Closing the gaps to understand the tick transmission of Anaplasma marginale among giant anteaters (Myrmecophaga tridactyla) in Argentina

Autores
Guillemi, Eliana Carolina; Imbert, Mélody; de la Fourniere, Sofía Ana María; Orozco, Maria Marcela; Peña Martínez, Jorge; Rosas, Ana Carolina; Montenegro, Valeria Noely; Farber, Marisa Diana
Año de publicación
2020
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Anaplasma marginale, a well-known cattle pathogen of tropical and subtropical world regions, has been previously molecularly characterized in a giant anteater (Myrmecophaga tridactyla) from Corrientes, Argentina. Ticks or other hematophagous arthropod involved in the wild transmission cycle remained unknown. The aim of the present study was to analyze the simultaneous occurrence of A. marginale in blood samples and ticks from giant anteaters from Corrientes in order to investigate if ticks could be relevant in the transmission among these mammals. Blood samples from 50 giant anteaters collected in different years and 26 ticks Amblyomma dubitatum and A. sculptum were studied through the molecular amplification of two unequivocal species-specific genes from A. marginale: msp5 and msp1β. Twenty five giant anteaters and tick organs (salivary glands, gut and oviduct) from 11 ticks tested positive to the A. marginale DNA amplification. The further molecular characterization through MSP1a tandem repeats analysis revealed the presence of genotypes circulating among giant anteaters that had been previously identified in cattle blood samples from the same geographical region. These results confirm the presence of A. marginale in giant anteaters in Corrientes and suggests that A. dubitatum and A. sculptum ticks could be involved in the transmission among giant anteaters. Future studies will determine the role of these tick species in the wild transmission cycle in the study area and the eventual connection with the domestic cycle.
Fil: Guillemi, Eliana Carolina. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigación en Ciencias Veterinarias y Agronómicas. Instituto de Agrobiotecnología y Biología Molecular. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Agrobiotecnología y Biología Molecular; Argentina
Fil: Imbert, Mélody. Université Paul Sabatier; Francia
Fil: de la Fourniere, Sofía Ana María. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigación en Ciencias Veterinarias y Agronómicas. Instituto de Agrobiotecnología y Biología Molecular. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Agrobiotecnología y Biología Molecular; Argentina
Fil: Orozco, Maria Marcela. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Ecología, Genética y Evolución de Buenos Aires. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Instituto de Ecología, Genética y Evolución de Buenos Aires; Argentina
Fil: Peña Martínez, Jorge. The Conservation Land Trust; Argentina
Fil: Rosas, Ana Carolina. The Conservation Land Trust; Argentina
Fil: Montenegro, Valeria Noely. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigación en Ciencias Veterinarias y Agronómicas. Instituto de Agrobiotecnología y Biología Molecular. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Agrobiotecnología y Biología Molecular; Argentina
Fil: Farber, Marisa Diana. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigación en Ciencias Veterinarias y Agronómicas. Instituto de Agrobiotecnología y Biología Molecular. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Agrobiotecnología y Biología Molecular; Argentina
Materia
A. SCULPTUM
AMBLYOMMA DUBITATUM
ANAPLASMA MARGINALE
GIANT ANTEATER
MYRMECOPHAGA TRIDACTYLA
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/142415
Acceder
id CONICETDig_5711ece054d56c5a872fcd75ca81bce9
oai_identifier_str oai:ri.conicet.gov.ar:11336/142415
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Closing the gaps to understand the tick transmission of Anaplasma marginale among giant anteaters (Myrmecophaga tridactyla) in ArgentinaGuillemi, Eliana CarolinaImbert, Mélodyde la Fourniere, Sofía Ana MaríaOrozco, Maria MarcelaPeña Martínez, JorgeRosas, Ana CarolinaMontenegro, Valeria NoelyFarber, Marisa DianaA. SCULPTUMAMBLYOMMA DUBITATUMANAPLASMA MARGINALEGIANT ANTEATERMYRMECOPHAGA TRIDACTYLAhttps://purl.org/becyt/ford/4.3https://purl.org/becyt/ford/4Anaplasma marginale, a well-known cattle pathogen of tropical and subtropical world regions, has been previously molecularly characterized in a giant anteater (Myrmecophaga tridactyla) from Corrientes, Argentina. Ticks or other hematophagous arthropod involved in the wild transmission cycle remained unknown. The aim of the present study was to analyze the simultaneous occurrence of A. marginale in blood samples and ticks from giant anteaters from Corrientes in order to investigate if ticks could be relevant in the transmission among these mammals. Blood samples from 50 giant anteaters collected in different years and 26 ticks Amblyomma dubitatum and A. sculptum were studied through the molecular amplification of two unequivocal species-specific genes from A. marginale: msp5 and msp1β. Twenty five giant anteaters and tick organs (salivary glands, gut and oviduct) from 11 ticks tested positive to the A. marginale DNA amplification. The further molecular characterization through MSP1a tandem repeats analysis revealed the presence of genotypes circulating among giant anteaters that had been previously identified in cattle blood samples from the same geographical region. These results confirm the presence of A. marginale in giant anteaters in Corrientes and suggests that A. dubitatum and A. sculptum ticks could be involved in the transmission among giant anteaters. Future studies will determine the role of these tick species in the wild transmission cycle in the study area and the eventual connection with the domestic cycle.Fil: Guillemi, Eliana Carolina. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigación en Ciencias Veterinarias y Agronómicas. Instituto de Agrobiotecnología y Biología Molecular. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Agrobiotecnología y Biología Molecular; ArgentinaFil: Imbert, Mélody. Université Paul Sabatier; FranciaFil: de la Fourniere, Sofía Ana María. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigación en Ciencias Veterinarias y Agronómicas. Instituto de Agrobiotecnología y Biología Molecular. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Agrobiotecnología y Biología Molecular; ArgentinaFil: Orozco, Maria Marcela. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Ecología, Genética y Evolución de Buenos Aires. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Instituto de Ecología, Genética y Evolución de Buenos Aires; ArgentinaFil: Peña Martínez, Jorge. The Conservation Land Trust; ArgentinaFil: Rosas, Ana Carolina. The Conservation Land Trust; ArgentinaFil: Montenegro, Valeria Noely. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigación en Ciencias Veterinarias y Agronómicas. Instituto de Agrobiotecnología y Biología Molecular. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Agrobiotecnología y Biología Molecular; ArgentinaFil: Farber, Marisa Diana. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigación en Ciencias Veterinarias y Agronómicas. Instituto de Agrobiotecnología y Biología Molecular. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Agrobiotecnología y Biología Molecular; ArgentinaMolecular Diversity Preservation International2020-12info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/142415Guillemi, Eliana Carolina; Imbert, Mélody; de la Fourniere, Sofía Ana María; Orozco, Maria Marcela; Peña Martínez, Jorge; et al.; Closing the gaps to understand the tick transmission of Anaplasma marginale among giant anteaters (Myrmecophaga tridactyla) in Argentina; Molecular Diversity Preservation International; Pathogens; 9; 12; 12-2020; 1-82076-0817CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://www.mdpi.com/2076-0817/9/12/1033info:eu-repo/semantics/altIdentifier/doi/10.3390/pathogens9121033info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-03T10:09:36Zoai:ri.conicet.gov.ar:11336/142415instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-03 10:09:36.436CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Closing the gaps to understand the tick transmission of Anaplasma marginale among giant anteaters (Myrmecophaga tridactyla) in Argentina
title Closing the gaps to understand the tick transmission of Anaplasma marginale among giant anteaters (Myrmecophaga tridactyla) in Argentina
spellingShingle Closing the gaps to understand the tick transmission of Anaplasma marginale among giant anteaters (Myrmecophaga tridactyla) in Argentina
Guillemi, Eliana Carolina
A. SCULPTUM
AMBLYOMMA DUBITATUM
ANAPLASMA MARGINALE
GIANT ANTEATER
MYRMECOPHAGA TRIDACTYLA
title_short Closing the gaps to understand the tick transmission of Anaplasma marginale among giant anteaters (Myrmecophaga tridactyla) in Argentina
title_full Closing the gaps to understand the tick transmission of Anaplasma marginale among giant anteaters (Myrmecophaga tridactyla) in Argentina
title_fullStr Closing the gaps to understand the tick transmission of Anaplasma marginale among giant anteaters (Myrmecophaga tridactyla) in Argentina
title_full_unstemmed Closing the gaps to understand the tick transmission of Anaplasma marginale among giant anteaters (Myrmecophaga tridactyla) in Argentina
title_sort Closing the gaps to understand the tick transmission of Anaplasma marginale among giant anteaters (Myrmecophaga tridactyla) in Argentina
dc.creator.none.fl_str_mv Guillemi, Eliana Carolina
Imbert, Mélody
de la Fourniere, Sofía Ana María
Orozco, Maria Marcela
Peña Martínez, Jorge
Rosas, Ana Carolina
Montenegro, Valeria Noely
Farber, Marisa Diana
author Guillemi, Eliana Carolina
author_facet Guillemi, Eliana Carolina
Imbert, Mélody
de la Fourniere, Sofía Ana María
Orozco, Maria Marcela
Peña Martínez, Jorge
Rosas, Ana Carolina
Montenegro, Valeria Noely
Farber, Marisa Diana
author_role author
author2 Imbert, Mélody
de la Fourniere, Sofía Ana María
Orozco, Maria Marcela
Peña Martínez, Jorge
Rosas, Ana Carolina
Montenegro, Valeria Noely
Farber, Marisa Diana
author2_role author
author
author
author
author
author
author
dc.subject.none.fl_str_mv A. SCULPTUM
AMBLYOMMA DUBITATUM
ANAPLASMA MARGINALE
GIANT ANTEATER
MYRMECOPHAGA TRIDACTYLA
topic A. SCULPTUM
AMBLYOMMA DUBITATUM
ANAPLASMA MARGINALE
GIANT ANTEATER
MYRMECOPHAGA TRIDACTYLA
purl_subject.fl_str_mv https://purl.org/becyt/ford/4.3
https://purl.org/becyt/ford/4
dc.description.none.fl_txt_mv Anaplasma marginale, a well-known cattle pathogen of tropical and subtropical world regions, has been previously molecularly characterized in a giant anteater (Myrmecophaga tridactyla) from Corrientes, Argentina. Ticks or other hematophagous arthropod involved in the wild transmission cycle remained unknown. The aim of the present study was to analyze the simultaneous occurrence of A. marginale in blood samples and ticks from giant anteaters from Corrientes in order to investigate if ticks could be relevant in the transmission among these mammals. Blood samples from 50 giant anteaters collected in different years and 26 ticks Amblyomma dubitatum and A. sculptum were studied through the molecular amplification of two unequivocal species-specific genes from A. marginale: msp5 and msp1β. Twenty five giant anteaters and tick organs (salivary glands, gut and oviduct) from 11 ticks tested positive to the A. marginale DNA amplification. The further molecular characterization through MSP1a tandem repeats analysis revealed the presence of genotypes circulating among giant anteaters that had been previously identified in cattle blood samples from the same geographical region. These results confirm the presence of A. marginale in giant anteaters in Corrientes and suggests that A. dubitatum and A. sculptum ticks could be involved in the transmission among giant anteaters. Future studies will determine the role of these tick species in the wild transmission cycle in the study area and the eventual connection with the domestic cycle.
Fil: Guillemi, Eliana Carolina. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigación en Ciencias Veterinarias y Agronómicas. Instituto de Agrobiotecnología y Biología Molecular. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Agrobiotecnología y Biología Molecular; Argentina
Fil: Imbert, Mélody. Université Paul Sabatier; Francia
Fil: de la Fourniere, Sofía Ana María. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigación en Ciencias Veterinarias y Agronómicas. Instituto de Agrobiotecnología y Biología Molecular. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Agrobiotecnología y Biología Molecular; Argentina
Fil: Orozco, Maria Marcela. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Ecología, Genética y Evolución de Buenos Aires. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Instituto de Ecología, Genética y Evolución de Buenos Aires; Argentina
Fil: Peña Martínez, Jorge. The Conservation Land Trust; Argentina
Fil: Rosas, Ana Carolina. The Conservation Land Trust; Argentina
Fil: Montenegro, Valeria Noely. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigación en Ciencias Veterinarias y Agronómicas. Instituto de Agrobiotecnología y Biología Molecular. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Agrobiotecnología y Biología Molecular; Argentina
Fil: Farber, Marisa Diana. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigación en Ciencias Veterinarias y Agronómicas. Instituto de Agrobiotecnología y Biología Molecular. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Agrobiotecnología y Biología Molecular; Argentina
description Anaplasma marginale, a well-known cattle pathogen of tropical and subtropical world regions, has been previously molecularly characterized in a giant anteater (Myrmecophaga tridactyla) from Corrientes, Argentina. Ticks or other hematophagous arthropod involved in the wild transmission cycle remained unknown. The aim of the present study was to analyze the simultaneous occurrence of A. marginale in blood samples and ticks from giant anteaters from Corrientes in order to investigate if ticks could be relevant in the transmission among these mammals. Blood samples from 50 giant anteaters collected in different years and 26 ticks Amblyomma dubitatum and A. sculptum were studied through the molecular amplification of two unequivocal species-specific genes from A. marginale: msp5 and msp1β. Twenty five giant anteaters and tick organs (salivary glands, gut and oviduct) from 11 ticks tested positive to the A. marginale DNA amplification. The further molecular characterization through MSP1a tandem repeats analysis revealed the presence of genotypes circulating among giant anteaters that had been previously identified in cattle blood samples from the same geographical region. These results confirm the presence of A. marginale in giant anteaters in Corrientes and suggests that A. dubitatum and A. sculptum ticks could be involved in the transmission among giant anteaters. Future studies will determine the role of these tick species in the wild transmission cycle in the study area and the eventual connection with the domestic cycle.
publishDate 2020
dc.date.none.fl_str_mv 2020-12
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/142415
Guillemi, Eliana Carolina; Imbert, Mélody; de la Fourniere, Sofía Ana María; Orozco, Maria Marcela; Peña Martínez, Jorge; et al.; Closing the gaps to understand the tick transmission of Anaplasma marginale among giant anteaters (Myrmecophaga tridactyla) in Argentina; Molecular Diversity Preservation International; Pathogens; 9; 12; 12-2020; 1-8
2076-0817
CONICET Digital
CONICET
url http://hdl.handle.net/11336/142415
identifier_str_mv Guillemi, Eliana Carolina; Imbert, Mélody; de la Fourniere, Sofía Ana María; Orozco, Maria Marcela; Peña Martínez, Jorge; et al.; Closing the gaps to understand the tick transmission of Anaplasma marginale among giant anteaters (Myrmecophaga tridactyla) in Argentina; Molecular Diversity Preservation International; Pathogens; 9; 12; 12-2020; 1-8
2076-0817
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://www.mdpi.com/2076-0817/9/12/1033
info:eu-repo/semantics/altIdentifier/doi/10.3390/pathogens9121033
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Molecular Diversity Preservation International
publisher.none.fl_str_mv Molecular Diversity Preservation International
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.13397

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