Sucrose may play a different role other than osmoprotectant in Synechocystis sp. PCC 6803 salt-shock cells
- Autores
- Desplats, Paula; Folco, Maria Esther; Salerno, Graciela Lidia
- Año de publicación
- 2005
- Idioma
- inglés
- Tipo de recurso
- artículo
- Estado
- versión publicada
- Descripción
- The role of sucrose in cyanobacteria is still not fully understood. It is generally considered a salt-response molecule, and particularly, in Synechocystis sp. strain PCC 6803, it is referred as a secondary osmolyte. We showed that sucrose accumulates transiently in Synechocystis cells at early stages of a salt shock, which could be ascribed to salt activation of sucrose-phosphate synthase (SPS, UDP-glucose d-fructose-6-phosphate 2-α-d-glucosyltransferase; EC 2.4.1.14), the key enzyme in sucrose synthesis pathway, and to an increase of the expression of the SPS encoding gene. Experiments with a mutant strain impaired in sucrose biosynthesis showed that sucrose is essential in stationary phase cells to overcome a later salt stress. Taken together, these results led us to suggest a more intricate function for sucrose than to be an osmoprotectant compound.
Fil: Desplats, Paula. Fundación para Investigaciones Biológicas Aplicadas; Argentina
Fil: Folco, Maria Esther. Fundación para Investigaciones Biológicas Aplicadas; Argentina
Fil: Salerno, Graciela Lidia. Fundación para Investigaciones Biológicas Aplicadas; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mar del Plata; Argentina - Materia
-
CYANOBACTERIUM
SALT STRESS
SUCROSE
SUCROSE-PHOSPHATE SYNTHASE
SYNECHOCYSTIS SP. PCC 6803 - Nivel de accesibilidad
- acceso abierto
- Condiciones de uso
- https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
- Repositorio
- Institución
- Consejo Nacional de Investigaciones Científicas y Técnicas
- OAI Identificador
- oai:ri.conicet.gov.ar:11336/132908
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Sucrose may play a different role other than osmoprotectant in Synechocystis sp. PCC 6803 salt-shock cellsDesplats, PaulaFolco, Maria EstherSalerno, Graciela LidiaCYANOBACTERIUMSALT STRESSSUCROSESUCROSE-PHOSPHATE SYNTHASESYNECHOCYSTIS SP. PCC 6803https://purl.org/becyt/ford/1.4https://purl.org/becyt/ford/1The role of sucrose in cyanobacteria is still not fully understood. It is generally considered a salt-response molecule, and particularly, in Synechocystis sp. strain PCC 6803, it is referred as a secondary osmolyte. We showed that sucrose accumulates transiently in Synechocystis cells at early stages of a salt shock, which could be ascribed to salt activation of sucrose-phosphate synthase (SPS, UDP-glucose d-fructose-6-phosphate 2-α-d-glucosyltransferase; EC 2.4.1.14), the key enzyme in sucrose synthesis pathway, and to an increase of the expression of the SPS encoding gene. Experiments with a mutant strain impaired in sucrose biosynthesis showed that sucrose is essential in stationary phase cells to overcome a later salt stress. Taken together, these results led us to suggest a more intricate function for sucrose than to be an osmoprotectant compound.Fil: Desplats, Paula. Fundación para Investigaciones Biológicas Aplicadas; ArgentinaFil: Folco, Maria Esther. Fundación para Investigaciones Biológicas Aplicadas; ArgentinaFil: Salerno, Graciela Lidia. Fundación para Investigaciones Biológicas Aplicadas; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mar del Plata; ArgentinaElsevier France-editions Scientifiques Medicales Elsevier2005-02info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/132908Desplats, Paula; Folco, Maria Esther; Salerno, Graciela Lidia; Sucrose may play a different role other than osmoprotectant in Synechocystis sp. PCC 6803 salt-shock cells; Elsevier France-editions Scientifiques Medicales Elsevier; Plant Physiology and Biochemistry; 43; 2; 2-2005; 133-1380981-9428CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.1016/j.plaphy.2005.01.008info:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/abs/pii/S0981942805000173info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-03T09:46:28Zoai:ri.conicet.gov.ar:11336/132908instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-03 09:46:29.12CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse |
dc.title.none.fl_str_mv |
Sucrose may play a different role other than osmoprotectant in Synechocystis sp. PCC 6803 salt-shock cells |
title |
Sucrose may play a different role other than osmoprotectant in Synechocystis sp. PCC 6803 salt-shock cells |
spellingShingle |
Sucrose may play a different role other than osmoprotectant in Synechocystis sp. PCC 6803 salt-shock cells Desplats, Paula CYANOBACTERIUM SALT STRESS SUCROSE SUCROSE-PHOSPHATE SYNTHASE SYNECHOCYSTIS SP. PCC 6803 |
title_short |
Sucrose may play a different role other than osmoprotectant in Synechocystis sp. PCC 6803 salt-shock cells |
title_full |
Sucrose may play a different role other than osmoprotectant in Synechocystis sp. PCC 6803 salt-shock cells |
title_fullStr |
Sucrose may play a different role other than osmoprotectant in Synechocystis sp. PCC 6803 salt-shock cells |
title_full_unstemmed |
Sucrose may play a different role other than osmoprotectant in Synechocystis sp. PCC 6803 salt-shock cells |
title_sort |
Sucrose may play a different role other than osmoprotectant in Synechocystis sp. PCC 6803 salt-shock cells |
dc.creator.none.fl_str_mv |
Desplats, Paula Folco, Maria Esther Salerno, Graciela Lidia |
author |
Desplats, Paula |
author_facet |
Desplats, Paula Folco, Maria Esther Salerno, Graciela Lidia |
author_role |
author |
author2 |
Folco, Maria Esther Salerno, Graciela Lidia |
author2_role |
author author |
dc.subject.none.fl_str_mv |
CYANOBACTERIUM SALT STRESS SUCROSE SUCROSE-PHOSPHATE SYNTHASE SYNECHOCYSTIS SP. PCC 6803 |
topic |
CYANOBACTERIUM SALT STRESS SUCROSE SUCROSE-PHOSPHATE SYNTHASE SYNECHOCYSTIS SP. PCC 6803 |
purl_subject.fl_str_mv |
https://purl.org/becyt/ford/1.4 https://purl.org/becyt/ford/1 |
dc.description.none.fl_txt_mv |
The role of sucrose in cyanobacteria is still not fully understood. It is generally considered a salt-response molecule, and particularly, in Synechocystis sp. strain PCC 6803, it is referred as a secondary osmolyte. We showed that sucrose accumulates transiently in Synechocystis cells at early stages of a salt shock, which could be ascribed to salt activation of sucrose-phosphate synthase (SPS, UDP-glucose d-fructose-6-phosphate 2-α-d-glucosyltransferase; EC 2.4.1.14), the key enzyme in sucrose synthesis pathway, and to an increase of the expression of the SPS encoding gene. Experiments with a mutant strain impaired in sucrose biosynthesis showed that sucrose is essential in stationary phase cells to overcome a later salt stress. Taken together, these results led us to suggest a more intricate function for sucrose than to be an osmoprotectant compound. Fil: Desplats, Paula. Fundación para Investigaciones Biológicas Aplicadas; Argentina Fil: Folco, Maria Esther. Fundación para Investigaciones Biológicas Aplicadas; Argentina Fil: Salerno, Graciela Lidia. Fundación para Investigaciones Biológicas Aplicadas; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mar del Plata; Argentina |
description |
The role of sucrose in cyanobacteria is still not fully understood. It is generally considered a salt-response molecule, and particularly, in Synechocystis sp. strain PCC 6803, it is referred as a secondary osmolyte. We showed that sucrose accumulates transiently in Synechocystis cells at early stages of a salt shock, which could be ascribed to salt activation of sucrose-phosphate synthase (SPS, UDP-glucose d-fructose-6-phosphate 2-α-d-glucosyltransferase; EC 2.4.1.14), the key enzyme in sucrose synthesis pathway, and to an increase of the expression of the SPS encoding gene. Experiments with a mutant strain impaired in sucrose biosynthesis showed that sucrose is essential in stationary phase cells to overcome a later salt stress. Taken together, these results led us to suggest a more intricate function for sucrose than to be an osmoprotectant compound. |
publishDate |
2005 |
dc.date.none.fl_str_mv |
2005-02 |
dc.type.none.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion http://purl.org/coar/resource_type/c_6501 info:ar-repo/semantics/articulo |
format |
article |
status_str |
publishedVersion |
dc.identifier.none.fl_str_mv |
http://hdl.handle.net/11336/132908 Desplats, Paula; Folco, Maria Esther; Salerno, Graciela Lidia; Sucrose may play a different role other than osmoprotectant in Synechocystis sp. PCC 6803 salt-shock cells; Elsevier France-editions Scientifiques Medicales Elsevier; Plant Physiology and Biochemistry; 43; 2; 2-2005; 133-138 0981-9428 CONICET Digital CONICET |
url |
http://hdl.handle.net/11336/132908 |
identifier_str_mv |
Desplats, Paula; Folco, Maria Esther; Salerno, Graciela Lidia; Sucrose may play a different role other than osmoprotectant in Synechocystis sp. PCC 6803 salt-shock cells; Elsevier France-editions Scientifiques Medicales Elsevier; Plant Physiology and Biochemistry; 43; 2; 2-2005; 133-138 0981-9428 CONICET Digital CONICET |
dc.language.none.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
info:eu-repo/semantics/altIdentifier/doi/10.1016/j.plaphy.2005.01.008 info:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/abs/pii/S0981942805000173 |
dc.rights.none.fl_str_mv |
info:eu-repo/semantics/openAccess https://creativecommons.org/licenses/by-nc-sa/2.5/ar/ |
eu_rights_str_mv |
openAccess |
rights_invalid_str_mv |
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/ |
dc.format.none.fl_str_mv |
application/pdf application/pdf |
dc.publisher.none.fl_str_mv |
Elsevier France-editions Scientifiques Medicales Elsevier |
publisher.none.fl_str_mv |
Elsevier France-editions Scientifiques Medicales Elsevier |
dc.source.none.fl_str_mv |
reponame:CONICET Digital (CONICET) instname:Consejo Nacional de Investigaciones Científicas y Técnicas |
reponame_str |
CONICET Digital (CONICET) |
collection |
CONICET Digital (CONICET) |
instname_str |
Consejo Nacional de Investigaciones Científicas y Técnicas |
repository.name.fl_str_mv |
CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas |
repository.mail.fl_str_mv |
dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar |
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1842268797565665280 |
score |
13.13397 |