Molecular evaluation of Chagas disease reactivation and treatment follow-up in HIV coinfected patients

Autores
Fernandez, Marisa; Besuschio, Susana; Nicita, D.; Latini, V.; Biondi, M. L.; García, J.; Corti, Marcelo; Schijman, Alejandro Gabriel; Burgos, J.
Año de publicación
2018
Idioma
inglés
Tipo de recurso
documento de conferencia
Estado
versión publicada
Descripción
Background: Chagas disease reactivation is an AIDS defined illness that usually affects Central Nervous System. Gold-Standard diagnosis for T.cruzi reactivation is based on microscopical observation methods.Methods & Materials: Seven patients with HIV/AIDS diagnosis, T.cruzi serological findings, neurological disorders, and suspected of Chagas disease reactivation were included between 2015?2017 from two health centers of Buenos Aires, Argentina. Real-time PCRs (qPCR) against T.cruzi satellite DNA were carried out from cerebrospinal fluid (CSF) and peripheral blood samples (BS) for parasite load quantification. Molecular parasite characterization was based on amplification of spliced leader intergenic region, 24 srDNA, and A10 polymorphic sequences.Results: Patients were aged from 41 to 69 years old, 43% were women, CD4+ T cell counts were between 7 and 53 cell/mm3. All of them received tripanocidal treatment (TrypT).Five CSFs were withdrawn before TrypT starting. Two had microscopical detection of trypomastigote forms (MDTryp) and quantification over 500 p.e/mL by qPCR. The other 3 CSF samples were non-detectable by both methods. The remaining two patients CSF were obtained after starting TrypT with MDTryp negative findings but qPCR positive results with parasite burden below 13 p.e/mL.Among the 3 patients with negative CSF findings, 2 had positive MDTryp on BS with 119 and 3512 p.e/mL. The third was negative by MDTryp and had 2 p.e/mL before TrypT.All patients had qPCR positive findings on BS (2 -1426 p.e/mL) and decreased their parasitic loads during TrypT.All characterized parasites from BS and CSF samples belonged to DTU (Discrete Typing Unit) II, V or VI, frequently found in Southern Cone region.Out of this small series, 4 patients died: 2 because of brain ?Chagoma?, 1 due to status epilepticus and 1 due to acute abdomen. Two of the 3 survivors were those with negative CSF findings for both methods.Conclusion: Chagas disease reactivation occurred on deep inmunosupressed HIV people. Its diagnosis by MDTryp and qPCR is complementary. qPCR detected T.cruzi DNA on negative MDTryp findings even during treatment. TrypT always reduces T.cruzi DNA loads. qPCR might be an useful therapeutical marker.
Fil: Fernandez, Marisa. Gobierno de la Ciudad de Buenos Aires. Hospital de Infecciosas "Dr. Francisco Javier Muñiz"; Argentina
Fil: Besuschio, Susana. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigaciones en Ingeniería Genética y Biología Molecular "Dr. Héctor N. Torres"; Argentina
Fil: Nicita, D.. Gobierno de la Ciudad de Buenos Aires. Hospital de Infecciosas "Dr. Francisco Javier Muñiz"; Argentina
Fil: Latini, V.. Sanatorio de la Trinidad Mitre; Argentina
Fil: Biondi, M. L.. Gobierno de la Ciudad de Buenos Aires. Hospital de Infecciosas "Dr. Francisco Javier Muñiz"; Argentina
Fil: García, J.. Gobierno de la Ciudad de Buenos Aires. Hospital de Infecciosas "Dr. Francisco Javier Muñiz"; Argentina
Fil: Corti, Marcelo. Gobierno de la Ciudad de Buenos Aires. Hospital de Infecciosas "Dr. Francisco Javier Muñiz"; Argentina
Fil: Schijman, Alejandro Gabriel. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigaciones en Ingeniería Genética y Biología Molecular "Dr. Héctor N. Torres"; Argentina
Fil: Burgos, J.. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mar del Plata. Instituto de Investigaciones Biológicas. Universidad Nacional de Mar del Plata. Facultad de Ciencias Exactas y Naturales. Instituto de Investigaciones Biológicas; Argentina
18th International Congress on Infectious Diseases (ICID)
Ciudad Autónoma de Buenos Aires
Argentina
International Society fot Infectious Diseases
Materia
HIV
Chagas
Molecular diagnosis
Reactivation
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/137283
Acceder
id CONICETDig_5719efd101baf5f427435bf433e8e81f
oai_identifier_str oai:ri.conicet.gov.ar:11336/137283
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Molecular evaluation of Chagas disease reactivation and treatment follow-up in HIV coinfected patientsFernandez, MarisaBesuschio, SusanaNicita, D.Latini, V.Biondi, M. L.García, J.Corti, MarceloSchijman, Alejandro GabrielBurgos, J.HIVChagasMolecular diagnosisReactivationhttps://purl.org/becyt/ford/3.3https://purl.org/becyt/ford/3Background: Chagas disease reactivation is an AIDS defined illness that usually affects Central Nervous System. Gold-Standard diagnosis for T.cruzi reactivation is based on microscopical observation methods.Methods & Materials: Seven patients with HIV/AIDS diagnosis, T.cruzi serological findings, neurological disorders, and suspected of Chagas disease reactivation were included between 2015?2017 from two health centers of Buenos Aires, Argentina. Real-time PCRs (qPCR) against T.cruzi satellite DNA were carried out from cerebrospinal fluid (CSF) and peripheral blood samples (BS) for parasite load quantification. Molecular parasite characterization was based on amplification of spliced leader intergenic region, 24 srDNA, and A10 polymorphic sequences.Results: Patients were aged from 41 to 69 years old, 43% were women, CD4+ T cell counts were between 7 and 53 cell/mm3. All of them received tripanocidal treatment (TrypT).Five CSFs were withdrawn before TrypT starting. Two had microscopical detection of trypomastigote forms (MDTryp) and quantification over 500 p.e/mL by qPCR. The other 3 CSF samples were non-detectable by both methods. The remaining two patients CSF were obtained after starting TrypT with MDTryp negative findings but qPCR positive results with parasite burden below 13 p.e/mL.Among the 3 patients with negative CSF findings, 2 had positive MDTryp on BS with 119 and 3512 p.e/mL. The third was negative by MDTryp and had 2 p.e/mL before TrypT.All patients had qPCR positive findings on BS (2 -1426 p.e/mL) and decreased their parasitic loads during TrypT.All characterized parasites from BS and CSF samples belonged to DTU (Discrete Typing Unit) II, V or VI, frequently found in Southern Cone region.Out of this small series, 4 patients died: 2 because of brain ?Chagoma?, 1 due to status epilepticus and 1 due to acute abdomen. Two of the 3 survivors were those with negative CSF findings for both methods.Conclusion: Chagas disease reactivation occurred on deep inmunosupressed HIV people. Its diagnosis by MDTryp and qPCR is complementary. qPCR detected T.cruzi DNA on negative MDTryp findings even during treatment. TrypT always reduces T.cruzi DNA loads. qPCR might be an useful therapeutical marker.Fil: Fernandez, Marisa. Gobierno de la Ciudad de Buenos Aires. Hospital de Infecciosas "Dr. Francisco Javier Muñiz"; ArgentinaFil: Besuschio, Susana. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigaciones en Ingeniería Genética y Biología Molecular "Dr. Héctor N. Torres"; ArgentinaFil: Nicita, D.. Gobierno de la Ciudad de Buenos Aires. Hospital de Infecciosas "Dr. Francisco Javier Muñiz"; ArgentinaFil: Latini, V.. Sanatorio de la Trinidad Mitre; ArgentinaFil: Biondi, M. L.. Gobierno de la Ciudad de Buenos Aires. Hospital de Infecciosas "Dr. Francisco Javier Muñiz"; ArgentinaFil: García, J.. Gobierno de la Ciudad de Buenos Aires. Hospital de Infecciosas "Dr. Francisco Javier Muñiz"; ArgentinaFil: Corti, Marcelo. Gobierno de la Ciudad de Buenos Aires. Hospital de Infecciosas "Dr. Francisco Javier Muñiz"; ArgentinaFil: Schijman, Alejandro Gabriel. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigaciones en Ingeniería Genética y Biología Molecular "Dr. Héctor N. Torres"; ArgentinaFil: Burgos, J.. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mar del Plata. Instituto de Investigaciones Biológicas. Universidad Nacional de Mar del Plata. Facultad de Ciencias Exactas y Naturales. Instituto de Investigaciones Biológicas; Argentina18th International Congress on Infectious Diseases (ICID)Ciudad Autónoma de Buenos AiresArgentinaInternational Society fot Infectious DiseasesElsevier2018info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/conferenceObjectCongresoJournalhttp://purl.org/coar/resource_type/c_5794info:ar-repo/semantics/documentoDeConferenciaapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/137283Molecular evaluation of Chagas disease reactivation and treatment follow-up in HIV coinfected patients; 18th International Congress on Infectious Diseases (ICID); Ciudad Autónoma de Buenos Aires; Argentina; 2018; 322-3220002-9637CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://doi.org/10.1016/j.ijid.2018.04.4147info:eu-repo/semantics/altIdentifier/url/https://www.ijidonline.com/article/S1201-9712(18)34231-0/fulltextInternacionalinfo:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-10-15T15:16:15Zoai:ri.conicet.gov.ar:11336/137283instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-10-15 15:16:15.399CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Molecular evaluation of Chagas disease reactivation and treatment follow-up in HIV coinfected patients
title Molecular evaluation of Chagas disease reactivation and treatment follow-up in HIV coinfected patients
spellingShingle Molecular evaluation of Chagas disease reactivation and treatment follow-up in HIV coinfected patients
Fernandez, Marisa
HIV
Chagas
Molecular diagnosis
Reactivation
title_short Molecular evaluation of Chagas disease reactivation and treatment follow-up in HIV coinfected patients
title_full Molecular evaluation of Chagas disease reactivation and treatment follow-up in HIV coinfected patients
title_fullStr Molecular evaluation of Chagas disease reactivation and treatment follow-up in HIV coinfected patients
title_full_unstemmed Molecular evaluation of Chagas disease reactivation and treatment follow-up in HIV coinfected patients
title_sort Molecular evaluation of Chagas disease reactivation and treatment follow-up in HIV coinfected patients
dc.creator.none.fl_str_mv Fernandez, Marisa
Besuschio, Susana
Nicita, D.
Latini, V.
Biondi, M. L.
García, J.
Corti, Marcelo
Schijman, Alejandro Gabriel
Burgos, J.
author Fernandez, Marisa
author_facet Fernandez, Marisa
Besuschio, Susana
Nicita, D.
Latini, V.
Biondi, M. L.
García, J.
Corti, Marcelo
Schijman, Alejandro Gabriel
Burgos, J.
author_role author
author2 Besuschio, Susana
Nicita, D.
Latini, V.
Biondi, M. L.
García, J.
Corti, Marcelo
Schijman, Alejandro Gabriel
Burgos, J.
author2_role author
author
author
author
author
author
author
author
dc.subject.none.fl_str_mv HIV
Chagas
Molecular diagnosis
Reactivation
topic HIV
Chagas
Molecular diagnosis
Reactivation
purl_subject.fl_str_mv https://purl.org/becyt/ford/3.3
https://purl.org/becyt/ford/3
dc.description.none.fl_txt_mv Background: Chagas disease reactivation is an AIDS defined illness that usually affects Central Nervous System. Gold-Standard diagnosis for T.cruzi reactivation is based on microscopical observation methods.Methods & Materials: Seven patients with HIV/AIDS diagnosis, T.cruzi serological findings, neurological disorders, and suspected of Chagas disease reactivation were included between 2015?2017 from two health centers of Buenos Aires, Argentina. Real-time PCRs (qPCR) against T.cruzi satellite DNA were carried out from cerebrospinal fluid (CSF) and peripheral blood samples (BS) for parasite load quantification. Molecular parasite characterization was based on amplification of spliced leader intergenic region, 24 srDNA, and A10 polymorphic sequences.Results: Patients were aged from 41 to 69 years old, 43% were women, CD4+ T cell counts were between 7 and 53 cell/mm3. All of them received tripanocidal treatment (TrypT).Five CSFs were withdrawn before TrypT starting. Two had microscopical detection of trypomastigote forms (MDTryp) and quantification over 500 p.e/mL by qPCR. The other 3 CSF samples were non-detectable by both methods. The remaining two patients CSF were obtained after starting TrypT with MDTryp negative findings but qPCR positive results with parasite burden below 13 p.e/mL.Among the 3 patients with negative CSF findings, 2 had positive MDTryp on BS with 119 and 3512 p.e/mL. The third was negative by MDTryp and had 2 p.e/mL before TrypT.All patients had qPCR positive findings on BS (2 -1426 p.e/mL) and decreased their parasitic loads during TrypT.All characterized parasites from BS and CSF samples belonged to DTU (Discrete Typing Unit) II, V or VI, frequently found in Southern Cone region.Out of this small series, 4 patients died: 2 because of brain ?Chagoma?, 1 due to status epilepticus and 1 due to acute abdomen. Two of the 3 survivors were those with negative CSF findings for both methods.Conclusion: Chagas disease reactivation occurred on deep inmunosupressed HIV people. Its diagnosis by MDTryp and qPCR is complementary. qPCR detected T.cruzi DNA on negative MDTryp findings even during treatment. TrypT always reduces T.cruzi DNA loads. qPCR might be an useful therapeutical marker.
Fil: Fernandez, Marisa. Gobierno de la Ciudad de Buenos Aires. Hospital de Infecciosas "Dr. Francisco Javier Muñiz"; Argentina
Fil: Besuschio, Susana. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigaciones en Ingeniería Genética y Biología Molecular "Dr. Héctor N. Torres"; Argentina
Fil: Nicita, D.. Gobierno de la Ciudad de Buenos Aires. Hospital de Infecciosas "Dr. Francisco Javier Muñiz"; Argentina
Fil: Latini, V.. Sanatorio de la Trinidad Mitre; Argentina
Fil: Biondi, M. L.. Gobierno de la Ciudad de Buenos Aires. Hospital de Infecciosas "Dr. Francisco Javier Muñiz"; Argentina
Fil: García, J.. Gobierno de la Ciudad de Buenos Aires. Hospital de Infecciosas "Dr. Francisco Javier Muñiz"; Argentina
Fil: Corti, Marcelo. Gobierno de la Ciudad de Buenos Aires. Hospital de Infecciosas "Dr. Francisco Javier Muñiz"; Argentina
Fil: Schijman, Alejandro Gabriel. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigaciones en Ingeniería Genética y Biología Molecular "Dr. Héctor N. Torres"; Argentina
Fil: Burgos, J.. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mar del Plata. Instituto de Investigaciones Biológicas. Universidad Nacional de Mar del Plata. Facultad de Ciencias Exactas y Naturales. Instituto de Investigaciones Biológicas; Argentina
18th International Congress on Infectious Diseases (ICID)
Ciudad Autónoma de Buenos Aires
Argentina
International Society fot Infectious Diseases
description Background: Chagas disease reactivation is an AIDS defined illness that usually affects Central Nervous System. Gold-Standard diagnosis for T.cruzi reactivation is based on microscopical observation methods.Methods & Materials: Seven patients with HIV/AIDS diagnosis, T.cruzi serological findings, neurological disorders, and suspected of Chagas disease reactivation were included between 2015?2017 from two health centers of Buenos Aires, Argentina. Real-time PCRs (qPCR) against T.cruzi satellite DNA were carried out from cerebrospinal fluid (CSF) and peripheral blood samples (BS) for parasite load quantification. Molecular parasite characterization was based on amplification of spliced leader intergenic region, 24 srDNA, and A10 polymorphic sequences.Results: Patients were aged from 41 to 69 years old, 43% were women, CD4+ T cell counts were between 7 and 53 cell/mm3. All of them received tripanocidal treatment (TrypT).Five CSFs were withdrawn before TrypT starting. Two had microscopical detection of trypomastigote forms (MDTryp) and quantification over 500 p.e/mL by qPCR. The other 3 CSF samples were non-detectable by both methods. The remaining two patients CSF were obtained after starting TrypT with MDTryp negative findings but qPCR positive results with parasite burden below 13 p.e/mL.Among the 3 patients with negative CSF findings, 2 had positive MDTryp on BS with 119 and 3512 p.e/mL. The third was negative by MDTryp and had 2 p.e/mL before TrypT.All patients had qPCR positive findings on BS (2 -1426 p.e/mL) and decreased their parasitic loads during TrypT.All characterized parasites from BS and CSF samples belonged to DTU (Discrete Typing Unit) II, V or VI, frequently found in Southern Cone region.Out of this small series, 4 patients died: 2 because of brain ?Chagoma?, 1 due to status epilepticus and 1 due to acute abdomen. Two of the 3 survivors were those with negative CSF findings for both methods.Conclusion: Chagas disease reactivation occurred on deep inmunosupressed HIV people. Its diagnosis by MDTryp and qPCR is complementary. qPCR detected T.cruzi DNA on negative MDTryp findings even during treatment. TrypT always reduces T.cruzi DNA loads. qPCR might be an useful therapeutical marker.
publishDate 2018
dc.date.none.fl_str_mv 2018
dc.type.none.fl_str_mv info:eu-repo/semantics/publishedVersion
info:eu-repo/semantics/conferenceObject
Congreso
Journal
http://purl.org/coar/resource_type/c_5794
info:ar-repo/semantics/documentoDeConferencia
status_str publishedVersion
format conferenceObject
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/137283
Molecular evaluation of Chagas disease reactivation and treatment follow-up in HIV coinfected patients; 18th International Congress on Infectious Diseases (ICID); Ciudad Autónoma de Buenos Aires; Argentina; 2018; 322-322
0002-9637
CONICET Digital
CONICET
url http://hdl.handle.net/11336/137283
identifier_str_mv Molecular evaluation of Chagas disease reactivation and treatment follow-up in HIV coinfected patients; 18th International Congress on Infectious Diseases (ICID); Ciudad Autónoma de Buenos Aires; Argentina; 2018; 322-322
0002-9637
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://doi.org/10.1016/j.ijid.2018.04.4147
info:eu-repo/semantics/altIdentifier/url/https://www.ijidonline.com/article/S1201-9712(18)34231-0/fulltext
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.coverage.none.fl_str_mv Internacional
dc.publisher.none.fl_str_mv Elsevier
publisher.none.fl_str_mv Elsevier
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
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reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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