Characterization of the mouse promoter region of the acyl-CoA synthetase 4 gene: Role of Sp1 and CREB

Autores
Orlando, Ulises Daniel; Cooke, Mariana; Cornejo Maciel, Fabiana; Papadopoulos, Vassilios; Podesta, Ernesto Jorge; Maloberti, Paula Mariana
Año de publicación
2013
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Acyl-CoA synthetase 4 (Acsl4) is involved in several cellular functions including steroidogenesis, synaptic development and cancer metastasis. Although the expression of Acsl4 seems to be regulated by tissue- and cell-specific factors as well as pituitary hormones and growth factors, the transcriptional mechanisms involved remain unknown. We demonstrated hCG and cAMP regulation of Acsl4 mRNA in mouse steroidogenic MA-10 Leydig cells. We characterized the transcription initiation site and promoter of the Acsl4 mouse gene and identified three alternative splice variants present in MA-10 cells. Sequence analysis of a 1.5-kb fragment of the Acsl4 promoter revealed the absence of a TATA box and the presence of many putative binding sites for transcription factors including Sp1 and CREB. Functional characterization revealed that the specificity protein/Krüppel-like factor Sp1 binding site in the proximal promoter is involved in basal activity and that the cAMP response element-binding site is involved in cAMP stimulation of Acsl4 transcription.
Fil: Orlando, Ulises Daniel. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Investigaciones Biomédicas; Argentina
Fil: Cooke, Mariana. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Investigaciones Biomédicas; Argentina
Fil: Cornejo Maciel, Fabiana. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Investigaciones Biomédicas; Argentina
Fil: Papadopoulos, Vassilios. Mc Gill University; Canadá
Fil: Podesta, Ernesto Jorge. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Investigaciones Biomédicas; Argentina
Fil: Maloberti, Paula Mariana. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Investigaciones Biomédicas; Argentina
Materia
Acss
Transcription Factors
Arachidonic Acid
Steroidogenesis
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/8523
Acceder
id CONICETDig_57a99b0c9e74aea00bbfdab240bfe0e8
oai_identifier_str oai:ri.conicet.gov.ar:11336/8523
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Characterization of the mouse promoter region of the acyl-CoA synthetase 4 gene: Role of Sp1 and CREBOrlando, Ulises DanielCooke, MarianaCornejo Maciel, FabianaPapadopoulos, VassiliosPodesta, Ernesto JorgeMaloberti, Paula MarianaAcssTranscription FactorsArachidonic AcidSteroidogenesishttps://purl.org/becyt/ford/3.1https://purl.org/becyt/ford/3Acyl-CoA synthetase 4 (Acsl4) is involved in several cellular functions including steroidogenesis, synaptic development and cancer metastasis. Although the expression of Acsl4 seems to be regulated by tissue- and cell-specific factors as well as pituitary hormones and growth factors, the transcriptional mechanisms involved remain unknown. We demonstrated hCG and cAMP regulation of Acsl4 mRNA in mouse steroidogenic MA-10 Leydig cells. We characterized the transcription initiation site and promoter of the Acsl4 mouse gene and identified three alternative splice variants present in MA-10 cells. Sequence analysis of a 1.5-kb fragment of the Acsl4 promoter revealed the absence of a TATA box and the presence of many putative binding sites for transcription factors including Sp1 and CREB. Functional characterization revealed that the specificity protein/Krüppel-like factor Sp1 binding site in the proximal promoter is involved in basal activity and that the cAMP response element-binding site is involved in cAMP stimulation of Acsl4 transcription.Fil: Orlando, Ulises Daniel. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Investigaciones Biomédicas; ArgentinaFil: Cooke, Mariana. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Investigaciones Biomédicas; ArgentinaFil: Cornejo Maciel, Fabiana. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Investigaciones Biomédicas; ArgentinaFil: Papadopoulos, Vassilios. Mc Gill University; CanadáFil: Podesta, Ernesto Jorge. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Investigaciones Biomédicas; ArgentinaFil: Maloberti, Paula Mariana. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Investigaciones Biomédicas; ArgentinaElsevier Ireland2013-04info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/8523Orlando, Ulises Daniel; Cooke, Mariana; Cornejo Maciel, Fabiana; Papadopoulos, Vassilios; Podesta, Ernesto Jorge; et al.; Characterization of the mouse promoter region of the acyl-CoA synthetase 4 gene: Role of Sp1 and CREB ; Elsevier Ireland; Molecular And Cellular Endocrinology; 369; 1-2; 4-2013; 15-260303-7207enginfo:eu-repo/semantics/altIdentifier/url/http://www.sciencedirect.com/science/article/pii/S0303720713000397info:eu-repo/semantics/altIdentifier/doi/10.1016/j.mce.2013.01.016info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-nd/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-10-22T11:46:19Zoai:ri.conicet.gov.ar:11336/8523instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-10-22 11:46:20.123CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Characterization of the mouse promoter region of the acyl-CoA synthetase 4 gene: Role of Sp1 and CREB
title Characterization of the mouse promoter region of the acyl-CoA synthetase 4 gene: Role of Sp1 and CREB
spellingShingle Characterization of the mouse promoter region of the acyl-CoA synthetase 4 gene: Role of Sp1 and CREB
Orlando, Ulises Daniel
Acss
Transcription Factors
Arachidonic Acid
Steroidogenesis
title_short Characterization of the mouse promoter region of the acyl-CoA synthetase 4 gene: Role of Sp1 and CREB
title_full Characterization of the mouse promoter region of the acyl-CoA synthetase 4 gene: Role of Sp1 and CREB
title_fullStr Characterization of the mouse promoter region of the acyl-CoA synthetase 4 gene: Role of Sp1 and CREB
title_full_unstemmed Characterization of the mouse promoter region of the acyl-CoA synthetase 4 gene: Role of Sp1 and CREB
title_sort Characterization of the mouse promoter region of the acyl-CoA synthetase 4 gene: Role of Sp1 and CREB
dc.creator.none.fl_str_mv Orlando, Ulises Daniel
Cooke, Mariana
Cornejo Maciel, Fabiana
Papadopoulos, Vassilios
Podesta, Ernesto Jorge
Maloberti, Paula Mariana
author Orlando, Ulises Daniel
author_facet Orlando, Ulises Daniel
Cooke, Mariana
Cornejo Maciel, Fabiana
Papadopoulos, Vassilios
Podesta, Ernesto Jorge
Maloberti, Paula Mariana
author_role author
author2 Cooke, Mariana
Cornejo Maciel, Fabiana
Papadopoulos, Vassilios
Podesta, Ernesto Jorge
Maloberti, Paula Mariana
author2_role author
author
author
author
author
dc.subject.none.fl_str_mv Acss
Transcription Factors
Arachidonic Acid
Steroidogenesis
topic Acss
Transcription Factors
Arachidonic Acid
Steroidogenesis
purl_subject.fl_str_mv https://purl.org/becyt/ford/3.1
https://purl.org/becyt/ford/3
dc.description.none.fl_txt_mv Acyl-CoA synthetase 4 (Acsl4) is involved in several cellular functions including steroidogenesis, synaptic development and cancer metastasis. Although the expression of Acsl4 seems to be regulated by tissue- and cell-specific factors as well as pituitary hormones and growth factors, the transcriptional mechanisms involved remain unknown. We demonstrated hCG and cAMP regulation of Acsl4 mRNA in mouse steroidogenic MA-10 Leydig cells. We characterized the transcription initiation site and promoter of the Acsl4 mouse gene and identified three alternative splice variants present in MA-10 cells. Sequence analysis of a 1.5-kb fragment of the Acsl4 promoter revealed the absence of a TATA box and the presence of many putative binding sites for transcription factors including Sp1 and CREB. Functional characterization revealed that the specificity protein/Krüppel-like factor Sp1 binding site in the proximal promoter is involved in basal activity and that the cAMP response element-binding site is involved in cAMP stimulation of Acsl4 transcription.
Fil: Orlando, Ulises Daniel. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Investigaciones Biomédicas; Argentina
Fil: Cooke, Mariana. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Investigaciones Biomédicas; Argentina
Fil: Cornejo Maciel, Fabiana. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Investigaciones Biomédicas; Argentina
Fil: Papadopoulos, Vassilios. Mc Gill University; Canadá
Fil: Podesta, Ernesto Jorge. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Investigaciones Biomédicas; Argentina
Fil: Maloberti, Paula Mariana. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Investigaciones Biomédicas; Argentina
description Acyl-CoA synthetase 4 (Acsl4) is involved in several cellular functions including steroidogenesis, synaptic development and cancer metastasis. Although the expression of Acsl4 seems to be regulated by tissue- and cell-specific factors as well as pituitary hormones and growth factors, the transcriptional mechanisms involved remain unknown. We demonstrated hCG and cAMP regulation of Acsl4 mRNA in mouse steroidogenic MA-10 Leydig cells. We characterized the transcription initiation site and promoter of the Acsl4 mouse gene and identified three alternative splice variants present in MA-10 cells. Sequence analysis of a 1.5-kb fragment of the Acsl4 promoter revealed the absence of a TATA box and the presence of many putative binding sites for transcription factors including Sp1 and CREB. Functional characterization revealed that the specificity protein/Krüppel-like factor Sp1 binding site in the proximal promoter is involved in basal activity and that the cAMP response element-binding site is involved in cAMP stimulation of Acsl4 transcription.
publishDate 2013
dc.date.none.fl_str_mv 2013-04
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/8523
Orlando, Ulises Daniel; Cooke, Mariana; Cornejo Maciel, Fabiana; Papadopoulos, Vassilios; Podesta, Ernesto Jorge; et al.; Characterization of the mouse promoter region of the acyl-CoA synthetase 4 gene: Role of Sp1 and CREB ; Elsevier Ireland; Molecular And Cellular Endocrinology; 369; 1-2; 4-2013; 15-26
0303-7207
url http://hdl.handle.net/11336/8523
identifier_str_mv Orlando, Ulises Daniel; Cooke, Mariana; Cornejo Maciel, Fabiana; Papadopoulos, Vassilios; Podesta, Ernesto Jorge; et al.; Characterization of the mouse promoter region of the acyl-CoA synthetase 4 gene: Role of Sp1 and CREB ; Elsevier Ireland; Molecular And Cellular Endocrinology; 369; 1-2; 4-2013; 15-26
0303-7207
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/http://www.sciencedirect.com/science/article/pii/S0303720713000397
info:eu-repo/semantics/altIdentifier/doi/10.1016/j.mce.2013.01.016
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Elsevier Ireland
publisher.none.fl_str_mv Elsevier Ireland
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
_version_ 1846782164755021824
score 12.8982525

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