A Neospora caninum vaccine using recombinant proteins fails to prevent foetal infection in pregnant cattle after experimental intravenous challenge

Autores
Hecker, Yanina Paola; Cóceres, Verónica Mabel; Wilkowsky, Silvina Elizabeth; Jaramillo Ortiz, José Manuel; Morrell, Eleonora Lidia; Verna, Andrea Elizabeth; Ganuza, Agustina; Cano, Dora B.; Lischinsky, Lilian; Ángel, Sergio Oscar; Zamorano, Patricia Ines; Odeón, Anselmo Carlos; Leunda, Maria Rosa; Campero, Carlos Manuel; Morein, Bror; Moore, Dadin Prando
Año de publicación
2014
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
The aim of the present study was to evaluate the immunogenicity and protective efficacy of rNcSAG1, rNcHSP20, rNcGRA7 recombinant proteins formulated with immune stimulating complexes (ISCOMs) in pregnant heifers against vertical transmission of N. caninum. Twelve pregnant heifers were divided into 3 groups of 4 heifers each, receiving different formulations before mating. Immunogens were administered twice subcutaneously: group A animals were inoculated with three recombinant proteins (rNcSAG1, rNcHSP20, rNcGRA7) formulated with ISCOMs; group B animals received ISCOM-MATRIX (without antigen) and group C received sterile phosphate-buffered saline (PBS) only. The recombinant proteins were expressed in Escherichia coli and purified by nickel resin. All groups were intravenously challenged with the NC-1 strain of Neospora caninum at day 70 of gestation and dams slaughtered at week 17 of the experiment. Heifers from group A developed specific antibodies against rNcSAG1, rNcHSP20 and rNcGRA7 prior to the challenge. Following immunization, an statistically significant increase of antibodies against rNcSAG1 and rNcHSP20 in all animals of group A was detected compared to animals in groups B and C at weeks 5, 13 and 16 (P <0.001). Levels of antibodies against rNcGRA7 were statistical higher in group A animals when compared with groups B and C at weeks 5 and 16 (P > 0.001). There were no differences in IFN-γ production among the experimental groups at any time point (P > 0.05). Transplacental transmission was determined in all fetuses of groups A, B and C by Western Blot, Immunohistochemistry and nested PCR. This work showed that rNcSAG1, rNcHSP20 and rNcGRA7 proteins while immunogenic in cattle failed to prevent the fetal infection in pregnant cattle challenged at Day 70 of gestation.
Fil: Hecker, Yanina Paola. Instituto Nacional de Tecnología Agropecuaria. Centro Regional Buenos Aires Sur. Estación Experimental Agropecuaria Balcarce; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Cóceres, Verónica Mabel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Investigaciones Biotecnológicas. Instituto de Investigaciones Biotecnológicas ; Argentina
Fil: Wilkowsky, Silvina Elizabeth. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Instituto Nacional de Tecnología Agropecuaria; Argentina
Fil: Jaramillo Ortiz, José Manuel. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Instituto Nacional de Tecnología Agropecuaria; Argentina
Fil: Morrell, Eleonora Lidia. Instituto Nacional de Tecnología Agropecuaria; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Verna, Andrea Elizabeth. Instituto Nacional de Tecnología Agropecuaria; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Ganuza, Agustina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Investigaciones Biotecnológicas. Instituto de Investigaciones Biotecnológicas "Dr. Raúl Alfonsín" (sede Chascomús). Universidad Nacional de San Martín. Instituto de Investigaciones Biotecnológicas. Instituto de Investigaciones Biotecnológicas "Dr. Raúl Alfonsín" (sede Chascomús); Argentina
Fil: Cano, Dora B.. Instituto Nacional de Tecnología Agropecuaria; Argentina
Fil: Lischinsky, Lilian. Instituto Nacional de Tecnología Agropecuaria; Argentina
Fil: Ángel, Sergio Oscar. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Investigaciones Biotecnológicas. Instituto de Investigaciones Biotecnológicas "Dr. Raúl Alfonsín" (sede Chascomús). Universidad Nacional de San Martín. Instituto de Investigaciones Biotecnológicas. Instituto de Investigaciones Biotecnológicas "Dr. Raúl Alfonsín" (sede Chascomús); Argentina
Fil: Zamorano, Patricia Ines. Instituto Nacional de Tecnología Agropecuaria; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Odeón, Anselmo Carlos. Instituto Nacional de Tecnología Agropecuaria; Argentina
Fil: Leunda, Maria Rosa. Instituto Nacional de Tecnología Agropecuaria; Argentina
Fil: Campero, Carlos Manuel. Instituto Nacional de Tecnología Agropecuaria; Argentina
Fil: Morein, Bror. Uppsala University. Department of Medical Sciences. Infectious Diseases; Suecia
Fil: Moore, Dadin Prando. Instituto Nacional de Tecnología Agropecuaria; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Materia
Neospora Caninum
Vaccine
Rncsag1
Rnchsp20
Rncgra7
Iscoms
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/33656
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/33656
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling A Neospora caninum vaccine using recombinant proteins fails to prevent foetal infection in pregnant cattle after experimental intravenous challengeHecker, Yanina PaolaCóceres, Verónica MabelWilkowsky, Silvina ElizabethJaramillo Ortiz, José ManuelMorrell, Eleonora LidiaVerna, Andrea ElizabethGanuza, AgustinaCano, Dora B.Lischinsky, LilianÁngel, Sergio OscarZamorano, Patricia InesOdeón, Anselmo CarlosLeunda, Maria RosaCampero, Carlos ManuelMorein, BrorMoore, Dadin PrandoNeospora CaninumVaccineRncsag1Rnchsp20Rncgra7Iscomshttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1The aim of the present study was to evaluate the immunogenicity and protective efficacy of rNcSAG1, rNcHSP20, rNcGRA7 recombinant proteins formulated with immune stimulating complexes (ISCOMs) in pregnant heifers against vertical transmission of N. caninum. Twelve pregnant heifers were divided into 3 groups of 4 heifers each, receiving different formulations before mating. Immunogens were administered twice subcutaneously: group A animals were inoculated with three recombinant proteins (rNcSAG1, rNcHSP20, rNcGRA7) formulated with ISCOMs; group B animals received ISCOM-MATRIX (without antigen) and group C received sterile phosphate-buffered saline (PBS) only. The recombinant proteins were expressed in Escherichia coli and purified by nickel resin. All groups were intravenously challenged with the NC-1 strain of Neospora caninum at day 70 of gestation and dams slaughtered at week 17 of the experiment. Heifers from group A developed specific antibodies against rNcSAG1, rNcHSP20 and rNcGRA7 prior to the challenge. Following immunization, an statistically significant increase of antibodies against rNcSAG1 and rNcHSP20 in all animals of group A was detected compared to animals in groups B and C at weeks 5, 13 and 16 (P <0.001). Levels of antibodies against rNcGRA7 were statistical higher in group A animals when compared with groups B and C at weeks 5 and 16 (P > 0.001). There were no differences in IFN-γ production among the experimental groups at any time point (P > 0.05). Transplacental transmission was determined in all fetuses of groups A, B and C by Western Blot, Immunohistochemistry and nested PCR. This work showed that rNcSAG1, rNcHSP20 and rNcGRA7 proteins while immunogenic in cattle failed to prevent the fetal infection in pregnant cattle challenged at Day 70 of gestation.Fil: Hecker, Yanina Paola. Instituto Nacional de Tecnología Agropecuaria. Centro Regional Buenos Aires Sur. Estación Experimental Agropecuaria Balcarce; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Cóceres, Verónica Mabel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Investigaciones Biotecnológicas. Instituto de Investigaciones Biotecnológicas ; ArgentinaFil: Wilkowsky, Silvina Elizabeth. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Instituto Nacional de Tecnología Agropecuaria; ArgentinaFil: Jaramillo Ortiz, José Manuel. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Instituto Nacional de Tecnología Agropecuaria; ArgentinaFil: Morrell, Eleonora Lidia. Instituto Nacional de Tecnología Agropecuaria; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Verna, Andrea Elizabeth. Instituto Nacional de Tecnología Agropecuaria; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Ganuza, Agustina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Investigaciones Biotecnológicas. Instituto de Investigaciones Biotecnológicas "Dr. Raúl Alfonsín" (sede Chascomús). Universidad Nacional de San Martín. Instituto de Investigaciones Biotecnológicas. Instituto de Investigaciones Biotecnológicas "Dr. Raúl Alfonsín" (sede Chascomús); ArgentinaFil: Cano, Dora B.. Instituto Nacional de Tecnología Agropecuaria; ArgentinaFil: Lischinsky, Lilian. Instituto Nacional de Tecnología Agropecuaria; ArgentinaFil: Ángel, Sergio Oscar. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Investigaciones Biotecnológicas. Instituto de Investigaciones Biotecnológicas "Dr. Raúl Alfonsín" (sede Chascomús). Universidad Nacional de San Martín. Instituto de Investigaciones Biotecnológicas. Instituto de Investigaciones Biotecnológicas "Dr. Raúl Alfonsín" (sede Chascomús); ArgentinaFil: Zamorano, Patricia Ines. Instituto Nacional de Tecnología Agropecuaria; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Odeón, Anselmo Carlos. Instituto Nacional de Tecnología Agropecuaria; ArgentinaFil: Leunda, Maria Rosa. Instituto Nacional de Tecnología Agropecuaria; ArgentinaFil: Campero, Carlos Manuel. Instituto Nacional de Tecnología Agropecuaria; ArgentinaFil: Morein, Bror. Uppsala University. Department of Medical Sciences. Infectious Diseases; SueciaFil: Moore, Dadin Prando. Instituto Nacional de Tecnología Agropecuaria; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaElsevier2014-11info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/33656Morrell, Eleonora Lidia; Ángel, Sergio Oscar; Ganuza, Agustina; Jaramillo Ortiz, José Manuel; Campero, Carlos Manuel; Lischinsky, Lilian; A Neospora caninum vaccine using recombinant proteins fails to prevent foetal infection in pregnant cattle after experimental intravenous challenge; Elsevier; Veterinary Immunology And Immunopathology; 162; 3-4; 11-2014; 142-1530165-2427CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.1016/j.vetimm.2014.11.001info:eu-repo/semantics/altIdentifier/url/http://www.sciencedirect.com/science/article/pii/S016524271400258Xinfo:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-10T13:14:58Zoai:ri.conicet.gov.ar:11336/33656instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-10 13:14:58.866CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv A Neospora caninum vaccine using recombinant proteins fails to prevent foetal infection in pregnant cattle after experimental intravenous challenge
title A Neospora caninum vaccine using recombinant proteins fails to prevent foetal infection in pregnant cattle after experimental intravenous challenge
spellingShingle A Neospora caninum vaccine using recombinant proteins fails to prevent foetal infection in pregnant cattle after experimental intravenous challenge
Hecker, Yanina Paola
Neospora Caninum
Vaccine
Rncsag1
Rnchsp20
Rncgra7
Iscoms
title_short A Neospora caninum vaccine using recombinant proteins fails to prevent foetal infection in pregnant cattle after experimental intravenous challenge
title_full A Neospora caninum vaccine using recombinant proteins fails to prevent foetal infection in pregnant cattle after experimental intravenous challenge
title_fullStr A Neospora caninum vaccine using recombinant proteins fails to prevent foetal infection in pregnant cattle after experimental intravenous challenge
title_full_unstemmed A Neospora caninum vaccine using recombinant proteins fails to prevent foetal infection in pregnant cattle after experimental intravenous challenge
title_sort A Neospora caninum vaccine using recombinant proteins fails to prevent foetal infection in pregnant cattle after experimental intravenous challenge
dc.creator.none.fl_str_mv Hecker, Yanina Paola
Cóceres, Verónica Mabel
Wilkowsky, Silvina Elizabeth
Jaramillo Ortiz, José Manuel
Morrell, Eleonora Lidia
Verna, Andrea Elizabeth
Ganuza, Agustina
Cano, Dora B.
Lischinsky, Lilian
Ángel, Sergio Oscar
Zamorano, Patricia Ines
Odeón, Anselmo Carlos
Leunda, Maria Rosa
Campero, Carlos Manuel
Morein, Bror
Moore, Dadin Prando
author Hecker, Yanina Paola
author_facet Hecker, Yanina Paola
Cóceres, Verónica Mabel
Wilkowsky, Silvina Elizabeth
Jaramillo Ortiz, José Manuel
Morrell, Eleonora Lidia
Verna, Andrea Elizabeth
Ganuza, Agustina
Cano, Dora B.
Lischinsky, Lilian
Ángel, Sergio Oscar
Zamorano, Patricia Ines
Odeón, Anselmo Carlos
Leunda, Maria Rosa
Campero, Carlos Manuel
Morein, Bror
Moore, Dadin Prando
author_role author
author2 Cóceres, Verónica Mabel
Wilkowsky, Silvina Elizabeth
Jaramillo Ortiz, José Manuel
Morrell, Eleonora Lidia
Verna, Andrea Elizabeth
Ganuza, Agustina
Cano, Dora B.
Lischinsky, Lilian
Ángel, Sergio Oscar
Zamorano, Patricia Ines
Odeón, Anselmo Carlos
Leunda, Maria Rosa
Campero, Carlos Manuel
Morein, Bror
Moore, Dadin Prando
author2_role author
author
author
author
author
author
author
author
author
author
author
author
author
author
author
dc.subject.none.fl_str_mv Neospora Caninum
Vaccine
Rncsag1
Rnchsp20
Rncgra7
Iscoms
topic Neospora Caninum
Vaccine
Rncsag1
Rnchsp20
Rncgra7
Iscoms
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv The aim of the present study was to evaluate the immunogenicity and protective efficacy of rNcSAG1, rNcHSP20, rNcGRA7 recombinant proteins formulated with immune stimulating complexes (ISCOMs) in pregnant heifers against vertical transmission of N. caninum. Twelve pregnant heifers were divided into 3 groups of 4 heifers each, receiving different formulations before mating. Immunogens were administered twice subcutaneously: group A animals were inoculated with three recombinant proteins (rNcSAG1, rNcHSP20, rNcGRA7) formulated with ISCOMs; group B animals received ISCOM-MATRIX (without antigen) and group C received sterile phosphate-buffered saline (PBS) only. The recombinant proteins were expressed in Escherichia coli and purified by nickel resin. All groups were intravenously challenged with the NC-1 strain of Neospora caninum at day 70 of gestation and dams slaughtered at week 17 of the experiment. Heifers from group A developed specific antibodies against rNcSAG1, rNcHSP20 and rNcGRA7 prior to the challenge. Following immunization, an statistically significant increase of antibodies against rNcSAG1 and rNcHSP20 in all animals of group A was detected compared to animals in groups B and C at weeks 5, 13 and 16 (P <0.001). Levels of antibodies against rNcGRA7 were statistical higher in group A animals when compared with groups B and C at weeks 5 and 16 (P > 0.001). There were no differences in IFN-γ production among the experimental groups at any time point (P > 0.05). Transplacental transmission was determined in all fetuses of groups A, B and C by Western Blot, Immunohistochemistry and nested PCR. This work showed that rNcSAG1, rNcHSP20 and rNcGRA7 proteins while immunogenic in cattle failed to prevent the fetal infection in pregnant cattle challenged at Day 70 of gestation.
Fil: Hecker, Yanina Paola. Instituto Nacional de Tecnología Agropecuaria. Centro Regional Buenos Aires Sur. Estación Experimental Agropecuaria Balcarce; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Cóceres, Verónica Mabel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Investigaciones Biotecnológicas. Instituto de Investigaciones Biotecnológicas ; Argentina
Fil: Wilkowsky, Silvina Elizabeth. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Instituto Nacional de Tecnología Agropecuaria; Argentina
Fil: Jaramillo Ortiz, José Manuel. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Instituto Nacional de Tecnología Agropecuaria; Argentina
Fil: Morrell, Eleonora Lidia. Instituto Nacional de Tecnología Agropecuaria; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Verna, Andrea Elizabeth. Instituto Nacional de Tecnología Agropecuaria; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Ganuza, Agustina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Investigaciones Biotecnológicas. Instituto de Investigaciones Biotecnológicas "Dr. Raúl Alfonsín" (sede Chascomús). Universidad Nacional de San Martín. Instituto de Investigaciones Biotecnológicas. Instituto de Investigaciones Biotecnológicas "Dr. Raúl Alfonsín" (sede Chascomús); Argentina
Fil: Cano, Dora B.. Instituto Nacional de Tecnología Agropecuaria; Argentina
Fil: Lischinsky, Lilian. Instituto Nacional de Tecnología Agropecuaria; Argentina
Fil: Ángel, Sergio Oscar. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Investigaciones Biotecnológicas. Instituto de Investigaciones Biotecnológicas "Dr. Raúl Alfonsín" (sede Chascomús). Universidad Nacional de San Martín. Instituto de Investigaciones Biotecnológicas. Instituto de Investigaciones Biotecnológicas "Dr. Raúl Alfonsín" (sede Chascomús); Argentina
Fil: Zamorano, Patricia Ines. Instituto Nacional de Tecnología Agropecuaria; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Odeón, Anselmo Carlos. Instituto Nacional de Tecnología Agropecuaria; Argentina
Fil: Leunda, Maria Rosa. Instituto Nacional de Tecnología Agropecuaria; Argentina
Fil: Campero, Carlos Manuel. Instituto Nacional de Tecnología Agropecuaria; Argentina
Fil: Morein, Bror. Uppsala University. Department of Medical Sciences. Infectious Diseases; Suecia
Fil: Moore, Dadin Prando. Instituto Nacional de Tecnología Agropecuaria; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
description The aim of the present study was to evaluate the immunogenicity and protective efficacy of rNcSAG1, rNcHSP20, rNcGRA7 recombinant proteins formulated with immune stimulating complexes (ISCOMs) in pregnant heifers against vertical transmission of N. caninum. Twelve pregnant heifers were divided into 3 groups of 4 heifers each, receiving different formulations before mating. Immunogens were administered twice subcutaneously: group A animals were inoculated with three recombinant proteins (rNcSAG1, rNcHSP20, rNcGRA7) formulated with ISCOMs; group B animals received ISCOM-MATRIX (without antigen) and group C received sterile phosphate-buffered saline (PBS) only. The recombinant proteins were expressed in Escherichia coli and purified by nickel resin. All groups were intravenously challenged with the NC-1 strain of Neospora caninum at day 70 of gestation and dams slaughtered at week 17 of the experiment. Heifers from group A developed specific antibodies against rNcSAG1, rNcHSP20 and rNcGRA7 prior to the challenge. Following immunization, an statistically significant increase of antibodies against rNcSAG1 and rNcHSP20 in all animals of group A was detected compared to animals in groups B and C at weeks 5, 13 and 16 (P <0.001). Levels of antibodies against rNcGRA7 were statistical higher in group A animals when compared with groups B and C at weeks 5 and 16 (P > 0.001). There were no differences in IFN-γ production among the experimental groups at any time point (P > 0.05). Transplacental transmission was determined in all fetuses of groups A, B and C by Western Blot, Immunohistochemistry and nested PCR. This work showed that rNcSAG1, rNcHSP20 and rNcGRA7 proteins while immunogenic in cattle failed to prevent the fetal infection in pregnant cattle challenged at Day 70 of gestation.
publishDate 2014
dc.date.none.fl_str_mv 2014-11
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/33656
Morrell, Eleonora Lidia; Ángel, Sergio Oscar; Ganuza, Agustina; Jaramillo Ortiz, José Manuel; Campero, Carlos Manuel; Lischinsky, Lilian; A Neospora caninum vaccine using recombinant proteins fails to prevent foetal infection in pregnant cattle after experimental intravenous challenge; Elsevier; Veterinary Immunology And Immunopathology; 162; 3-4; 11-2014; 142-153
0165-2427
CONICET Digital
CONICET
url http://hdl.handle.net/11336/33656
identifier_str_mv Morrell, Eleonora Lidia; Ángel, Sergio Oscar; Ganuza, Agustina; Jaramillo Ortiz, José Manuel; Campero, Carlos Manuel; Lischinsky, Lilian; A Neospora caninum vaccine using recombinant proteins fails to prevent foetal infection in pregnant cattle after experimental intravenous challenge; Elsevier; Veterinary Immunology And Immunopathology; 162; 3-4; 11-2014; 142-153
0165-2427
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.1016/j.vetimm.2014.11.001
info:eu-repo/semantics/altIdentifier/url/http://www.sciencedirect.com/science/article/pii/S016524271400258X
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
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dc.publisher.none.fl_str_mv Elsevier
publisher.none.fl_str_mv Elsevier
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
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reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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