Sucrose may play a different role other than osmoprotectant in Synechocystis sp. PCC 6803 salt-shock cells

Autores
Desplats, Paula; Folco, Maria Esther; Salerno, Graciela Lidia
Año de publicación
2005
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
The role of sucrose in cyanobacteria is still not fully understood. It is generally considered a salt-response molecule, and particularly, in Synechocystis sp. strain PCC 6803, it is referred as a secondary osmolyte. We showed that sucrose accumulates transiently in Synechocystis cells at early stages of a salt shock, which could be ascribed to salt activation of sucrose-phosphate synthase (SPS, UDP-glucose d-fructose-6-phosphate 2-α-d-glucosyltransferase; EC 2.4.1.14), the key enzyme in sucrose synthesis pathway, and to an increase of the expression of the SPS encoding gene. Experiments with a mutant strain impaired in sucrose biosynthesis showed that sucrose is essential in stationary phase cells to overcome a later salt stress. Taken together, these results led us to suggest a more intricate function for sucrose than to be an osmoprotectant compound.
Fil: Desplats, Paula. Fundación para Investigaciones Biológicas Aplicadas; Argentina
Fil: Folco, Maria Esther. Fundación para Investigaciones Biológicas Aplicadas; Argentina
Fil: Salerno, Graciela Lidia. Fundación para Investigaciones Biológicas Aplicadas; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mar del Plata; Argentina
Materia
CYANOBACTERIUM
SALT STRESS
SUCROSE
SUCROSE-PHOSPHATE SYNTHASE
SYNECHOCYSTIS SP. PCC 6803
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/132908
Acceder
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network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Sucrose may play a different role other than osmoprotectant in Synechocystis sp. PCC 6803 salt-shock cellsDesplats, PaulaFolco, Maria EstherSalerno, Graciela LidiaCYANOBACTERIUMSALT STRESSSUCROSESUCROSE-PHOSPHATE SYNTHASESYNECHOCYSTIS SP. PCC 6803https://purl.org/becyt/ford/1.4https://purl.org/becyt/ford/1The role of sucrose in cyanobacteria is still not fully understood. It is generally considered a salt-response molecule, and particularly, in Synechocystis sp. strain PCC 6803, it is referred as a secondary osmolyte. We showed that sucrose accumulates transiently in Synechocystis cells at early stages of a salt shock, which could be ascribed to salt activation of sucrose-phosphate synthase (SPS, UDP-glucose d-fructose-6-phosphate 2-α-d-glucosyltransferase; EC 2.4.1.14), the key enzyme in sucrose synthesis pathway, and to an increase of the expression of the SPS encoding gene. Experiments with a mutant strain impaired in sucrose biosynthesis showed that sucrose is essential in stationary phase cells to overcome a later salt stress. Taken together, these results led us to suggest a more intricate function for sucrose than to be an osmoprotectant compound.Fil: Desplats, Paula. Fundación para Investigaciones Biológicas Aplicadas; ArgentinaFil: Folco, Maria Esther. Fundación para Investigaciones Biológicas Aplicadas; ArgentinaFil: Salerno, Graciela Lidia. Fundación para Investigaciones Biológicas Aplicadas; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mar del Plata; ArgentinaElsevier France-editions Scientifiques Medicales Elsevier2005-02info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/132908Desplats, Paula; Folco, Maria Esther; Salerno, Graciela Lidia; Sucrose may play a different role other than osmoprotectant in Synechocystis sp. PCC 6803 salt-shock cells; Elsevier France-editions Scientifiques Medicales Elsevier; Plant Physiology and Biochemistry; 43; 2; 2-2005; 133-1380981-9428CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.1016/j.plaphy.2005.01.008info:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/abs/pii/S0981942805000173info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T09:37:43Zoai:ri.conicet.gov.ar:11336/132908instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 09:37:43.61CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Sucrose may play a different role other than osmoprotectant in Synechocystis sp. PCC 6803 salt-shock cells
title Sucrose may play a different role other than osmoprotectant in Synechocystis sp. PCC 6803 salt-shock cells
spellingShingle Sucrose may play a different role other than osmoprotectant in Synechocystis sp. PCC 6803 salt-shock cells
Desplats, Paula
CYANOBACTERIUM
SALT STRESS
SUCROSE
SUCROSE-PHOSPHATE SYNTHASE
SYNECHOCYSTIS SP. PCC 6803
title_short Sucrose may play a different role other than osmoprotectant in Synechocystis sp. PCC 6803 salt-shock cells
title_full Sucrose may play a different role other than osmoprotectant in Synechocystis sp. PCC 6803 salt-shock cells
title_fullStr Sucrose may play a different role other than osmoprotectant in Synechocystis sp. PCC 6803 salt-shock cells
title_full_unstemmed Sucrose may play a different role other than osmoprotectant in Synechocystis sp. PCC 6803 salt-shock cells
title_sort Sucrose may play a different role other than osmoprotectant in Synechocystis sp. PCC 6803 salt-shock cells
dc.creator.none.fl_str_mv Desplats, Paula
Folco, Maria Esther
Salerno, Graciela Lidia
author Desplats, Paula
author_facet Desplats, Paula
Folco, Maria Esther
Salerno, Graciela Lidia
author_role author
author2 Folco, Maria Esther
Salerno, Graciela Lidia
author2_role author
author
dc.subject.none.fl_str_mv CYANOBACTERIUM
SALT STRESS
SUCROSE
SUCROSE-PHOSPHATE SYNTHASE
SYNECHOCYSTIS SP. PCC 6803
topic CYANOBACTERIUM
SALT STRESS
SUCROSE
SUCROSE-PHOSPHATE SYNTHASE
SYNECHOCYSTIS SP. PCC 6803
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.4
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv The role of sucrose in cyanobacteria is still not fully understood. It is generally considered a salt-response molecule, and particularly, in Synechocystis sp. strain PCC 6803, it is referred as a secondary osmolyte. We showed that sucrose accumulates transiently in Synechocystis cells at early stages of a salt shock, which could be ascribed to salt activation of sucrose-phosphate synthase (SPS, UDP-glucose d-fructose-6-phosphate 2-α-d-glucosyltransferase; EC 2.4.1.14), the key enzyme in sucrose synthesis pathway, and to an increase of the expression of the SPS encoding gene. Experiments with a mutant strain impaired in sucrose biosynthesis showed that sucrose is essential in stationary phase cells to overcome a later salt stress. Taken together, these results led us to suggest a more intricate function for sucrose than to be an osmoprotectant compound.
Fil: Desplats, Paula. Fundación para Investigaciones Biológicas Aplicadas; Argentina
Fil: Folco, Maria Esther. Fundación para Investigaciones Biológicas Aplicadas; Argentina
Fil: Salerno, Graciela Lidia. Fundación para Investigaciones Biológicas Aplicadas; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mar del Plata; Argentina
description The role of sucrose in cyanobacteria is still not fully understood. It is generally considered a salt-response molecule, and particularly, in Synechocystis sp. strain PCC 6803, it is referred as a secondary osmolyte. We showed that sucrose accumulates transiently in Synechocystis cells at early stages of a salt shock, which could be ascribed to salt activation of sucrose-phosphate synthase (SPS, UDP-glucose d-fructose-6-phosphate 2-α-d-glucosyltransferase; EC 2.4.1.14), the key enzyme in sucrose synthesis pathway, and to an increase of the expression of the SPS encoding gene. Experiments with a mutant strain impaired in sucrose biosynthesis showed that sucrose is essential in stationary phase cells to overcome a later salt stress. Taken together, these results led us to suggest a more intricate function for sucrose than to be an osmoprotectant compound.
publishDate 2005
dc.date.none.fl_str_mv 2005-02
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/132908
Desplats, Paula; Folco, Maria Esther; Salerno, Graciela Lidia; Sucrose may play a different role other than osmoprotectant in Synechocystis sp. PCC 6803 salt-shock cells; Elsevier France-editions Scientifiques Medicales Elsevier; Plant Physiology and Biochemistry; 43; 2; 2-2005; 133-138
0981-9428
CONICET Digital
CONICET
url http://hdl.handle.net/11336/132908
identifier_str_mv Desplats, Paula; Folco, Maria Esther; Salerno, Graciela Lidia; Sucrose may play a different role other than osmoprotectant in Synechocystis sp. PCC 6803 salt-shock cells; Elsevier France-editions Scientifiques Medicales Elsevier; Plant Physiology and Biochemistry; 43; 2; 2-2005; 133-138
0981-9428
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.1016/j.plaphy.2005.01.008
info:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/abs/pii/S0981942805000173
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Elsevier France-editions Scientifiques Medicales Elsevier
publisher.none.fl_str_mv Elsevier France-editions Scientifiques Medicales Elsevier
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.070432

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