml−1. However, when RAW 264.7 cells were incubated with a mixture of SLP-Lk8343 or SLP-Lk 83111 (at 2.5µg ml−1) and SLP-Cd 117 or SLP-Cd 43255 (at 30µg ml−1), IL-6 secretion increased significantly,irrespective of the SLP-Lk/SLP-Cd pair analysed. Finally, we found that the intraperitoneal co-administration of SLP-Lk 83111resulted in a significant increase in serum levels of anti-SLP-Cd 43255-specific IgG in immunized mice.Conclusion. The ability of SLP-Lk 83111 to enhance the activation of antigen-presenting cells induced by SLP-Cd 43255 correlates with a significant increase in specific antibodies in the sera of mice immunized with SLP-Cd 43255. Further research isrequired to elucidate the roles of these anti-SLP-Cd antibodies in the context of C. difficile infection.Fil: Assandri, Matías Hernán. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Departamento de Ciencias Biológicas. Cátedra de Microbiología General; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata; ArgentinaFil: Malamud, Mariano. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata; Argentina. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Departamento de Ciencias Biológicas. Cátedra de Microbiología General; ArgentinaFil: Valeff, Natalin Jimena. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Departamento de Ciencias Biológicas. Cátedra de Microbiología General; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Centro de Estudios Farmacológicos y Botánicos. Universidad de Buenos Aires. Facultad de Medicina. Centro de Estudios Farmacológicos y Botánicos; ArgentinaFil: Trejo, Fernando Miguel. Provincia de Buenos Aires. Gobernación. Comisión de Investigaciones Científicas. Centro de Investigación y Desarrollo en Criotecnología de Alimentos. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Centro de Investigación y Desarrollo en Criotecnología de Alimentos. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Centro de Investigación y Desarrollo en Criotecnología de Alimentos; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata; ArgentinaFil: Serradell, María de los Ángeles. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Departamento de Ciencias Biológicas. Cátedra de Microbiología General; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata; ArgentinaSociety for General Microbiology2025-09info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/281673Assandri, Matías Hernán; Malamud, Mariano; Valeff, Natalin Jimena; Trejo, Fernando Miguel; Serradell, María de los Ángeles; Effect of co-administration of lactobacilli S-layer proteins on immune response against S-layer proteins from pathogenic Clostridioides difficile; Society for General Microbiology; Journal of Medical Microbiology; 74; 9; 9-2025; 1-110022-2615CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.1099/jmm.0.002068info:eu-repo/semantics/altIdentifier/url/https://pubmed.ncbi.nlm.nih.gov/41004251/info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2026-03-11T12:28:04Zoai:ri.conicet.gov.ar:11336/281673instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982026-03-11 12:28:05.04CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Effect of co-administration of lactobacilli S-layer proteins on immune response against S-layer proteins from pathogenic Clostridioides difficile
title Effect of co-administration of lactobacilli S-layer proteins on immune response against S-layer proteins from pathogenic Clostridioides difficile
spellingShingle Effect of co-administration of lactobacilli S-layer proteins on immune response against S-layer proteins from pathogenic Clostridioides difficile
Assandri, Matías Hernán
Adjuvants
Clostridioides difficile
Lentilactobacillus kefiri
Macrophage activation
S-layer
title_short Effect of co-administration of lactobacilli S-layer proteins on immune response against S-layer proteins from pathogenic Clostridioides difficile
title_full Effect of co-administration of lactobacilli S-layer proteins on immune response against S-layer proteins from pathogenic Clostridioides difficile
title_fullStr Effect of co-administration of lactobacilli S-layer proteins on immune response against S-layer proteins from pathogenic Clostridioides difficile
title_full_unstemmed Effect of co-administration of lactobacilli S-layer proteins on immune response against S-layer proteins from pathogenic Clostridioides difficile
title_sort Effect of co-administration of lactobacilli S-layer proteins on immune response against S-layer proteins from pathogenic Clostridioides difficile
dc.creator.none.fl_str_mv Assandri, Matías Hernán
Malamud, Mariano
Valeff, Natalin Jimena
Trejo, Fernando Miguel
Serradell, María de los Ángeles
author Assandri, Matías Hernán
author_facet Assandri, Matías Hernán
Malamud, Mariano
Valeff, Natalin Jimena
Trejo, Fernando Miguel
Serradell, María de los Ángeles
author_role author
author2 Malamud, Mariano
Valeff, Natalin Jimena
Trejo, Fernando Miguel
Serradell, María de los Ángeles
author2_role author
author
author
author
dc.subject.none.fl_str_mv Adjuvants
Clostridioides difficile
Lentilactobacillus kefiri
Macrophage activation
S-layer
topic Adjuvants
Clostridioides difficile
Lentilactobacillus kefiri
Macrophage activation
S-layer
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv Introduction. Vaccine adjuvants are essential for enhancing and directing specific immune responses. S-layer proteins (SLPs),which coat the surface of many prokaryotes, can induce a variety of responses, including pro- and anti-inflammatory effects.We have previously shown that the SLP from Lentilactobacillus kefiri CIDCA 8348 enhances antigen-specific cellular responses.Hypothesis. The immunostimulatory activity of SLPs from L. kefiri strains could enhance the specific immune response againstthe SLP from the pathogen Clostridioides difficile, which is emerging as an interesting target for vaccine development.Aim. The aim of this study was to evaluate the effect of SLPs from non-pathogenic L. kefiri strains (SLP-Lk) on the immuneresponse against SLPs from pathogenic C. difficile strains (SLP-Cd), using in vitro and in vivo experiments.Methodology. We evaluated the immunostimulatory ability of both SLP-Lk and SLP-Cd, either alone or in combination, on RAW264.7 macrophages by measurement of secreted IL-6 after 24h of stimulation or by determination of cytokine mRNA expression after 4h of incubation with stimuli. Regarding the results, we assessed the impact of SLP-Lk on the levels of anti-SLP-Cdspecific IgG in mouse sera.Results. Among the five SLP-Lk evaluated, only SLP-Lk 8343 and SLP-Lk 83111 increased IL-6 secretion by RAW 264.7 cells,showing a dose–response behaviour, with no stimulation at 2.5µgml−1. In contrast, neither SLP-Cd 117 nor SLP-Cd 43255induced IL-6 secretion by macrophages at 30µgml−1. However, when RAW 264.7 cells were incubated with a mixture of SLP-Lk8343 or SLP-Lk 83111 (at 2.5µg ml−1) and SLP-Cd 117 or SLP-Cd 43255 (at 30µg ml−1), IL-6 secretion increased significantly,irrespective of the SLP-Lk/SLP-Cd pair analysed. Finally, we found that the intraperitoneal co-administration of SLP-Lk 83111resulted in a significant increase in serum levels of anti-SLP-Cd 43255-specific IgG in immunized mice.Conclusion. The ability of SLP-Lk 83111 to enhance the activation of antigen-presenting cells induced by SLP-Cd 43255 correlates with a significant increase in specific antibodies in the sera of mice immunized with SLP-Cd 43255. Further research isrequired to elucidate the roles of these anti-SLP-Cd antibodies in the context of C. difficile infection.
Fil: Assandri, Matías Hernán. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Departamento de Ciencias Biológicas. Cátedra de Microbiología General; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata; Argentina
Fil: Malamud, Mariano. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata; Argentina. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Departamento de Ciencias Biológicas. Cátedra de Microbiología General; Argentina
Fil: Valeff, Natalin Jimena. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Departamento de Ciencias Biológicas. Cátedra de Microbiología General; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Centro de Estudios Farmacológicos y Botánicos. Universidad de Buenos Aires. Facultad de Medicina. Centro de Estudios Farmacológicos y Botánicos; Argentina
Fil: Trejo, Fernando Miguel. Provincia de Buenos Aires. Gobernación. Comisión de Investigaciones Científicas. Centro de Investigación y Desarrollo en Criotecnología de Alimentos. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Centro de Investigación y Desarrollo en Criotecnología de Alimentos. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Centro de Investigación y Desarrollo en Criotecnología de Alimentos; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata; Argentina
Fil: Serradell, María de los Ángeles. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Departamento de Ciencias Biológicas. Cátedra de Microbiología General; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata; Argentina
description Introduction. Vaccine adjuvants are essential for enhancing and directing specific immune responses. S-layer proteins (SLPs),which coat the surface of many prokaryotes, can induce a variety of responses, including pro- and anti-inflammatory effects.We have previously shown that the SLP from Lentilactobacillus kefiri CIDCA 8348 enhances antigen-specific cellular responses.Hypothesis. The immunostimulatory activity of SLPs from L. kefiri strains could enhance the specific immune response againstthe SLP from the pathogen Clostridioides difficile, which is emerging as an interesting target for vaccine development.Aim. The aim of this study was to evaluate the effect of SLPs from non-pathogenic L. kefiri strains (SLP-Lk) on the immuneresponse against SLPs from pathogenic C. difficile strains (SLP-Cd), using in vitro and in vivo experiments.Methodology. We evaluated the immunostimulatory ability of both SLP-Lk and SLP-Cd, either alone or in combination, on RAW264.7 macrophages by measurement of secreted IL-6 after 24h of stimulation or by determination of cytokine mRNA expression after 4h of incubation with stimuli. Regarding the results, we assessed the impact of SLP-Lk on the levels of anti-SLP-Cdspecific IgG in mouse sera.Results. Among the five SLP-Lk evaluated, only SLP-Lk 8343 and SLP-Lk 83111 increased IL-6 secretion by RAW 264.7 cells,showing a dose–response behaviour, with no stimulation at 2.5µgml−1. In contrast, neither SLP-Cd 117 nor SLP-Cd 43255induced IL-6 secretion by macrophages at 30µgml−1. However, when RAW 264.7 cells were incubated with a mixture of SLP-Lk8343 or SLP-Lk 83111 (at 2.5µg ml−1) and SLP-Cd 117 or SLP-Cd 43255 (at 30µg ml−1), IL-6 secretion increased significantly,irrespective of the SLP-Lk/SLP-Cd pair analysed. Finally, we found that the intraperitoneal co-administration of SLP-Lk 83111resulted in a significant increase in serum levels of anti-SLP-Cd 43255-specific IgG in immunized mice.Conclusion. The ability of SLP-Lk 83111 to enhance the activation of antigen-presenting cells induced by SLP-Cd 43255 correlates with a significant increase in specific antibodies in the sera of mice immunized with SLP-Cd 43255. Further research isrequired to elucidate the roles of these anti-SLP-Cd antibodies in the context of C. difficile infection.
publishDate 2025
dc.date.none.fl_str_mv 2025-09
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/281673
Assandri, Matías Hernán; Malamud, Mariano; Valeff, Natalin Jimena; Trejo, Fernando Miguel; Serradell, María de los Ángeles; Effect of co-administration of lactobacilli S-layer proteins on immune response against S-layer proteins from pathogenic Clostridioides difficile; Society for General Microbiology; Journal of Medical Microbiology; 74; 9; 9-2025; 1-11
0022-2615
CONICET Digital
CONICET
url http://hdl.handle.net/11336/281673
identifier_str_mv Assandri, Matías Hernán; Malamud, Mariano; Valeff, Natalin Jimena; Trejo, Fernando Miguel; Serradell, María de los Ángeles; Effect of co-administration of lactobacilli S-layer proteins on immune response against S-layer proteins from pathogenic Clostridioides difficile; Society for General Microbiology; Journal of Medical Microbiology; 74; 9; 9-2025; 1-11
0022-2615
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.1099/jmm.0.002068
info:eu-repo/semantics/altIdentifier/url/https://pubmed.ncbi.nlm.nih.gov/41004251/
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Society for General Microbiology
publisher.none.fl_str_mv Society for General Microbiology
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 12.977003

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