An Approach to the Effects of Chlorpyrifos on Jenynsia lineata Exposed to Environmentally Relevant Concentrations: Biomarkers of Oxidative Stress, Neurotoxicity and Genotoxicity
- Autores
- Lombardero, Lucas Rodrigo; Medici, Sandra Karina; Mendieta, Julieta Renee; Villagran, Diana Mariel; Pérez, Débora Jesabel; Menone, Mirta Lujan
- Año de publicación
- 2023
- Idioma
- inglés
- Tipo de recurso
- documento de conferencia
- Estado
- versión publicada
- Descripción
- Chlorpyrifos (CPF) is a common organophosphate insecticide used for pest control in agriculture and nonagricultural settings, acting as an acetylcholinesterase (AChE) inhibitor. It is highly toxic to aquatic life and can persist in the environment (half- life: 81 days in surface water). The aim of the present study was to evaluate effects of oxidative stress and damage, neurotoxicity and genotoxicity in Jenynsia lineata exposed to environmentally relevant concentrations of CPF during 96 h. Five tanks (30 L) were set up, using females of J. lineata (n=6 per treatment; standard length: 4.31 ± 0.50 cm; total weight: 1.67 ± 0.51 gr), one as a control and four with CPF concentrations of 0.05, 0.5, 5, and 50 μg/L. Liver, gills, and brain were dissected and immediately frozen with liquid nitrogen and stored at -80°C until biomarkers analysis. The blood was extracted and smear were done and fixed with methanol for further analysis. During the course of the experiment, three individuals exposed at 50 ug/L and one exposed at 5 ug/L died at the first 24 hs indicating that this concentration is closed to the LC50. While the remaining three individuals exposed at 5o ug/L wereeuthanized because they looked moribund, and were used for measuring biomarkers. An increase in the activity of the enzymes catalase and glutathione-S-transferase in brain, and of superoxide dismutase in gills was observed at 0.05 μg/L (p<0.05). There were no significant differences in the activity of AChE between the control and the lowest concentration (0.05 μg/L) (p>0.05), while inhibition of AChE was observed at 0.5 μg/L (29.51%) and 5 μg/L (49.53%) (p<0.05). No significant differences were observed for micronuclei or other nuclear aberrations (p>0.05). At 50 μg/L, increased activity of CAT in liver and a 77.6% inhibition of AChE was observed (p<0.05). The results of this study demonstrate that environmentally concentrations of CPF can induce oxidative stress at concentrations lower than those at which AChE inhibition occurs. Although AChE is the target biomarker for organophosphates, it may not be as efficient early warning system as antioxidant enzymes, highlighting the approach of not always use the mode of action biomarkers. Taking into account that CPF values of the lethal concentration 50 (LC50) for other fish species are very high compared to the concentrations used in this experiment, next steps will include the evaluationof the LC50 in J. lineata.
Fil: Lombardero, Lucas Rodrigo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mar del Plata. Instituto de Investigaciones Marinas y Costeras. Universidad Nacional de Mar del Plata. Facultad de Ciencias Exactas y Naturales. Instituto de Investigaciones Marinas y Costeras; Argentina
Fil: Medici, Sandra Karina. Universidad Nacional de Mar del Plata. Instituto de Investigaciones En Produccion, Sanidad y Ambiente. - Consejo Nacional de Investigaciones Cientificas y Tecnicas. Centro Cientifico Tecnologico Conicet - Mar del Plata. Instituto de Investigaciones En Produccion, Sanidad y Ambiente.; Argentina
Fil: Mendieta, Julieta Renee. Provincia de Buenos Aires. Gobernación. Comisión de Investigaciones Científicas; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mar del Plata. Instituto de Investigaciones Biológicas. Universidad Nacional de Mar del Plata. Facultad de Ciencias Exactas y Naturales. Instituto de Investigaciones Biológicas; Argentina
Fil: Villagran, Diana Mariel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mar del Plata. Instituto de Investigaciones Marinas y Costeras. Universidad Nacional de Mar del Plata. Facultad de Ciencias Exactas y Naturales. Instituto de Investigaciones Marinas y Costeras; Argentina
Fil: Pérez, Débora Jesabel. Consejo Nacional de Investigaciones Cientificas y Tecnicas. Centro Cientifico Tecnologico Conicet - Mar del Plata. Instituto de Innovación Para la Producción Agropecuaria y El Desarrollo Sostenible. - Instituto Nacional de Tecnologia Agropecuaria. Centro Regional Buenos Aires Sur. Estacion Experimental Agropecuaria Balcarce. Instituto de Innovación Para la Producción Agropecuaria y El Desarrollo Sostenible.; Argentina
Fil: Menone, Mirta Lujan. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mar del Plata. Instituto de Investigaciones Marinas y Costeras. Universidad Nacional de Mar del Plata. Facultad de Ciencias Exactas y Naturales. Instituto de Investigaciones Marinas y Costeras; Argentina
SETAC Latin America 15th Biennial Meeting
Montevideo
Uruguay
Society of Environmental Toxicology and Chemistry - Materia
-
JENYNSIA LINEATA
CHLORPYRYFOS
SUBLETHAL EFFECTS
ENVIRONMENTAL RELEVANT CONCENTRATIONS - Nivel de accesibilidad
- acceso abierto
- Condiciones de uso
- https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
- Repositorio
.jpg)
- Institución
- Consejo Nacional de Investigaciones Científicas y Técnicas
- OAI Identificador
- oai:ri.conicet.gov.ar:11336/272044
Ver los metadatos del registro completo
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An Approach to the Effects of Chlorpyrifos on Jenynsia lineata Exposed to Environmentally Relevant Concentrations: Biomarkers of Oxidative Stress, Neurotoxicity and GenotoxicityLombardero, Lucas RodrigoMedici, Sandra KarinaMendieta, Julieta ReneeVillagran, Diana MarielPérez, Débora JesabelMenone, Mirta LujanJENYNSIA LINEATACHLORPYRYFOSSUBLETHAL EFFECTSENVIRONMENTAL RELEVANT CONCENTRATIONShttps://purl.org/becyt/ford/1.5https://purl.org/becyt/ford/1Chlorpyrifos (CPF) is a common organophosphate insecticide used for pest control in agriculture and nonagricultural settings, acting as an acetylcholinesterase (AChE) inhibitor. It is highly toxic to aquatic life and can persist in the environment (half- life: 81 days in surface water). The aim of the present study was to evaluate effects of oxidative stress and damage, neurotoxicity and genotoxicity in Jenynsia lineata exposed to environmentally relevant concentrations of CPF during 96 h. Five tanks (30 L) were set up, using females of J. lineata (n=6 per treatment; standard length: 4.31 ± 0.50 cm; total weight: 1.67 ± 0.51 gr), one as a control and four with CPF concentrations of 0.05, 0.5, 5, and 50 μg/L. Liver, gills, and brain were dissected and immediately frozen with liquid nitrogen and stored at -80°C until biomarkers analysis. The blood was extracted and smear were done and fixed with methanol for further analysis. During the course of the experiment, three individuals exposed at 50 ug/L and one exposed at 5 ug/L died at the first 24 hs indicating that this concentration is closed to the LC50. While the remaining three individuals exposed at 5o ug/L wereeuthanized because they looked moribund, and were used for measuring biomarkers. An increase in the activity of the enzymes catalase and glutathione-S-transferase in brain, and of superoxide dismutase in gills was observed at 0.05 μg/L (p<0.05). There were no significant differences in the activity of AChE between the control and the lowest concentration (0.05 μg/L) (p>0.05), while inhibition of AChE was observed at 0.5 μg/L (29.51%) and 5 μg/L (49.53%) (p<0.05). No significant differences were observed for micronuclei or other nuclear aberrations (p>0.05). At 50 μg/L, increased activity of CAT in liver and a 77.6% inhibition of AChE was observed (p<0.05). The results of this study demonstrate that environmentally concentrations of CPF can induce oxidative stress at concentrations lower than those at which AChE inhibition occurs. Although AChE is the target biomarker for organophosphates, it may not be as efficient early warning system as antioxidant enzymes, highlighting the approach of not always use the mode of action biomarkers. Taking into account that CPF values of the lethal concentration 50 (LC50) for other fish species are very high compared to the concentrations used in this experiment, next steps will include the evaluationof the LC50 in J. lineata.Fil: Lombardero, Lucas Rodrigo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mar del Plata. Instituto de Investigaciones Marinas y Costeras. Universidad Nacional de Mar del Plata. Facultad de Ciencias Exactas y Naturales. Instituto de Investigaciones Marinas y Costeras; ArgentinaFil: Medici, Sandra Karina. Universidad Nacional de Mar del Plata. Instituto de Investigaciones En Produccion, Sanidad y Ambiente. - Consejo Nacional de Investigaciones Cientificas y Tecnicas. Centro Cientifico Tecnologico Conicet - Mar del Plata. Instituto de Investigaciones En Produccion, Sanidad y Ambiente.; ArgentinaFil: Mendieta, Julieta Renee. Provincia de Buenos Aires. Gobernación. Comisión de Investigaciones Científicas; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mar del Plata. Instituto de Investigaciones Biológicas. Universidad Nacional de Mar del Plata. Facultad de Ciencias Exactas y Naturales. Instituto de Investigaciones Biológicas; ArgentinaFil: Villagran, Diana Mariel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mar del Plata. Instituto de Investigaciones Marinas y Costeras. Universidad Nacional de Mar del Plata. Facultad de Ciencias Exactas y Naturales. Instituto de Investigaciones Marinas y Costeras; ArgentinaFil: Pérez, Débora Jesabel. Consejo Nacional de Investigaciones Cientificas y Tecnicas. Centro Cientifico Tecnologico Conicet - Mar del Plata. Instituto de Innovación Para la Producción Agropecuaria y El Desarrollo Sostenible. - Instituto Nacional de Tecnologia Agropecuaria. Centro Regional Buenos Aires Sur. Estacion Experimental Agropecuaria Balcarce. Instituto de Innovación Para la Producción Agropecuaria y El Desarrollo Sostenible.; ArgentinaFil: Menone, Mirta Lujan. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mar del Plata. Instituto de Investigaciones Marinas y Costeras. Universidad Nacional de Mar del Plata. Facultad de Ciencias Exactas y Naturales. Instituto de Investigaciones Marinas y Costeras; ArgentinaSETAC Latin America 15th Biennial MeetingMontevideoUruguaySociety of Environmental Toxicology and ChemistrySociety of Environmental Toxicology and Chemistry2023info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/conferenceObjectCongresoBookhttp://purl.org/coar/resource_type/c_5794info:ar-repo/semantics/documentoDeConferenciaapplication/pdfapplication/pdfapplication/pdfapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/272044An Approach to the Effects of Chlorpyrifos on Jenynsia lineata Exposed to Environmentally Relevant Concentrations: Biomarkers of Oxidative Stress, Neurotoxicity and Genotoxicity; SETAC Latin America 15th Biennial Meeting; Montevideo; Uruguay; 2023; 406-406CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/pmid/https://www.setac.org/discover-events/global-meetings/sla-15th-biennial-meeting.htmlInternacionalinfo:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T09:47:14Zoai:ri.conicet.gov.ar:11336/272044instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 09:47:14.403CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse |
| dc.title.none.fl_str_mv |
An Approach to the Effects of Chlorpyrifos on Jenynsia lineata Exposed to Environmentally Relevant Concentrations: Biomarkers of Oxidative Stress, Neurotoxicity and Genotoxicity |
| title |
An Approach to the Effects of Chlorpyrifos on Jenynsia lineata Exposed to Environmentally Relevant Concentrations: Biomarkers of Oxidative Stress, Neurotoxicity and Genotoxicity |
| spellingShingle |
An Approach to the Effects of Chlorpyrifos on Jenynsia lineata Exposed to Environmentally Relevant Concentrations: Biomarkers of Oxidative Stress, Neurotoxicity and Genotoxicity Lombardero, Lucas Rodrigo JENYNSIA LINEATA CHLORPYRYFOS SUBLETHAL EFFECTS ENVIRONMENTAL RELEVANT CONCENTRATIONS |
| title_short |
An Approach to the Effects of Chlorpyrifos on Jenynsia lineata Exposed to Environmentally Relevant Concentrations: Biomarkers of Oxidative Stress, Neurotoxicity and Genotoxicity |
| title_full |
An Approach to the Effects of Chlorpyrifos on Jenynsia lineata Exposed to Environmentally Relevant Concentrations: Biomarkers of Oxidative Stress, Neurotoxicity and Genotoxicity |
| title_fullStr |
An Approach to the Effects of Chlorpyrifos on Jenynsia lineata Exposed to Environmentally Relevant Concentrations: Biomarkers of Oxidative Stress, Neurotoxicity and Genotoxicity |
| title_full_unstemmed |
An Approach to the Effects of Chlorpyrifos on Jenynsia lineata Exposed to Environmentally Relevant Concentrations: Biomarkers of Oxidative Stress, Neurotoxicity and Genotoxicity |
| title_sort |
An Approach to the Effects of Chlorpyrifos on Jenynsia lineata Exposed to Environmentally Relevant Concentrations: Biomarkers of Oxidative Stress, Neurotoxicity and Genotoxicity |
| dc.creator.none.fl_str_mv |
Lombardero, Lucas Rodrigo Medici, Sandra Karina Mendieta, Julieta Renee Villagran, Diana Mariel Pérez, Débora Jesabel Menone, Mirta Lujan |
| author |
Lombardero, Lucas Rodrigo |
| author_facet |
Lombardero, Lucas Rodrigo Medici, Sandra Karina Mendieta, Julieta Renee Villagran, Diana Mariel Pérez, Débora Jesabel Menone, Mirta Lujan |
| author_role |
author |
| author2 |
Medici, Sandra Karina Mendieta, Julieta Renee Villagran, Diana Mariel Pérez, Débora Jesabel Menone, Mirta Lujan |
| author2_role |
author author author author author |
| dc.subject.none.fl_str_mv |
JENYNSIA LINEATA CHLORPYRYFOS SUBLETHAL EFFECTS ENVIRONMENTAL RELEVANT CONCENTRATIONS |
| topic |
JENYNSIA LINEATA CHLORPYRYFOS SUBLETHAL EFFECTS ENVIRONMENTAL RELEVANT CONCENTRATIONS |
| purl_subject.fl_str_mv |
https://purl.org/becyt/ford/1.5 https://purl.org/becyt/ford/1 |
| dc.description.none.fl_txt_mv |
Chlorpyrifos (CPF) is a common organophosphate insecticide used for pest control in agriculture and nonagricultural settings, acting as an acetylcholinesterase (AChE) inhibitor. It is highly toxic to aquatic life and can persist in the environment (half- life: 81 days in surface water). The aim of the present study was to evaluate effects of oxidative stress and damage, neurotoxicity and genotoxicity in Jenynsia lineata exposed to environmentally relevant concentrations of CPF during 96 h. Five tanks (30 L) were set up, using females of J. lineata (n=6 per treatment; standard length: 4.31 ± 0.50 cm; total weight: 1.67 ± 0.51 gr), one as a control and four with CPF concentrations of 0.05, 0.5, 5, and 50 μg/L. Liver, gills, and brain were dissected and immediately frozen with liquid nitrogen and stored at -80°C until biomarkers analysis. The blood was extracted and smear were done and fixed with methanol for further analysis. During the course of the experiment, three individuals exposed at 50 ug/L and one exposed at 5 ug/L died at the first 24 hs indicating that this concentration is closed to the LC50. While the remaining three individuals exposed at 5o ug/L wereeuthanized because they looked moribund, and were used for measuring biomarkers. An increase in the activity of the enzymes catalase and glutathione-S-transferase in brain, and of superoxide dismutase in gills was observed at 0.05 μg/L (p<0.05). There were no significant differences in the activity of AChE between the control and the lowest concentration (0.05 μg/L) (p>0.05), while inhibition of AChE was observed at 0.5 μg/L (29.51%) and 5 μg/L (49.53%) (p<0.05). No significant differences were observed for micronuclei or other nuclear aberrations (p>0.05). At 50 μg/L, increased activity of CAT in liver and a 77.6% inhibition of AChE was observed (p<0.05). The results of this study demonstrate that environmentally concentrations of CPF can induce oxidative stress at concentrations lower than those at which AChE inhibition occurs. Although AChE is the target biomarker for organophosphates, it may not be as efficient early warning system as antioxidant enzymes, highlighting the approach of not always use the mode of action biomarkers. Taking into account that CPF values of the lethal concentration 50 (LC50) for other fish species are very high compared to the concentrations used in this experiment, next steps will include the evaluationof the LC50 in J. lineata. Fil: Lombardero, Lucas Rodrigo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mar del Plata. Instituto de Investigaciones Marinas y Costeras. Universidad Nacional de Mar del Plata. Facultad de Ciencias Exactas y Naturales. Instituto de Investigaciones Marinas y Costeras; Argentina Fil: Medici, Sandra Karina. Universidad Nacional de Mar del Plata. Instituto de Investigaciones En Produccion, Sanidad y Ambiente. - Consejo Nacional de Investigaciones Cientificas y Tecnicas. Centro Cientifico Tecnologico Conicet - Mar del Plata. Instituto de Investigaciones En Produccion, Sanidad y Ambiente.; Argentina Fil: Mendieta, Julieta Renee. Provincia de Buenos Aires. Gobernación. Comisión de Investigaciones Científicas; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mar del Plata. Instituto de Investigaciones Biológicas. Universidad Nacional de Mar del Plata. Facultad de Ciencias Exactas y Naturales. Instituto de Investigaciones Biológicas; Argentina Fil: Villagran, Diana Mariel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mar del Plata. Instituto de Investigaciones Marinas y Costeras. Universidad Nacional de Mar del Plata. Facultad de Ciencias Exactas y Naturales. Instituto de Investigaciones Marinas y Costeras; Argentina Fil: Pérez, Débora Jesabel. Consejo Nacional de Investigaciones Cientificas y Tecnicas. Centro Cientifico Tecnologico Conicet - Mar del Plata. Instituto de Innovación Para la Producción Agropecuaria y El Desarrollo Sostenible. - Instituto Nacional de Tecnologia Agropecuaria. Centro Regional Buenos Aires Sur. Estacion Experimental Agropecuaria Balcarce. Instituto de Innovación Para la Producción Agropecuaria y El Desarrollo Sostenible.; Argentina Fil: Menone, Mirta Lujan. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mar del Plata. Instituto de Investigaciones Marinas y Costeras. Universidad Nacional de Mar del Plata. Facultad de Ciencias Exactas y Naturales. Instituto de Investigaciones Marinas y Costeras; Argentina SETAC Latin America 15th Biennial Meeting Montevideo Uruguay Society of Environmental Toxicology and Chemistry |
| description |
Chlorpyrifos (CPF) is a common organophosphate insecticide used for pest control in agriculture and nonagricultural settings, acting as an acetylcholinesterase (AChE) inhibitor. It is highly toxic to aquatic life and can persist in the environment (half- life: 81 days in surface water). The aim of the present study was to evaluate effects of oxidative stress and damage, neurotoxicity and genotoxicity in Jenynsia lineata exposed to environmentally relevant concentrations of CPF during 96 h. Five tanks (30 L) were set up, using females of J. lineata (n=6 per treatment; standard length: 4.31 ± 0.50 cm; total weight: 1.67 ± 0.51 gr), one as a control and four with CPF concentrations of 0.05, 0.5, 5, and 50 μg/L. Liver, gills, and brain were dissected and immediately frozen with liquid nitrogen and stored at -80°C until biomarkers analysis. The blood was extracted and smear were done and fixed with methanol for further analysis. During the course of the experiment, three individuals exposed at 50 ug/L and one exposed at 5 ug/L died at the first 24 hs indicating that this concentration is closed to the LC50. While the remaining three individuals exposed at 5o ug/L wereeuthanized because they looked moribund, and were used for measuring biomarkers. An increase in the activity of the enzymes catalase and glutathione-S-transferase in brain, and of superoxide dismutase in gills was observed at 0.05 μg/L (p<0.05). There were no significant differences in the activity of AChE between the control and the lowest concentration (0.05 μg/L) (p>0.05), while inhibition of AChE was observed at 0.5 μg/L (29.51%) and 5 μg/L (49.53%) (p<0.05). No significant differences were observed for micronuclei or other nuclear aberrations (p>0.05). At 50 μg/L, increased activity of CAT in liver and a 77.6% inhibition of AChE was observed (p<0.05). The results of this study demonstrate that environmentally concentrations of CPF can induce oxidative stress at concentrations lower than those at which AChE inhibition occurs. Although AChE is the target biomarker for organophosphates, it may not be as efficient early warning system as antioxidant enzymes, highlighting the approach of not always use the mode of action biomarkers. Taking into account that CPF values of the lethal concentration 50 (LC50) for other fish species are very high compared to the concentrations used in this experiment, next steps will include the evaluationof the LC50 in J. lineata. |
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2023 |
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2023 |
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An Approach to the Effects of Chlorpyrifos on Jenynsia lineata Exposed to Environmentally Relevant Concentrations: Biomarkers of Oxidative Stress, Neurotoxicity and Genotoxicity; SETAC Latin America 15th Biennial Meeting; Montevideo; Uruguay; 2023; 406-406 CONICET Digital CONICET |
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eng |
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